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31.
H Ikemoto K Watanabe T Mori J Igari T Oguri Y Shimizu T Terai H Inoue T Nakadate C Ito T Yoshida I Ohno Y Tanno M Arakawa K Igarashi M Okada K Ozaki N Aoki N Kitamura O Sekine Y Suzuki K Nakata T Nakatani H Inagawa N Kusano 《Canadian Metallurgical Quarterly》1998,51(7):437-474
The bacteria isolated from the patients with lower respiratory tract infections were collected by institutions located throughout Japan, since 1981. Ikemoto et al. have been investigating susceptibilities of these isolates to various antibacterial agents and antibiotics, and characteristics of the patients and isolates from them each year. Results obtained from these investigations are discussed. In 16 institutions around the entire Japan, 557 strains of presumably etiological bacteria were isolated mainly from the sputa of 449 patients with lower respiratory tract infections during the period from October 1996 to September 1997. MICs of various antibacterial agents and antibiotics were determined against 98 strains of Staphylococcus aureus, 93 strains of Streptococcus pneumoniae, 84 strains of Haemophilus influenzae, 84 strains of Pseudomonas aeruginosa (non-mucoid strains), 17 strains of Pseudomonas aeruginosa (mucoid strains), 31 strains of Moraxella subgenus Branhamella catarrhalis, 21 strains of Klebsiella pneumoniae etc., and the drug susceptibilities of these strains were assessed except for those strains that died during transportation. 1) S. aureus S. aureus strains for which MICs of oxacillin (MPIPC) were higher than 4 micrograms/ml (methicillin-resistant S. aureus) accounted for 67.3%. The frequency of the drug resistant bacteria increased comparing to the previous year's 52.7%. Arbekacin (ABK) and vancomycin (VCM) showed the highest activities against both S. aureus and MRSA with MIC80s of 1 microgram/ml. 2) S. pneumoniae Imipenem (IPM) and panipenem (PAPM) of carbapenems showed the most potent activities with MIC80s of 0.063 microgram/ml. Faropenem (FRPM) showed the next potent activity with MIC80 of 0.125 microgram/ml. The other drugs except erythromycin (EM), clindamycin (CLDM) and tetracycline (TC) were active against S. pneumoniae tested with MIC80s of 8 micrograms/ml or below. 3) H. influenzae The activities of all drugs were potent against H. influenzae tested with MIC80s of 4 micrograms/ml or below. Cefotiam (CTM), cefmenoxime (CMX), cefditoren (CDTR) and ofloxacin (OFLX) showed the most potent activities with MIC80s of 0.063 microgram/ml. 4) P. aeruginosa (mucoid strains) Tobramycin (TOB) showed the most potent activity against P. aeruginosa (mucoid strains) with MIC80 of 1 microgram/ml. Ceftazidime (CAZ), cefsulodin (CFS), IPM, gentamicin (GM), ABK and ciprofloxacin (CPFX) showed the next potent activities, with MIC80s of 2 micrograms/ml. The MIC80s of the other drugs ranged from 4 micrograms/ml to 16 micrograms/ml. 5) P. aeruginosa (non-mucoid strains) TOB and CPFX showed the most potent activities against P. aeruginosa (non-mucoid strains) with MIC80s of 1 microgram/ml. The MIC80s of piperacillin (PIPC) and cefoperazone (CPZ) were 16 micrograms/ml in 1995, and they were 64 micrograms/ml in 1996. 6) K. pneumoniae All drugs except ampicillin (ABPC) were active against K. pneumoniae. CMX, cefpirome (CPR), cefozopran (CZOP) and carumonam (CRMN) showed the most potent activities against K. pneumoniae with MIC80s of 0.125 microgram/ml. The MIC80s of the other drugs ranged from 0.25 microgram/ml to 2 micrograms/ml. 7) M.(B) catarrhalis Against M.(B.) catarrhalis, all drugs showed good activities with MICs of 4 micrograms/ml or below. IPM and minocycline (MINO) showed the most potent activities with MICs of 0.063 microgram/ml. Also, we investigated year to year changes in the characteristics of patients, their respiratory infectious diseases, and the etiology. Patients' backgrounds were examined for 557 isolates from 449 cases. The examination of age distribution indicated that the proportion of patients with ages over 60 years was 71.0% of all the patients showing a slight increase over that in 1994. Proportions of diagnosed diseases were as follows: Bacterial pneumonia and chronic bronchitis were the most frequent with 35.9% and 30.3% respectively. They were followed by bronchiectasis with a proportion of 10. 相似文献
32.
N Keicho K Tokunaga K Nakata Y Taguchi A Azuma M Bannai M Emi N Ohishi Y Yazaki S Kudoh 《Canadian Metallurgical Quarterly》1998,158(3):846-850
Diffuse panbronchiolitis (DPB) in East Asia is a distinctive chronic obstructive pulmonary disease of unknown etiology. We hypothesize that the disease susceptibility is due to genetic predisposition unique to Asians. Association between human leukocyte antigen (HLA)-Bw54 and the disease was previously reported. In the present study, using newly developed polymerase chain reaction (PCR)- based methods, we directly analyzed HLA class I and II alleles in 76 Japanese patients. HLA-A, -B, and -C antigens were screened by the conventional typing method, and then B22-group alleles including HLA-B54 were genotyped by single-strand conformation polymorphism analysis. Alleles of HLA-DRB1 gene were fully determined by the microtiter plate hybridization method. Thirty-seven percent of the patients possessed HLA-B*5401 allele conserved predominantly in East Asians, as compared with 15% of 110 healthy volunteers (chi2 = 12.4, p = 0.0004). In addition, 4% of the patients possessed B*5504 also unique to Asians but a rare allele which was not found in normal control subjects in this study. Typing of HLA-DRB1 class II gene did not demonstrate strong positive association with the disease. A33, B44, and DRB1*1302 showed negative association with the disease. We conclude that distinctive molecular structure of HLA-B alleles or a closely linked gene in the HLA region contributes to genetic predisposition in diffuse panbronchiolitis. This may partly explain why this disorder is found primarily in Asians. 相似文献
33.
Factors that affect the efficiency of in vitro synthesis of mutant proteins that contain nonnatural amino acids were investigated. The process of the nonnatural mutagenesis consists of chemical aminoacylation of a tRNA that contains a 4-base anticodon, followed by in vitro synthesis in the presence of an mRNA that contains the corresponding 4-base codon. Detailed studies on the time courses of the synthesis revealed two major factors that suppress the yield of nonnatural mutants compared with the wild-type protein. First, a cyclic tRNA that exists as a by-product of the chemical aminoacylation inhibits the protein synthesis. Second, the very short lifetime of a tRNA aminoacylated with a nonnatural amino acid limits the protein yield. As a simple and practical way of surmounting these factors, aminoacyl tRNA was added into the in vitro system at 5 min after the start of the synthesis. The addition increased the protein yield up to the level of conventional proteins in the in vitro system. 相似文献
34.
S. Ozaki Y. Nakata T. Nakamura S. Fukuyama Y. Ohkura 《Microelectronic Engineering》2010,87(3):370-372
We clarified that interfacial barrier metal oxidation with inter layer dielectric (ILD) could be revealed by X-ray photoelectron spectroscopy (XPS) on the peeled-side of the barrier metal, and the barrier metal oxidation was promoted by fluorine contamination which adsorb to the ILD surface during etching. To consider the effect of fluorine contamination on barrier metal oxidation, hydrolysable property of fluorine contamination was evaluated by measuring the change of F 1s spectrum after dipping in boiling water. Moreover, fluoride ions and the acidity of water in which fluorine contamination was dipped were measured by Ion chromatography and pH measurement, respectively. According to our experiments, it was suggested that hydrofluoric acid (HF) acted as an oxidizing catalyst to promote barrier metal oxidation at the interface of barrier metal and ILD. 相似文献
35.
Thixo-molded AE42 Mg alloy was friction stir welded, and the soundness of joints was evaluated, together with the microstructure evolution and mechanical properties in friction stir zones. According to X-ray radiography, the optimum FSW condition range of AE42 alloy exists between AZ61 and AZ31 alloys, and it seems that the optimum welding condition range increases with decreasing Al content in the Mg alloys. There are mainly two kinds of compounds in the thixo-molded AE42 alloy, and FSW has little influence on the grainy Al10RE2Mn7 compound, but it has great influence on Al11RE3 phase, which is changed from lamellar eutectic to small particles after welding. Furthermore, the average diameter of Al11RE3 particles in SZ decreases with increasing the traveling speed at constant rotation speed due to less heat input. The hardness in SZ is higher than that in BM, and tensile strength and elongation are both improved after welding because the stirring refines and uniforms the microstructure and intermetallic compounds. 相似文献
36.
Human calicivirus Sapporo (SV) has typical calicivirus morphology and causes acute gastroenteritis in children. The nucleotide sequence of 3.2 kb of the 3' end of SV was determined from a cloned cDNA. The 3' end of the SV genome is predicted to encode the RNA-dependent RNA polymerase region, the capsid protein and two small open reading frames. The nonstructural and capsid protein coding sequences in the SV genome are fused in a single open reading frame. The organization of these proteins in the SV sequence is similar to that of rabbit hemorrhagic disease virus and the recently described Manchester virus, and distinct from the genome organization of the prototype human calicivirus, Norwalk virus, that lacks typical calicivirus morphology and has been described as a small round structured virus (SRSV). Sequence analysis of the predicted capsid region showed that the SV capsid is longer by approximately 30 amino acids than the capsid of any of the SRSVs, and multiple sequence alignments showed that these additional amino acids are located in the variable region of the capsid protein. Expression of the capsid protein of SV in insect cells resulted in the self-assembly of virus-like particles that have a morphology similar to that of the native virus. This result shows that calicivirus morphology is determined by the primary sequence of the capsid protein. 相似文献
37.
Kosuke Nagaya Sadahiko Takeda Yoshitaka Nakata 《International journal for numerical methods in engineering》1986,23(6):1081-1099
This paper is concerned with the method for solving the vibration problem of coil springs of arbitrary shape. In the analysis the exact field transfer matrix is derived from the solution of the fundamental equation of the three-dimensional curved beam theory. The transfer matrix is given by combining this field transfer matrix and the point transfer matrix. The analysis derives the frequency equation which is applicable to coil springs of general shape. Numerical calculations have been carried out for hyperboloidal springs and barrel springs. Experimental tests also have been carried out to verify the present results. 相似文献
38.
39.
Yoshihiko Nakata Satoshi Ninomiya Chikage Imada Masafumi Nagai Takaaki Aoki Jiro Matsuo Nobutsugu Imanishi 《Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms》2005,230(1-4):489-494
We have measured both secondary neutral and ionized particles from an InSb target under 3.0 MeV Si ion bombardment. Measurements of both ions and neutrals have not been carried out so far in the MeV-energy range. The mass spectra and axial emission energy distributions of secondary particles were investigated. Secondary ions were measured with a linear- and a reflective-type time-of-flight technique, whereas secondary neutral particles were photo-ionized by a UV pulsed laser (ArF: 193 nm) and measured with a reflective-type time-of-flight technique. Different results were obtained for neutral particles in comparison with ionized particles. The mean energy of neutral Sb atoms was much lower than that of neutral In atoms, whereas the mean energies of secondary In and Sb ions were nearly equal. 相似文献
40.
Welding fume, inevitably generated during arc welding processes, may be a source of occupational pneumoconiosis when persistently inhaled in the long term and accordingly merits serious consideration in occupational hygiene respects. To improve the working environment at welding sites, it is imperative to ensure total air exchange at work sites, local extraction in the vicinity of welding facilities, and the wearing of respiration protection devices in other respects.1 Currently available preventive measures, however, suffer from a number of drawbacks, such as heavy investments needing to be made in equipment and facilities and operability being obstructed by the wearing of respiration protection devices. Alongside such passive measures, it is desirable to develop welding materials or methods able to suppress welding fume generation at source. 相似文献