Impact of N-Terminal Tags on De Novo Vimentin Intermediate Filament Assembly

Vimentin, a type III intermediate filament protein, is found in most cells along with microfilaments and microtubules. It has been shown that the head domain folds back to associate with the rod domain and this association is essential for filament assembly. The N-terminally tagged vimentin has been widely used to label the cytoskeleton in live cell imaging. Although there is previous evidence that EGFP tagged vimentin fails to form filaments but is able to integrate into a pre-existing network, no study has systematically investigated or established a molecular basis for this observation. To determine whether a tag would affect de novo filament assembly, we used vimentin fused at the N-terminus with two different sized tags, AcGFP (239 residues, 27 kDa) and 3 × FLAG (22 residues; 2.4 kDa) to assemble into filaments in two vimentin-deficient epithelial cells, MCF-7 and A431. We showed that regardless of tag size, N-terminally tagged vimentin aggregated into globules with a significant proportion co-aligning with β-catenin at cell–cell junctions. However, the tagged vimentin aggregates could form filaments upon adding untagged vimentin at a ratio of 1:1 or when introduced into cells containing pre-existing filaments. The resultant filament network containing a mixture of tagged and untagged vimentin was less stable compared to that formed by only untagged vimentin. The data suggest that placing a tag at the N-terminus may create steric hinderance in case of a large tag (AcGFP) or electrostatic repulsion in case of highly charged tag (3 × FLAG) perhaps inducing a conformational change, which deleteriously affects the association between head and rod domains. Taken together our results shows that a free N-terminus is essential for filament assembly as N-terminally tagged vimentin is not only incapable of forming filaments, but it also destabilises when integrated into a pre-existing network.     

Oxidative Stress and Chemoradiation-Induced Oral Mucositis: A Scoping Review of In Vitro,In Vivo and Clinical Studies

Chemoradiation-induced mucositis is a debilitating condition of the gastrointestinal tract eventuating from antineoplastic treatment. It is believed to occur primarily due to oxidative stress mechanisms, which generate Reactive Oxygen Species (ROS). The aim of this scoping review was to assess the role of oxidative stress in the development of Oral Mucositis (OM). Studies from the literature, published in MEDLINE and SCOPUS, that evaluated the oxidative stress pathways or antioxidant interventions for OM, were retrieved to elucidate the current understanding of their relationship. Studies failing inclusion criteria were excluded, and those suitable underwent data extraction, using a predefined data extraction table. Eighty-nine articles fulfilled criteria, and these were sub-stratified into models of study (in vitro, in vivo, or clinical) for evaluation. Thirty-five clinical studies evaluated antioxidant interventions on OM’s severity, duration, and pain, amongst other attributes. A number of clinical studies sought to elucidate the protective or therapeutic effects of compounds that had been pre-determined to have antioxidant properties, without directly assessing oxidative stress parameters (these were deemed “indirect evidence”). Forty-seven in vivo studies assessed the capacity of various compounds to prevent OM. Findings were mostly consistent, reporting reduced OM severity associated with a reduction in ROS, malondialdehyde (MDA), myeloperoxidase (MPO), but higher glutathione (GSH) and superoxide dismutase (SOD) activity or expression. Twenty-one in vitro studies assessed potential OM therapeutic interventions. The majority demonstrated successful a reduction in ROS, and in select studies, secondary molecules were assessed to identify the mechanism. In summary, this review highlighted numerous oxidative stress pathways involved in OM pathogenesis, which may inform the development of novel therapeutic targets.     

Association of Ligamentum Flavum Hypertrophy with Adolescent Idiopathic Scoliosis Progression—Comparative Microarray Gene Expression Analysis

The role of the ligamentum flavum (LF) in the pathogenesis of adolescent idiopathic scoliosis (AIS) is not well understood. Using magnetic resonance imaging (MRI), we investigated the degrees of LF hypertrophy in 18 patients without scoliosis and on the convex and concave sides of the apex of the curvature in 22 patients with AIS. Next, gene expression was compared among neutral vertebral LF and LF on the convex and concave sides of the apex of the curvature in patients with AIS. Histological and microarray analyses of the LF were compared among neutral vertebrae (control) and the LF on the apex of the curvatures. The mean area of LF in the without scoliosis, apical concave, and convex with scoliosis groups was 10.5, 13.5, and 20.3 mm², respectively. There were significant differences among the three groups (p < 0.05). Histological analysis showed that the ratio of fibers (Collagen/Elastic) was significantly increased on the convex side compared to the concave side (p < 0.05). Microarray analysis showed that ERC2 and MAFB showed significantly increased gene expression on the convex side compared with those of the concave side and the neutral vertebral LF cells. These genes were significantly associated with increased expression of collagen by LF cells (p < 0.05). LF hypertrophy was identified in scoliosis patients, and the convex side was significantly more hypertrophic than that of the concave side. ERC2 and MAFB genes were associated with LF hypertrophy in patients with AIS. These phenomena are likely to be associated with the progression of scoliosis.     

Diversity Analysis of Relay Selection Schemes for Two-Way Wireless Relay Networks

One-way wireless relay networks have recently received a lot of attention due to their ability to provide spatial diversity in fading wireless environment. Moreover, performing single-relay selection is a very attractive method due to its cost effective implementation and superior performance. However, one-way relay networks with the half-duplex signalling suffer from a spectral efficiency loss. To overcome such a drawback, two-way wireless relay networks have been proposed and these are also the networks considered in this paper. The paper analyzes the diversity orders of various relay selection schemes, including the best-relay selection, best-worse-channel selection, and maximum-harmonic-mean selection. The analysis is done for the amplify-and-forward protocol and under the two-step and three-step transmission procedures. In particular, it is shown that full diversity orders of R and R + 1 can be achieved in a R-relay wireless network with the two-step and three-step procedures, respectively. Numerical and simulation results are provided to verify our analysis.     

Review of The mind in context.

Reviews the book, The mind in context edited by Batja Mesquita, et al. (see record 2010-09987-000). The purpose of this edited volume is to challenge a predominant tendency toward an error of essentialism, or the attempt to explain psychological phenomena in terms of internal, static mental entities that are independent of and seemingly hermetically sealed from the outside world. Instead the introductory chapter of The mind in context advances the context principle: that mental processes and behaviour emerge from a profound and complex interaction between person and environment. It is important to note at the outset that a reader seeking an abstracted, sterilized, encyclopaedic list of essential elements making up this perspective would be well advised to look elsewhere. Rather, and in keeping with the theme of the book, the editors have assembled a lively collection of exemplars: chapters by prominent scholars each exploring the influence of context in a focused area of interest. The disadvantage of this approach is that the book can feel somewhat overextended. The significant advantage, however, is that the reader experiences each chapter in the context of the others, a juxtaposition that allows for the emergence of resonances, themes, and common principles across domains. In elaborating on the context principle the contributors to this book emphasise the mind’s mechanisms, situation-sensitivity, and agency over its content, permanence, and passivity. While written in large part by and for social psychologists, the volume should be of interest to any scholar fascinated by the mind’s ecological nature. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

A theory-based approach to teaching young children about health: A recipe for understanding.

The theory–theory account of conceptual development posits that children's concepts are integrated into theories. Concept-learning studies have documented the central role that theories play in children's learning of experimenter-defined categories but have yet to extensively examine complex, real-world concepts, such as health. The present study examined whether providing young children with coherent and causally related information in a theory-based lesson would facilitate their learning about the concept of health. This study used a pretest/lesson/posttest design, plus a 5-month follow-up. Children were randomly assigned to 1 of 3 conditions: theory (i.e., 20 children received a theory-based lesson), nontheory (i.e., 20 children received a nontheory-based lesson), and control (i.e., 20 children received no lesson). Overall, the results showed that children in the theory condition had a more accurate conception of health than children in the nontheory and control conditions, suggesting the importance of theories in children's learning of complex, real-world concepts. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Acculturation, enculturation, psychological distress and help-seeking preferences among Asian American college students.

We examined the relationship between Asian American college students' levels of acculturation, enculturation, and psychological distress. We also explored the methods Asian American college students prefer when seeking help for psychological concerns. The sample included 601 Asian American students from a large public university in Southern California. Respondents completed an online questionnaire, which included instruments assessing acculturation and enculturation levels as well as psychological distress and help-seeking preferences. Regression analyses indicated that when Asian American students hold a greater degree of European values they are less likely to experience psychological distress. A repeated-measures ANOVA found that Asian American students prefer more covert approaches to mental health treatment. These findings both compliment and contradict previous studies of acculturation, enculturation, psychological distress and help-seeking among the Asian American college student population. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Evaluation of Flow Resistance in Smooth Rectangular Open Channels with Modified Prandtl Friction Law

Flow resistance in open channels is usually estimated by applying the approach that is developed originally for pipe flows. Such estimates may be useful for engineering applications but always differ from measurements to some extent. This paper first summarizes empirical approaches that have been proposed in the literature to reconcile the resistance difference. These include various modifications of the pipe friction for applications to rectangular ducts and open channel flows. An improved friction equation is then derived for evaluating flow resistance of smooth rectangular open channels. Comparisons are made with experimental data reported by previous researchers and those collected in the present study. It is shown that the new proposed equation is applicable for both narrow and wide channels and is more accurate than those available in the literature.     

Stability of some stationary solutions to the forced KdV equation with one or two bumps

Free-surface flows past submerged obstacles in a channel are considered. The fluid is assumed to be inviscid and incompressible and the flow to be irrotational. The first-order approximation of long nonlinear surface waves over one or two bumps results in a forced Korteweg?Cde Vries (fKdV) equation. Solutions of the stationary fKdV equation are constructed and their stability is studied, either analytically or numerically. These various solutions include solitary waves over a single bump, solitary waves with two humps over a double bump, table-top solutions over a double bump and fronts.     

Synthesis of mesoporous tungsten oxide nanofibers using the electrospinning method

Mesoporous tungsten oxide nanofibers were synthesized via a 500 °C thermal treatment of composite nanofibers prepared by electrospinning an ethanol solution consisting of tungsten ethoxide, P123 triblock copolymer, and polyvinylpyrrolidone. The as-electrospun composites exhibited unwoven nanofibers with an average diameter of 233 nm and a smooth surface morphology. During the calcination process, the composite nanofibers were shrunk to 85 nm in diameter and converted into rough, wormhole-like nanofibers. These were formed by agglomerating polycrystalline WO₃ particles of 10–30 nm along the axial direction. Furthermore, a measured pore-size distribution indicated that this nanofiber mat had different types of meso-sized porosities, which may have resulted from their wormhole-like structures and inter-fiber voids. In addition, it was observed to have the intra-grain porosity with the diameter of about 1.0 nm.