HL-A antigens in serum from bone marrow harvested for diagnostic purposes

Tissue typing employing the lymphocytotoxicity micro-assay was performed by introducing in the system approximately 15% of autologous or homologous ultra-centrifuged blood serum, both of peripheral and bone marrow source. As a rule, the pattern of reactivity was unaffected by peripheral blood serum, whereas the cytotoxicity was nearly always inhibited by autologous bone marrow blood serum or by homologous one from donors sharing the inhibited HL-A specificities with the donors of lymphocytes. These results suggest that at least in these patients HL-A antigens are present in bone marrow blood serum at a concentration which is definitely higher than in peripheral blood.     

Numerical study of the interfaces of 3D‐printed concrete using discrete element method

3D concrete printing is an additive manufacturing method which reduces the time and improves the efficiency of the construction process. Structural behavior of printed elements is strongly influenced by the properties of the material and the interface surfaces. The printing process creates interface surfaces between layers in the horizontal and vertical directions. The bond strength between layers is the most critical property of printed elements. In this paper, the structural behavior of printed elements is studied using the discrete element method. The material is modelled using discrete particles with bonding between them. A new discrete model of a multilayer geometry is presented to study the behavior of the interfaces of printed concrete. The layers are made up of randomly placed particles to simulate the heterogeneous nature of concrete. The numerical model is developed to simulate the flexural behavior of multilayer specimens. A four‐point flexural test is simulated considering the interface surfaces between layers. This numerical model provides relevant results to improve the behavior of this kind of structural elements. The aim of this work is to provide a discrete element model to predict the mechanical behavior of 3D concrete printed components.     

Thin-Film Bulk Acoustic Wave Resonator Floating Above CMOS Substrate

A thin-film bulk acoustic wave resonator (FBAR) having a floating, 3-D structure above a CMOS substrate is presented. The integration of the FBAR to the CMOS substrate is performed with independence of FBAR or CMOS fabrication technologies. Wafer-level transfer is carried out to obtain a suspended FBAR above CMOS substrates of different technologies, whose resonant frequency is found in the 2.4 GHz band. The electrical interconnection between the FBAR and CMOS is provided by at least two conducting posts, which at the same time offer mechanical support to the resonator's structure. Experimental characterization results and Q-factor comparison with conventional FBAR technologies are discussed.     

Integrated CMOS amplifier for ENG signal recording

The development and in vivo test of a fully integrated differential CMOS amplifier, implemented with standard 0.7-microm CMOS technology (one poly, two metals, self aligned twin-well CMOS process) intended to record extracellular neural signals is described. In order to minimize the flicker noise generated by the CMOS circuitry, a chopper technique has been chosen. The fabricated amplifier has a gain of 74 dB, a bandwidth of 3 kHz, an input noise of 6.6 nV/(Hz)0.5, a power dissipation of 1.3 mW, and the active area is 2.7 mm2. An ac coupling has been used to adapt the electrode to the amplifier circuitry for the in vivo testing. Compound muscle action potentials, motor unit action potentials, and compound nerve action potentials have been recorded in acute experiments with rats, in order to validate the amplifier.     

Different Roles of Microalloying in Thin Slab Direct Rolling Technologies

HSLA steels constitute one of the main types of steels produced worldwide by Thin Slab Direct Rolling technologies (TSDR).Among microalloying elements,the most widely used are V,Nb and Ti.These elements play different roles during the austenite evolution in TSDR.Regarding austenite conditioning before transformation,the limitations in the total reduction that can be applied in TSDR technologies need to be considered when composition/process parameters are selected.In this context,whereas an important number of studies have been focused on Nb microalloyed grades,a less systematic analysis has been performed concerning the role of vanadium on austenite conditioning.This paper analyzes these singularities taking into account different process parameter conditions,such as total reduction and initial rolling temperature.     

Modulating Secondary Structure Motifs Through Photo-Labile Peptide Staples

Peptide-protein interactions (PPIs) are facilitated by the well-defined three-dimensional structure of bioactive peptides, interesting compounds for the development of new therapeutic agents. Their secondary structure and thus their propensity to engage in PPIs can be influenced by the introduction of peptide staples on the side chains. In particular, light-controlled staples based on azobenzene photoswitches and their structural influence on helical peptides have been studied extensively. In contrast, photolabile staples bearing photocages as a structural key motif, have mainly been used to block supramolecular interactions. Their influence on the secondary structure of the target peptide is under-investigated. Thus, in this study we use a combination of spectroscopic techniques and in silico simulations to systematically study a series of helical peptides with varying length of the photo-labile staple to obtain a detailed insight into the structure-property relationship in such photoresponsive biomolecules.