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11.
Continuous generation of hydrogel beads and encapsulation of biological materials using a microfluidic droplet-merging channel 总被引:1,自引:0,他引:1
In this paper, we describe a method for encapsulation of biomaterials in hydrogel beads using a microfluidic droplet-merging
channel. We devised a double T-junction in a microfluidic channel for alternate injection of aqueous fluids inside a droplet
unit carried within immiscible oil. With this device, hydrogel beads with diameter <100 μm are produced, and various solutions
containing cells, proteins and reagents for gelation could merge with the gel droplets with high efficiency in the broad range
of flow rates. Mixing of reagents and reactions inside the hydrogel beads are continuously observed in a microchannel through
a microscope. By enabling serial injection of each liquid with the dispersed gel droplets after they are produced from the
oil-focusing channel, the device simplifies the sample preparation process, and gel-bead fabrication can be coupled with further
assay continuously in a single channel. Instantaneous reactions of enzyme inside hydrogel and in-situ formation of cell-containing
beads with high viability are demonstrated in this report. 相似文献
12.
The effect of Pd on the growth rate of metal-induced lateral crystallization (MILC) from Ni seed and the electrical properties of thin-film transistors (TFTs) fabricated on the films crystallized by MILC were investigated. When the Pd metal is placed on the amorphous-silicon/Ni-seed layer, the MILC growth rate is two to three times faster than that of conventional Ni-MILC, without any degradation of TFTs. These results were explained by a stress that is generated by the formation of Pd2Si 相似文献
13.
Seong Sik Hwang Hong Pyo Kim Joung Soo Kim Kenneth E. Kasza Jangyul Park William J. Shack 《Nuclear Engineering and Design》2005,235(23):1060011-2484
A forced outage due to a steam generator tube leak in a Korean nuclear power plant has been reported [Kim, J.S., Hwang, S.S., et al., 1999. KAERI Internal Report (Korean). Destructive analysis on pulled tubes from Ulchin unit 1. Korea Atomic Energy Research Institute]. Primary water stress corrosion cracking has occurred in many tubes in the plant, and they were repaired using sleeves or plugs. In order to develop proper repair criteria, it is necessary to understand the leak behavior of the tubes containing stress corrosion cracks. Cracked specimens were prepared using a room temperature cracking technique, and the leak rates and burst pressures of the degraded tubes were determined both at room temperature and at a high temperature. Some tubes with 100% through wall cracks did not show a leakage at 10.8 MPa, which is the typical pressure difference of the pressurized water reactors (PWRs) during a normal operation. In some tests, the leak rates of the tubes increased with time at a constant pressure. In a high temperature pressure test at 282 °C one specimen showed a very small leakage at 18.6 MPa, which stopped after a small increase in the test pressure. Because stress corrosion cracks can develop at relatively low stresses, even 100% through wall cracks can be so tight that they will not leak at a normal operating pressure. 相似文献
14.
This paper addresses the problem of visual simultaneous localization and mapping (SLAM) in an unstructured seabed environment that can be applied to an unmanned underwater vehicle equipped with a single monocular camera as the main measurement sensor. Monocular vision is regarded as an efficient sensing option in the context of SLAM, however it poses a variety of challenges when the relative motion is determined by matching a pair of images in the case of in-water visual SLAM. Among the various challenges, this research focuses on the problem of loop-closure which is one of the most important issues in SLAM. This study proposes a robust loop-closure algorithm in order to improve operational performance in terms of both navigation and mapping by efficiently reconstructing image matching constraints. To demonstrate and evaluate the effectiveness of the proposed loop-closure method, experimental datasets obtained in underwater environments are used, and the validity of the algorithm is confirmed by a series of comparative results. 相似文献
15.
Moumita Mukherjee Biswanath Mukherjee Youngill Choi Kyoseung Sim Junghwan Do Seungmoon Pyo 《Synthetic Metals》2010,160(5-6):504-509
We report a copper hexadecafluorophthalocyanine (F16CuPc) based n-type organic field-effect transistor (OFET) with polymeric gate dielectrics with different physical/electrical properties. The gate dielectrics are four types of cross-linked poly(4-vinylphenol) and newly prepared poly(4-phenoxy methyl styrene) and those are characterized based on surface tension, leakage current and capacitance. The performance of F16CuPc OFETs with those gate dielectrics was compared. We found that the composition of the gate dielectrics and the interfacial interaction of F16CuPc with the gate dielectric play a decisive role in the performance of OFETs. The effect of physical/electrical properties, composition and processing condition of the gate dielectrics on the device performance was investigated. 相似文献
16.
Kee Do Woo Jae Hwang Kim Eui Pyo Kwon Min Seok Moon Hyun Bom Lee Tatsuo Sato Zhiguang Liu 《Metals and Materials International》2010,16(2):213-218
To fabricate an Al-V matrix composite reinforced with submicron-sized Al2O3 and AlxVy (Al3V, Al10V) phases, high energy mechanical milling (HEMM) and sintering were employed. By increasing the milling time, the size of
mechanically milled powder was significantly reduced. In this study, the average powder size of 59 μm for Al, and 178 μm for
V2O5 decreased with the formation of a new product, Al-Al2O3-AlxVy, with a size range from 1.3 μm to 2.6 μm formed by the in-situ combustion reaction during sintering of HEM milled Al and
V2O5 composite powders. The in-situ reaction between Al and V2O5 during the HEMM and sintering transformed the Al2O3 and AlxVy (Al3V, Al10V) phases. Most of the reduced V reacted with excess the Al to form AlxVy (Al3V, Al10V) with very little V dissolved into Al matrix. By increasing the milling time and weight percentage of V2O5, the hardness of the Al-Al2O3-AlxVy composite sintered at 1173 K increased. The composite fabricated with the HEMM Al-20wt.%V2O5 composite powder and sintering at 1173 K for 2 h had the highest hardness. 相似文献
17.
Autonomous agents traversing a natural space need to be knowledgeable of its space configuration. The existing space models in geographic information systems and robotics, however, deal with only the topological relations among numerous types of spatial relations. We aim to develop an enhanced space model that elaborates the spatial relations with respect to their relevant physical relations. Specifically, the spatial relations in a space configuration are further characterized with the gravitation as a potential factor to affect the space configuration. The resulting space model is capable of capturing an extended set of spatial relations over existing models such as a four‐intersection model. © 2005 Wiley Periodicals, Inc. Int J Int Syst 20: 867–892, 2005. 相似文献
18.
Dae‐Sik Lee Hyoung Gil Choi Kwang Hyo Chung Bun Yeoul Lee Hyeon‐Bong Pyo Hyun C. Yoon 《ETRI Journal》2007,29(5):667-669
This letter presents a smart integrated microfluidic device which can be applied to actively immobilize proteins on demand. The active component in the device is a temperature‐controllable microelectrode array with a smart polymer film, poly(N‐isopropylacrylamide) (PNIPAAm) which can be thermally switched between hydrophilic and hydrophobic states. It is integrated into a micro hot diaphragm having an integrated micro heater and temperature sensors on a 2‐micrometer‐thick silicon oxide/silicon nitride/silicon oxide (O/N/O) template. Only 36 mW is required to heat the large template area of 2 mm×16 mm to 40°C within 1 second. To relay the stimulus‐response activity to the microelectrode surface, the interface is modified with a smart polymer. For a model biomolecular affinity test, an anti‐6‐(2, 4‐dinitrophenyl) aminohexanoic acid (DNP) antibody protein immobilization on the microelectrodes is demonstrated by fluorescence patterns. 相似文献
19.
20.
A new chelating fabrics are synthesized by radiation induced grafting of acrylonitrile (AN) onto polypropylene nonwoven fabrics (PP fabrics) and subsequent conversion of cyano to an imine by reaction with ethylene diimine (EDA). Influence of various parameters such as initial concentration, pH and adsorption time on the adsorption amount for palladium ions was investigated. It was found that adsorbent achieved excellent adsorption performance in palladium ion removal in the pH range of 1.88–5.70. The adsorption amount of ions increased with shaking time and initial concentration of palladium ions. The grafted and aminated fabrics were characterized by FT‐IR, TGA and SEM. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010 相似文献