Acetyl‐triacylglycerols (acetyl‐TAG) possess an sn‐3 acetate group, which confers useful chemical and physical properties to these unusual triacylglycerols (TAG). Current methods for quantification of acetyl‐TAG are time consuming and do not provide any information on the molecular species profile. Electrospray ionization mass spectrometry (ESI–MS)‐based methods can overcome these drawbacks. However, the ESI–MS signal intensity for TAG depends on the aliphatic chain length and unsaturation index of the molecule. Therefore response factors for different molecular species need to be determined before any quantification. The effects of the chain length and the number of double‐bonds of the sn‐1/2 acyl groups on the signal intensity for the neutral loss of short chain length sn‐3 groups were quantified using a series of synthesized sn‐3 specific structured TAG. The signal intensity for the neutral loss of the sn‐3 acyl group was found to negatively correlated with the aliphatic chain length and unsaturation index of the sn‐1/2 acyl groups. The signal intensity of the neutral loss of the sn‐3 acyl group was also negatively correlated with the size of that chain. Further, the position of the group undergoing neutral loss was also important, with the signal from an sn‐2 acyl group much lower than that from one located at sn‐3. Response factors obtained from these analyses were used to develop a method for the absolute quantification of acetyl‐TAG. The increased sensitivity of this ESI–MS‐based approach allowed successful quantification of acetyl‐TAG in various biological settings, including the products of in vitro enzyme activity assays. 相似文献
In recent years, researchers have developed biosurfactants for industrial, pharmaceutical and medical applications revealing the promising biological activities of these biomolecules. One of the best studied microbial surfactants are glycolipids, especially sophorolipids (SLs) produced by selected non-pathogenic yeast species of Candida. They are biodegradable, non-toxic and are environmentally friendly. Sophorolipid production was carried out using glucose as the hydrophilic source and lauryl alcohol C12–14, as the hydrophobic source using Candida bombicola ATCC 22214. Primary characterization of the SL obtained using lauryl alcohol (SLLA) was done by FTIR which depicted the presence of alkyl sophorosides/SLs. Antimicrobial activity testing revealed that SLLA showed complete inhibition against gram negative bacteria, Escherichia coli (ATCC 8739) Pseudomonas aeruginosa (ATCC 9027) at 30 and 1 μg/ml at a contact time of 2 and 4 h respectively. Whereas for gram positive bacteria Staphylococcus aureus (ATCC 6358), Bacillus subtilis (ATCC 6633), complete inhibition was observed at 6 and 1 μg/ml respectively at a contact time of 4 h. The formed SLLA showed noteworthy inhibition against the pathogenic yeast Candida albicans (ATCC 2091) at 50 μg/ml with a contact time of 4 h. These values are remarkably low compared to reported values of oleic acid SLs and linolenic acid SLs which were studied for antimicrobial properties. Scanning electron microscopy analysis of the treated cells revealed the changes in morphology and topography of the microorganisms. 相似文献
A multiset is a collection of objects in which repetition of elements is significant. In this paper an attempt is made to generalize the concepts of relation, function, composition and equivalence in the multiset context. As a pre-requisite a brief survey of the axiomatic approach to the multiset theory is also presented. 相似文献
In accordance with the FAA certification requirements, all modern commercial turbofan engines must successfully demonstrate its ability to withstand a fan blade-out (FBO) event through actual test. Possibility of losing a rotating fan blade from a running engine is a flight safety consideration, which must be addressed during the design phase of the engine. A typical fan blade-out event involves very complex nonlinear transient dynamics with large deflection of the release blade and rigid body rotation of the trailing blade as well as progressive failure and fragmentation of various components. Due to the nature of the impact type loading, the solution to the problem should also address dependence of the material behavior such as yield strength as a function of strain rates. In short, the transient dynamic analysis of a fan blade-out event highlights the complexity of the numerical technique, which includes all the nonlinearities of structural dynamics: plastic behavior of the materials, large displacements, and contact interaction between structural elements. In this paper, we present the results of a LS-DYNA simulation of a FBO event on a full-engine analytical model, which covers both the primary as well as secondary damages. 相似文献
A novel class of organic‐inorganic polymer hybrids was developed by melt‐blending up to 50 (v/v) % [about 83 (w/w) %] tin‐based polyphosphate glass (Pglass) and low‐density polyethylene (LDPE) in conventional plastics processing equipment. The liquid‐ and solid‐state rheology of the polymer hybrids was studied under oscillatory shear flow and deformation to understand the behavior of these materials and to accelerate efforts to melt process the Pglass with organic polymers. All the materials were found to be linearly viscoelastic in the range of temperature and frequencies examined and their viscoelastic functions increased with increasing Pglass concentration. The Pglass significantly enhanced the shear‐thinning characteristics of the Pglass‐LDPE hybrid, indicating the presence of nonlinear chemical and physical interactions between the hybrid components. Morphological examination of the materials by scanning electron microscopy revealed interesting evolution of microstructure of the Pglass phase from droplets (or round beads) to elongated and interpenetrating network structures as the glass concentration was increased in the Pglass‐LDPE hybrids. Melt viscosities of the materials were well described by a simple power‐law equation and a Maxwellian (Hookean) model with three relaxation times. Time‐temperature superpositioning (TTS) of the complex viscosity versus frequency data was excellent at 170°C < T < 220°C and the temperature dependencies of the shift factors conformed excellently well to predictions from an Arrhenius‐type relation, enabling calculation of the flow‐activation energies (25–285 kj/mol) for the materials. The beneficial function of the Pglass in the hybrid system was significantly enhanced by pre‐treating the glass with coupling agents prior to incorporating them into the Pglass‐LDPE hybrids. 相似文献
Extensive experimental tests and a computational study of the performance in a cross-flow air classifier have been carried out. A computational fluid dynamics (CFD) package—Fluent—is used to first understand and explain why the cuts or the sharpness of cut of this classifier are not as sharp as they ought to be, and then to optimize the geometry and operational conditions.
Flow fields of the classifier under various set-up conditions and geometry were measured by using laser Doppler anemometry (LDA). Using sieve analyses and the HELOS-laser method, the patterns of behaviour of separation parameters such as cut size and sharpness of cut have been investigated at different boundary conditions.
Using the Fluent package, a two-dimensional computational fluid dynamics model has been developed. The model is based on the Euler–Lagrangian approach. Different turbulence models have been tested. Both Fluent 4.5, with a structured grid, and Fluent 5.1, with structured and unstructured grids, have been used.
Discussions and analyses of the experimental, as well as the computational results, are presented. The simulation with a structured grid shows good agreement with experimental data, except for the sharpness of cut. The reasons of poor performance of the classifier have been found. The geometry is optimized and other conditions were also improved. The performance of the classifier is improved. The experimental observations together with the computed results should increase the depth of understanding of the underlying mechanisms. 相似文献