Isolation of Decidual Macrophages and Hofbauer Cells from Term Placenta—Comparison of the Expression of CD163 and CD80

(1) Background: Placental immune cells are playing a very important role in a successful placentation and the prevention of pregnancy complications. Macrophages dominate in number and relevance in the maternal and the fetal part of the placenta. The evidence on the polarization state of fetal and maternal macrophages involved in both, healthy and pregnancy-associated diseases, is limited. There is no representative isolation method for the direct comparison of maternal and fetal macrophages so far. (2) Material and Methods: For the isolation of decidual macrophages and Hofbauer cells from term placenta, fresh tissue was mechanically dissected and digested with trypsin and collagenase A. Afterwards cell enrichment was increased by a Percoll gradient. CD68 is represented as pan-macrophage marker, the surface markers CD80 and CD163 were further investigated. (3) Results: The established method revealed a high cell yield and purity of the isolated macrophages and enabled the comparison between decidual macrophages and Hofbauer cells. No significant difference was observed in the percentage of single CD163⁺ cells in the distinct macrophage populations, by using FACS and immunofluorescence staining. A slight increase of CD80⁺ cells could be found in the decidual macrophages. Considering the percentage of CD80⁺CD163⁻ and CD80⁻CD163⁺ cells we could not find differences. Interestingly we found an increased number of double positive cells (CD80⁺CD163⁺) in the decidual macrophage population in comparison to Hofbauer cells. (4) Conclusion: In this study we demonstrate that our established isolation method enables the investigation of decidual macrophages and Hofbauer cells in the placenta. It represents a promising method for direct cell comparison, enzyme independently, and unaffected by magnetic beads, to understand the functional subsets of placental macrophages and to identify therapeutic targets of pregnancy associated diseases.     

Obstacles for cognitive analogy in analysis and synthesis in microsystems development

In most cases, product development is not inventing a product completely new and from scratch, it typically either adds a few newly developed sub-functions to mainly known sub-functions, or it generates invention based on new combinations of already existing and slightly modified sub-functions. This synthesis is ideally based on a preceding and comprehensive system analysis that is necessary to define promising directions for advancement in further product development. In analysis as well as in synthesis the design engineer needs creativity—either to be able to recognize which functions are realized in a given system that is subject to analysis or to be able to think up new ways how a desired function could be realized in a system. The main cognitive mechanism for creativity is analogy and this mechanism in turn is based on schemas and mental models. In microsystems development, micro specific obstacles accrue when using analogies. In this work, the authors derive the nature and source of these obstacles and their implications on microsystems development. To overcome these obstacles, a functions catalogue is proposed. This catalogue can directly be used to add an external source for analogies during system synthesis. By transferring a method called geometrical similarity search to microsystems technology and combining it with the functions catalogue, system analysis can be supported as well.     

Influence of Whey Protein Addition and Feed Moisture Content on Chosen Physicochemical Properties of Directly Expanded Corn Extrudates

Production of extrudates from cereals is an often-used technological process in today’s world food industry. Extrudates from corn flour produced using the twin-screw extrusion process and enriched with whey protein concentrate represent high-quality source of proteins and fats. Whey protein concentrate (WPC) as a valuable source of proteins and minerals is one of the highest-quality components for possible extrudate enrichment. In this paper, the influence of various WPC addition and some extrusion process parameters such as feed moisture content ( Q_{textHtext2 textO} Q_{{{text{H}}_{text{2}} {text{O}}}} ) on physicochemical properties of directly expanded corn flour extrudates manufactured in twin-screw co-rotating extruder was investigated. Whey protein concentrate was added in the following ratios 7.5%, 15% and 22.5% and water in 10.08, 12.18 and 14.28 L/h. Final composition of products is determined with measuring of protein, fat and water shares, water absorption index (WAI) and water solubility index (WSI). With added WPC and with increase of water volume flow, there was a significant rise in total protein, fat and water content in final products, as well as lowering of WSI and rising of WAI indexes. The statistical analysis of the obtained data shows that the lowest WSI and the highest WAI had samples with the largest share of WPC (22.5%) and water volume flow of 14.28 L/h. Colour is measured for each sample, and results were represented with hue angle (H), chroma (C) and lightness (L) values. Process parameters, WPC and Q_{textHtext2 textO} Q_{{{text{H}}_{text{2}} {text{O}}}} influence the increase of saturation of C and lightness of L colour value, while H value stays unchanged. Mean value of H was 90.14 ± 1.06, which corresponds to dominance of yellow colour of samples.     

Minimum cost flows with minimum quantities

We consider a variant of the well-known minimum cost flow problem where the flow on each arc in the network is restricted to be either zero or above a given lower bound. The problem was recently shown to be weakly NP-complete even on series-parallel graphs. We start by showing that the problem is strongly NP-complete and cannot be approximated in polynomial time (unless P=NP) up to any polynomially computable function even when the graph is bipartite and the given instance is guaranteed to admit a feasible solution. Moreover, we present a pseudo-polynomial-time exact algorithm and a fully polynomial-time approximation scheme (FPTAS) for the problem on series-parallel graphs.     

Experimental Investigation of the Flow in a Continuous-Casting Mold under the Influence of a Transverse,Direct Current Magnetic Field

This article describes laboratory experiments aimed at investigations of flow structures and related transport processes in the continuous-casting mold under the influence of an external direct current (DC) magnetic field. The main value of cold metal laboratory experiments consists in the capabilities to obtain quantitative flow measurements with a reasonable spatial and temporal resolution. The experimental results presented here were obtained using a physical model operating with the room-temperature alloy GaInSn. According to the concept of the electromagnetic brake, the impact of a DC magnetic field on the outlet flow from the submerged entry nozzle (SEN) has been studied up to Hartmann numbers of approximately 400. The effect of the magnetic field on the flow structure turned out to be complex. The flow measurements do not manifest a general braking effect, which would be expected as an overall damping of the flow velocity and the related fluctuations all over the mold volume. Variations of the wall conductivity showed a striking impact on the resulting flow structures. The experiments provide a substantial database for the validation of respective numerical simulations.     

An Innovative Submerged Entry Nozzle Design for Billet and Bloom Casting

Fluid flow in the mold has a significant impact on the quality of continuously cast steel products.Since it is strongly influenced by the SEN design,attention h...     

Photoinitiierte Polymerisation mit Dialkoxythiocarbonyldisulfiden – eine Polymerisationsreaktion mit Primärradikalabbruch

Photoinitiated Polymerization with Dialkoxy Thiocarbonyl Disulfides – a Polymerization Reaction with Primary Radical Termination The photoinduced free radical polymerization of methyl methacrylate (MMA) and some other vinyl monomers using dialkoxy thiocarbonyl disulfides ( 1 – 4 ) as photoinitiators was studied. The photolysis of these initiators leads to cleavage of the S S bond, which was determined by spin trapping experiments with phenyl tert-butyl nitrone. No evidence was found for a further thermal decomposition of the primary alkoxy thiocarbonyl sulfide radicals (R·) at room temperature. In the absence of scavengers the primary radicals react back exclusively to the initial compounds, due to a strong cage effect. By means of UV spectroscopic measurements and in the presence of MMA the quantum yields of the initiator decomposition were detected to be 0.8. The polymerization of acrylic and methacrylic derivatives can be initiated by the R·, contrary to the situation with fumarates and maleates. The mechanism of MMA polymerization depends on the light intensity absorbed by the initiators. At high intensities the combination of primary and polymer radicals terminates the chain reaction. This follows from the measured monomer exponent of α = 2, the light intensity exponent of β = 0,2 and the number of thiocarbonyl end groups of 2 in the polymers isolated. Contrary to this, in the low intensity region the experimental data obey the ideal kinetic equation. Kinetic modelling gives evidence for an interaction of the monomer with the cage radicals.