Studies of substrate specificities of lipases from different sources

Although lipases are widely applied in a wide variety of reactions, available information on the factors that are responsible for the substrate specificities of lipases from different sources is scarce. In this paper, nine lipase‐producing microorganism strains were isolated from oil‐containing samples. The properties of these enzymes, including pH optima, temperature optima, thermal stability, and pH stability, vary significantly with the different sources from which these lipases were isolated. The substrate specificities of the nine lipases, including fatty acid and positional specificities, were also studied. The fatty acid specificities of the nine lipases were observably different toward 15 substrates with different carbon chain lengths, different saturation degrees and different side chains. The lipases from S₃ Penicillium citrinum (PCL), MJ₁ Aspergillus niger (ANL), MJ₂ Aspergillus oryzae (AOL), YM Bacillus coughing (BCL), S₉ Geotrichum candidum (GCL), and S₁₁ Candida lypolytica (CLL) showed the strongest specificities to short‐chain esters, and the other lipases showed strong selectivity for medium‐ or long‐chain and branched esters. The positional specificities were examined by analyzing the hydrolytic products of triolein catalyzed by the nine lipases, using TLC. The lipases can be mainly classified into two groups by their specificities for the ester bond at position 2 of triglycerides; one group can selectively hydrolyze the ester bond at position 2 of the triglycerides, the other group cannot. All these nine lipases were divided into four groups by hierarchical clustering analysis on the basis of the results of fatty acid and positional specificities.     

An online technique for monitoring the insulation condition of AC machine stator windings

A novel online technique for monitoring the insulation condition of ac machine stator windings is proposed in this paper. The concept is to measure the differential leakage currents of each phase winding from the terminal box in a noninvasive manner to assess the insulation condition during motor operation. The conventional differential CTs used for phase fault protection can be replaced with high performance current sensors to measure the leakage current with higher accuracy. Indicators for insulation condition such as the capacitance and dissipation factor are calculated based on the measurements to provide a low cost solution for online insulation condition assessment. A simplified online insulation system model is derived for analysis and interpretation of the measured data. Experimental results on a 15-hp induction motor under simulated insulation degradation conditions show that the proposed technique is a very sensitive method capable of detecting incipient signs of insulation degradation.     

钢筋混凝土非正常裂缝的产生原因及应对措施

根据钢筋混凝土结构中非正常裂缝的种类，分析了各种裂缝的产生原因，并有针对性地提出了应对措施，以保证建筑物和构件安全和稳定地工作。     

核磁共振测井在桩1区块底水稠油油藏开发中的应用

针对桩1区块特高含水期底水稠油油藏的开发特点,利用核磁共振测井资料定量评价其孔隙流体流动特性,准确划分产层,直接识别油、水层,准确地评价了区块剩余油分布情况.依此部署了水平井,取得了很好的开发效果.实践证明,核磁共振测井具备常规测井所不具备的某些技术优势,能解决一些特殊的地质问题,有广泛的应用前景.     

短脉冲激光与岩石相互作用机理初探

石油开采过程中，为增大原始岩体的渗流速度，一般采用水压致裂或射孔弹的方法来加以实现，但两种方法都存在一定缺陷。为探索一种新概念石油开采技术，即采用激光技术增大原始岩体的渗透率，对短脉冲激光辐照自然、饱水和饱油状态的花岗岩、大理岩和红砂岩的响应作了初步研究。得到了激光辐照下，岩石试样内烧蚀坑的三维形态以及烧蚀状态与脉冲数的初步关系。结果表明：脉冲激光可以在试样局部产生一定的裂纹；饱油试样的烧蚀情况较饱水和自然状态更严重。但对于产生的裂纹数量，尺寸以及方向等方面的控制尚需进一步的工作。     

龙滩水电站尾水调压井桥机拆除

龙滩水电站尾水调压井施工桥机在完成其施工吊装使命后需要拆除，为保证其完好率，需采取无破坏法整机拆除。介绍了在狭小空间大型设备的整机拆除方法和天锚系统的设计、布局。     

基于ANSYS的铸嘴流体耦合场三维有限元仿真分析

作者用大型有限元分析软件ANSYS对铸嘴内稳态层流铝熔体进行仿真分析。实现了三维建模。解决了三维热一流耦合求解的问题，得到铝流体的速度、压力及温度的分布图。分析了铸嘴出口速度和温度的分布规律及其对连续铸轧的影响。     

高厚径比盲孔电镀

盲孔能够大大的增加板的密度,从而减小板面积。介绍了有关高厚径比盲孔电镀的有关工艺,从激光钻孔、金属化孔和电镀几个方面对孔径4mil以上的盲孔电镀进行了研究。     

波浪力计算中的一个新边界元方法

本文就波浪与结构物作用问题，提出了一个新的高阶边界元方法。较之Ｅａｔｏｃｋ Ｔｏｙｌｏｒ和Ｃｈａｕ的方法，本文在体内附助积分方程中应用了满足刚性水面条件和弱刚性底面条件的简单格林函数。计算速度相应地有所提高；漂浮结构的积分方程更为简洁；计算结果与Ｅａｔｏｃｋ Ｔａｙｌｏｒ和Ｃｈａｕ的方法具有几乎相同的精度和收敛速度。