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71.
A pneumatically actuated silicon microvalve applicable to integrated microfluidic systems is presented. All the ports of this microvalve are in-channel, and connectable to any surface fluid channels in microfluidic systems. This microvalve controls fluid flow by means of the controlled gap between glass and silicon diaphragm actuated by a control pressure. In addition, the diaphragm is also deformed by the outlet pressure of the microvalve. Due to the feature, this microvalve shows saturation of flow rate like MOSFETs operated at saturation region. The fabricated microvalve device was evaluated focusing on analogous relationship between MOSFET and the microvalve. Fluids such as air and DI-water were well controlled by the control pressure. Fluid starts to flow in the microvalve when the control pressure exceeds its "threshold pressure." Hysteresis due to sticking of diaphragm was not observed in the characteristics. Air flow rate of the microvalve was gradually saturated with the increasing of the outlet pressure as expected. Through the evaluation, analogous relationship between this microvalve and MOSFET has been experimentally demonstrated. 相似文献
72.
A novel osmium-metal coating device for SEM observation has been developed to prevent negative charge build-up on specimens by applying the hollow-cathode low voltage discharge plasma chemical vapour deposition (CVD) method. The CVD method using the hollow-cathode offers the following advantages. (i) The method can deposit osmium-metal at very low discharge voltage that is as low as half of that of the planar parallel electrode method. Therefore, the method avoids damage due to ion bombardment during the coating process. (ii) The method can minimize the quantity of the OsO4 gas by introducing directly into the hollow-cathode. This feature is important to prevent the air pollution caused by the purged gas. (iii) A large coating area is guaranteed because the Os ion is filled in the hollow-cathode where the specimen is holed. (iv) The lower discharge voltage can be used by mixing Ar, N2 or air with the OsO4 gas as the environmental gas in the chamber. (v) The hybrid coating is also available by lining the appropriate metal material such as platinum (Pt) on the surface of the inside of the hollow-cathode. The method uses the plasma CVD of Os metal as well as the ion-sputter deposition of the lined metal. 相似文献
73.
74.
Y Matsushita K Yoshiie Y Imamura H Ogawa H Imamura S Takao S Yonezawa T Aikou I Maruyama E Sato 《Canadian Metallurgical Quarterly》1998,127(1-2):195-201
Inside-out vesicles of plasma membranes prepared from a plant source were used as models to investigate effects of centrifugal forces on separations of early and late endosome populations by aqueous two-phase partition. Endosome subpopulations were resolved readily by preparative free-flow electrophoresis where acidification of the interiors of late endosomes occurred upon addition of ATP to activate a proton translocating ATPase. The resultant increased diffusion potential provided for a surface difference between late and early endosomes to permit electrophoretic separation. With the plant membranes, unincubated inside-out plasma membrane vesicles modeled early endosomes, whereas inside-out vesicles incubated with 1 mM ATP modeled late endosomes. A latent, 2,4-dichlorophenoxyacetic acid (2,4-D)-(auxin)-stimulated NADH:protein disulfide reductase measured spectrophotometrically was used as an enzymatic marker for both populations of inside-out vesicles. Phase partition behavior of each population was quantitated using total protein as the parameter. 相似文献
75.
T Sakuma K Takahashi N Ohya K Usuda M Handa T Abe 《Canadian Metallurgical Quarterly》1998,353(2-3):273-279
The imbalance of neutrophil elastase and alpha1-antitrypsin in pleural effusion after lobectomy and the effects of the neutrophil elastase inhibitors, sodium N-[2-[4-(2,2-Dimethylpropionyloxy)phenyl-sulfonylamino]benzo yl]aminoacetic acid (ONO-5046) and purified alpha1-antitrypsin, on neutrophil elastase activity were determined. The amount of neutrophil elastase complexed to alpha1-antitrypsin, measured by an enzyme-linked immunosorbent assay, was 170 times higher in pleural effusion than in blood 3 h after lobectomy. The alpha1-antitrypsin levels measured by laser nephelometry did not increase in either blood or pleural effusion. Although neutrophil elastase activity, measured by the hydrolysis of succinyl-(Ala)3-p-nitroanilide, was not detected in blood, it was increased in pleural effusion 3 h and 24 h after lobectomy. ONO-5046, but not alpha1-antitrypsin, reduced the neutrophil elastase activity in pleural effusion. There is an imbalance of neutrophil elastase and alpha1-antitrypsin in pleural effusion after lobectomy. ONO-5046 is a potent inhibitor of neutrophil elastase activity in human pleural effusion. 相似文献
76.
T Matsuda Y Hashimoto H Ueda T Asano Y Matsuura T Doi T Takao Y Shimonishi Y Fukada 《Canadian Metallurgical Quarterly》1998,37(27):9843-9850
Among 11 subtypes of heterotrimeric G-protein gamma-subunit, gamma1 (rod), gamma8 (cone) and gamma11 are modified with farnesyl while the others are modified with geranylgeranyl at the C-terminus. To understand the role of specific isoprenylation (farnesylation) of retinal transducin, we examined how and to what extent the type of isoprenyl group affects transducin-beta gamma (beta1 gamma1) functions such as interactions with membranes, Galpha/receptor, and effectors. To this end, the C-terminal farnesylation signal sequence (CVIS) of gamma1 was replaced by a geranylgeranylation signal (CVIL), and the resultant mutant (S74L) or wild-type (WT) gamma1 was coexpressed with beta1 in the baculovirus-Tn5 insect cell system. Both gamma1WT and gamma1S74L expressed as a beta gamma complex were mixtures modified with farnesyl and geranylgeranyl groups. The ratio of farnesyl to geranylgeranyl in preparations of beta1 gamma1WT and beta1 gamma1S74L purified from the Tn5 cell membrane fraction was about 1:2 and 1:6, respectively. These two forms of recombinant beta1 gamma1 and retinal beta1 gamma1 were different in their abilities to associate with rod outer segment membranes with the following rank order: beta1 gamma1S74L > beta1 gamma1WT > retinal beta1 gamma1. Functionally, beta1 gamma1S74L was the most potent to promote pertussis toxin-catalyzed ADP ribosylation of transducin-alpha (Talpha), to stimulate metarhodopsin II-catalyzed GTPgammaS-binding reaction to Talpha and to modulate adenylyl cyclase and phospholipase C activities. All of the beta1 gamma1 functions absolutely required the isoprenylation of the gamma-subunit. As for the interaction with Goalpha and adenylyl cyclase, predominantly geranylgeranylated beta1 gamma1S74L was less effective than geranylgeranylated beta1 gamma2 purified from bovine brain. These results demonstrate that the properties of Gbeta gamma are strongly affected by the type of functionally indispensable isoprenylation in addition to the amino acid sequence of Ggamma. The relative contribution of the two factors depends on proteins with which Gbeta gamma interacts. 相似文献
77.
78.
Koshiro Hashimoto Takao Fujisawa Masahiro Kobayashi Ryutoku Yosomiya 《应用聚合物科学杂志》1982,27(12):4529-4539
Modified glass fibers, containing unsaturated hydrocarbon surface groups, were prepared by a hydrothermal treatment, with allylglycidylether in excess as reagent. Graft polymerization of the treated glass fiber with styrene and methylmethacrylate was carried out in sealed tubes, under nitrogen, using benzoyl peroxide (BPO) and cumene hydroperoxide (CHPO) as initiators. When BPO was used as the initiator, the grafting efficiency was extremely low, but the graft copolymerization behavior was similar to that of usual organic polymers. With CHPO, both grafting ratio and grafting efficiency were very high. Various properties of composite materials containing grafted glass cloth were studied. Flexural strength, flexural modulus, and interlaminar shear strength increased proportionally to the increase of the grafting ratio; the same values were decreased only in a small extent after the boiling test. 相似文献
79.
Katunori Abe Takao Takeuchi Michio Kikuchi Shotaro Morozumi 《Journal of Nuclear Materials》1981,99(1):25-37
Molybdenum specimens prepared by two processes, powder-metallurgy (PM) and electron-beam melting (EB), were irradiated to a fast neutron fluence of 2.74 × 1024n/m2 (En? 1 MeV) at about 600°C (873 K), and their mechanical properties were studied in detail. It was shown that the degree of irradiation embrittlement in EB-Mo was smaller than that in PM-Mo, which might be caused by stronger grain-boundaries and probably smaller irradiation-hardening in the former. From the relation between the recovery of ductility and microstructural changes in post-irradiation annealed PM-Mo at 800 (1073 K), 1000 (1273 K) and 1200°C (1473 K), it was concluded that the recovery resulted from a decrease of irradiation hardening due to a rearrangement and a disappearance of depleted-zones, dislocation-loops and voids in order with increasing annealing temperature. An anomalous mode of fracture was observed in as-irradiated specimens, which consisted of inhomogeneous deformation, then brittle fracture not at the center but at the root of the deformation neck. This mode was observed in a narrow temperature range near the DBTT. A possible mechanism is discussed. 相似文献
80.