Stable expression of a thermostable xylanase of Clostridium thermocellum in cultured tobacco cells

Two distinct domains of the xynA gene from Clostridium thermocellum encoding a xylanase catalytic domain (XynAl) and a xylanase catalytic domain with a cellulose binding domain (XynA2) under the control of the cauliflower mosaic virus 35S promoter were electroporated into cultured tobacco BY-2 cells. Transgenic BY -2 calli expressing xylan-hydrolyzing activity were obtained at high frequency for both genes. Western blot analysis using an anti-XynA antibody indicated that XynAl and XynA2 were produced in these calli.     

Effects of carbon dioxide (CO2) laser perforation as skin pretreatment to improve sugar infusion process of frozen blueberries

Sugar infusion is a widely used osmotic treatment for fruit preservation, but the process is inherently slow and the waxy skins of some fruits hinder mass transfer during the process. This work examined the utility of perforation by a carbon dioxide (CO(2)) laser as a novel skin treatment to improve the infusion process. In 2 experiments, individually quick frozen (IQF) blueberries were subjected to varying degrees of laser perforation (3 levels of perforation density × 3 levels of perforation depth), and then infused stepwise with high fructose corn syrup (HFCS) to a final °Brix of 70 using varying solution concentration increment (5, 10, 20, and 30 °Brix/d). At each concentration, increasing perforation density and depth promoted solute migration into the fruit with increased fruit weight (P < 0.05; up to 24.15%, 37.23%, 52.89%, 65.34% wt. increase at 5, 10, 20, and 30 °Brix/d compared to the controls). Laser-treated blueberries maintained the original shape without excessive shrinkage and texture hardening due to enhanced solute incorporation, while the controls and mechanically treated samples were ruptured and wrinkled at the end of the process. Increasing solution concentrations shortened the process duration but decreased final fruit weight due to greater osmotic gradients. However, negative effects of using higher solution concentrations on final fruit weight were significantly alleviated with moderate-to-high doses of laser perforation (P < 0.001). Overall, the results demonstrate that laser perforation can be a viable skin pretreatment technique, offering marked improvement on final process yield, process efficiency, and product quality. PRACTICAL APPLICATION: CO(2) laser perforation as a novel skin pretreatment for sugar infusion of individually quick frozen (IQF) blueberries is presented. The technique markedly improves the product yield and quality. Although further investigation is needed, the method may potentially be used for other waxy skin fruits such as cranberries and cherries.     

Fabrication of a microelectromechanical system mirror array and its drive electrodes for low electrical interference in wavelength‐selective switches

This article describes the fabrication of a microelectromechanical system (MEMS) mirror array and mirror‐drive electrodes with high‐aspect‐ratio gold walls that reduce electrical interference in a wavelength‐selective switch (WSS). The MEMS mirror array, in which a lot of closely spaced adjacent mirrors are electrostatically operated, can be fabricated with a high yield by encapsulating the mirrors with an organic film that protects them from process damage. The gold walls with a high‐aspect ratio are formed in the narrow space between adjacent mirror‐drive electrodes by using thick‐multilevel interconnection technology. Because of these walls between adjacent electrodes, each MEMS mirror operates with low electrical interference. © 2011 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.     

Prenatal Molecular Hydrogen Administration Ameliorates Several Findings in Nitrofen-Induced Congenital Diaphragmatic Hernia

Oxidative stress plays a pathological role in pulmonary hypoplasia and pulmonary hypertension in congenital diaphragmatic hernia (CDH). This study investigated the effect of molecular hydrogen (H₂), an antioxidant, on CDH pathology induced by nitrofen. Sprague-Dawley rats were divided into three groups: control, CDH, and CDH + hydrogen-rich water (HW). Pregnant dams of CDH + HW pups were orally administered HW from embryonic day 10 until parturition. Gasometric evaluation and histological, immunohistochemical, and real-time polymerase chain reaction analyses were performed. Gasometric results (pH, pO_2, and pCO₂ levels) were better in the CDH + HW group than in the CDH group. The CDH + HW group showed amelioration of alveolarization and pulmonary artery remodeling compared with the CDH group. Oxidative stress (8-hydroxy-2′-deoxyguanosine-positive-cell score) in the pulmonary arteries and mRNA levels of protein-containing pulmonary surfactant that protects against pulmonary collapse (surfactant protein A) were significantly attenuated in the CDH + HW group compared with the CDH group. Overall, prenatal H₂ administration improved respiratory function by attenuating lung morphology and pulmonary artery thickening in CDH rat models. Thus, H₂ administration in pregnant women with diagnosed fetal CDH might be a novel antenatal intervention strategy to reduce newborn mortality due to CDH.     

Gluten Gel and Film Properties in the Presence of Cysteine and Sodium Alginate

ABSTRACT:  Wheat flour has an ability of forming dough by mixing with water, which exhibits a rheological property required for making bread. The major protein is gluten, which is a valuable protein material for food industry. In this study, gluten protein gels and films were formed with cysteine and sodium alginate. Adding cysteine improved gel and film properties (stress relaxation behavior, bending strength). The gel containing 0.01 M cysteine had a longer relaxation time and was more rigid than the gel without cysteine. Although adding sodium alginate to the gluten suspension containing cysteine improved the water-holding ability and homogeneity of the gel network, the film from this gel was more brittle than the gluten film with cysteine alone. Microstructural observations of the gels and films with scanning electron microscopy suggested that water evaporation was more heterogeneous from the gel containing sodium alginate than from the gel with cysteine alone. Fourier transform-infrared (FT-IR) analysis during film formation suggested that the presence of cysteine encourages interaction between gluten molecules and results in intermolecular β-sheet formation in earlier stages than in the no additive condition. FT-IR results also suggested that the combined effect of sodium alginate and cysteine on the protein secondary structure was remarkably different from that of cysteine alone. Our results suggest that addition of a suitable amount of cysteine (0.01 M) and heat treatment to 80 °C during gluten gel and film formation induces a homogenous network in the gel and film by regulating disulfide–sulfide interactions.     

Polishing of single point diamond tool based on thermo-chemical reaction with copper

This paper describes a new polishing method for diamond cutting tools. The method is based on the principle of oxidization of copper and deoxidization of copper oxide by carbon. A diamond tool was brought into contact with a copper plate, heated in air to a range of 323–523 K. The depth of the removed layer of diamond increased almost linearly with contact time and reached approximately 7 nm after 6 h. In this erosion process, pre-existing microcracks on the diamond surface were reduced. In comparison with the mechanically polished tool, the thermo-chemically polished tool was highly resistant to chipping and yielded a significant rise in tool life.     

Low-temperature synthesis of barium titanate thin films by nanoparticles electrophoretic deposition

The nanoparticles electrophoretic deposition (EPD) of barium titanate (BaTiO₃ or BTO) thin films was investigated. BTO nanocrystallites in a pseudocubic perovskite phase with an average particle size of about 10 nm were synthesized at a low temperature of 90°C by a high-concentration sol–gel process. By using a mixed solvent of 2-methoxyethanol and acetylacetone as dispersing medium, transparent and well-dispersed BTO nanocrystallites suspensions within the concentration range of 0.0125 to 0.20 mol/l was successfully prepared for nanoparticles EPD. A uniform microstructure and a smooth surface were observed on the deposited films. The film thickness of the deposited films increased rapidly with increasing EPD time in the initial period of EPD, and thereafter gradually increased to a limited thickness. With increasing applied EPD voltage, the limited film thickness increased. A near linear relation between the film thickness of films and the concentration of suspensions was observed under the same EPD conditions. The microstructures of the deposited BTO thin films were investigated.     

Recovery characteristics of a YBCO high-temperature superconducting current-limiting element for use in low-voltage distribution systems

Recovery characteristics of a YBa₂Cu₃O_7-y high-temperature superconductor after its quenching are investigated experimentally in this paper. The zigzag-shape superconducting elements 310 mm in effective length and 1.5 mm × 1 mm in cross section are examined. AC current injection tests are carried out. It is clarified that the high-temperature superconducting element has a quick transition characteristic between the super- and normal-conducting states. The test elements recover to zero impedance state from high impedance state just after removing the over-current when the magnitude of accumulated heats due to the over-current during the quenching period is less than 1 J. Furthermore, it is found from experiments that the critical current level might be reduced by some self-magnetization due to a high current during the quenching period.     

Quenching current level coordination in superconducting power transmission systems

In the present power transmission system, electric power devices are equipped under the concept of insulation coordination and are protected from lightning overvoltage and switching surge by the arrester. However, the future superconducting power transmission system is characterized by the heavy current transmission capability, the lower transmission loss and lightning-surge-free circumstances. Thus, in the superconducting power transmission system, superconducting devices should be protected rather from quenching by the overcurrent of a short-circuit fault than from the breakdown by over-voltage. In this paper, a new concept of the coordination for the superconducting transmission system is proposed as a “quenching current level coordination” instead of the insulation coordination. Furthermore, it is pointed out that the quenching current level coordination has the duality relationship with the present insulation coordination. A superconducting fault current limiter should play an important part of the quenching current level coordination to prevent the other superconducting devices from quenching.