Compensation algorithm for the phase-shift error of polarization-based parallel two-step phase-shifting digital holography

We propose an algorithm for compensating the phase-shift error of polarization-based parallel two-step phase-shifting digital holography, which is a technique for recording a spatial two-step phase-shifted hologram. Although a polarization-based system of the technique has been experimentally demonstrated, there had been the problem that the phase difference of two phase-shifted holograms had been changed by the extinction ratio of the micropolarizer array attached to the image sensor used in the system. To improve the performance of the system, we established and formulated an algorithm for compensating the phase-shift error. Accurate spatial phase-shifting interferometry in the system can be conducted by the algorithm regardless of phase-shift error due to the extinction ratio. By the numerical simulation, the proposed algorithm was capable of reducing the root mean square errors of the reconstructed image by 1/4 and 1/5 in amplitude and phase, respectively. Also, the algorithm was experimentally demonstrated, and the experimental results showed that the system employing the proposed algorithm suppressed the conjugate image, which slightly appeared in the image reconstructed by the system not employing the algorithm, even when the extinction ratio was 10:1. Thus, the effectiveness of the proposed algorithm was numerically and experimentally verified.     

A PCR assay for detection of acetic acid-tolerant lactic acid bacteria in acidic food products

A PCR assay for the detection of acetic acid-tolerant lactic acid bacteria in the genera of Lactobacillus and Pediococcus was developed in this study. Primers targeting the bacterial 16S rRNA gene were newly designed and used in this PCR assay. To determine the specificity of the assay, 56 different bacterial strains (of 33 genera), 2 fungi, 3 animals, and 4 plants were tested. Results were positive for most tested bacterial members of 16S rRNA gene-based phylogenetic groups (classified in the Lactobacillus casei and Pediococcus group), including Lactobacillus fructivorans, Lactobacillus brevis, Lactobacillus buchneri, Lactobacillus plantarum, and Lactobacillus paracasei. For all other bacterial strains and eukaryote tested, results were negative. Bacterial DNA for PCR was prepared with a simple procedure with the use of Chelex 100 resin from culture after growth in deMan Rogosa Sharpe broth (pH 6.0). To test this PCR assay for the monitoring of the acetic acid-tolerant lactic acid bacteria, L. fructivorans was inoculated into several acidic food as an indicator. Before the PCR, the inoculation of 10 to 50 CFU of bacteria per g of food was followed by a 28-h enrichment culture step, and the PCR assay allowed the detection of bacterial cells. Including the enrichment culture step, the entire PCR detection process can be completed within 30 h.     

Survey of pesticide residues in imported frozen vegetables and fruits (1989.4~2008.3)

A survey of pesticide residues in 595 imported frozen products on the Tokyo market from April 1989 to March 2008 was carried out. Forty three kinds of pesticides, including organophosphorus, organochlorine, carbamate, pyrethroid and others, were detected between levels of trace (below 0.01 ppm) and 4.6 ppm from 162 samples. Chlorpyrifos, cypermethrin and omethoate were frequently detected in green vegetables (komatsuna leaf and spinach), cypermethrin and methamidophos were detected in pods and seeds (green soybean and string pea), chlorpropham (CIPC) was detected in potato, and captan and carbaryl were detected in berries (blueberry, raspberry and strawberry). The hydrophilic pesticide methamidophos was detected in flesh of lychee. Residue levels of these pesticides were calculated as between less than 0.5% and 30% of their ADI values according to the daily intake of frozen products. Therefore, these frozen products should be safe when they were eaten in customary amounts.     

A study of transfer switching for the purpose of fault detection in FRIENDS

The authors have proposed a new concept of a distribution system “Flexible, Reliable and Intelligent ENergy Delivery System (FRIENDS)” with intent to solve imminent operating problems faced by utilities in providing their customers with reliable supply of power at several levels of quality. The main idea of FRIENDS is to introduce new facilities, so‐called Quality Control Centers (QCCs), which are installed very close to the customers for the purpose of implementing multiple functions, such as Customized Power Quality Service. In addition, these QCCs form a network for energy and information transmission at a level below distribution substations. QCCs make it possible to frequently change configuration of the network depending on the system and load conditions. These frequent reconfigurations of the network require fast and reliable Transfer Switching in the QCCs to ease an effect on customers. The reconfiguration for the purpose of fault area isolation also requires a fast Fault Detection feature. This paper presents a new method for controlling Transfer Switching and a method of Fault Detection in order to realize a fast and reliable reconfiguration of QCC network in a normal state, as well as under fault conditions. This paper also analyzes the methods in terms of instantaneous values calculated by PSCAD/EMTDC. © 2004 Wiley Periodicals, Inc. Electr Eng Jpn, 148(3): 25–35, 2004; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.10298     

Characterization of the diacylglycerol acyltransferase activity in the membrane fraction from a fungus

In an attempt to clarify the mechanism of lipid accumulation inMortierella ramanniana var.angulispora, diacylglycerol acyltransferase (DGAT) in the membrane fraction from this fungus was characterized. The enzyme had an optimum pH of 7.0–7.5, and enzyme activity was blocked by SH-reagents. Metal ions were not essential for maintaining DGAT activity.n-Octyl-β-d-glucoside, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate and Tween 80 were found to preserve activity, while Triton X-100 and sucrose monolaurate inhibited it. As the inhibition of DGAT activity by Triton X-100 was overcome by the addition of diacylglycerol (DG), the dependency of DGAT activity on exogenous DG was determined in the presence of 0.1% Triton X-100. DGAT activity in the membrane fraction was traced in fungi cultured for different time periods or in media at different carbon to nitrogen (C/N) ratios. Although the increase in total lipid content with culture time was accompanied by an increase in DGAT activity, total lipid changes related to changes in C/N ratio did not correlate with DGAT activity. Factors other than DGAT activity in the membrane fraction would appear to be involved in the regulation of total lipid content in this fungus.     

Improvement of Selectivity for Partial Hydrogenation of Benzene by Rare Earth Nitride Upon NH3 Treatment

For the liquid-phase partial hydrogenation of benzene at 483 K, the selectivity toward cyclohexene was greatly improved upon treating rare earth nitride with ammonia. For YbN obtained by thermal decomposition of Yb(NH₂)₂ at 1173 K, the selectivity of the NH₃-treated YbN was 75%, in contrast to nil for the untreated YbN.     

Photocatalytic Fiber with Gradient Surface Structure Produced from a Polycarbosilane and Its Applications

We developed a new in-situ process for preparing functional ceramic fibers with a gradient surface layer by means of precursor methods using a polycarbosilane. After incorporation of selected low-molecular-mass additives into the precursor polymer from which the ceramic forms, thermal treatment of the resulting bodies leads to controlled phase separation ("bleed out") of the additives. Subsequent calcination stabilizes the compositionally changed surface region, generating a functional surface layer. Using this technology, we developed a strong photocatalytic fiber (TiO₂-covered SiO₂ fiber), which effectively decomposed many types of organic chemicals and bacteria into CO₂and water by irradiation with UV light. Furthermore, we performed some field tests using a circulation purifier with a module composed of the cone-shaped felt material of our new fiber.     

Remarkable effect of addition of In and Pb on the reduction of N2O by CO over SiO2 supported Pd catalysts

The effect of addition of In and Pb on the reduction of N₂O by CO was studied over SiO₂ supported Pd catalysts, using a closed gas circulation system as well as in-situ infrared spectroscopy. Formation of intermetallic compounds such as Pd_0.48In_0.52, Pd₃Pb and Pd₃Pb₂ was observed which caused a drastic enhancement of the rate of N₂ formation. The infrared spectroscopic analyses revealed a weakening of the adsorption strength of CO on Pd metal by the formation of intermetallic compounds, which is likely the main reason for the enhancement of the reaction rate. From a kinetic investigation as well as in situ FT-IR observation during the N₂O-CO reaction, a redox mechanism was proposed involving the oxidation of the surface by N₂O followed by its reduction by CO. Over Pd/SiO₂, the former process seems to be the rate limiting step because of the inhibition of N₂O activation by strongly adsorbed CO. By adding In or Pb, the rate limiting step shifted to the latter process, which resulted in a large enhancement in the rate of N₂ formation.     

Efficient production of L‐lactic acid by Crabtree‐negative yeast Candida boidinii

Industrial production of L ‐lactic acid, which in polymerized form as poly‐lactic acid is widely used as a biodegradable plastic, has been attracting world‐wide attention. By genetic engineering we constructed a strain of the Crabtree‐negative yeast Candida boidinii that efficiently produced a large amount of L ‐lactic acid. The alcohol fermentation pathway of C. boidinii was altered by disruption of the PDC1 gene encoding pyruvate decarboxylase, resulting in an ethanol production that was reduced to 17% of the wild‐type strain. The alcohol fermentation pathway of the PDC1 deletion strain was then successfully utilized for the synthesis of L ‐lactic acid by placing the bovine L ‐lactate dehydrogenase‐encoding gene under the control of the PDC1 promoter by targeted integration. Optimizing the conditions for batch culture in a 5 l jar‐fermenter resulted in an L ‐lactic acid production reaching 85.9 g/l within 48 h. This productivity (1.79 g/l/h) is the highest thus far reported for L ‐lactic acid‐producing yeasts. DDBJ/EMBL/GenBank nucleotide database with Accession Nos. AB440630 and AB440631. Copyright © 2009 John Wiley & Sons, Ltd.     

The result of cooling operation of Yazaki experimental solar house “one”

One of the important factors for designing solar house is to examine the most economic combination what is called optimum design between solar collector area and storage volume for the required energy demand. The result of experimentation gives the fundamental data for completing the computer simulation program that is effectively usable for designing solar house.