Poxvirus virions: their surface ultrastructure and interaction with the surface membrane of host cells

Virions of vaccinia and orf viruses were examined by ultrahigh-resolution scanning electron microscopy using a non-coating method. Intracellular mature particles of vaccinia virus appeared to be covered with a net and ultrastructurally their surface consists of many fine ridges and globules, while the surfaces of orf virus mature particles recovered from infected cells consist of spirally running protrusions. The ridge-like structures of vaccinia virus were presumed to correspond to surface tubules shown by negative staining of this virus, while the spiral protrusions of orf virus were presumed to correspond to spiral threads having a criss-cross appearance by the same staining. Using scanning electron microscopy in which the samples were prepared by the conventional method, we observed: (i) many virions, i.e. one or two hundreds, or occasionally more reaching about one thousand particles, of the IHD strain of vaccinia virus, (ii) many or a moderate number of virions, i.e. about one hundred or fewer particles, of the 58 strain of cowpox virus and (iii) rather few virions, i.e. several tens or fewer particles, of the Iwate strain of orf virus on the free surface of each cell infected with these viruses. It must be noted that the number of virions detected considerably differed in respective cells examined. Virus budding was frequently observed at the cell surface of monolayer cells infected with vaccinia virus but it was never detected with cowpox or orf virus, indicating a difference in the mechanism of virus release between vaccinia and the other two viruses. When whole cells infected with vaccinia virus were examined by a combination of high-voltage and scanning electron microscopies, virions on the cell surface and those inside the cells were clearly differentiated. All virions on the cell surface had an envelope, and some of the envelopes had a slack and/or one or more bulges.     

Trehalose embedding technique for high-resolution electron crystallography: application to structural study on bacteriorhodopsin

Trehalose was compared with glucose in its use as an embedding medium for the preparation of two-dimensional crystals for electron crystallographic structure analysis. Purple membranes (two-dimensional crystals of bacteriorhodopsin) embedded in either sugar were studied in a 'partially hydrated' state as well as after 'drying' of the specimen. Diffraction patterns of the four conditions, all showing spots to a resolution better than 3.0 A, were analysed and merged. In the case of glucose embedded specimens, the merging R-factor obtained from the diffraction patterns of dried specimens was significantly better than the one obtained from partially hydrated specimens. In the case of trehalose embedding, the merging R-factor for partially hydrated specimens was significantly better than the dried one. The trehalose embedded, partially hydrated specimens gave the best merging R-factor in all four cases.     

Cryogenic performance of double-fused 1.5-μm vertical cavitylasers

The low-temperature performance of vertical cavity lasers (VCL's) is of interest for high-speed data transmission from superconducting and cryogenic semiconductor circuits. Our double-fused 1.5 μm lasers employ a strain-compensated InGaAsP-InP multiquantum-well (MQW) active region that is sandwiched between two AlGaAs-GaAs distributed Bragg reflectors. Continuous wave (CW) lasing at ambient temperature as low as 7 K is measured on the same type of top-emitting devices that previously lased at a record-high temperature of 337 K. The optimum temperature is found at 180 K giving minimum threshold current, maximum modulation bandwidth of 5 GHz, and more than 3 GHz/mA^1/2 modulation current efficiency. The optimum temperature agrees very well with the theoretical prediction. Further device optimization for cryogenic high-speed applications is discussed in detail     

Design and demonstration of polarization-insensitive Mach-Zehnderswitch using a lattice-matched InGaAlAs/InAlAs MQW and deep-etchedhigh-mesa waveguide structure

The authors have designed and demonstrated a 2×2 Mach-Zehnder switch in view of polarization independence as well as low propagation loss (α) and absorption change (Δα). To obtain polarization-insensitive refractive index change (Δn), a lattice-matched InGaAlAs-InAlAs multiple quantum-well (MQW) with a large detuning wavelength was used. Moreover, to reduce the insertion loss difference between polarizations, we applied a multimode-interferometer 3-dB coupler and a deep-etched high-mesa waveguide structure. This switch, therefore, can provide polarization-independent operation about both driving voltage and insertion loss, which is indispensable to practical optical switching applications. We also paid attention to Δα suppression when we decided the value of wavelength detuning and the length of the phase shift region. We also investigated the wavelength dependence of the switch. Within 1530-1560 nm, which is the erbium-doped fiber amplifier (EDFA) gain band, polarization independence in the driving voltage and the crosstalk was maintained. This result shows that the switch is also applicable in wavelength division multiplexing (WDM) applications     

Micro inspection robot for 1-in pipes

A micro inspection robot for 1-in pipes has been developed. The robot is 23 mm in diameter and 110 mm in length and is equipped with a high-quality micro charge-coupled device (CCD) camera and a dual hand for manipulating small objects in pipes. It can travel through both vertical pipes and curved sections, making possible inspections that would be difficult with conventional endoscopes. Its rate of travel is 6 mm/s and it has a load-pulling power of 1 N. To realize this microrobot, the authors have specially designed and developed several micro devices and micromechanisms: a novel micromechanism called a planetary wheel mechanism for robot drive; a micro electromagnetic motor with a micro planetary reduction gear to drive the planetary wheel mechanism; a micro pneumatic rubber actuator that acts as a hand; a micro CCD camera with high resolution; and a pneumatic wobble motor for rotating the camera and hands. In the paper, the design and performance of these micro devices are reported, the performance of the robot as a whole is described, and an application example is given     

Pausible clocking-based heterogeneous systems

This paper describes a novel communication scheme, which is guaranteed to be free of synchronization failures, amongst multiple synchronous and asynchronous modules operating independently. In this scheme, communication between every pair of modules is done through an asynchronous first-in first-out (FIFO) channel; communication between a module and the FIFO is done using a request/acknowledge handshaking. Synchronization of handshake signals to the local module clock is done in an unconventional way-the local clock built out of a ring oscillator is paused or stretched, if necessary, to ensure that the handshake signal satisfies setup and hold time constraints with respect to the local clock. In order to validate this scheme, we implemented a test chip in 0.5-μm CMOS. This chip is designed as a ring, composed of two synchronous modules, an asynchronous module, and two asynchronous FIFOs. Each module functions as a receiver to one module and a sender to another module. Test results show that the chip functions reliably up to 456 MHz     

Sub‐Millimeter‐Scale Monolayer p‐Type H‐Phase VS2

2D H‐phase vanadium disulfide (VS₂) is expected to exhibit tunable semiconductor properties as compared with its metallic T‐phase structure, and thus is of promise for future electronic applications. However, to date such 2D H‐phase VS₂ nanostructures have not been realized in experiment likely due to the polymorphs of vanadium sulfides and thermodynamic instability of H‐phase VS₂. Preparation of H‐phase VS₂ monolayer with lateral size up to 250 µm, as a new member in the 2D transition metal dichalcogenides (TMDs) family, is reported. A unique growth environment is built by introducing the molten salt‐mediated precursor system as well as the epitaxial mica growth platform, which successfully overcomes the aforementioned growth challenges and enables the evolution of 2D H‐phase structure of VS₂. The honeycomb‐like structure of H‐phase VS₂ with broken inversion symmetry is confirmed by spherical aberration‐corrected scanning transmission electron microscopy and second harmonic generation characterization. The phase structure is found to be ultra‐stable up to 500 K. The field‐effect device study further demonstrates the p‐type semiconducting nature of the 2D H‐phase VS₂. The study introduces a new phase‐stable 2D TMDs materials with potential features for future electronic devices.     

中国卫通:创新卫星通信产业,致力数字集群服务

中国卫星通信集团公司(以下简称中国卫通)是根据国务院电信体制改革的总体部署,于2001年12月19日正式挂牌成立的国有大型骨干企业,是我国六大基础电信运营企业之一。中国卫通下设30个省级分公司和17个全资、控股、参股企业。作为我国卫星通信广播电视运营的主导企业,中国卫通以保障国家卫星通信广播安全、服务国民经济和社会发展为已任,不断以特色化通信保障国家信息网络安全,服务信息社会。特别是在卫星广播电视传输、应急指挥调度通信等特殊领域和边远农村、海上通信、自然灾害、突发事件等特殊区域,发挥了不可替代的作用,在为缩小数字鸿沟助力,构建和谐信息社会的进程中做出了重要贡献。     

时域与空域结合实现光束匀滑的光谱优化分析

为改善焦斑的均匀性以应用于惯性约束聚变(ICF)的实验,从焦斑形态的控制出发,在随机相位板(RPP)和连续相位板(CPP)做空域整形下,用同样带宽和最大谱色散角,模拟了不同光谱形状的匀滑效果,通过焦斑强度的功率谱密度(PSD)和平顶的均方根(RMS)对获得的焦斑进行评价.结果表明,改善光谱形状可以提高匀滑效率,优化光谱相对主流的正弦调频光谱对匀滑效果的均方根值可提高43.7%.同时,设计相位板时考虑焦斑上不同空间频率的权重,有利于匀滑.     

基于相干激光雷达的激光微多普勒探测

利用相干激光雷达探测目标微动特性技术是一种以单频激光为光源,用外差探测的方式实现对低速、低频多个运动物体进行微多普勒信息提取和识别的技术.以波长为1.064 μm的窄线宽单块激光器为光源,发射激光经过模拟长距离传输的4 km的光纤延迟,照射到以扬声器的发声单元和电动平移台为目标的微动物体上,目标反射的激光会聚进入单模光纤,参考光与信号光通过3 dB光纤合束器线性耦合进入同一根光纤,并在探测器表面进行相干.用于接收相干信号的探测器的接入方式为光纤输入,带宽为3.5 GHz.利用时间-频率域联合描述的方法对数/模(A/D)采样后的数据进行分析.在4 km光纤延迟时,本系统最低探测速度为0.5 mm/s,速度分辨率达到毫米每秒量级,频率分辨率达到千赫兹量级.利用微多普勒信息探测技术,实现了探测物体表面的微动状态信息和识别运动状态的目的.