电火花强化工艺制造金刚石镀层实验研究

为实现金刚石工具的快速制造和在线快速修复,探索了一种简单快速的金刚石表面强化工艺方法的可行性。以金刚石颗粒和钴颗粒为主要成分,采用压制工艺制作电极,并用该电极在基体材料304不锈钢板上进行电火花强化实验,探究电极的压制压力、金刚石含量、加工电流和加工时间等参数对实验效果的影响规律。用镀钛金刚石电极加工5 min,可得到表面上金刚石颗粒的表面积分数为13.5%、厚度为400μm的强化层,且该强化层具有很好的磨削性能。     

DEVELOPMENT OF A CHEMILUMINESCENT ELISA FOR DETERMINING OKADAIC ACID IN SHELLFISH

ABSTRACT

An indirect competitive enzyme‐linked immunosorbent assay with chemiluminescent (CL‐ELISA) detection for okadaic acid (OA) in mussel muscle was developed. A hybridoma cell line secreting monoclonal antibody against OA was established after immunization of BALB/c mice with artificially synthesized OA‐bovine serum albumin as antigen. Luminol solution was used as the substrate for horseradish peroxidase. The detection limit was 0.175 ng/g. The range of average fortified recovery was 91.8–102.6% when OA was spiked in mussel muscle at levels of 50, 160 and 800 µg/kg. The standard curve for OA showed good linearity over the concentration range of 0.08125–20 ng/mL. The cross‐reactivity with dinophysistoxin1 and saxitoxin was 51.82 and 0%, respectively. In a residue study, the results obtained by CL‐ELISA correlated well within those obtained using the commercial ABRAXIS kit (ABRAXIS, Warminster, PA). The developed method is therefore suitable for detecting the residues of OA in shellfish.

PRACTICAL APPLICATIONS

Diarrhetic shellfish poisoning is a gastrointestinal syndrome that occurs in humans following the consumption of bivalve mollusks contaminated with OA with the main symptoms of diarrhea, nausea, vomiting and abdominal pain. OA widely exists at marine products and it is very important to develop a technique to monitor this toxin. In this study, a sensitive competitive indirect enzyme‐linked immunosorbent assay with chemiluminescence for determination of OA in mussel soft tissues was investigated. Chemiluminescent ELISA (CL‐ELISA) is a good alternative method for screening samples. This technique has the potential to improve the sensitivity of the immunoassays by at least two to three orders of magnitude compared with conventional colorimetric detection.     

1000 MW机组锅炉汽动引风机运行试验分析

为节约厂用电、减少设备维护成本、简化引风机的DCS控制方式,对某1000 MW机组进行了引风机与脱硫增压风机合并后采用小汽轮机驱动引风机的改造。在汽动引风机调试过程中进行汽动引风机并入运行、最大出力试验,并对启动过程中的风机抢风现象给出了操作建议。     

Fe_2O_3含量对MnZn铁氧体中Fe~(2+)量、磁性能及导电机制的影响

通过测量MnZn铁氧体的磁性能及Fe2+、Mn3+含量,考察了MnZn铁氧体中的Fe2+含量与配方中Fe2O3、MnO含量的关系及其对MnZn铁氧体磁性能的影响,并探究了MnZn铁氧体的导电机制。结果表明:随着(Fe2O3)a(MnO)b(ZnO)c主组成配方中a值递增(52.55≤a≤53.35)、b值递减(38.33≥b≥37.52),呈现出Fe2+、Mn3+含量均增加的趋势。随着Fe2+含量增加,Pcv谷底温度向低温方向移动,Pcv(min)先减后增,Pcv(20/70/100℃)均先减后增,均在Fe2+含量=1.55%附近达到最小值;起始磁导率μi(20/70/100℃)均先增后减。根据Pcv-Fe2+含量和μi-Fe2+含量两个关系图在Fe2O3=53.15mol%附近出现极值点这一现象,初步推测铁氧体Znα2+Mnβ-x2+Mnx3+Fey2+Feχ-y3+O4+σ(0.1794≥α≥0.1786,0.754≥β≥0.734,0.0031≤x≤0.0040,0.051≤y≤0.070)的导电机制为:y0.064时小极化子间的束缚能主导,y0.064时电子跃迁主导。     

一起风电场变压器短路故障的诊断与分析

分析了一起风电变压器低压多股并绕导线间的短路故障案例,采用多种试验方法并结合测量数据分析,推断出了故障部位,详细的介绍了分析过程和检查情况,并提出预防措施。     

从工艺矿物学角度探讨某些难处理资源开发利用中的问题

探讨铜镍硫化矿矿石、钼矿石、大洋锰结核、脉石矿物中含弥散状磁铁矿矿石、含磁性硅酸盐矿物的赤铁矿矿石及某些含放射性矿物矿石等的开发利用过程中应注意的问题,并总结了影响矿石选矿的工艺矿物学因素。这样的总结可与自动工艺矿物学参数测试系统结合,形成资源可利用性评价的智能系统。     

生态脆弱矿区煤层覆岩隔水特征及保水开采实验研究

对薄基岩浅埋煤层覆岩的隔水性进行了分析,采用相似材料模拟的方法,研究了薄基岩浅埋煤层覆岩的物理力学特征和上覆岩层移动破坏规律。研究表明,覆岩的矿物成分以石英、钾长石等为主,其含量高达75.4%~92.0%;岩石的单向抗压强度远大于抗拉强度,属于典型的脆性岩石。风化岩石的膨胀性增强,塑性增加,强风化带内,结构致密的粉砂岩、细粒砂岩的物理力学性质无明显变化。相似模拟实验表明:在煤柱附近和采空区中央,沿顶板方向覆岩移动表现出明显的分区性,在同一层位水平变形引起的压缩区和拉伸区交错出现;导水断裂带最大高度约为94 m,是采高的27倍。在此基础上,对薄基岩浅埋煤层保水开采的条件进行了评价,提出了保水开采的关键技术措施。     

三参数Weibull分布下基于BRM的LED照明灯寿命预测

为了在短时间内准确获得LED照明灯的寿命信息,以3组恒定加速应力的试验数据为基础,采用三参数Weibull函数描述其寿命分布,基于双线性回归法(BRM)对试验数据进行处理分析,并利用自行开发的寿命预测软件较为精确地得出LED照明灯在正常工作应力下的寿命。数值结果表明,LED照明灯的寿命服从三参数Weibull分布,其加速模型符合Arrhenius方程,精确预测的LED照明灯寿命为工程技术人员关于产品的可靠性设计提供技术参考。     

含盐量对水泥灰土强度特性的影响

通过对水泥灰土不同含盐量试样进行一系列三轴不固结不排水剪切试验,探讨了含盐量对水泥灰土剪切强度参数的影响,并从微观结构性上做出了合理的解释.试验结果表明:水泥灰土强度随含盐量的增加而增大,但当含盐量达到一定程度时,其强度又随含盐量的增大而降低,且含盐量存在一个分界值;此外,水泥灰土的脆断性受围压和含盐量等因素的影响,围压越小,含盐量越高,脆性就表现得越明显.     

混凝-超滤组合工艺运行优化研究

采用混凝/浸没式超滤组合工艺对深圳某原水进行中试研究,从有机物去除和膜污染控制两方面对聚合氯化铝(PAC)投加量和膜池曝气强度进行了优化。结果表明,膜池气水比为12∶1,PAC投加量为0、3、4 mg/L时,Zeta电位绝对值逐渐减小,颗粒数和COD Mn去除效果增强,继续增加PAC投加量到5 mg/L,则Zeta电位绝对值增大,颗粒数和COD Mn去除效果变差。PAC投加量为4 mg/L,膜池气水比为(7.5∶1)、(9∶1)、(12∶1)时,Zeta电位绝对值逐渐减小,颗粒数和COD Mn去除效果逐渐增强,继续增大气水比到15∶1,则Zeta电位增大,颗粒数和COD Mn去除效果变差。PAC的投加和膜池曝气可减缓不可逆膜污染,增加PAC投加量可提高DOC去除率,降低单周期TMP增幅;提高曝气强度会降低DOC去除率,降低单周期TMP增幅。