浅析高层住宅建筑结构优化设计的策略

伴随着建筑行业一步步的发展,建筑材料工业以及建筑科学技术也随之发展,大多数人现在对高层建筑的舒适度及安全性越来越重视。在追求安全适用的前提下,做到成本最低,便成了投资者的目标,建筑结构优化设计也随之产生并发展。     

呼和浩特回族聚居区的保护与更新发展

本文以呼和浩特回族聚居区为切入点,分析其特色与保护价值。针对目前城市发展过程中存在的一系列问题,提出统一协调建筑特色、完善人性化设施、大力发展民族特色产业等观点。     

核电厂主螺栓超声检测与信号分析

依据RSE-M标准需要定期对核电厂反应堆压力容器(RPV)主螺栓进行超声检测,为了保证主螺栓螺纹区及光杆区不同深度刻槽的超声检测灵敏度,本文对检测工艺进行声场仿真计算,分析与判断数据采集中的相关信号与非相关信号,并重点分析裂纹信号的特征,验证了超声工艺的可靠性。结合现场实施案例,通过45°横波端角反射率高的特性,综合其他检测方法如涡流和渗透检测对缺陷性质进行判定,可有效确定异常信号。     

论照明灯具的接地问题

对《建筑照明设计标准》(GB50034-2004)第7.2.12条"当采用Ⅰ类灯具时,灯具的外露可导电部分应可靠接地"的规定作了系统阐述;说明了灯具及电气设备防触电分类的规定,及各类灯具(0、Ⅰ、Ⅱ、Ⅲ类)的特点和接地要求;阐述了低压配电系统电气设备(包括灯具)间接接触防护措施,以及和电气设备类别的关联。     

塔河油田碳酸盐岩油藏注气开发实践及选井认识

塔河油田碳酸盐岩油藏具有储集体埋藏深、非均质性强、储集空间变化大的特征,储集体类型以缝洞型油藏为主。随着油田的开发,能量和底水问题逐渐成为影响自然递减的主要因素。在水驱采油能力大幅下降的同时,注气提高采收率成为油田开发的重要手段。该文介绍在塔河油田注气过程中通过实践摸索,针对不同类型油藏注气开发的经验及规律,在注气选井原则提出新的认识,为碳酸盐油藏提高气驱效率、指导注气开发拓宽思路和方法。     

Effect of Humic Acid on Soil Physical and Chemical Properties,Microbial Community Structure,and Metabolites of Decline Diseased Bayberry

In recent years, bayberry decline disease has caused significant damage to the bayberry industry. In order to evaluate whether humic acid can be used to effectively control the disease, this research examined the nutritional growth and fruit quality of bayberry, soil physical and chemical properties, soil microbial community structure, and metabolites. Results indicated that the application of humic acid not only improved the vigor and fruit quality of diseased trees, but also increased the diversity of microbial communities in the rhizosphere soil. A great increase was observed in the relative abundance of bacterial genus Mycobacterium and Crossiella; fungal genus Fusarium and Coniosporium. In contrast, a significant decrease was observed in the relative abundance of bacterial genus Acidothermus, Bryobacter, Acidibacter, fungal genus of Geminibasidium and Mycena. Analysis of redundancies (RDA) for microbial communities and soil characteristics showed that the main four variables, including available nitrogen, phosphorus, potassium, and calcium, had a great effect on the composition of bacterial and fungal communities in bayberry rhizosphere soil at the genus level. The main four variables had a greater effect on bacterial communities than on fungal communities. In addition, ABC transporter, arginine and proline metabolism, galactose metabolism, and glutathione metabolism were significantly affected by humic acid, which changed the content of 81 metabolites including 58 significantly down-regulated metabolites such as isohexonic acid and carinitine, and 23 significantly up-regulated metabolites such as acidic acid, guaninosuccinate, lyxose, 2-monoolein, epicatechin, and pentonolactone. These metabolites also significantly correlated with rhizosphere soil microbiota at the phylum, order, and genus levels. In conclusion, the results demonstrated the role of humic acid on plant growth and fruit quality, as well as rhizosphere soil characteristics, microbiota, and secondary metabolites, which provides novel insights into the control of bayberry decline disease.     

2cChIP-seq and 2cMeDIP-seq: The Carrier-Assisted Methods for Epigenomic Profiling of Small Cell Numbers or Single Cells

Chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) can profile genome-wide epigenetic marks associated with regulatory genomic elements. However, conventional ChIP-seq is challenging when examining limited numbers of cells. Here, we developed a new technique by supplementing carrier materials of both chemically modified mimics with epigenetic marks and dUTP-containing DNA fragments during conventional ChIP procedures (hereafter referred to as 2cChIP-seq), thus dramatically improving immunoprecipitation efficiency and reducing DNA loss of low-input ChIP-seq samples. Using this strategy, we generated high-quality epigenomic profiles of histone modifications or DNA methylation in 10–1000 cells. By introducing Tn5 transposase-assisted fragmentation, 2cChIP-seq reliably captured genomic regions with histone modification at the single-cell level in about 100 cells. Moreover, we characterized the methylome of 100 differentiated female germline stem cells (FGSCs) and observed a particular DNA methylation signature potentially involved in the differentiation of mouse germline stem cells. Hence, we provided a reliable and robust epigenomic profiling approach for small cell numbers and single cells.     

Rationale and Clinical Research Progress on PD-1/PD-L1-Based Immunotherapy for Metastatic Triple-Negative Breast Cancer

Metastatic triple-negative breast cancer (mTNBC), a highly aggressive and malignant tumor, currently lacks an effective treatment. There has been some progress in the treatment of mTNBC with programmed death receptor-1/programmed death ligand-1 (PD-1/PD-L1) immunotherapy in recent years. The combination of PD-1/PD-L1 inhibitors with other therapies is a noteworthy treatment strategy. Immunotherapy in combination with chemotherapy or small-molecule inhibitors still faces many challenges. Additionally, there are some new immunotherapy targets in development. We aimed to further evaluate the effectiveness and usefulness of immunotherapy for treating mTNBC and to propose new immunotherapy strategies. This review explains the rationale and results of existing clinical trials evaluating PD-1/PD-L1 inhibitors alone or in combination for the treatment of mTNBC. For patients with aggressive tumors and poor health, PD-1/PD-L1 inhibitors, either alone or in combination with other modalities, have proven to be effective. However, more research is needed to explore more effective immunotherapy regimens that will lead to new breakthroughs in the treatment of mTNBC.