An Aminocaprolactam Racemase from Ochrobactrum anthropi with Promiscuous Amino Acid Ester Racemase Activity

The kinetic resolution of amino acid esters (AAEs) is a useful synthetic strategy for the preparation of single‐enantiomer amino acids. The development of an enzymatic dynamic kinetic resolution (DKR) process for AAEs, which would give a theoretical yield of 100 % of the enantiopure product, would require an amino acid ester racemase (AAER); however, no such enzyme has been described. We have identified low AAER activity of 15 U mg^?1 in a homologue of a PLP‐dependent α‐amino ?‐caprolactam racemase (ACLR) from Ochrobactrum anthropi. We have determined the structure of this enzyme, OaACLR, to a resolution of 1.87 Å and, by using structure‐guided saturation mutagenesis, in combination with a colorimetric screen for AAER activity, we have identified a mutant, L293C, in which the promiscuous AAER activity of this enzyme towards l ‐phenylalanine methyl ester is improved 3.7‐fold.     

Defective Oligodendroglial Lineage and Demyelination in Amyotrophic Lateral Sclerosis

Motor neurons and their axons reaching the skeletal muscle have long been considered as the best characterized targets of the degenerative process observed in amyotrophic lateral sclerosis (ALS). However, the involvement of glial cells was also more recently reported. Although oligodendrocytes have been underestimated for a longer time than other cells, they are presently considered as critically involved in axonal injury and also conversely constitute a target for the toxic effects of the degenerative neurons. In the present review, we highlight the recent advances regarding oligodendroglial cell involvement in the pathogenesis of ALS. First, we present the oligodendroglial cells, the process of myelination, and the tight relationship between axons and myelin. The histological abnormalities observed in ALS and animal models of the disease are described, including myelin defects and oligodendroglial accumulation of pathological protein aggregates. Then, we present data that establish the existence of dysfunctional and degenerating oligodendroglial cells, the chain of events resulting in oligodendrocyte degeneration, and the most recent molecular mechanisms supporting oligodendrocyte death and dysfunction. Finally, we review the arguments in support of the primary versus secondary involvement of oligodendrocytes in the disease and discuss the therapeutic perspectives related to oligodendrocyte implication in ALS pathogenesis.     

Effect of gamma‐irradiation on the mechanical behavior of EPDM rubber‐recycled newsprint microfibers composites

Waste newsprint paper was collected from the local market and subjected to chemical pulping using 2 M NaOH. The fiber, after getting rid of water, was treated again using 2 M HCl solution for the same time period. The obtained newsprint microfibers (NPFs) were characterized by using scanning electron microscopy (SEM), X‐ray diffraction (XRD), and Fourier transform infrared spectra. Then the dried and grounded NPF batch was mixed with ethylene propylene diene monomer (EPDM) rubber using different concentrations ranged from 5 to 50 phr. The prepared composites were irradiated by using gamma rays at different doses from 20 to 100 kGy. The mechanical properties of prepared EPDM/NPFs composites such as tensile strength (Ts), elongation at break (E_b%), tensile modulus (M₁₀₀), toughness (T_t), and crosslink density (Cd) were measured as a function of fiber contents and irradiation dose. The results indicated that the tensile strength (Ts) increases with increasing microfibers contents up to 10 phr and irradiation dose up to 40 kGy, while E_b% decreases as the fibers content and irradiation dose increase. M₁₀₀ and Cd values increase with increasing fibers content up 50 phr fibers and irradiation dose up to 60 kGy. The results also concluded that the toughness values of EPDM/NPFs composites reach its maximum degree when using 10 phr NPFs concentration and 60 kGy irradiation dose. J. VINYL ADDIT. TECHNOL., 25:198–212, 2019. © 2018 Society of Plastics Engineers     

A review on natural convective heat transfer of nanofluids

Nanofluids are considered to have great potential for heat transfer enhancement and are highly suited to application in practical heat transfer processes. Recently, several important studies were carried out to understand and explain the causes of the enhancement or control of heat transfer using nanofluids. The main aim upon which the present work is based is to give a comprehensive review on the research progress on the natural convective heat transfer characteristics of nanofluids for both single- and two-phase models. Both experimental and theoretical studies are reviewed for natural convection of nanofluids in different types of enclosures.     

Greedy Detection

In this paper, we introduce a new greedy algorithm developed for communication systems characterized with multiple simultaneous data transmission. Specifically, we consider code-division multiple-access (CDMA) systems and systems employing space-time coding (STC). The optimum detection in such systems has an exponential complexity and cannot be used in practical systems. We show that performance close to the optimum performance yet with significantly lower complexity can be achieved by the proposed algorithm. Also, we show that its performance is significantly better than that of most of the existing sub-optimum schemes for a wide range of operating conditions.     

The role of the hyporheic zone in the nitrogen dynamics of a semi‐arid gravel bed stream located downstream of a heavily polluted reservoir (Tafna wadi,Algeria)

Nitrogen retention was measured along the Tafna wadi downstream of a heavily polluted reservoir in North‐West Algeria to understand the role of the hyporheic zone (HZ) in nitrogen dynamics. Nutrient concentrations were measured monthly for 2 years within the bed sediments of a 300 m reach located 20 km downstream from the dam. Due to strong hydrological fluctuations hyporheic water was analysed during natural low and high water (HW) periods, and during water reservoir releases. Nutrient concentrations in surface water (SW) increased during water releases and in the HZ during the low water (LW) periods. Surface/hyporheic water interactions were characterized by determining the vertical hydraulic gradient (VHG) and the chemical signature of the ground water (GW). The latter was obtained from regional GW monitoring. Hyporheic chemistry was strongly influenced by patterns of surface flow. Hyporheic and SWs had similar chloride concentrations during high flow when they were significantly lower than those of the regional GW. GW was generally richer in nitrates and nitrites, but was lower in ammonium concentrations than interstitial and river waters. Nitrates decreased significantly from upstream to downstream within the HZ throughout the hydrological period even though temporal fluctuations were high. Ammonium concentrations in interstitial water (IW) were significantly higher than in SW and generally increased from upstream to downstream. This study demonstrates the importance of the HZ in altering the dissolved inorganic nitrogen composition and concentrations of heavily polluted arid streams. The study is of interest because it documents a large ‘natural experiment’ that being the effect of periodic water release from a reservoir with serious water quality problems on the water quality dynamics (particularly nitrogen) of subsurface and SWs downstream. Copyright © 2008 John Wiley & Sons, Ltd.     

Numerical Analysis of Turbulent Forced Convection of Nanofluid Flow over a Heated Shallow Cavity

This work presents a numerical investigation of turbulent forced convection of a nanofluid over a heated cavity in a horizontal duct. Heat transfers in separated flows are frequently encountered in engineering applications, such as: heat exchangers, axial and centrifugal compressor blades, gas turbines blades, and microelectronic circuit boards. Thus, it is very essential to understand the mechanisms of heat transfer in such regions in order to enhance heat transfer. Different volume fractions of nanoparticles are presented in the base fluid and different types of nanoparticles are used. The objective of this study is to check the effect of nanofluid on heat transfer in such a configuration. Numerical simulations are performed for pure water and four nanofluids (Cu, CuO, Ag, and Al₂O₃). The results are analyzed through the thermal and dynamical fields with a particular interest to the skin friction coefficient and Nusselt number evolutions. The average Nusselt number increases with the volume fraction of nanoparticles for the whole tested range of Reynolds number. A correlation of average Nusselt number versus Reynolds number and volume fraction of each type of nanoparticles over the cavity wall is proposed in this paper.     

Horizontal Holonomy for Affine Manifolds

In this paper, we consider a smooth connected finite-dimensional manifold M, an affine connection ? with holonomy group H ^? and Δ a smooth completely non integrable distribution. We define the Δ-horizontal holonomy group \({H^{\;\nabla }_{\Delta }}\) as the subgroup of H ^? obtained by ?-parallel transporting frames only along loops tangent to Δ. We first set elementary properties of \({H^{\;\nabla }_{\Delta }}\) and show how to study it using the rolling formalism Chitour and Kokkonen (2011). In particular, it is shown that \({H^{\;\nabla }_{\Delta }}\) is a Lie group. Moreover, we study an explicit example where M is a free step-two homogeneous Carnot group with m ≥ 2 generators, and ? is the Levi-Civita connection associated to a Riemannian metric on M, and show in this particular case that \({H^{\;\nabla }_{\Delta }}\) is compact and strictly included in H ^? as soon as m ≥ 3.     

Discontinued and alternative brominated flame retardants in the atmosphere and precipitation from the great lakes basin

Air (vapor and particle) and precipitation samples were collected at five sites (two urban, one rural, and two remote) around the Great Lakes during 2005-2009 as a part of the Integrated Atmospheric Deposition Network (IADN). The concentrations of polybrominated diphenyl ethers (PBDEs), decabromodiphenylethane (DBDPE), hexabromobenzene (HBB), pentabromoethylbenzene (PBEB), and 1,2-bis(2,4,6-tribromophenoxy)ethane (BTBPE) were measured in these samples. The highest concentrations of these compounds were generally observed at the two urban sites-Chicago and Cleveland-with a few exceptions: The remote site at Eagle Harbor had particularly high levels of PBEB in all three phases, and the rural Sturgeon Point site had the highest HBB concentrations in the vapor phase. The sources of HBB and PBEB to these sites are unknown. A multiple linear regression model was applied to the concentrations of these compounds in the vapor phase, particle phase, precipitation, and the three phases combined. This regression resulted in overall (three phases combined) halving times for total PBDE concentrations of 6.3 ± 1.1 years. The overall halving times for HBB and BTBPE were 9.5 ± 4.6 years and 9.8 ± 2.8 years, respectively. For PBEB and DBDPE, the regression was not statistically significant for the combined phases, indicating that the atmospheric concentrations of these compounds have not changed between 2005 and 2009.