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101.
The effects of midazolam, one of the most popular benzodiazepines, on synaptic transmissions were compared with intracellular recordings between CA1 pyramidal cells (CA1-PCs) and dentate gyrus granule cells (DG-GCs) in rat hippocampal slices. First, we studied the effects of midazolam on orthodromically evoked spikes, membrane properties and synaptic potentials. Secondly, the effects of a GABA(A) receptor agonist, muscimol, were examined on membrane properties to determine whether or not the densities of GABA(A) receptors are different between CA1-PCs and DG-GCs. Midazolam (75 microM) markedly depressed orthodromically evoked spikes in CA1-PCs, compared with those in DG-GCs. A GABA(A) receptor antagonist, bicuculline (10 microM), almost completely antagonized the depressant effects of midazolam on spike generation in CA1-PCs, whereas it had little effect on midazolam in dentate gyrus granule cells. Midazolam produced either depolarizing or hyperpolarizing effects on resting membrane potentials (Vm) with an input resistance decrease in CA1-PCs, whereas it produced depolarized Vm in DG-GCs. Midazolam significantly increased the amplitude of monosynaptic inhibitory postsynaptic potentials in CA1-PCs, whereas midazolam slightly decreased these in DG-GCs. Midazolam significantly decreased the amplitude of excitatory postsynaptic potentials both in CA1-PCs and DG-GCs. Muscimol (100 microM) produced either depolarizing or hyperpolarizing effects on Vm with an input resistance decrease in CA1-PCs, and it depolarized Vm with an input resistance decrease in DG-GCs. These results demonstrate that midazolam has differential effects on excitatory and inhibitory synaptic transmissions in hippocampal neurons. The mechanism of this difference could be partly due to the different types of GABA(A) receptors between CA1-PCs and DG-GCs. 相似文献
102.
103.
We describe a simple and effective approach to introduce a functional group into polymer film on a solid surface using reactive polymer LB films. N-dodecylacrylamide copolymers containing terminal amino groups in the side chains as the reactive moiety form a stable monolayer, and the monolayer was transferred onto a solid support to modify the solid surface using the Langmuir-Blodgett method. The transferred coatings were characterized with fluorescence, IR spectroscopies, and X-ray diffraction. The reactivity of the terminal amino group incorporated in the LB films was investigated in detail using fluorescein isothiocyanate (FITC) as a fluorescent probe. The chemical reaction between amino groups in the LB films and FITC in the bulk solution was completed within approximately 30 minutes and the chemical bond formation was confirmed by infrared spectroscopy. Furthermore, the fluorescent image of the multilayers reacted with FITC were observed with fluorescent microscopy. This method is effective for tailoring functional organic ultrathin films on solid substrates. 相似文献
104.
105.
Akazawa K. Makikawa M. Kawamura J. Aoki H. 《IEEE transactions on bio-medical engineering》1989,36(7):746-753
For developing functional neuromuscular stimulation (FNS) orthoses, percutaneous implants made of sintered hydroxyapatite (HAp) and a microprocessor-based portable stimulator were devised, and related basic studies were carried out. First, a button-shaped HAp was percutaneously implanted up to 23 months in the skin of dogs, and 30 months in human subjects. The HAp was closely contacted with the skin tissue. Then, button-shaped HAp electrical connectors were percutaneously implanted in the skin of rabbits. Sufficient contractions of the calf muscle were elicited by electrical stimuli delivered to intramuscular electrodes through the connector. These results indicated that the HAp electrical connector could be clinically utilized as an implantable skin interface for FNS. The microprocessor-based stimulator capable of processing control command signals and delivering 16-channels of electrical stimuli was developed. Usefulness of the stimulator was demonstrated by applying it to flexor and extensor muscles of the thumb in a C5 quadriplegic patient. 相似文献
106.
H. Aoki Y. Asada T. Hatano K. Ogawa A. Yanase M. Koiwa 《Journal of Low Temperature Physics》1990,81(1-2):19-29
Studies of the dHvA effect have been made on NbO single crystals grown by a zone melting techniques and 13 separate frequency branches ranging from 5.2×106 G to 1.2×108G have been observed. To understand the origin of the dHvA frequencies, we have made a self-consistent band structure calculation. Most experimental frequencies are found to be assigned to the extremal orbits on the calculated Fermi surfaces. There is good qualitative agreement between experiment and calculation, but some quantitative discrepancies still exist. We also report some physical properties, i.e., electrical resistivity, superconducting transition temperature, and electronic specific heat, of this high-quality NbO crystal. 相似文献
107.
One‐way delay variation (OWDV) has become increasingly of interest to researchers as a way to evaluate network state and service quality, especially for real‐time and streaming services such as voice‐over‐Internet‐protocol (VoIP) and video. Many schemes for OWDV measurement require clock synchronization through the global‐positioning system (GPS) or network time protocol. In clock‐synchronized approaches, the accuracy of OWDV measurement depends on the accuracy of the clock synchronization. GPS provides highly accurate clock synchronization. However, the deployment of GPS on legacy network equipment might be slow and costly. This paper proposes a method for measuring OWDV that dispenses with clock synchronization. The clock synchronization problem is mainly caused by clock skew. The proposed approach is based on the measurement of inter‐packet delay and accumulated OWDV. This paper shows the performance of the proposed scheme via simulations and through experiments in a VoIP network. The presented simulation and measurement results indicate that clock skew can be efficiently measured and removed and that OWDV can be measured without requiring clock synchronization. 相似文献
108.
McMullen T.W.P.; Li J.; Sheffield P.J.; Aoki J.; Martin T.W.; Arai H.; Inoue K.; Derewenda Z.S. 《Protein engineering, design & selection : PEDS》2000,13(12):865-871
The mammalian brain contains significant amounts of the cytosolicisoform Ib of the platelet-activating factor acetylhydrolase(PAF-AH), a unique type of PLA2. This oligomeric protein complexcontains three types of subunits: two homologous (63% identity)26 kDa catalytic subunits ( 相似文献
109.
Frequency and amplitude modulation (FM and AM) of a high-frequency (HF) de Haas-van Alphen oscillation due to magnetic interaction (MI) with a low-frequency (LF) oscillation in lead has been examined for field directions parallel to and close to 110, in the light of the old and of a new treatment of MI based on uniform and inhomogeneous inductions over the sample, respectively. The de Haas-van Alphen signals have been measured by the standard low-frequency field modulation method. The strength of AM and the sideband around HF have been examined as a function of the strength of FM and it is found that the experimental data deviate significantly from the prediction by the old treatment. Moreover, the deviation depends not only on the particular sample chosen to study, but also on the orientation, suggesting that the MI effect is influenced by the microscopic inhomogeneity of the sample. The absolute amplitude of LF also has been measured and is found again to deviate significantly from the prediction by the old treatment. However, the measured amplitude shows a satisfactory agreement with the prediction by the new treatment. In conclusion, the new treatment is a plausible theory which can describe all our experimental results, not only qualitatively but also quantitatively. 相似文献
110.
Ichinose S Muneta T Sekiya I Itoh S Aoki H Tagami M 《Journal of materials science. Materials in medicine》2003,14(1):79-86
We surgically retrieved two cobalt(Co)–chromium(Cr)–molybdenum(Mo) and five titanium(Ti)–aluminum(Al)–vanadium(V) alloy knee prostheses from patients because of mechanical failure and pain. We examined the distribution of the small particles which were released from the Co–Cr–Mo and Ti–Al–V alloys using a backscattered scanning electron microscopy (SEM). In addition we analyzed the metals in the artificial knee joints and the tissues adjacent to them using energy dispersive X-ray spectroscopy (EDS). We demonstrated that a myriad of fine particles, produced by the abrasion of both Co–Cr–Mo and Ti–Al–V alloys, accumulated in the synovial cells. As Co–Cr–Mo alloys disintegrate easily in the cells, Co dissolves from the peripheral areas of them, although Cr remains within the cells. In contrast Ti–Al–V alloys are very stable in the synovial cells. From these findings we conclude that the Co–Cr–Mo alloys are hazardous to the body as the alloys release Co which enters the body. In contrast the Ti–Al–V alloys are very stable and are patently safer. Artificial joints, however, are still in considerable need of improvement. 相似文献