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111.
112.
The effects of midazolam, one of the most popular benzodiazepines, on synaptic transmissions were compared with intracellular recordings between CA1 pyramidal cells (CA1-PCs) and dentate gyrus granule cells (DG-GCs) in rat hippocampal slices. First, we studied the effects of midazolam on orthodromically evoked spikes, membrane properties and synaptic potentials. Secondly, the effects of a GABA(A) receptor agonist, muscimol, were examined on membrane properties to determine whether or not the densities of GABA(A) receptors are different between CA1-PCs and DG-GCs. Midazolam (75 microM) markedly depressed orthodromically evoked spikes in CA1-PCs, compared with those in DG-GCs. A GABA(A) receptor antagonist, bicuculline (10 microM), almost completely antagonized the depressant effects of midazolam on spike generation in CA1-PCs, whereas it had little effect on midazolam in dentate gyrus granule cells. Midazolam produced either depolarizing or hyperpolarizing effects on resting membrane potentials (Vm) with an input resistance decrease in CA1-PCs, whereas it produced depolarized Vm in DG-GCs. Midazolam significantly increased the amplitude of monosynaptic inhibitory postsynaptic potentials in CA1-PCs, whereas midazolam slightly decreased these in DG-GCs. Midazolam significantly decreased the amplitude of excitatory postsynaptic potentials both in CA1-PCs and DG-GCs. Muscimol (100 microM) produced either depolarizing or hyperpolarizing effects on Vm with an input resistance decrease in CA1-PCs, and it depolarized Vm with an input resistance decrease in DG-GCs. These results demonstrate that midazolam has differential effects on excitatory and inhibitory synaptic transmissions in hippocampal neurons. The mechanism of this difference could be partly due to the different types of GABA(A) receptors between CA1-PCs and DG-GCs. 相似文献
113.
114.
Jungo Kondo Aoki K. Kondo A. Ejiri T. Iwata Y. Hamajima A. Mori T. Mizuno Y. Imaeda M. Kozuka Y. Mitomi O. Minakata M. 《Photonics Technology Letters, IEEE》2005,17(10):2077-2079
We present a novel thin-sheet X-cut LiNbO/sub 3/ optical modulator structure which can be fabricated by precise polishing and lapping to obtain a thinner LiNbO/sub 3/ substrate for a lower driving voltage in addition to velocity matching and impedance matching. We demonstrated that the fabricated modulator had a driving voltage V/spl pi/ of 2 V and zero chirp for 40-Gb/s operation and had a high potential for suppressed dc drift, and long-term reliability. 相似文献
115.
We describe a simple and effective approach to introduce a functional group into polymer film on a solid surface using reactive polymer LB films. N-dodecylacrylamide copolymers containing terminal amino groups in the side chains as the reactive moiety form a stable monolayer, and the monolayer was transferred onto a solid support to modify the solid surface using the Langmuir-Blodgett method. The transferred coatings were characterized with fluorescence, IR spectroscopies, and X-ray diffraction. The reactivity of the terminal amino group incorporated in the LB films was investigated in detail using fluorescein isothiocyanate (FITC) as a fluorescent probe. The chemical reaction between amino groups in the LB films and FITC in the bulk solution was completed within approximately 30 minutes and the chemical bond formation was confirmed by infrared spectroscopy. Furthermore, the fluorescent image of the multilayers reacted with FITC were observed with fluorescent microscopy. This method is effective for tailoring functional organic ultrathin films on solid substrates. 相似文献
116.
Hiromasa Tawarayama Etsuko Ishikawa Kenji Yamanaka Katsuhisa Itoh Keiko Okada Hiroshi Aoki Hiroaki Yanagita Yoshihiko Matsuoka Hisayoshi Toratani 《Journal of the American Ceramic Society》2000,83(4):792-796
Praseodymium-doped glasses were prepared in the Ga-Na-S (GNS) system and their optical properties were studied. A single-mode fiber with an attenuation loss of 1.2 dB/m at a wavelength of 1.31 μm was fabricated using an extrusion method, and the amplification characteristics were measured in the bidirectional pumping configuration. We demonstrated a gain coefficient of 0.81 dB/mW at a wavelength of 1.34 μm, which is the highest we have ever reported, and achieved a net gain of 32 dB for a pump power of 90 mW. Highly efficient optical amplification at a wavelength of 1.3 μm was demonstrated in the praseodymium-doped GNS fiber. 相似文献
117.
118.
Yuji Shimada Akio Sugihara Yumi Minamigawa Kenichi Higashiyama Kengo Akimoto Shigeaki Fujikawa Sadao Komemushi Yoshio Tominaga 《Journal of the American Oil Chemists' Society》1998,75(9):1213-1217
An attempt was made to enrich arachidonic acid (AA) from Mortierella single-cell oil, which had an AA content of 25%. The first step involved the hydrolysis of the oil with Pseudomonas sp. lipase. A mixture of 2.5 g oil, 2.5 g water, and 4000 units (U) Pseudomonas lipase was incubated at 40°C for 40 h with stirring at 500 rpm. The hydrolysis was 90% complete after 40 h, and the resulting
free fatty acids (FFA) were extracted with n-hexane (AA content, 25%; recovery of AA, 91%). The second step involved the selective esterification of the fatty acids with
lauryl alcohol and Candida rugosa lipase. A mixture of 3.5 g fatty acids/lauryl alcohol (1:1, mol/mol), 1.5 g water, and 1000 U Candida lipase was incubated at 30°C for 16 h with stirring at 500 rpm. Under these conditions, 55% of the fatty acids were esterified,
and the AA content in the FFA fraction was raised to 51% with a 92% yield. The long-chain saturated fatty acids in the FFA
fraction were eliminated as urea adducts. This procedure raised the AA content to 63%. To further elevate the AA content,
the fatty acids were esterified again in the same manner with Candida lipase. The repeated esterification raised the AA content to 75% with a recovery of 71% of its initial content. 相似文献
119.
Akazawa K. Makikawa M. Kawamura J. Aoki H. 《IEEE transactions on bio-medical engineering》1989,36(7):746-753
For developing functional neuromuscular stimulation (FNS) orthoses, percutaneous implants made of sintered hydroxyapatite (HAp) and a microprocessor-based portable stimulator were devised, and related basic studies were carried out. First, a button-shaped HAp was percutaneously implanted up to 23 months in the skin of dogs, and 30 months in human subjects. The HAp was closely contacted with the skin tissue. Then, button-shaped HAp electrical connectors were percutaneously implanted in the skin of rabbits. Sufficient contractions of the calf muscle were elicited by electrical stimuli delivered to intramuscular electrodes through the connector. These results indicated that the HAp electrical connector could be clinically utilized as an implantable skin interface for FNS. The microprocessor-based stimulator capable of processing control command signals and delivering 16-channels of electrical stimuli was developed. Usefulness of the stimulator was demonstrated by applying it to flexor and extensor muscles of the thumb in a C5 quadriplegic patient. 相似文献
120.
A metal/insulator/semiconductor (MIS) structure of Ga0.47In0.53As has been prepared by applying an excimer laser photo-CVD process to depositing an SiNx insulating layer. It is found that interface state densities can be remarkably reduced by in situ photochemical etching with CC14 or CH3Br gases prior to SiNx deposition. A minimum value of the U-shape profile of the interface state density as low as 5 × 1011 cm-2eV-1is attained. 相似文献