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71.
72.
Daniel Kirsten 《Theory of Computing Systems》2006,39(1):15-50
We introduce a new model of weighted automata: the desert automata. We show
that their limitedness problem is PSPACE-complete by solving the underlying
Burnside problem. As an application of this result, we give a positive
solution to the so-called finite substitution problem which was open for more
than 10 years: given recognizable languages K and L, decide whether there
exists a finite substitution σ such that σ(K) = L. 相似文献
73.
This article explores the use of a multimedia search interface for digital libraries based on strand maps developed by the American Association for the Advancement of Science. As semantic-spatial displays, strand maps provide a visual organization of relevant conceptual information that may promote the use of science content during digital library use. A study was conducted to compare users' cognitive processes during information seeking tasks when using a multimedia strand maps interface, versus the textual search interface currently implemented in the Digital Library for Earth System Education. Quantitative and qualitative data from think-aloud protocols revealed that students were more likely to engage with science content (e.g., analyzing the relevance of science concepts with regard to task needs) during search when using the strand maps interface compared to those using textual searching. In contrast, students using a textual search interface engaged more frequently with surface-level information (e.g., the type of a resource regardless of its science content) during search and retrieval. As a multimedia search interface for digital libraries, strand maps appear to be promising tools to promote conceptual discovery and learning through content-based processes that promote learner engagement with relevant science knowledge. 相似文献
74.
A review of the synthesis and characterization of a new type of stacked, bridged macrocyclic metal complexes, the (μ-cyano)phthalocyaninatometal compounds [PcMCN]n (2) is given. By using the routes described in Fig. 3 the polymers were synthesized with M = Co3+, Fe2+, Fe3+, Mn3+ and Cr3+ as the central metal ion. The compounds were characterized by IR, far-IR, UV, thermal and elemental analysis, and partly by 1H NMR and FD (field desorption) mass spectroscopy.[PcCoCN] (2a) and [PcFeCN]n (2b) exhibit d.c. room temperature conductivities around 10?2 S/cm without doping, thereby showing conductivities which are in the same range as the iodine-doped phthalocyaninatosiloxanes [PcSiO]n. 相似文献
75.
Soren Damgaard-Larsen Kirsten Ottar Jensen Ebba Lund Birte Nissen 《Water research》1977,11(6):503-508
Coxsackievirus and tritiated water were added to municipal sludges which were placed on lysimeters containing sandy soil or clay soil. It took 23 weeks during a normal Danish winter to inactivate virus from an initial 106tcid50/g sludge to a not demonstrable level. Irrespective of sludge and soil type no virus was demonstrated in the leachate water. The rainfall during December–May was 300 mm. 相似文献
76.
Breunig M Lungwitz U Klar J Kurtz A Blunk T Goepferich A 《Journal of nanoscience and nanotechnology》2004,4(5):512-520
For non-viral gene delivery, the carriers for DNA transfer into cells must be vastly improved. The branched cationic polymer polyethylenimine has been described as an efficient gene carrier. However, polyethylenimine was demonstrated to mediate substantial cytotoxicity. Therefore, this study is aimed at investigating per-N-methylated polyethylenimine, which is thought to have a much lower cytotoxicity due to its lower charge density. Results from a gel retardation assay and laser light scattering indicated that per-N-methylated polyethylenimine condenses DNA into small and compact nanoparticles with a mean diameter <150 nm. Furthermore, polyplexes of polyethylenimine and per-N-methylated polyethylenimine with DNA had a positive zeta potential and the polymers protected DNA from nuclease-mediated digestion. The transfection efficiency of polyethylenimine and per-N-methylated polyethylenimine was tested in CHO-K1 cells. Using green fluorescent protein as reporter gene and flow cytometry analysis, we demonstrated that per-N-methylated polyethylenimine has a lower cytotoxicity, but also a significantly lower transfection efficiency. Using propidium iodide staining, we could additionally distinguish between viable and dead cells. At NP > or = 12, per-N-methylated polyethylenimine showed a much higher cell viability and the ratio of viable and transfected cells to dead and transfected cells was about 1.5 to 1.7 fold higher than for polyethylenimine. The results of cell viability from flow cytometry analysis were confirmed by the MTS assay. Using luciferase reporter gene for transfection experiments, the gene expression of per-N-methylated polyethylenimine was lower at NP 6, 12 and 18 as compared to polyethylenimine, but at NP 24 it yielded similar levels. 相似文献
77.
Armin Bürkholz 《化学,工程师,技术》1979,51(12):1255-1255
78.
79.
The Cytosensor microphysiometer uses silicon chip technology to correlate changes in extracellular acidification rates with quantitative changes in cellular metabolism in response to ligand binding to surface receptors. This functional measure of physiology makes the Cytosensor a valuable tool in drug discovery research by allowing application of the instrument to screening of prospective pharmacologically active agents, characterizations of dose responses and structure-activity relationships, and investigation of mechanisms of action. 相似文献
80.
Rohrlack T Christoffersen K Hansen PE Zhang W Czarnecki O Henning M Fastner J Erhard M Neilan BA Kaebernick M 《Journal of chemical ecology》2003,29(8):1757-1770
This paper describes the purification and characterization of microviridin J, a newly discovered metabolite of Microcystis that causes a lethal molting disruption in Daphnia spp., upon ingestion of living cyanobacterial cells. Microviridin J consists of an acetylated chain of 13 amino acids arranged in three rings and two side chains. Unlike other known isoforms of microviridin, microviridin J contains arginine that imparts a unique solution conformation characterized by proximal hydrophobic interactions between Arg and other regions of the molecule. This eventually results in the formation and stabilization of an additional ring system. Microviridin J potently inhibits porcine trypsin, bovine chymotrypsin, and daphnid trypsin-like proteases. The activity against trypsin is most likely due to Arg and its distinctive conformational interactions. Overall, the data presented for microviridin J emphasize once again the ability of cyanobacteria to produce numerous and potent environmental toxins. 相似文献