Effect of dynamic cross-linking on phase morphology and dynamic mechanical properties of polyamide 12/ethylene vinyl acetate copolymer blends

Dynamic cross-linking of polyamide 12 (PA12) and ethylene vinyl acetate copolymer (EVA) blends in the mixing chamber of a torque rheometer was investigated. EVA was selectively cross-linked within the PA12 phase through free radical reactions using dicumyl peroxide. The torque level and temperature in the torque rheometer chamber were monitored to follow the evolution of the EVA cross-linking during the dynamic cross-linking process. The degree of cross-linking of EVA in the PA12/EVA materials was estimated based on the gel content (insoluble EVA fraction). The PA12/EVA phase morphology was investigated by scanning electron microscopy. The solid viscoelastic properties were investigated by dynamic mechanical thermal analysis (DMTA). The morphology, interfacial tension and viscoelastic results showed the immiscible nature of this system. The morphology of the blends was observed and the results revealed a two-phase system. The PA12/EVA 70/30 showed disperse-phase morphology, however a co-continuous phase was observed in blend ratios of 50/50 and 60/40. The dynamic cross-linking process resulted in a more stable EVA phase morphology with disperse and interconnected structures in the thermoplastic PA12 domains.     

Composition of Lipid Classes in the Morphologically Different Parts of the Olive Fruit,cv. Coratina (Olea europaea Linn.)

This paper reports on the distribution of “minor components” of olive oil in the four parts of olive fruit. The minor components are the nonglyceridic classes of compounds comprising vegetable oils. It was found that the classes and amounts of minor components varied to a great extent in the lipid fractions from skin, pulp, wood shell and seed. For 100 g of olives the weights of material comprising the minor components extracted by chloroform-ethanol (95:5) were the following: 103 mg in the skin, 200 mg in the pulp, 30 mg in the wood shells and 80 mg in the seeds. When the minor components were chromatographed by column chromatography or on thin-layer plates, the following classes of compounds were separated: alkanes, alkyl esters, methyl phenyl esters, steryl esters, aldehydes, alcohols, sterols, cyclic triterpenoids and very long chain free fatty acids.     

On the acidity of liquid and solid acid catalysts: Part 1. A thermodynamic point of view

The protonation equilibria of weak bases (B) in solid acids (HClO₄/SiO₂, CF₃SO₃H/SiO₂, H₂SO₄/SiO₂) were studied by UV spectroscopy and the results were compared to those obtained for analogous compounds in concentrated aqueous solutions of strong acids (HClO₄, CF₃SO₃H, H₂SO₄). The behaviour of B in liquid (L) and solid (S) phase was analysed by titration curves, log[BH⁺]/[B] ratios and thermodynamic pK _BH+ values. It has been shown that the proton transfer process acid → base (i.e., from (H+A^-)_(L,S) to (BH+A^-)_(L,S)} can be described by the relationship observed between the activity coefficient terms that are to be taken into account for acid–base equilibria occurring in nonideal systems ( – log(f _B f _B+/f _BH+)_(L,S)= -n _BA log(f _A–f _H+/f _HA)_(L,S)) and can be estimated by the n _BA values. Two “activity coefficient functions” (i.e., Mc(B) = – log(f _B f _B+/f _BH+)and Mc(s) = – log(f _A–f _H+/f _HA)) were used to describe, respectively, the equilibria of B and the equilibria of the acids in concentrated aqueous solutions and the meaning of terms “activity coefficient function” and “protonating ability of an acid” were discussed. The difference between “acidity functions”, determined for solutes (Ac(i)) and solvents (Ac(s)) in aqueous acids, and the H_x acidity functions, the latter developed for solutes in analogous media by the Hammett procedure, was also shown. This revised version was published online in July 2006 with corrections to the Cover Date.     

Improving minimum quantity lubrication in CBN grinding using compressed air wheel cleaning

The application of minimum quantity lubrication (MQL) in grinding has emerged as an alternative for reducing the abundant flow of cutting fluids, thus achieving cleaner production. Although considered an innovative technique in grinding operations, its widespread application is hindered due primarily to the high heat generation and wheel pore clogging caused by machined chips, harming the final product quality and increasing tool wear on the machine. This study sought to improve MQL use in grinding. In addition to the conventional MQL injected at the wheel/workpiece interface, a compressed air jet was used to clean the mixture of MQL oil and machined chips from clogged wheel pores. Experiments were conducted using external cylindrical plunge grinding on AISI 4340 quenched and tempered steel, and a vitrified cubic boron nitrite (CBN) wheel. The cooling-lubrication methods employed were the conventional flood coolant application, MQL (without cleaning), and MQL with a cleaning jet directed at the wheel surface at different angles of incidence. The main goal of these experiments was to verify the viability of replacing the traditional abundant flow of cutting fluid with MQL and wheel cleaning. The analyses were conducted by measuring the following output variables of the process: workpiece surface roughness and roundness errors, diametrical wheel wear, acoustic emission generated by the process, and metallographic images of the ground surface and subsurface. Results show the positive effects of implementing the cleaning jet technique as a technological improvement of minimum quantity lubrication in grinding in order to reduce the usage of cutting fluids. The MQL technique with cleaning compressed air jet, for a specific angle of incidence (30°), proved to be extremely efficient in the improvement of the surface quality and accurate workpiece shape; it also reduced wheel wear when compared to the other cooling-lubrication methods that were tested (without a cleaning jet).     

Spot counting to locate fetal cells in maternal blood and tissue: a comparison of manual and automated microscopy

BACKGROUND: Fetal cell detection in maternal tissue requires an accurate, efficient, and reproducible microscopy method. Our objective was to compare manual scoring to a commercially available automated scanning system for the detection of chromosome signals by fluorescence in situ hybridization (FISH). METHODS: X and Y chromosome FISH signals were detected on slides of calibrated mixtures of blood, paraffin-embedded liver sections, and post-termination blood. For manual scoring (400x magnification), the number of cells located and duration of scoring were recorded. For automated scanning using the Metasystems Metafer3/Metafer4 Scanning System (200x magnification), duration of scanning, number of gallery images generated, duration of manual review of gallery images, and number of confirmed fetal cells were recorded. RESULTS: From all slides the number of target fetal cells located by manual and automated microscopy was highly correlated (r = 0.90). However, automated scanning required on average 4-fold more time than manual scoring (P < 0.0001), with an average automated scanning time of 9.7 h per slide compared with 2.4 h per slide when scored manually. CONCLUSIONS: In general, the accuracy of automated and manual microscopy is comparable, although manual scoring is more efficient because of the level of magnification necessary for automated scanning of cells, and a large number of gallery images generated by automated scanning that must then be reviewed manually. This suggests that when rapid analysis is required (i.e., clinical situations), manual microscopy is preferable. In contrast, automated scanning may have advantages over manual microscopy when time constraints are less imposed (i.e., research situations).     

Accessory oculomotor nuclei of man. III. The nuclear complex of the posterior commissure: a Nissl and Golgi study

Galactocerebrosidase (GALC, EC 3.2.1.46) was purified from human urine by a series of hydrophobic affinity column chromatography steps. The activity was enriched 176,000-fold from concentrated urine by only four columns, including octyl Sepharose, hydroxylapatite, butyl Sepharose and ethyl-agarose. The overall recovery was about 20% but only low amounts were obtained due to its low abundance. The estimated final specific activities of several batches were between 1 and 2 mmol/h per mg protein. The final purified fractions were essentially free of other lysosomal enzyme activities. The most pure fractions showed a series of bands between 50 and 53 kDa on sodium dodecylsulfate-polyacrylamide gel electrophoresis which were determined to have identical N-terminal amino acid sequence. In addition, gel filtration of partially purified GALC after disassociation showed one peak of activity estimated to have a molecular mass near 50 kDa. GALC was also purified from human brain and human placenta using the same methods demonstrating the usefulness of this procedure in obtaining GALC from solid human tissues. In addition to the bands migrating near 50 kDa from urine, there were also bands at 80 kDa and 30 kDa in some preparations. By N-terminal sequencing and the use of antipeptide antibodies, the 80 kDa band was demonstrated to have the same N-terminal amino acids as the 50-53 kDa bands. The 30 kDa band had a unique sequence. The relationship between the different molecular weight species remains to be determined. The purification of GALC and the securing of amino acid sequence information will aid in the cloning of the GALC gene. This enzyme is deficient in human patients with Krabbe disease and several animal species.     

Haemophilus somnus immunoglobulin binding proteins and surface fibrils

The high-molecular-weight (HMW) immunoglobulin binding proteins (IgBPs) of Haemophilus somnus and a 76-kDa surface protein (p76) are found in serum-resistant virulent strains but not in several serum-sensitive strains from asymptomatic carriers. For the first time, p76 was shown to be an IgBP also. This was done by competitive inhibition studies with affinity-purified antidinitrophenol (anti-DNP) and DNP to ensure that binding was not antigen specific. The HMW IgBPs, but not the p76 IgBP, were partially purified from concentrated culture supernatant in detergent by fluid-phase liquid chromatography with a gel filtration column. Membrane extraction studies showed that p76 predominated in the Sarkosyl-soluble fraction of the bacterial cell pellet. Since integral outer membrane (OM) proteins are Sarkosyl insoluble, this is consistent with our previous finding that implicated p76 as a peripheral OM protein. The HMW IgBPs were found predominantly in the Sarkosyl-soluble fraction of the culture supernatant. This suggests that they were not integral membrane proteins and that their presence in the supernatant was not due to OM blebbing. We then showed that two IgBP-positive serum-resistant virulent strains have a surface fibrillar network but that two IgBP-negative serum-sensitive H. somnus strains from asymptomatic preputial carriers do not. Fibrils on the surfaces of IgBP+ strains bound gold-labelled bovine immunoglobulin G2 (IgG2) anti-DNP, indicating that these fibrils have IgG2 binding activity. Therefore, this study shows that H. somnus has two IgBPs, including a peripheral membrane protein and a fibrillar surface network.     

Nonphosphorylated peptide ligands for the Grb2 Src homology 2 domain

Critical intracellular signals in normal and malignant cells are transmitted by the adaptor protein Grb2 by means of its Src homology 2 (SH2) domain, which binds to phosphotyrosyl (pTyr) residues generated by the activation of tyrosine kinases. To understand this important control point and to design inhibitors, previous investigations have focused on the molecular mechanisms by which the Grb2 SH2 domain selectively binds pTyr containing peptides. In the current study, we demonstrate that the Grb2 SH2 domain can also bind in a pTyr independent manner. Using phage display, an 11-amino acid cyclic peptide, G1, has been identified that binds to the Grb2 SH2 domain but not the src SH2 domain. Synthetic G1 peptide blocks Grb2 SH2 domain association (IC50 10-25 microM) with a 9-amino acid pTyr-containing peptide derived from the SHC protein (pTyr317). These data and amino acid substitution analysis indicate that G1 interacts in the phosphopeptide binding site. G1 peptide requires a YXN sequence similar to that found in natural pTyr-containing ligands, and phosphorylation of the tyrosine increases G1 inhibitory activity. G1 also requires an internal disulfide bond to maintain the active binding conformation. Since the G1 peptide does not contain pTyr, it defines a new type of SH2 domain binding motif that may advance the design of Grb2 antagonists.