Recent progress toward a manufacturable polycrystalline SiC surface micromachining technology

In this paper, we present results of recent research from our laboratory directed toward a manufacturable SiC surface micromachining technology for microelectromechanical systems (MEMS) applications. These include the development of a low-pressure chemical vapor deposition and in situ doping processes for silicon carbide (SiC) films at relatively low temperatures, as well as the development of selective dry etching processes for SiC using nonmetallic masking materials. Doped polycrystalline SiC films are deposited at 800/spl deg/C by using a precursor 1,3-disilabutane and dopant gas NH/sub 3/, with the minimum resistivity of 26 m/spl Omega//spl middot/cm. Dry etching for SiC and its selectivity toward silicon dioxide and silicon nitride masking materials are investigated using SF/sub 6//O/sub 2/, HBr, and HBr/Cl/sub 2/ transformer coupled plasmas. The etch rate, etch selectivity, and etch profile are characterized and compared for each etch chemistry. By combining the LPCVD and dry etching process with conventional microfabrication technologies, a multiuser SiC MEMS process is developed.     

Evaluation of real-time PCR assays for detection and quantification of fraudulent addition of bovine milk to caprine and ovine milk for cheese manufacture

Many typical Italian cheeses made from ovine milk are certified as Protected Designation of Origin (PDO). Because caprine and ovine milk production is limited, the fraudulent addition of cows' milk is widespread. In addition, some compounds in bovine milk have high allergenic potential; therefore, such fraud also has implications for consumer health. In this study, a real-time polymerase chain reaction (real-time PCR) test was developed to detect and quantify cow's milk in caprine and ovine cheeses, based on two target genes. The mitochondrial Cytochrome b gene (Cytb) of Bos taurus was used to detect and quantify bovine DNA. The nuclear gene myostatin (Myo), nuclear ribosomal gene 18S, or mitochondrial gene 16S were used alternatively as universal reference markers. Caprine (n = 30) and ovine (n = 51) cheese samples were purchased and analyzed and most were shown to be contaminated by bovine milk. Pairwise analysis of quantification data using a Spearmann Rank Correlation test demonstrated a highly significant correlation between data obtained with the different reference assays.     

Application of immunoelectron microscopy techniques in the diagnosis of phytoplasma diseases

An immunoelectron microscopy technique was applied to label Chrysanthemum leuchanthemum phytoplasma in infected leaf tissues of Chrysanthemum leuchanthemum L. and Catharanthus roseus L. plants. Specific monoclonal antibodies at different dilutions and secondary antimouse antibody conjugated with colloidal gold particles of different sizes were used. The monoclonal antibodies demonstrated their specificity against the antigen; immunocytological methods permitted the precise localization and identification of phytoplasmas in thin sections from infected tissues.     

Ultrasmooth gold thin films by self-limiting galvanic displacement on silicon

Galvanic displacement (GD), a type of electroless deposition, has been used to obtain ultrasmooth gold thin films on silicon <111>. The novel aspect of the method presented herein is the absence of fluoride ions in the liquid phase, and its principal advantage when compared to previous efforts is that the process is inherently self-limiting. The self-limiting factor is due to the fact that in the absence of fluorinated species, no silicon oxide is removed during the process. Thus, the maximum gold film thickness is achieved when elemental silicon is no longer available once the surface is oxidized completely during the galvanic displacement process. X-ray photoelectron spectroscopy has been used as a tool for thickness measurement, using the gold to silicon ratio as an analytical signal. Three gold plating solutions with different concentrations of KAuCl? (2, 0.2, and 0.02 mM) have been used to obtain information about the formation rate of the gold film. This XPS analysis demonstrates the formation of gold films to a maximum thickness of ～3.5 ?. Atomic force microscopy is used to confirm surface smoothness, suggesting that the monolayer growth does not follow the Volmer-Weber growth mode, in contrast to the GD process from aqueous conditions with fluorinated species.     

Recovery of Staphylococcus aureus in Gray Mugil cephalus Roe (Bottarga): Investigation by an Integrated Cultural/Molecular Approach

In the Mediterranean area, salted and dried roe from the gray Mugil cephalus “bottarga” represent a speciality food with great commercial value. Bottarga is currently produced by a traditional handmade process and, the risk of human bacterial contamination during its manufacturing is still unknown; in this perspective the foodborne pathogen Staphylococcus aureus could potentially contaminate this product due to poor sanitation or bad handling during processing. The aim of this work is: to evaluate the contamination level of foodborne pathogens at different product manufacturing stages and, in addition, to describe a fast and realizable method for the rapid detection of S. aureus in bottarga samples in the field. A cultural procedure was initially used to investigate the occurrence of S. aureus and the other main foodborne pathogens in bottarga samples at the different manufacturing stages (from roe to final product). In addition, a molecular approach was used to rapidly determine the presence of total bacteria, S. aureus, and its potential toxigenicity. Of the 194 specimens analyzed, we identified: Clostridium perfringens, Enterococcus spp. and Enterobacteriaceae. However, some samples resulted as being contaminated with S. aureus (4% in roe and 8.7% in the final product). During the bottarga manufacturing process, we observed an increase in pathogen levels (from 10² to 10⁵ CFU/g) in contaminated samples, and entA and entB genotypes were identified. Reconstruction experiments suggest that the fresh roe and the bottarga (not completely dried) could represent a risk for the contamination and growth of pathogen bacteria.     

Heterogeneous nature of microalbuminuria in NIDDM: studies of endothelial function and renal structure

Glutathione-related enzymes are thought to influence the prognoses of patients with adenocarcinoma of the lung. In this study, the localization of these enzymes was examined immunohistochemically in the primary lesions and metastatic lymph nodes of 61 patients with primary adenocarcinoma. Strong immunoreactivity for glutathione peroxidase (GSH-PO) and superoxide dismutase (SOD) was found in tissue from patients with poor prognoses, while tissue from patients with favorable prognoses demonstrated only immunoreactivity for these enzymes. Therefore, believe that glutathione-related enzymes may serve as predictors of tumor resistance in patients with adenocarcinoma, hence measuring these enzymes may be useful in determining the need for adjuvant therapy.     

Liquefaction Resistance of Clean and Nonplastic Silty Sands Based on Cone Penetration Resistance

Liquefaction of granular soil deposits is one of the major causes of loss resulting from earthquakes. The accuracy in the assessment of the likelihood of liquefaction at a site affects the safety and economy of the design. In this paper, curves of cyclic resistance ratio (CRR) versus cone penetration test (CPT) stress-normalized cone resistance qc1 are developed from a combination of analysis and laboratory testing. The approach consists of two steps: (1) determination of the CRR as a function of relative density from cyclic triaxial tests performed on samples isotropically consolidated to 100 kPa; and (2) estimation of the stress-normalized cone resistance qc1 for the relative densities at which the soil liquefaction tests were performed. A well-tested penetration resistance analysis based on cavity expansion analysis was used to calculate qc1 for the various soil densities. A set of 64 cyclic triaxial tests were performed on specimens of Ottawa sand with nonplastic silt content in the range of 0–15% by weight, and relative densities from loose to dense for each gradation, to establish the relationship of the CRR to the soil state and fines content. The resulting (CRR)7.5-qc1 relationship for clean sand is consistent with widely accepted empirical relationships. The (CRR)7.5-qc1 relationships for the silty sands depend on the relative effect of silt content on the CRR and qc1. It is shown that the cone resistance increases at a higher rate with increasing silt content than does liquefaction resistance, shifting the (CRR)7.5-qc1 curves to the right. The (CRR)7.5-qc1 curves proposed for both clean and silty sands are consistent with field observations.     

Tribological Challenges in Micromechanical Systems

Despite much progress in surface micromachining technology, adhesion, friction and wear remain key issues, severely limiting the realization and reliability of many microelectromechanical systems (MEMS) devices. In this article, we focus on the use of molecularly thin organic films as release and anti-stiction coatings for MEMS. The various classes of organic films explored for MEMS are reviewed here, followed by a discussion of the current limitations and areas for improvements for this coating technology.     

N-[2-Methyl-5-(triazol-1-yl)phenyl]pyrimidin-2-amine as a scaffold for the synthesis of inhibitors of Bcr-Abl

N‐[2‐Methyl‐5‐(triazol‐1‐yl)phenyl]pyrimidin‐2‐amine derivatives were synthesized and evaluated in vitro for their potential use as inhibitors of Bcr‐Abl. The design is based on the bioisosterism between the 1,2,3‐triazole ring and the amide group. The synthesis involves a copper(I)‐catalyzed azide–alkyne cycloaddition (CuAAC) as the key step, with the exclusive production of anti‐(1,4)‐triazole derivatives. One of the compounds obtained shows general activity similar to that of imatinib; in particular, it was observed to be more effective in decreasing the fundamental function of cdc25A phosphatases in the K‐562 cell line.