A technique for studying the catalytic activity of enzymes spread as a film at an air-water interface, by exchanging the subphase under the film to remove unspread enzyme molecules, was developed, and its effectiveness was studied using surface-spread mitochondrial malate dehydrogenase. Mitochondrial malate dehydrogenase formed stable films which gave reproducible pi-A curves. The enzyme activity was measured by the oxidation rate of reduced nicotinamide adenine dinucleotide (NADH) in the presence of the substrate oxalacetic acid. Oxalacetic acid and NADH were injected into the subphase. The catalytic activity of the enzyme was dependent on the surface pressure of the film. The maximum catalytic activity was observed at a surface pressure of 4.4 dynes/cm. The activity was higher at intermediate surface pressures than at very low or very high surface pressures. A high bulk catalytic activity was observed in the unstable region, i.e., at a high degree of compression, of the film. The catalytic activity of the surface-spread enzyme was only a fraction of an equivalent amount of enzyme in solution. 相似文献
Pancreases from Syrian golden hamsters treated with N-nitrosobis(2-hydroxy-proply)amine for 10 to 25 weeks were examined under scanning electron microscopy (SEM). Findings indicate that the neoplasms originated from the ductal epithelium and developed progressively. Adenomas were lined by epithelium of differing cells types, ranging from a flat singly ciliated form to cuboidal-columnar types, or to mixed cell populations. The epithelial lining of the ductal carcinomas exhibited tubular and papillary cystic spaces, and cell surfaces were similar to the cuboidal and columnar epithelium of adenomas and of ductal epithelial hyperplasia. However, microvilli were dense and of varied lengths. The SEM observations correlated with patterns seen in routine histologic preparations. 相似文献
The lubrication of lightly loaded cylinders with rotating and normal motion was investigated using a non-newtonian power law fluid under cavitation boundary conditions. The load capacity and frictional drag increase as the squeeze velocity increases. This increase is enhanced by an increase in the flow behaviour index of the lubricant. However, when the two surfaces move apart these results are reversed. The effect of the flow behaviour index on the location of the point of cavitation does not seem to be very significant. 相似文献
The micropolar fluid theory-a possible non-Newtonian model for fluids with additives in which the average molecular size may be comparable to a characteristic material length scale—is applied to the problem of lubrication of a lightly loaded roller on a plane in combined sliding, rolling and normal motion. Three new parameters N, L and q are defined. Various bearing characteristics are presented graphically. It is shown that these three dimensionless parameters are of great significance in discussing the flow behavior of a lubricant with additives. The results of Dowson et al.[2] can be obtained as a particular case of the present study. The overall conclusion of the study, in conformity with the experimental results, is an increase in effective viscosity, resulting in an increase in the load carrying capacity and a decrease in coefficient of friction when additives are used. 相似文献
The objective of this study was to investigate the influence of two proteins, albumin and type B gelatin, on the physical aging of EUDRAGIT® RS 30 D and RL 30 D coated theophylline pellets. The physicomechanical properties of sprayed films, thermal properties of cast films, influence of proteins on the zeta potential and particle size of the dispersion, and the release of proteins from cast films under simulated dissolution conditions were investigated. The release rate of theophylline decreased significantly over time from pellets coated with an acrylic dispersion containing 10% albumin when there was no acidification of the acrylic dispersion; however, when pellets were coated with an acidified EUDRAGIT®/albumin dispersion, the theophylline release rate was stable for dosage forms stored in the absence of humidity. The drug release rate was faster for pellets coated with acrylic dispersions containing 10% gelatin compared to the albumin-containing formulations. When sprayed films were stored at 40°C/75% RH, the water vapor permeability decreased significantly for both EUDRAGIT® films and those containing EUDRAGIT® and albumin; however, there was no significant change in this parameter when 10% gelatin was present. Albumin was released from the acrylic films when the pH of the dissolution media was below the isoelectric point of the protein while no quantitative release of gelatin was observed in pH 1.2 or 7.4 media. The effect of gelatin to prevent the decrease in drug release rate was due to stabilization in water vapor permeability of the film. Acidification of the polymeric dispersion resulted in electrostatic repulsive forces between albumin and the acrylic polymer, which stabilized the drug release rate when the dosage forms were stored in aluminum induction sealed containers at both 40°C/75% RH and 25°C/60% RH. 相似文献
After earlier promising results for the control of anthracnose in avocado fruit by thyme vapours, our studies were extended to commercial use in tray packs. The effect of thyme oil low-density polyethylene impregnated pellets (TO-LDPE-P) in polylactic acid (PLA) sachets was investigated for the control of anthracnose and retention of dietary phytochemicals, fatty acid composition, D-mannoheptulose sugar and fruit quality in ready-to-eat avocado fruit. The 10% TO-LDPE-P significantly reduced the incidence severity of anthracnose and enabled the retention of dietary phytochemicals (p-coumaric, ferulic and caffeic acid, catechin and epicatechin), fatty acids, mannoheptulose, fruit firmness and taste compared to the currently used prochloraz® fungicide treatment. The results of this study strongly suggest the incorporation of 10% TO-LDPE-P in PLA sachets in commercial avocado tray packs as a natural option to improve fruit health, dietary phytochemicals, fatty acid composition and consumer satisfaction. 相似文献
The variation in viscosity with temperature is important in lubrication: this was studied by using a viscosity—film thickness relation. This is justified since it has been verified experimentally that the highest temperature occurs in zones where the film thickness is lowest. The use of additives was considered by using a micropolar fluid model. The geometry considered was that of an infinitely long journal bearing considering cavitation. There is an increase in the load capacity when additives are used. It was established theoretically that the use of additives increases the operating temperature range in bearings. 相似文献
Wireless Personal Communications - Joint employment of multi-hop data forwarding and mobile data-collector is a popular technique for efficient data collection in energy constraint and delay... 相似文献
This survey presents a brief discussion of different aspects of digital image watermarking. Included in the present discussion are these general concepts: major characteristics of digital watermark, novel and recent applications of watermarking, different kinds of watermarking techniques and common watermark embedding and extraction process. In addition, recent state-of-art watermarking techniques, potential issues and available solutions are discussed in brief. Further, the performance summary of the various state-of-art watermarking techniques is presented in tabular format. This survey contribution will be useful for the researchers to implement efficient watermarking techniques for secure e-governance applications.
Quantum teleportation of an unknown quantum state is one of the few communication tasks which has no classical counterpart. Usually the aim of teleportation is to send an unknown quantum state to a receiver. But is it possible in some way that the receiver’s state has more quantum discord than the sender’s state? We look at a scenario where Alice and Bob share a pure quantum state and Alice has an unknown quantum state. She performs joint measurement on her qubits and channel to prepare Bob’s qubits in a mixed state which has higher quantum discord than hers. We also observe an interesting feature in this scenario, when the quantum discord of Alice’s qubits increases, then the quantum discord of Bob’s prepared qubits decreases. Furthermore, we show that the fidelity of one-qubit quantum teleportation using Bob’s prepared qubits as the channel is higher than using Alice’s qubits. 相似文献