Antimicrobial Activity of Peptide P34 During Thermal Processing

In the present work, the influence of pH and sodium chloride concentration on thermal stability of antimicrobial peptide P34 was evaluated under different time–temperature conditions by a 2² factorial design experiment. At sterilization conditions (121 °C for 20 min), maximum retention (36%) was obtained at pH between 5.5 and 8.5 and sodium chloride concentration between 0.4 and 0.75 mol/l. For boiling conditions (100 °C for 20 min), antimicrobial activity was about 100% combining pH between 6.0 and 8.0 and salt concentration in the range of 0.65 to 1 mol/l. At low temperature pasteurization conditions (30 min at 65 °C), antimicrobial activity was not affected within the pH range from 5.0 to 8.0. For the three time–temperature conditions tested, the antimicrobial activity was minimal at more acidic or alkaline pH. Sodium chloride concentration of 0.65 mol/l increased thermostability of the peptide P34. Combination of sodium chloride and slight alkaline pH may increase the stability of peptide P34, which is essential to the proper utilization of bacteriocins in food industry.     

Differentiation of post harvest date fruit varieties non‐destructively using FT‐NIR spectroscopy

The world production of dates has more than doubled over the last two decades. Presently, the industrial sorting of dates is a difficult operation, affected by a high level of losses. As a consequence, the objective of this article is to study the variety discrimination capabilities of Fourier Transform‐Near Infrared (FT‐NIR) reflectance spectroscopy for post‐harvest sorting purposes. The same methodology was tested on six commercial date varieties named Estamaran, Kabkab, Mazafati, Piarom, Deglet nour and Zahedi. For each cultivar, 100 fruits were analysed using the FT‐NIR spectrometer, subsequently characterised by destructive measurements. A multivariate analysis of the FT‐NIR spectra appears a feasible tool for cultivar discrimination and even to estimate the soluble solid content (SSC) and dry matter (DM) of dates at their optimal harvesting conditions. FT‐NIR, as a non‐destructive techniques, could be a suitable way for on‐line screening of different dates varieties.     

De‐hairing activity of extracellular proteases produced by keratinolytic bacteria

The depilatory activity of keratinolytic bacteria was investigated. Three keratinolytic bacteria isolated from feather waste were tested for their ability to grow in mineral medium containing bovine hair as sole carbon, nitrogen and energy source. The strains kr2, kr6 and kr10 grew well on bovine hair, producing proteolytic activity as judged by assay with azokeratin as substrate. The proteolytic enzymes from all three strains showed similar pH and temperature optima. Strain kr2 showed mainly serine‐type protease activity, whereas kr6 and kr10 enzymes showed metalloprotease characteristics. The culture supernatants were used as crude enzyme preparations for depilatory studies on bovine pelts. All three strains were efficacious in promoting de‐hairing. Microscopic analysis revealed that the epidermis was completely removed and the absence of hair in follicles was observed. Proteases of keratinolytic bacteria were efficient in promoting depilation of bovine pelts. Enzymatic de‐hairing represents a suitable alternative to the conventional tannery process, with the benefit of avoiding environmental pollution. Copyright © 2003 Society of Chemical Industry     

Recent Advances in Food-Packing,Pharmaceutical and Biomedical Applications of Zein and Zein-Based Materials

Zein is a biodegradable and biocompatible material extracted from renewable resources; it comprises almost 80% of the whole protein content in corn. This review highlights and describes some zein and zein-based materials, focusing on biomedical applications. It was demonstrated in this review that the biodegradation and biocompatibility of zein are key parameters for its uses in the food-packing, biomedical and pharmaceutical fields. Furthermore, it was pointed out that the presence of hydrophilic-hydrophobic groups in zein chains is a very important aspect for obtaining material with different hydrophobicities by mixing with other moieties (polymeric or not), but also for obtaining derivatives with different properties. The physical and chemical characteristics and special structure (at the molecular, nano and micro scales) make zein molecules inherently superior to many other polymers from natural sources and synthetic ones. The film-forming property of zein and zein-based materials is important for several applications. The good electrospinnability of zein is important for producing zein and zein-based nanofibers for applications in tissue engineering and drug delivery. The use of zein’s hydrolysate peptides for reducing blood pressure is another important issue related to the application of derivatives of zein in the biomedical field. It is pointed out that the biodegradability and biocompatibility of zein and other inherent properties associated with zein’s structure allow a myriad of applications of such materials with great potential in the near future.     

Prediction of condensation in steam ejector for a refrigeration system

An experimental refrigeration system based on a two-stage steam ejector was set-up in the Thermodynamics and Heat Transfer Laboratory of our Department. The system optimization and realization have been described elsewhere ( [Grazzini and Mariani, 1998] and [Grazzini and Rocchetti, 2008] ). In both stages, primary flows are highly supersonic and reach low pressure and temperature levels. As usual in the literature, an ideal gas model was used during the design process. This paper is intended to check the validity of this assumption. In order to understand the actual working condition of our system, several models have been compared. The presence of high flow speed suggests the existence of metastable conditions. To set the border for the metastable region, the spinodal curve has been drawn. Isentropic expansion of vapour through the nozzle, modelled as ideal gas, seems well within the metastable zone. However, the Classic Nucleation Theory shows that the Wilson line is crossed at the nozzle throat. Condensation produces a marked difference in the conditions at the nozzle exit. Results coming from the present analysis will be used in further optimization of the experimental ejector design.     

Hydrolysis of tannery wastes to protein meal for animal feedstuffs: A process and product evaluation

Hydrolysis of protein solid wastes from tanneries to yield protein meal for animal nutrition has been performed in a pilot assembly designed to process 400 kg h^?1 of disposal material under optimum energy consumption and product yield conditions. Evaluation of the significance of amino acid degradation caused by the process, in relation to the product cost and quality in animal nutrition, was the purpose. A nutrition bioassay was carried out to study the incidence of dietary substitution of the above protein meal for soybean meal on growth performance by early weaned piglets. The results show that degradation of serine, proline, arginine and threonine occurs during collagen hydrolysis, and that only the threonine concentration in the product falls below the dietary requirement. However, integration of protein meal diets with the necessary amino acid content may be accomplished cheaply. Compared with other vegetable and animal commercial protein meals, the cost of the product obtained from tanned hides appears competitive. Processing of other tannery disposal material is not recommended, due to the low protein content of the starting material.     

Discoloration process modeling by neural network

The photo-oxidation of acid orange 52 dye was performed in the presence of H₂O₂, utilizing UV light, aiming the discoloration process modeling and the process variable influence characterization. The discoloration process was modeled by the use of feedforward neural network. Each sample was characterized by five independent variables (dye concentration, pH, hydrogen peroxide volume, temperature and time of operation) and a dependent variable (absorbance). The neural model has also provided, through Garson Partition coefficients and the Pertubation method, the independent variable influence order determination. The results indicated that the time of operation was the predominant variable and reaction mean temperature was the lesser influent variable. The neural model obtained presented coefficients of correlation on the order 0.98, for sets of trainability, validation and testing, indicating the power of prediction of the model and its character of generalization.     

Nanorobotics control design: a collective behavior approach for medicine

The authors present a new approach using genetic algorithms, neural networks, and nanorobotics concepts applied to the problem of control design for nanoassembly automation and its application in medicine. As a practical approach to validate the proposed design, we have elaborated and simulated a virtual environment focused on control automation for nanorobotics teams that exhibit collective behavior. This collective behavior is a suitable way to perform a large range of tasks and positional assembly manipulation in a complex three-dimensional workspace. We emphasize the application of such techniques as a feasible approach for the investigation of nanorobotics system design in nanomedicine. Theoretical and practical analyses of control modeling is one important aspect that will enable rapid development in the emerging field of nanotechnology.     

Effect of nanovesicle-encapsulated nisin on growth of Listeria monocytogenes in milk

Commercial nisin was encapsulated in nanovesicles (mean diameter 140 nm) prepared from partially purified soy lecithin. Nisin-loaded liposomes and unencapsulated (free) nisin were initially tested in BHI medium and skim milk inoculated with Listeria monocytogenes and incubated for 48 h at 30 °C. At such abuse temperature conditions, free nisin showed better inhibitory than the liposomal counterparts. Subsequently, the effect of encapsulated or free nisin was evaluated in combination with refrigeration (7 ± 1 °C) in both whole (3.25% fat) and skim (0% fat) milk for up to 14 day. A decrease of 3–4 log cycles in L. monocytogenes counts was observed for free and encapsulated nisin at 0.5 mg/ml concentration. Liposome encapsulation of antimicrobial peptides may be important to overcome stability issues and interaction with food components. The utilization of nanovesicle-encapsulated nisin in combination with low temperatures appeared to be effective to control L. monocytogenes in milk, emphasizing the importance of hurdle technology to assure food safety.