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MS Oitzl ER de Kloet M Jo?ls W Schmid TJ Cole 《Canadian Metallurgical Quarterly》1997,9(11):2284-2296
Previous studies in rats using the Morris water maze suggested that the processing of spatial information is modulated by corticosteroid hormones through mineralocorticoid and glucocorticoid receptors in the hippocampus. Mineralocorticoid receptors appear to be involved in the modulation of explorative behaviour, while additional activation of glucocorticoid receptors facilitates the storage of information. In the present study we used the water maze task to examine spatial learning and memory in mice homozygous and heterozygous for a targeted disruption of the glucocorticoid receptor gene. Compared with wild-type controls, homozygous and heterozygous mice were impaired in the processing of spatial but not visual information. Homozygous mutants performed variably during training, without specific platform-directed search strategies. The spatial learning disability was partly compensated for by increased motor activity. The deficits were indicative of a dysfunction of glucocorticoid receptors as well as of mineralocorticoid receptors. Although the heterozygous mice performed similarly to wild-type mice with respect to latency to find the platform, their strategy was more similar to that of the homozygous mice. Glucocorticoid receptor-related long-term spatial memory was impaired. The increased behavioural reactivity of the heterozygous mice in the open field points to a more prominent mineralocorticoid receptor-mediated function. The findings indicate that (i) the glucocorticoid receptor is of critical importance for the control of spatial behavioural functions, and (ii) mineralocorticoid receptor-mediated effects on this behaviour require interaction with functional glucocorticoid receptors. Until the development of site-specific, inducible glucocorticoid receptor mutants, glucocorticoid receptor-knockout mice present the only animal model for the study of corticosteroid-mediated effects in the complete absence of a functional receptor. 相似文献
73.
A revised lithostratigraphical framework of the Early to "Middle" Cretaceous strata of the Central North Sea (UK sector) derived from the rigorous examination of more than 100 commercial boreholes is presented. previously published frameworks have tended toward oversimplification of the Early to "Middle" Cretaceous, as the interval was considered initially to be of minor importance with regard to prospectivity. However: there is a general feeling in the industry that the Early to "Middle" Cretaceous is prospective. particularly with regard to subtle stratigraphical traps like the Kilda/Bosun and Scapa fields. It is inevitable that as more attention, due to hydrocarbon exploration, is paid to an interval that the stratigraphical models used progress from the simplistic to the complex. The framework presented here is the result of a pragmatic approach in that it is based upon the integration of previously published schemes. with the erection of new units derived from new analyses and interpretation. The complete lithostratigraphical framework is defined. explained and discussed with reference to borehole examples in the Central North Sea . 相似文献
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In Exp 1, pigeons (Columba livia) were trained in a successive slide presentation procedure to discriminate between pictures of 2 ends of a room and then trained to find food in the actual room. A congruent-transfer (CT) group learned the spatial discrimination more quickly than an incongruent-transfer (IT) group. In Exp 2's replication we used a simultaneous slide presentation procedure and added a control group. The IT group required significantly more trials than the CT or control groups. In Exp 3, order of the training conditions was reversed. CT and IT conditions had no effect on the speed of acquisition of the discrimination. This indicates that pigeons acquire a representation of spatial locations from pictures, which can then direct behavior, but the direction of transfer observed was unidirectional. This suggests that a discrimination between spatial locations may not be accurately represented in pictorial form. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
77.
AA Artru 《Canadian Metallurgical Quarterly》1993,5(3):178-186
Propofol decreases intraocular pressure (IOP) and the IOP response to laryngoscopy and intubation, but the mechanisms responsible for this effect have not been reported. The present study examined the effect of propofol on IOP, intraocular fluid formation and outflow facility, and intraocular compliance. Twenty-two white New Zealand rabbits were anesthetized with halothane (0.8%-1.0% inspired concentration) in nitrous oxide (2 L/min) and oxygen (1 L/min). Muscle paralysis was established with pancuronium, and the lungs were mechanically ventilated through a tracheal tube. Twelve rabbits examined under these conditions served as controls. In the treatment group (n = 10), 6 mg/kg propofol followed by 18 mg.kg-1 x h-1 propofol intravenously was added to halothane/nitrous oxide/oxygen anesthesia. In both groups, a series of intraocular infusions was made via a 30-gauge needle in the anterior chamber, and IOP, the rate of aqueous humor formation (Fa), and trabecular outflow facility (Ctr) were determined using conventional analysis. These same measures, as well as intraocular compliance, were determined using a new method of analysis adapted from the manometric technique for determining cerebrospinal fluid dynamics. IOP was 11.3 +/- 1.8 mm Hg (mean +/- SD) in halothane-anesthetized controls and decreased to 9.4 +/- 2.2 mm Hg when propofol was added to halothane anesthesia (P < 0.05). By conventional analysis, Fa was 2.82 +/- 0.94 microL/min and Ctr was 0.121 +/- 0.044 microL.min-1 x mm Hg-1 in controls. After addition of propofol, Fa decreased by 24% to 2.15 +/- 0.62 microL/min (P < 0.05) and Ctr decreased by 18% to 0.099 +/- 0.034 microL.min-1 x mm Hg-1 (P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
78.
The proteolytic activity produced by a Bacillus subtilis isolated from a hot spring was investigated. Maximum protease production was obtained after 38 h of fermentation. Effects of various carbon and nitrogen sources indicate the requirement of starch and bacteriological peptone to be the best inducers for maximum protease production. Requirement for phosphorus was very evident, and the protease was secreted over a wide range of pH 5-11. The partially purified enzyme was stable at 60 degrees C for 30 min. Calcium ions were effective in stabilizing the enzyme, especially at higher temperature. The enzyme was extremely salt tolerant and retained 100% activity in 5M NaCl over 96 h. The molecular weight of the purified enzymes as determined by SDS-PAGE was 28,000. The enzyme was completely inactivated by PMSF, but little affected by urea, sodium dodecyl sulfate, and sodium tripoly phosphate. 相似文献
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