c-KIT expression enhances the leukemogenic potential of 32D cells

The growth of human leukemic cells in culture and in vivo is dependent upon the presence of hematopoietic growth factors. Most populations of human leukemic acute myeloblastic leukemia (AML) cells express c-Kit on their surface and respond to Kit ligand (KL) in culture. To determine if this interaction was of potential significance in vivo we used a mouse model system. 32D cells, a murine IL-3-dependent myeloid cell line, were rendered KL responsive by transfection of the murine c-Kit. After injection of 32D or 32D-Kit cells into syngeneic hosts, animals bearing 32D-Kit cells, but not 32D cells, became moribund and were killed. These animals had circulating leukemic blast cells, infiltration of bone marrow, spleen, brain, liver, lung, and kidney. Cells recovered from some of the animals continued to be dependent upon IL-3 or KL for growth while in other cases the cells were factor independent. This model illustrates that the constitutive expression of c-Kit enhances the leukemic potential of 32D cells. The model will be useful for studying the progression of leukemia in vivo and testing whether interruption of the interaction of Kit and KL can affect the growth of leukemic cells.     

Modified atmosphere packaging of a mixed prepared vegetable salad dish

The aim of this study was to design a modified atmosphere package for a mixed vegetable salad consisting of 75 g of cut carrot, 55 g of cut cucumber, 20 g of sliced garlic and 50 g of whole green pepper. Respiration data of all the components were combined with film permeability data to predict package atmospheres and design optimal packages for experimental testing for improved shelf-life of the produce. The optimal package avoided minimum O₂ and maximum CO₂ tolerance limits, and chilling injury temperatures for any component. A pouch form package made of 27 mm low density polyethylene developed a modified atmosphere of 2.0–2.1% O₂ and 5.5–5.7% CO₂, which was beneficial for all components and provided better quality retention than other test packages.     

Performance of advanced resuscitation skills by pediatric housestaff

OBJECTIVE: To describe pediatric housestaff resuscitation experience and their ability to perform key resuscitation skills. DESIGN: Cohort study of 63 pediatric residents in a university-based training program. PARTICIPANTS AND METHODS: Investigators observed, scored, and timed resident performance on 4 key resuscitation skills. Cognitive ability was tested with 4 written scenarios. Housestaff provided self-reports of the number of months since their last American Heart Association Pediatric Advanced Life Support course, number of mock and actual codes attended, number of times skills were performed, and self-confidence with respect to resuscitation. RESULTS: A total of 45 pediatric residents (71%) participated. Median cognitive score was 5 (range, 1-5). Of all residents, 44 (97%) successfully bag mask-ventilated the mannequin; 24 (53%) and 36 (80%) used the correct bag and mask size, respectively. Thirty-nine residents (87%) placed a tube in the mannequin trachea, 12 (27%) checked that suction was working prior to intubation, and 30 (67%) chose the correct endotracheal tube size. Forty residents (89%) discharged the defibrillator, and 25 (56%) and 32 (71%) correctly chose asynchronous mode and infant paddles, respectively. Thirty-eight residents (84%) inserted an intraosseous line; 35 (78%) had correct placement. Median times for successful skill completion were 83 seconds for bag mask ventilation, 136 seconds for intubation, 149 seconds for defibrillation, and 68 seconds for intraosseous line placement. CONCLUSION: Pediatric housestaff previously trained in pediatric advanced life support were generally able to reach the end point of 4 key resuscitation skills but less frequently performed the specific subcomponents of each skill. This poor performance and the prolonged time to skill completion suggest the need for greater attention to detail during training.     

基于局部差别的形态学边缘检测方法研究

图像边缘特征是图像最基本也是最重要的特征之一.图像边缘特征的提取一直是计算机视觉和图像处理领域的经典研究课题,在精确制导武器的制导图预处理中有着广泛地应用.针对经典的边缘检测算子不能很好地提取噪声图像边缘这一问题,在数学形态学的基础上,提出了一种边缘提取方法.利用局部差别准则确定图像中受噪声影响的像素点,并对其进行形态学操作,滤除噪声,保存边缘.实验表明,该算法不仅具有很好的边缘提取能力,还具有很强的抗噪能力.说明了该算法的有效性和实用性.     

TSP203超前地质预报仪在隧道施工中的应用

大风垭口隧道地质及水文条件极其复杂,TSP203超前地质预报仪作为最先进的地质预报仪在大风垭口隧道施工中发挥了积极的作用。简单介绍TSP203超前地质预报仪的使用方法及其特点,根据TSP203预报结果与实际施工进行验证,体现其预报的精确性和长距离预报的优越性,建议今后类似工程中推广应用TSP203超前地质预报仪。     

一种新型特级防火卷帘的研究与开发——3J特级防火卷帘

介绍3J特级防火卷帘的各种重要功能特点,讨论其实用性与易用性以及推广该产品的必要性与可行性。     

缺血性糖尿病下肢病变的膝下动脉成形术

目的探讨膝下动脉的球囊成形术在治疗糖尿病缺血性下肢病变中的价值。方法对５３例糖尿病合并缺血性下肢病变患者６４侧缺血性下肢进行膝下动脉Deep球囊扩张成形术。对比患者手术前后临床症状的改变及足部溃疡的变化。结果５３例患者共１１９支动脉分支接受PTA治疗，５０例患者的１０１个分支成功地开通，技术成功率为８４．９％，所有５０例患者的临床症状均明显缓解，足部溃疡均变小，无需截肢。结论膝下动脉的球囊成形术是治疗缺血性糖尿病下肢病变安全有效的微创手段     

赋予针织服装个性魅力的女红工艺

将富于变化的女红工艺与单调的针织服装相结合，会使针织服装由平淡变为丰富，由呆板变为活泼。文章论述了在针织服装中运用女红图案、色彩、材料、针法的原理。