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101.
The administration of recombinant erythropoietin (rHuEpo) to anemic chronic renal failure patients may be associated with an increase in blood pressure, possibly by direct effects on peripheral blood vessels. In the present study, experiments were designed to explore the hypothesis that rHuEpo could enhance vascular resistance through mitogenic effect on vascular smooth muscle cells (VSMCs), and that preexisting hypertension might be a predisposing condition. Cultured VSMCs from the thoracic aortae of spontaneously hypertensive (SHR) and normotensive Wistar-Kyoto (WKY) rats were studied for DNA synthesis, phospholipase C activity, and cell growth related proto-oncogene expression in the presence of rHuEpo. In cells from both strains, rHuEpo dose-dependently increased DNA synthesis and stimulated phospholipase C activity, as indicated by 3H-thymidine incorporation and 3H-inositol phosphate formation, respectively (EC50 approximately 4 U/ml). Exposure of VSMCs to rHuEpo for various times gradually increased the levels of c-myc and junB and transiently induced c-fos expression, as determined by Northern analysis. rHuEpo-induced DNA synthesis was markedly enhanced in VSMCs from SHR compared to those from WKY. In contrast, rHuEpo-induced phospholipase C activity and proto-oncogene expression did not differ between the two strains. Taken together, these results suggest that rHuEpo may function as a vascular smooth muscle cell growth promoting factor through activation of the phospholipase C cascade and modulation of proto-oncogene expression. It could thereby contribute to vascular hypertrophy and arterial hypertension. 相似文献
102.
Pressure to compress the development life cycle and reduce the duration and resources committed to testing lead to experimentation in testing at the NASA Goddard Space Flight Centerís Software Engineering Laboratory. This study investigates the trend to reduce developer testing and increasingly rely upon inspection techniques and independent functional testing to shorten the development life cycle, improve testing productivity, and improve software quality.An approach is developed to conduct this comparison. In particular, the problem faced by software researchers, having a comprehensive characterization of software projects so similar types may be identified for comparative studies, is addressed using expert opinion. 相似文献
103.
We have used in vivo balloon catheterization in combination with in vitro organ culture to develop a model system for vascular neointima formation. A Fogarty balloon catheter was used to deendothelialize and rupture the internal elastic lamina of aortae in adult rabbits. After three d of recovery, aortae were harvested, divided into segments, and placed into organ culture. We obtained a daily index of cell proliferation in cultured vessels using [3H]thymidine incorporation into DNA. Also, segments were collected and processed for routine histology or immunohistochemistry. Aortic segments that had undergone ballooning 3 d before harvest and then cultured exhibited diffuse neointimal growth after several d in vitro, whereas those from sham-operated (nonballooned) rabbits showed generally only a single endothelial cell layer that is characteristic of normal intima. Aortae that were harvested, balloon-damaged in vitro, and then cultured exhibited no neointimal growth. The neointima that developed in cultured segments from in vivo ballooned rabbits was primarily of smooth muscle cell origin as determined by positive immunostaining for alpha-smooth muscle actin. The intima:media thickness ratios were significantly higher in aortic segments from ballooned rabbits at harvest and after 4 or 7 d in culture compared with those from nonballooned rabbits. Also, the [3H]thymidine index was higher in the in vivo ballooned aorta compared to non-ballooned or in vitro ballooned vessel. We conclude that ballooning in vivo followed by exposure to blood-borne elements produces an enhanced proliferative response in cultured vessels that is distinct from other in vitro models of neointimal growth. 相似文献
104.
Children with sickle-cell anaemia are predisposed to thrombotic strokes, the aetiology of which is unclear. We propose that erythropoietin, produced in response to chronic anaemia, is responsible for changes in platelet reactivity with a resulting increase in thromboses. This hypothesis is based on reports of enhanced aggregability of erythropoietin-driven platelets and an increased rate of thrombosis in patients receiving large doses of recombinant erythropoietin. Experiments in animals have shown that erythropoietin stimulates synthesis of platelets, that erythropoietin-driven platelets are hyper-reactive compared with age-matched control platelets, and that erythropoietin is pro-thrombotic. These data suggest that erythropoietin-dependent changes in platelet reactivity may potentiate thrombosis in sickle-cell anaemia, particularly in children who, compared with adults, have markedly higher erythropoietin concentrations and incidence of strokes. 相似文献
105.
Dr. A. Klar 《Computing》1995,55(3):207-221
We consider the numerical computation of nonlinear functionals of distribution functions approximated by point measures. Two methods are described and estimates for the speed of convergence as the number of points tends to infinity are given. Moreover, numerical results for the entropy functional are presented. 相似文献
106.
Summary To understand some main ink flow mechanisms in a mimeograph printer, the continuum mechanics theory of fluid-saturated porous solids is applied to analyze the ink flow through screens and a printing master, which are modeled by a fluid-filled poroelastic single layer. The layer is assumed to be pressed by a press roller from below and to be subjected from above to the hydrodynamic lubrication pressure induced between a printing drum and an ink roller. Nonlinear elasticity of the layer and dependence of the permeability on its deformation are taken into account. The numerical results show some important results: e.g., squeezing-out of the ink from the layer by the layer compaction by the press roller plays an important role, especially for the case where the compression duration is small in comparison with the characteristic time of the ink-filled poroelastic layer. 相似文献
107.
Prof. Dr. S. Darwish Dr. M. W. Khalil Dr. M. A. Abdel Rahim Porf. Dr. I. A. Ammar 《Materialwissenschaft und Werkstofftechnik》1989,20(9):299-308
A study on the value metal character of Zr in 0.1 M solutions of H2SO4, HNO3, and H3PO4 has been performed using the anode potential as the primary variable in galvanostatic, potentiostatic, and capacity measurements. A method of surface pre-treatment, which suppresses both O2 evolution and metal dissolution, has been described. Kinetic parameters of oxide growth have been calculated. The results indicate that:
- (i) the high field approximation is applicable following an exponential law, and
- (ii) the height and activation distance of the energy barrier for ion transport through the oxide phase (Verwey model) are the same three acids.
108.
Dr. J. Awrejcewicz 《Acta Mechanica》1989,77(1-2):111-120
Summary The paper presents an analysis of the transition from regular to chaotic motion in a Van der Pol-Duffing's oscillator with delay after a Hopf bifurcation. The conditions for the occurrence of the Hopf bifurcation have been determined by means of the approximate method. For the parameters near the bifurcation point a computer simulation of the vibrating system had been performed and the evolution of the system from regular motion to chaos has been analysed at the decrease of the value of the dimensionless damping coefficient.With 2 Figures 相似文献
109.
Priv. Doz. Dr.-Ing. habil M. Piesche 《Acta Mechanica》1989,78(3-4):175-189
Summary The subject of the present theoretical and experimental work is laminar flow processes in the gap between a rotating disc and a stationary wall. A report is given of the creation of suitable computational methods for determining the pressure gradient and velocity field in the impeller-side space of specifically slow-working rotary pumps in the process of conveying very viscous media with Newton properties, given temperature-dependent viscosity. The exposition is based on the abstract model conception of parallel radial gaps, the resultant frictional torques and the axial forces being determined for the case of a superimposed throughflow. On the one hand, the basis of the computations is an implicit difference method, and on the other an asymptotic computational method which leads to success via disturbance estimates in conjunction with similarity transformations, and approaches the difference method in accuracy while being relatively simple to handle and requiring little expenditure of time. On the basis of the computational results, experiments are conducted using a suitable experimental set-up, which answer the question concerning the efficiency and area of validity of the computational model.Notation
c
p
specific heat capacity
-
f
ax
axial force
-
m
moment of rotation
-
p
pressure
-
q
throughflow parameter
-
r
radius
-
r, ,z
coordinates
-
s
gap width
-
u, v, w
velocities
-
volume flow
-
angular velocity
-
viscosity
-
thermal conductivity
-
density
-
material parameter
-
a
external
-
i
internal
-
w
wall
With 7 Figures 相似文献
110.
Ectomography, a general tomographic method currently being implemented in nuclear medicine, is described. The object is viewed from different directions from the same projection angle, and the reconstruction process can be described as a two-dimensional filtered back-projection technique. One limitation of this limited-angle approach is that a three-dimensional Fourier representation of the acquired projections has a cone where data are missing. This empty cone can cause distortions in the tomogram by insufficient elimination of details outside the reconstructed tomographic section and by distortion of details in the section. The degree of distortion is dependent on the extension of the object in all directions and on the projection angle. Despite these limitations ectomography can be superior to single-photon-emission computed tomography for imaging sections that are close and parallel to the surface of the object, as in imaging of the hip joint or the heart. 相似文献