Real imaging and size values of Saccharomyces cerevisiae cells with comparable contrast tuning to two environmental scanning electron microscopy modes

The combined application of electron microscopy (EM) is frequently used for the microstructural investigation of biological specimens and plays two important roles in the quantification and in gaining an improved understanding of biological phenomena by making use of the highest resolution capability provided by EM. The possibility of imaging wet specimens in their "native" states in the environmental scanning electron microscope (ESEM) at high resolution and large depth of focus in real time is discussed in this paper. It is demonstrated here that new features can be discovered by the elimination of even the least hazardous approaches in some preparation techniques, that destroy the samples. Since the analysis conditions may influence the morphology and the extreme surface sensitivity of living biological systems, the results obtained from the same cultured cell with two different ESEM modes (Lvac mode and wet mode) were compared. This offers new opportunities compared with ESEM-wet/Lvac-mode imaging, since wet-mode imaging involves a real contrast and gives an indication of the changes in cell morphology and structure required for cell viability. In this study, wet-mode imaging was optimized using the unique ability of cell quantities for microcharacterization in situ giving very fine features of topological effects. Accordingly, the progress is reported by comparing the results of these two modes, which demonstrate interesting application details. In general, the functional comparisons have revealed that the fresh unprocessed Saccharomyces cerevisiae cells (ESEM-wet mode) were essentially unaltered with improved and minimal specimen preparation timescales, and the optimal cell viability degree was visualized and also measured quantitatively while the cell size remained unchanged with continuous images.     

Comparison of the ozonation and Fenton process performances for the treatment of antibiotic containing manure

An integrated treatment method based on magnesium salt extraction followed by chemical oxidation was used for the treatment of a veterinary antibiotic, oxytetracycline (OTC) contaminated cow manure since animal manure can be an important source for antibiotic pollution in the environment. Pretreatment with magnesium salt enhanced the efficiencies of subsequent oxidation processes by extracting 63.9% of OTC from the manure thereby making it more favorable for oxidation with the hydroxyl radicals produced by the Fenton and ozone oxidation processes. Both the 24 h Fenton oxidation process with 434 mM H₂O₂ and 43.4 mM Fe²⁺ doses and the 1-h ozonation process with an applied ozone dose of 2.5 mg min^− 1 provided more than 90% OTC removal from the manure slurry. However, the second-order OTC removal rate constant of Fenton process (119 M^− 1s^− 1) was remarkably lower than that obtained with the ozonation process (548 M^− 1s^− 1). The oxidant dose was a significant factor for the efficiency of the Fenton treatment but not for the ozone treatment. The efficiencies of both the Fenton and ozone oxidation processes were not affected by the pH adjustment of the manure slurry.     

艾哈迈德·阿德南·萨伊贡艺术中心,伊兹密尔,土耳其

<正>一座建筑的性能应当如何来衡量?除了通过统治当代建筑舆论的美学、技术以及经济价值来进行描述之外,我们还能够如何来阐释一座建筑,尤其是一座致力于庇护那些渴望展现自己的艺术家的建筑?有人会问成功的表演是如何实现的,表演者是如何进行伪装的、又是如何展现自己的?根据海德格尔的理论,当我们看不到钉子的时候,铁锤的表现才是成功的;钉子本身的出现通常表明出现了问题。     

Effect of garlic powder on the performance,egg traits and blood parameters of laying hens

This study was conducted to investigate the effects of garlic powder on the performance, egg traits and blood parameters of laying hens. One hundred and sixty‐two SHSY‐type brown layers aged 21 weeks were chosen at random from a large flock. They were allocated to three dietary treatments. Each treatment comprised six replicates of nine layers in groups of three. The diets were supplemented with 0, 5 and 10 g kg^?1 garlic powder. The experimental period lasted 22 weeks. Garlic powder addition did not significantly affect body weight, egg production, feed consumption, feed efficiency, egg shell index, egg breaking strength, egg shell thickness, egg albumen index, egg yolk index, egg Haugh unit, egg yolk weight and serum protein concentration. Egg weight increased (P < 0.01) with garlic powder supplementation. There was a significant (P < 0.01) reduction in egg cholesterol concentration as mg g^?1 yolk when the dietary level of garlic powder was increased from 0 to 10 g kg^?1. Hen serum triglyceride (P < 0.05) and total cholesterol (P < 0.01) concentrations decreased with garlic powder supplementation. This study demonstrated that garlic powder addition increased egg weight and decreased egg yolk cholesterol concentration (mg g^?1 yolk) and serum triglyceride and cholesterol concentrations without adverse effects on performance and egg traits. Copyright © 2006 Society of Chemical Industry     

Second-Law Analysis for an Inclined Channel Containing Porous-Clear Fluid Layers by Using the Differential Transform Method

In this study, convection in a porous medium for a laminar, incompressible, non-Darcy model flow in an inclined channel has been investigated. The flow field considered is composed of porous and clear viscous layers. The solutions are carried out for both clear fluid and porous regions by using the differential transform method (DTM). For the solutions of governing equations, constant values for some parameters such as angle of inclination (φ), porous parameter (σ), and the ratio of the heights of two layers (h) are assigned. In order to verify the applied solution technique, the results obtained are compared to the already existing ones evaluated by perturbation method. It is noticed that the results by two methods are in agreement for small values of Brinkman number (Br). However, for higher values of Br, the solutions carried out by perturbation method lose accuracy but the results of the DTM are still valid. The entropy generation number (N _s ) is derived and plotted by using dimensionless velocity and temperature profiles. One of the advantages of this study to similar studies is to give an open form series solution, which gives a tractable and easily applicable recurative form of nonlinear field equations. In similar studies, it is said that the equations are solved; however, neither solution technique nor accuracy or applicability of given technique are clear. In this work, these are well documented.     

The role of coal in energy production—Consumption and sustainable development of Turkey

Energy is one of the indispensable factors regarding the assurance of social prosperity and economic development of a country. The developing countries struggle to obtain energy sources reliable in the long term to complete their economic development, and the developed countries struggle to get them to keep their present prosperity levels. Coal seems to continue its indispensable position among the other energy sources for many years because of its production in more than 50 countries, the least effect it has from the price fluctuation and its usage lifespan of more than 200 years. The countries that are aware of it have investments in mining fields in the countries rich in coal reserves, and they continue investing considerable amount of money.     

A new arc detection method based on fuzzy logic using S-transform for pantograph–catenary systems

pantograph–catenary system is one of the critical components used in electrical trains. It ensures the transmission of the electrical energy to the train taken from the substation that is required for electrical trains. The condition monitoring and early diagnosis for pantograph–catenary systems are very important in terms of rail transport disruption. In this study, a new method is proposed for arc detection in the pantograph–catenary system based signal processing and S-transform. Arc detection and condition monitoring were achieved by using current signals received from a real pantograph–catenary system. Firstly, model based current data for pantograph–catenary system is obtained from Mayr arc model. The method with S-transform is developed by using this current data. Noises on the current signal are eliminated by applying a low pass filter to the current signal. The peak values of the noiseless signals are determined by taking absolute values of these signals in a certain frequency range. After the data of the peak points has been normalized, a new signal will be obtained by combining these points via a linear interpolation method. The frequency-time analysis was realized by applying S-transform on the signal obtained from peak values. Feature extraction that obtained by S-matrix was used in the fuzzy system. The current signal is detected the contdition as healthy or faulty by using the outputs of the fuzzy system. Furthermore the real-time processing of the proposed method is examined by applying to the current signal received from a locomotive.     

A flexible and scalable audio information retrieval system for mixed‐type audio signals

The content‐based classification and retrieval of real‐world audio clips is one of the challenging tasks in multimedia information retrieval. Although the problem has been well studied in the last two decades, most of the current retrieval systems cannot provide flexible querying of audio clips due to the mixed‐type form (e.g., speech over music and speech over environmental sound) of audio information in real world. We present here a complete, scalable, and extensible content‐based classification and retrieval system for mixed‐type audio clips. The system gives users an opportunity for flexible querying of audio data semantically by providing four alternative ways, namely, querying by mixed‐type audio classes, querying by domain‐based fuzzy classes, querying by temporal information and temporal relationships, and querying by example (QBE). In order to reduce the retrieval time, a hash‐based indexing technique is introduced. Two kinds of experiments were conducted on the audio tracks of the TRECVID news broadcasts to evaluate the performance of the proposed system. The results obtained from our experiments demonstrate that the Audio Spectrum Flatness feature in MPEG‐7 standard performs better in music audio samples compared to other kinds of audio samples and the system is robust under different conditions. © 2011 Wiley Periodicals, Inc.