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排序方式: 共有567条查询结果,搜索用时 15 毫秒
41.
Rosalba Lanciotti Milena Sinigaglia Fausto Gardini Lucia Vannini Maria Elisabetta Guerzoni 《Food microbiology》2001,18(6):659
This study focuses its attention on the boundary between the growth and no growth of three strains ofSalmonella enteritidis , Bacillus cereus and Staphylococcus aureus in the presence of growth controlling factors such as temperature, pH, water activity (Aw) and ethanol concentration. Preliminarly, the minimal values of pH, Awand temperature, and the maximum ethanol concentrations allowing the growth of the considered micro-organisms were determined. The calculation of these values enabled the use of logistic model to evaluate the growth/no growth boundary for the bacteria in relation to the considered independent variables. The location of the growth/no-growth boundaries for S. enteritidis and Staph. aureus were strongly affected, at the same ethanol concentration, by temperature, pH and Aw. Among the considered species, Staph. aureus was endowed with the highest sensitivity to low pH values whileB. cereus's growth/no growth interface, was quite unaffected by the combination of the stresses, when the physico–chemical conditions were above the minimum for growth. The effects of temperature, Awand ethanol on the limitation of growth of the considered species were not merely additive. It was possible to identify the combinations of such factors preventing the growth of Salmonella enteritidis, Staph. aureus and B. cereus. 相似文献
42.
SVD-matching using SIFT features 总被引:10,自引:0,他引:10
43.
Falcieri E Burattini S Bortul R Luchetti F Tabellini G Tazzari PL Cappellini A Cocco L Martelli AM 《Microscopy research and technique》2003,62(3):192-200
Two distinct types of cell death have been described: apoptosis and necrosis. However, it is becoming increasingly clear that the differences between these two types are far less numerous than initially thought. Morphological analyses might provide important information to distinguish apoptotic from necrotic samples. We recently reported that in necrotic, but not apoptotic, HL-60 human myeloid leukaemia cells, the nuclear protein topoisomerase IIalpha concentrated in nucleoli. In order to ascertain whether or not this phenomenon was restricted to a peculiar cell type or could be detected also in cells of lymphoid lineage, we performed an investigation aimed at defining the localization of topoisomerase IIalpha in apoptotic and necrotic Jurkat human T lymphoblastoid cells. Immunofluorescence staining demonstrated that topoisomerase IIalpha was excluded from the condensed chromatin of apoptotic cells, whereas in necrotic cells it was localized in discrete nuclear dots. Immuno-electron microscopy analysis showed that topoisomerase IIalpha was undetectable in nucleoli of normal and apoptotic cells, whereas it was present in the nucleolus of necrotic cells irrespectively of the type of inducer used (ethanol, H(2)O(2), HgCl(2)). Taken together, our findings identify topoisomerase IIalpha as a potential morphological marker useful to discriminate between apoptotic and necrotic cells. 相似文献
44.
George Caridakis Amaryllis Raouzaiou Elisabetta Bevacqua Maurizio Mancini Kostas Karpouzis Lori Malatesta Catherine Pelachaud 《Language Resources and Evaluation》2007,41(3-4):367-388
This work is about multimodal and expressive synthesis on virtual agents, based on the analysis of actions performed by human
users. As input we consider the image sequence of the recorded human behavior. Computer vision and image processing techniques
are incorporated in order to detect cues needed for expressivity features extraction. The multimodality of the approach lies
in the fact that both facial and gestural aspects of the user’s behavior are analyzed and processed. The mimicry consists
of perception, interpretation, planning and animation of the expressions shown by the human, resulting not in an exact duplicate
rather than an expressive model of the user’s original behavior. 相似文献
45.
Theera Anukunprasert Chintana Saiwan Elisabetta Di Bartolomeo Enrico Traversa 《Journal of Electroceramics》2007,18(3-4):295-303
Nanostructured powders of Nb-doped TiO2 (TN) and SnO2 mixed with Nb-doped TiO2 in two different atomic ratios—10 to 1 (TSN 101) and 1 to 1 (TSN 11)—were synthesized using the reverse micelle microemulsion
of a nonionic surfactant (brine solution/1-hexanol/Triton X-100/cyclohexane). The powders were characterized by transmission
electron microscopy (TEM) and X-ray diffraction (XRD). Thick films were fabricated for gas sensors and characterized by XRD
analysis and field emission scanning electron microscopy (FE-SEM). The effects of the film morphology and firing temperature
in the range 650–850 °C on CO sensitivity were studied. The best gas response, expressed as the ratio between the resistance
in air and the resistance under gas exposure (R
air/R
gas), was measured for TSN 11 at 11 for 1,000 ppm CO exposure. All types of sensors showed good thermal stability. The electrochemical
impedance spectroscopy (EIS) measurements were performed in different gas atmospheres (air, O2, CO and NO2) to better understand the electrical properties of the nanostructured mixed metal oxides. 相似文献
46.
Elisabetta Fanizza Carmine Urso R. Maria Iacobazzi Nicoletta Depalo Michela Corricelli Annamaria Panniello 《Science and Technology of Advanced Materials》2016,17(1):98-108
Silica based multifunctional heterostructures, exhibiting near infrared (NIR) absorption (650–1200 nm) and luminescence in the visible region, represent innovative nanosystems useful for diagnostic or theranostic applications. Herein, colloidal synthetic procedures are applied to design a photoactive multifunctional nanosystem. Luminescent silica (SiO2) coated quantum dots (QDs) have been used as versatile nanoplatforms to assemble on their surface gold (Au) seeds, further grown into Au spackled structures. The synthesized nanostructures combine the QD emission in the visible region, and, concomitantly, the distinctive NIR absorption of Au nanodomains. The possibility of having multiple QDs in a single heterostructure, the SiO2 shell thickness, and the extent of Au deposition onto SiO2 surface have been carefully controlled. The work shows that a single QD entrapped in 16 nm thick SiO2 shell, coated with Au speckles, represents the most suitable geometry to preserve the QD emission in the visible region and to generate NIR absorption from metal NPs. The resulting architectures present a biomedical potential as an effective optical multimodal probes and as promising therapeutic agents due to the Au NP mediated photothermal effect. 相似文献
47.
Arianna Alfieri Marco Cantamessa Francesca Montagna Elisabetta Raguseo 《Computers & Industrial Engineering》2013,64(2):562-572
Production systems design is a multifaceted task, due to a variety of aspects such as the mutual interdependency between the sub-systems, the variety of configurations and alternative system control strategies, the multiple managerial and “soft” aspects that cast an influence on the behavior of the system. A reasonable number of modeling tools can be applied to production system design, but they tend to divide the problem into unconnected sub-problems whose individual solutions may result in a poor global one. This is despite the fact that production design encompasses all aspects of manufacturing operations, and needs a systemic approach, as clearly shown in practitioner-oriented literature. This paper proposes to apply the “System of Systems” approach to production system design in order to represent their main aspects and support the rational definition of the path leading from corporate strategy to system (re)design. 相似文献
48.
Sabrina Burattini Paola Ferri Michela Battistelli Alessandra D'emilio Laura Biagiotti Piero Sestili Marco B. L. Rocchi Elisabetta Falcieri 《Microscopy research and technique》2009,72(12):913-923
A common pattern of apoptotic death is DNA cleavage, initially producing large fragments (50 kbp), followed by the production of nucleosomic/oligonucleosomic fragments. Nevertheless, apoptosis without DNA fragmentation, at least of the nucleosomic type, has been reported. To investigate the spatial relationship between DNA cleavage and chromatin condensation, we applied the TUNEL technique to the ultrastructural analysis of apoptotic cells. A modified method, utilizing a gold‐conjugated antidigoxigenin antibody, was carried out on U937 versus Molt‐4 cells, both exposed to UVB radiation or staurosporine treatment. Gold particle density in the different domains of apoptotic cells was evaluated by a four‐way ANOVA test. Gold labelling was more strongly localised in condensed chromatin than in the diffuse chromatin. U937 cells, which evidenced in vitro oligonucleosomic fragmentation after both UVB and staurosporine treatments, revealed a significantly higher gold particle density, when compared with Molt‐4, which did not show, on the other hand, oligonucleosomic cleavage even in the presence of ≤50 kbp cleavage. Thus, a correlation between DNA fragment sizes and gold particle density appears. TUNEL applied to electron microscopy is an effective approach to study the relationship between apoptotic chromatin condensation and DNA cleavage. Both these events indeed appear in the apoptotic nucleus, but their reciprocal correlation is still greatly unknown. Microsc. Res. Tech. 2009. © 2009 Wiley‐Liss, Inc. 相似文献
49.
Erica Bazzan Mariaenrica Tin Alvise Casara Davide Biondini Umberto Semenzato Elisabetta Cocconcelli Elisabetta Balestro Marco Damin Claudia Maria Radu Graziella Turato Simonetta Baraldo Paolo Simioni Paolo Spagnolo Marina Saetta Manuel G. Cosio 《International journal of molecular sciences》2021,22(12)
Extracellular vesicles (EVs) are a family of particles/vesicles present in blood and body fluids, composed of phospholipid bilayers that carry a variety of molecules that can mediate cell communication, modulating crucial cell processes such as homeostasis, induction/dampening of inflammation, and promotion of repair. Their existence, initially suspected in 1946 and confirmed in 1967, spurred a sharp increase in the number of scientific publications. Paradoxically, the increasing interest for EV content and function progressively reduced the relevance for a precise nomenclature in classifying EVs, therefore leading to a confusing scientific production. The aim of this review was to analyze the evolution of the progress in the knowledge and definition of EVs over the years, with an overview of the methodologies used for the identification of the vesicles, their cell of origin, and the detection of their cargo. The MISEV 2018 guidelines for the proper recognition nomenclature and ways to study EVs are summarized. The review finishes with a “more questions than answers” chapter, in which some of the problems we still face to fully understand the EV function and potential as a diagnostic and therapeutic tool are analyzed. 相似文献
50.
Giovanna Rassu Elisabetta Gavini Gianpiera Spada Salvatore Marceddu 《Drug development and industrial pharmacy》2013,39(11):1178-1187
The preparation of ketoprofen spray-dried microspheres can be affected by the long drug recrystallization time. Polymer type and drug–polymer ratio as well as manufacturing parameters affect the preparation. The purpose of this work was to evaluate the possibility to obtain ketoprofen spray-dried microspheres using the Eudragit® RS and RL; the influence of the spray-drying parameters on morphology, dimension, and physical stability of microspheres was studied. Ketoprofen microspheres based on Eudragit® blend can be prepared by spray-drying and the nebulization parameters do not influence significantly particle properties; nevertheless, they can be affected by drying and storage methods. No effect of the container material is found. 相似文献