Tragverhalten von Verbundbauteilen aus bewehrtem UHFB und Stahlbeton

Ultra‐Hochleistungs‐Faserbetone (UHFB) eignen sich aufgrund ihrer hohen Festigkeiten, des hohen Verformungsvermögens und der geringen Permeabilität zur Verbesserung und Instandsetzung bestehender Betonbauten. Mit dünnen Schichten von bewehrtem UHFB, die auf bestehende Stahlbetonbauteile aufgetragen werden, können der Tragwiderstand und die Gebrauchstauglichkeit deutlich gesteigert werden. In einer umfangreichen Versuchsreihe wurden die Eigenschaften von mit zusätzlich zu den Fasern auch mit Stabstahl bewehrtem UHFB untersucht. Die Bewehrung des UHFB mit Stabstählen ist vorteilhaft, um den Verfestigungsbereich des UHFB zu erweitern, seinen Tragwiderstand zu erhöhen und die Streuung seiner mechanischen Eigenschaften zu reduzieren. Zur Bewehrung können hoch‐ oder niederfeste Stähle mit unterschiedlichen Oberflächenstrukturen zum Einsatz kommen. Abschließend werden zwei Anwendungen vorgestellt. Structural Behaviour of Composite Elements Combining Reinforced Ultra‐High Performance Fibre‐Reinforced Concrete (UHPFRC) and Reinforced Concrete Due to their high strengths, high deformability and low permeability Ultra‐High Performance Fibre‐Reinforced Concretes (UHPFRC) are suitable for the improvement and rehabilitation of existing concrete structures. Thin layers of reinforced UHPFRC that are applied to existing concrete members, increase both the load bearing capacity and the serviceability. By comprehensive experimental studies the behaviour of UHPFRC with additional bar reinforcement was investigated. The reinforcement of UHPFRC is advantageous in order to increase the strain hardening capacity of UHPFRC, its load bearing capacity and to reduce the scatter of its mechanical properties. Low or high strength steel grades with various surface characteristics can be used as reinforcement of UHPFRC. Finally two on site applications are presented.     

Mass spectrometric QUAL/QUAN approaches for drug metabolism and metabolomics

A liquid chromatography-high-resolution mass spectrometry platform was used for simultaneous qualitative and quantitative (QUAL/QUAN) acquisition, enabling drug metabolism and metabolomics investi- gations. Plasma study samples were monitored for three different groups of patients at a single time-point (1 h after drug administration): one group received acetaminophen (APAP), one group received both APAP and ketorolac and one group was a control group. The quantification of APAP and two of its metabolites (APAP-glucuronide and APAP-cysteine) was performed on a fast acquisition quadrupole-Time-Of-Flight (50-100 ms duty cycle, resolving power of 30,000) compatible with UHPLC time constraints. High-resolution Selected Reaction Monitoring was used for quantification of APAP and its metabolites from 50-10,000 ng/mL using a 50 μL plasma aliquot. Average measured concentrations were for APAP 6,650 ng/mL vs 6,160 ng/mL, APAP-CYS concentrations were 154.2 ng/mL vs 140.6 ng/mL and APAP-GLU concentrations 8,750 ng/mL vs 8,430 ng/mL between the group that received only APAP (n = 11) and the group that received APAP in combination with ketorolac (n = 11). No major differences were observed between the two groups of patients, as it would be expected due to the differing metabolism pathway for both substances. For the qualitative aspect, a metabolomics data processing platform with biological QC samples was applied to the study samples to search for unanticipated metabolites and biomarkers related to APAP and ketorolac metabolism. Multivariate analysis (i.e. Principle Component Analysis), variables grouping tools (i.e. PCVG) and high-resolution MS(/MS) spectra from the MS(ALL) acquisition strategy enabled the profiling and characterization of circulating metabolites of APAP in plasma such as APAP-sulfate, APAP-mercapturate as well as ketorolac.     

Quantification of viable Brochothrix thermosphacta in cooked shrimp and salmon by real-time PCR

Brochothrix thermosphacta, a Gram-positive bacterium, is considered as the predominant spoilage microbiota of modified atmosphere packing (MAP) shrimp and fish. Traditional methods currently used to detect B. thermosphacta in foods are time-consuming and labour-intensive. The aim of this study was to develop a real-time PCR quantification method combined with a propidium monoazide (PMA) sample treatment step to monitor the population of B. thermosphacta in cooked shrimp and salmon. The specificity of the two primers MO405 and MO404 used to amplify a 70 bp fragment of the 16S rRNA gene was demonstrated by using purified DNA from 30 strains, among 21 bacterial species including 22 reference strains. Using these primers for real-time PCR and in pure culture, a good correlation was obtained between real-time PCR and the conventional plating method. Quantification was linear over 7-log units using artificially inoculated samples. The method performed successfully when tested on naturally contaminated cooked shrimp and fresh salmon, with a minimum threshold of 1.9×102 CFU/g for accurate quantification of B. thermosphacta. The correlation between the B. thermosphacta counts obtained by real-time PCR and plate counts on naturally contaminated shrimp and salmon was high (R2=0.895). Thus, this study presents a rapid tool for producing reliable quantitative data on B. thermosphacta in cooked shrimp and fresh salmon.     

Sensing with multipolar second harmonic generation from spherical metallic nanoparticles

We show that sensing in the nonlinear optical regime using multipolar surface plasmon resonances is more sensitive in comparison to sensing in the linear optical regime. Mie theory, and its extension to the second harmonic generation from a metallic nanosphere, is used to describe multipolar second harmonic generation from silver metallic nanoparticles. The standard figure of merit of a potential plasmonic sensor based on this principle is then calculated. We finally demonstrate that such a sensor is more sensitive to optical refraction index changes occurring in the vicinity of the metallic nanoparticle than its linear counterpart.     

Extremely high tunability and low loss in nanoscaffold ferroelectric films

There are numerous radio frequency and microwave device applications which require materials with high electrical tunability and low dielectric loss. For phased array antenna applications there is also a need for materials which can operate above room temperature and which have a low temperature coefficient of capacitance. We have created a nanoscaffold composite ferroelectric material containing Ba(0.6)Sr(0.4)TiO(3) and Sm(2)O(3) which has a very high tunability which scales inversely with loss. This behavior is opposite to what has been demonstrated in any previous report. Furthermore, the materials operate from room temperature to above 150 °C, while maintaining high tunability and low temperature coefficient of tunability. This new paradigm in dielectric property control comes about because of a vertical strain control mechanism which leads to high tetragonality (c/a ratio of 1.0126) in the BSTO. Tunability values of 75% (200 kV/cm field) were achieved at room temperature in micrometer thick films, the value remaining to >50% at 160 °C. Low dielectric loss values of <0.01 were also achieved, significantly lower than reference pure films.     

A comparison between thermosonics and thermography for delamination detection in polymer matrix laminates

Over recent decades, industry has used more and more frequently composite laminates. If many Non-Destructive Testing (NDT) methods have already been developed to test such structures, detecting defects in polymer matrix laminates remains difficult. After a short overview of the main existing NDT methods, this paper focuses on two techniques which appear to be very promising: thermosonics and infra-red (IR) thermography. For thermosonics tests, an ultrasonic horn mechanically loads the sample, while an IR camera records the defect heating. For thermography tests, the heating is due to an optical wave. If minor improvements on the test device are presented for thermosonics tests, IR thermography tests are achieved with a CO₂ laser as excitation source, which is a very innovative approach. This paper provides many experimental results obtained from an aeronautical carbon-epoxy plate with a central delamination. It is shown that both methods are very suitable for detecting such a defect, even if each method has its own advantages. Finally, some improvements to further optimize the thermography tests are proposed in conclusion.     

Optical properties of HgTe colloidal quantum dots

Room temperature photodetection with HgTe colloidal quantum films is reported between 2 and 5 μm for particles of sizes between ~5 and ~12 nm diameter, and photodetection extends to 7 μm at 80 K. The size-tuning of the absorption of HgTe colloidal quantum dots, their optical cross section and the infrared absorption depth of films are measured. The tuning with radius is empirically given by [see formula in text] where R is in nm. The optical cross section of the colloidal dots at 415 nm is approximately proportional to their volume and given by σ(Hg)(415) = 2.6 ± 0.4 10(-17) cm(2)/mercury atom. The size-dependent optical cross section at the band edge ~1.5 10(-15) cm(2) is consistent with the expected oscillator strength of the quantum dots. The absorption depth of HgTe colloidal dot films is short, about 1-2 μm, which is an advantage for thin film devices. These properties agree rather well with the expectation from the k · p model. HgTe colloidal quantum dot thin films show a strong tuning with temperature with a large positive thermal shift between 0.4 and 0.2 meV K(-1), decreasing with decreasing size within the size range studied and this is attributed primarily to electron-phonon effects.     

Impact of Gram-negative bacteria in interaction with a complex microbial consortium on biogenic amine content and sensory characteristics of an uncooked pressed cheese

The impact of Gram-negative bacteria on sensory characteristics and production of volatile compounds as well as biogenic amines (BA) in the core of an uncooked pressed type model cheese was investigated in the presence of a defined complex microbial consortium. Eleven strains of Gram-negative bacteria, selected on the basis of their biodiversity and in vitro BA-production ability, were individually tested in a model cheese. Four out of 6 strains of Enterobacteriaceae (Citrobacter freundii UCMA 4217, Klebsiella oxytoca 927, Hafnia alvei B16 and Proteus vulgaris UCMA 3780) reached counts close to 6 log CFU g⁻¹ in the model cheese. In core of cheeses inoculated with Gram-negative bacteria, only slight differences were observed for microbial counts (Enterococcus faecalis or Lactobacillus plantarum count differences below 1 log CFU g⁻¹), acetate concentration (differences below 200 mg kg⁻¹) and texture (greater firmness) in comparison to control cheeses. Cheese core colour, odour and volatile compound composition were not modified. Although ornithine, the precursor of putrescine, was present in all cheeses, putrescine was only detected in cheeses inoculated with H. alvei B16 and never exceeded 2.18 mmol kg⁻¹ cheese dry matter. Cadaverine was only detected in cheeses inoculated with H. alvei B16, K. oxytoca 927, Halomonas venusta 4C1A or Morganella morganii 3A2A but at lower concentrations (<1.05 mmol kg⁻¹ cheese dry matter), although lysine was available. Only insignificant amounts of the detrimental BA histamine and tyramine, as well as isopentylamine, tryptamine or phenylethylamine, were produced in the cheese model by any of the Gram-negative strains, including those which produced these BA at high levels in vitro.     

Acyclic and k-distance coloring of the grid

In this paper, we give a relatively simple though very efficient way to color the d-dimensional grid G(n₁,n₂,…,n_d) (with n_i vertices in each dimension 1?i?d), for two different types of vertex colorings: (1) acyclic coloring of graphs, in which we color the vertices such that (i) no two neighbors are assigned the same color and (ii) for any two colors i and j, the subgraph induced by the vertices colored i or j is acyclic; and (2) k-distance coloring of graphs, in which every vertex must be colored in such a way that two vertices lying at distance less than or equal to k must be assigned different colors. The minimum number of colors needed to acyclically color (respectively k-distance color) a graph G is called acyclic chromatic number of G (respectively k-distance chromatic number), and denoted a(G) (respectively χ_k(G)).The method we propose for coloring the d-dimensional grid in those two variants relies on the representation of the vertices of G_d(n₁,…,n_d) thanks to its coordinates in each dimension; this gives us upper bounds on a(G_d(n₁,…,n_d)) and χ_k(G_d(n₁,…,n_d)).We also give lower bounds on a(G_d(n₁,…,n_d)) and χ_k(G_d(n₁,…,n_d)). In particular, we give a lower bound on a(G) for any graph G; surprisingly, as far as we know this result was never mentioned before. Applied to the d-dimensional grid G_d(n₁,…,n_d), the lower and upper bounds for a(G_d(n₁,…,n_d)) match (and thus give an optimal result) when the lengths in each dimension are “sufficiently large” (more precisely, if ). If this is not the case, then these bounds differ by an additive constant at most equal to . Concerning χ_k(G_d(n₁,…,n_d)), we give exact results on its value for (1) k=2 and any d?1, and (2) d=2 and any k?1.In the case of acyclic coloring, we also apply our results to hypercubes of dimension d, H_d, which are a particular case of G_d(n₁,…,n_d) in which there are only 2 vertices in each dimension. In that case, the bounds we obtain differ by a multiplicative constant equal to 2.