Antonella Brizzi  Samuele Maramai  Francesca Aiello  Maria Camilla Baratto  Federico Corelli  Claudia Mugnaini  Marco Paolino  Francesco Scorzelli  Carlo Aldinucci  Luciano De Petrocellis  Cinzia Signorini  Federica Pessina 《International journal of molecular sciences》2022,23(21)

α-Lipoic acid is a sulfur-containing nutrient endowed with pleiotropic actions and a safe biological profile selected to replace the unsaturated alkyl acid of capsaicin with the aim of obtaining lipoic amides potentially active as a TRPV1 ligand and with significant antioxidant properties. Thus, nine compounds were obtained in good yields following a simple synthetic procedure and tested for their functional TRPV1 activity and radical-scavenger activity. The safe biological profile together with the protective effect against hypoxia damage as well as the in vitro antioxidant properties were also evaluated. Although less potent than capsaicin, almost all lipoic amides were found to be TRPV1 agonists and, specifically, compound 4, the lipoic analogue of capsaicin, proved to be the best ligand in terms of efficacy and potency. EPR experiments and in vitro biological assays suggested the potential protective role against oxidative stress of the tested compounds and their safe biological profile. Compounds 4, 5 and 9 significantly ameliorated the mitochondrial membrane potential caused by hypoxia condition and decreased F2-isoprostanes, known markers of oxidative stress. Thus, the experimental results encourage further investigation of the therapeutic potential of these lipoic amides.  相似文献   

    

Laura Caliogna  Valentina Bina  Alice Maria Brancato  Giulia Gastaldi  Salvatore Annunziata  Mario Mosconi  Federico Alberto Grassi  Francesco Benazzo  Gianluigi Pasta 《International journal of molecular sciences》2022,23(22)

Bone responses to pulsed electromagnetic fields (PEMFs) have been extensively studied by using devices that expose bone cells to PEMFs to stimulate extracellular matrix (ECM) synthesis for bone and cartilage repair. The aim of this work was to highlight in which bone healing phase PEMFs exert their action. Specifically, we evaluated the effects of PEMFs both on human adipose mesenchymal stem cells (hASCs) and on primary human osteoblasts (hOBs) by testing gene and protein expression of early bone markers (on hASCs) and the synthesis of late bone-specific proteins (on hOBs) as markers of bone remodeling. Our results indicate that PEMFs seem to exert their action on bone formation, acting on osteogenic precursors (hASCs) and inducing the commitment towards the differentiation pathways, unlike mature and terminally differentiated cells (hOBs), which are known to resist homeostasis perturbation more and seem to be much less responsive than mesenchymal stem cells. Understanding the role of PEMFs on bone regenerative processes provides important details for their clinical application.  相似文献   

    

Mariangela Pucci  Claudio D&#x;Addario  Emanuela Micioni Di Bonaventura  Francesca Mercante  Eugenia Annunzi  Federico Fanti  Manuel Sergi  Luca Botticelli  Giacomo Einaudi  Carlo Cifani  Maria Vittoria Micioni Di Bonaventura 《International journal of molecular sciences》2022,23(23)

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Martina Bollati  Chiara Lopez  Fabio Bioletto  Federico Ponzetto  Ezio Ghigo  Mauro Maccario  Mirko Parasiliti-Caprino 《International journal of molecular sciences》2022,23(4)

Primary aldosteronism (PA) is the most common cause of secondary hypertension. A growing body of evidence has suggested that, beyond its well-known effects on blood pressure and electrolyte balance, aldosterone excess can exert pro-inflammatory, pro-oxidant and pro-fibrotic effects on the kidney, blood vessels and heart, leading to potentially harmful pathophysiological consequences. In clinical studies, PA has been associated with an increased risk of cardiovascular, cerebrovascular, renal and metabolic complication compared to essential hypertension, including atrial fibrillation (AF) and aortic ectasia. An increased prevalence of AF in patients with PA has been demonstrated in several clinical studies. Aldosterone excess seems to be involved in the pathogenesis of AF by inducing cardiac structural and electrical remodeling that in turn predisposes to arrhythmogenicity. The association between PA and aortic ectasia is less established, but several studies have demonstrated an effect of aldosterone on aortic stiffness, vascular smooth muscle cells and media composition that, in turn, might lead to an increased risk of aortic dilation and dissection. In this review, we focus on the current evidence regarding the potential role of aldosterone excess in the pathogenesis of AF and aortic ectasia.  相似文献   

    

Valeria Libera  Federico Bianchi  Barbara Rossi  Francesco D&#x;Amico  Claudio Masciovecchio  Caterina Petrillo  Francesco Sacchetti  Alessandro Paciaroni  Lucia Comez 《International journal of molecular sciences》2022,23(9)

G-quadruplexes (G4s) are noncanonical forms of DNA involved in many key genome functions. Here, we exploited UV Resonance Raman scattering to simultaneously explore the vibrational behavior of a human telomeric G4 (Tel22) and its aqueous solvent as the biomolecule underwent thermal melting. We found that the OH stretching band, related to the local hydrogen-bonded network of a water molecule, was in strict relation with the vibrational features of the G4 structure as a function of temperature. In particular, the modifications to the tetrahedral ordering of the water network were strongly coupled to the DNA rearrangements, showing changes in temperature that mirrored the multi-step melting process of Tel22. The comparison between circular dichroism and Raman results supported this view. The present findings provide novel insights into the impact of the molecular environment on G4 conformation. Improving current knowledge on the solvent structural properties will also contribute to a better understanding of the role played by water arrangement in the complexation of G4s with ligands.  相似文献   

    

Lorenzo Barba  Steffen Halbgebauer  Federico Paolini Paoletti  Giovanni Bellomo  Samir Abu-Rumeileh  Petra Steinacker  Federico Massa  Lucilla Parnetti  Markus Otto 《International journal of molecular sciences》2022,23(13)

SerpinA1 (α1-antitrypsin) is a soluble glycoprotein, the cerebrospinal fluid (CSF) isoforms of which showed disease-specific changes in neurodegenerative disorders that are still unexplored in Alz-heimer’s disease (AD). By means of capillary isoelectric focusing immunoassay, we investigated six serpinA1 isoforms in CSF samples of controls (n = 29), AD-MCI (n = 29), AD-dem (n = 26) and Lewy body disease (LBD, n = 59) patients and correlated the findings with CSF AD core biomarkers (Aβ42/40 ratio, p-tau, t-tau). Four CSF serpinA1 isoforms were differently expressed in AD patients compared to controls and LBD patients, especially isoforms 2 and 4. AD-specific changes were found since the MCI stage and significantly correlated with decreased Aβ42/40 (p < 0.05) and in-creased p-tau and t-tau levels in CSF (p < 0.001). Analysis of serpinA1 isoform provided good di-agnostic accuracy in discriminating AD patients versus controls (AUC = 0.80) and versus LBD patients (AUC = 0.92), with best results in patients in the dementia stage (AUC = 0.97). SerpinA1 isoform expression is altered in AD patients, suggesting a common, albeit disease-specific, in-volvement of serpinA1 in most neurodegenerative disorders.  相似文献   

    

Valentino Le Noci  Giancarla Bernardo  Giacomo Manenti  Gabriele Infante  Dariush Khaleghi Hashemian  Lucia Minoli  Simone Canesi  Francesca Bianchi  Tiziana Triulzi  Stefania Arioli  Loris De Cecco  Simone Guglielmetti  Federico Ambrogi  Camilla Recordati  Nicoletta Gagliano  Elda Tagliabue  Michele Sommariva  Lucia Sfondrini 《International journal of molecular sciences》2022,23(21)

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Marisa Iborra  Ins Moret  Enrique Bus  Jos Luis García-Gimnez  Elena Ricart  Javier P. Gisbert  Eduard Cabr  Maria Esteve  Lucía Mrquez-Mosquera  Esther García-Planella  Jordi Guardiola  Federico V. Pallard  Carolina Serena  Francisco Algaba-Chueca  Eugeni Domenech  Pilar Nos  Beln Beltrn 《International journal of molecular sciences》2022,23(24)

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Federico Smeacetto  Andreas Chrysanthou  Antonio G. Sabato  Hassan Javed  Stefano De la Pierre  Milena Salvo  Monica Ferraris 《International Journal of Applied Ceramic Technology》2022,19(2):1017-1028

A summary and update of the research on the design, process, and testing of glass and glass-ceramic to metal seals for solid oxide cells (SOC) conducted during the last 13 years at the Politecnico di Torino, Italy, within our research group and with several international collaborations, is presented and discussed. Glass and glass-ceramic-based materials play a key role in SOC performance and durability as well as in the sealing and joining of metallic interconnects. The sinter-crystallization method is a powerful technique that is used to obtain dense and reliable glass-ceramics capable of providing hermetic joining and seals. The issues, learned from our experience, that have an impact on the lifetime behavior of glass and glass-ceramic to metal seals are briefly reviewed in terms of challenges and working conditions. The mechanical characterization of glass-to-metal seal materials has a crucial impact on the overall performance, as the metal–glass interface plays a key role on the final joining strength and reliability. Several glass compositions are presented along with results on their characterization and behavior as joining materials for metallic interconnects, with a focus on their potential use as SOC seals.  相似文献   

    

Perepichka DF  Rosei F 《Small (Weinheim an der Bergstrasse, Germany)》2006,2(1):22-25

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