Visualization of mitochondria with green fluorescent protein in cultured fibroblasts from patients with mitochondrial diseases

cDNAs for green fluorescent protein (GFP) and for a GFP fusion protein containing the presequence of human ornithine transcarbamylase (pOTC-GFP) were transfected into cultured human fibroblasts. GFP cDNA gave diffuse fluorescence throughout the cytoplasm and the nucleus, whereas pOTC-GFP cDNA gave mitochondria-associated fluorescence. Fluorescent mitochondrial structures could be classified into five patterns: thread-like mitochondria, fine thread-like ones, rod-like ones, granular ones, and granular ones with weak cytosolic fluorescence. pOTC-GFP mutants resulted in a loss of mitochondrial fluorescence and an appearance of weak fluorescence throughout the cytoplasm. pOTC-GFP cDNA was transfected into fibroblasts from patients with various mitochondrial diseases. Higher ratios of fibroblasts with granular mitochondria and those with fine thread-like ones were observed in a patient with Reye's syndrome and a patient with Kearns-Sayre syndrome. Weak cytosolic fluorescence was sometimes observed in fibroblasts from these patients. This method will be useful to analyze mitochondrial structural alterations and disorders of mitochondrial protein import.     

Thermal stability of reinforced Nb3Sn composite superconductor under cryocooled conditions

Several types of reinforced Nb₃Sn wires have been developed to prevent reduction of superconducting properties by applying a strong electromagnetic force. To fabricate a cryocooled magnet using those reinforced wires, we experimentally measured the minimum quench energy (MQE) under cryocooled conditions of some reinforced Nb₃Sn wires. As a result, it became clear that thermal stability expressed as MQE was controlled by the temperature margin between the temperature of the operating condition and the transition temperature from superconductivity to normal. Using the FEM analysis, it was realized that the cause of the decline in thermal stability for the reinforced wires was the low thermal conductivity of the reinforced materials.     

Bioeffects of Low Energy Ion-Beam Implantation and Vacuum Treatment on M1 Arabidopsis Thaliana

Vacuum treatment and ion-beam bombardment are two major processes in the low energy ion-beam implantation. To accurately study the contributions of these two major factors to the bioeffects separately, the M1 generation variation of Arabidopsis thaliana with ion-beam implantation and vacuum treatment were compared through a series of key plant development parameters including morphological observation, biochemical assay and RAPD （random amplified polymorphic DNA） analysis. The results showed that ion-beam implantation had obvious effect on almost all of these parameters, and the vacuum treatment had some impacts on several morphological parameters such as the bolting time and the length of the primary stem. Taking the results together, the indication is that vacuum treatment has some slight contributions to the bioeffects of ion-beam implantation while ion-beam bombardment itself is the major creator of the bioeffects.     

紧贴保护体钢筋砼老闸墩控爆拆除

介绍了特大体积闸墩爆破拆除时爆破参数的选取和实施方法。新老闸墩一端仅隔4cm，利用钻孔爆破法拆除老闸墩取得了快速，经济，安全的爆破效果。     

烟机异型件CAD/CAM系统的开发与应用

结合企业异型件CAD/CAM系统的开发与应用实际，简要说明了应用CAD/CAM技术是实现烟草机械异型件国产化的必要途径，论述了CAD/CAM系统的开发所依据的技术原理和涉及到的关键技术问题，分析了应用该系统所产生的社会经济效益．     

堆存温度对半水磷石膏胶凝性能影响

半水磷石膏（HPG）长时间堆存状态下会出现固结现象,其胶凝性能也相应下降。以室内HPG结晶水检测和单轴压缩试验为基础,通过设定4种不同堆存温度,分别为20,40,60和80 ℃,探究不同堆存温度作用下HPG试样结晶水质量分数变化和堆存后制备的充填胶凝材料（HCM）抗压强度发展规律,并采用扫描电镜等微观分析手段研究堆存温度对其强度影响机制。结果表明,堆存温度对HPG胶凝性能影响显著,高的堆存温度会加快HPG试样中的自由水转变为结晶水速率,而且会抑制堆存后制备的HCM强度发展。采用数据标准化对不同堆存温度作用后的试样抗压强度作出预测,被证实与实测值较吻合。微观分析发现,堆存温度主要影响体系的过饱和度,而使不同堆存温度作用后制备的HCM微观形态表现差异。      

An adaptive genetic algorithm for the time dependent inventory routing problem

In this paper we propose an adaptive genetic algorithm that produces good quality solutions to the time dependent inventory routing problem (TDIRP) in which inventory control and time dependent vehicle routing decisions for a set of retailers are made simultaneously over a specific planning horizon. This work is motivated by the effect of dynamic traffic conditions in an urban context and the resulting inventory and transportation costs. We provide a mixed integer programming formulation for TDIRP. Since finding the optimal solutions for TDIRP is a NP-hard problem, an adaptive genetic algorithm is applied. We develop new genetic representation and design suitable crossover and mutation operators for the improvement phase. We use adaptive genetic operator proposed by Yun and Gen (Fuzzy Optim Decis Mak 2(2):161–175, 2003) for the automatic setting of the genetic parameter values. The comparison of results shows the significance of the designed AGA and demonstrates the capability of reaching solutions within 0.5 % of the optimum on sets of test problems.     

取代基及分子量对非离子型纤维素醚表面特性的影响

根据Washburn的浸渍理论(Penetration Theory)和van Oss-Good- Chaudhury的组合理论(Combining Theory)及应用柱状灯芯技术(Column Wicking Technique),对几种非离子型纤维素醚,如甲基纤维素、羟丙基纤维素和羟丙基甲基纤维素的表面特性进行了测试。由于这些纤维素醚的取代基、取代度和分子量不同,所以它们的表面能及其组成部分有着明显的差异。数据说明,非离子型纤维素醚Lewis碱大于Lewis酸,表面自由能的主要成分是Lifshitz-van der Waals力。羟丙基的表面能及其成分都大于羟甲基。而在相同取代基和取代度的前提下,羟丙基纤维素的表面自由能正比于分子量;而羟丙基甲基纤维素的表面自由能则正比于取代度,反比于分子量。实验还发现非离子型纤维素醚中的取代基羟丙基和羟丙基甲基的表面能似乎都大于纤维素的表面能,而实验证明所测试得出的纤维素的表面能及其成分的数据是与文献所吻合的。     

絮凝沉淀,过滤处理合成苯乙烯污水

本文通过对合成苯乙烯污水水质考察，提出了利用污水中本身所含有的ＡｌＣｌ３作为絮凝剂，对该污水进行絮凝沉淀过滤工艺处理。试验结果表明，处理后污水ＣＯＤ、ＰＨ值１、悬浮物含量等各项指标均能达到新建污水处理装置的排放标准，此工艺个有投资少、运行简单、易操作管理、处理成本低等特点。     

雷达装备维修保障力量评估建模研究

为评估雷达装备维修保障力量,通过分析维修保障力量组织结构和运行方式,构建了雷达装备维修保障力量评价指标体系,并采用AHP方法给出了评估步骤．通过实例验证该模型是有效的,其评估结果可为优化雷达装备维修保障力量,提高维修保障效能提供有力的帮助．