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51.
Serena Bianchi Sara Bernardi Antonella Mattei Loredana Cristiano Leonardo Mancini Diana Torge Giuseppe Varvara Guido Macchiarelli Enrico Marchetti 《International journal of molecular sciences》2022,23(9)
Several types of deproteinised bovine bone mineral (DBBM) are available on the market, and each one is obtained with a thermic and chemical process that can differ, achieving different results. Currently, several protocols using low temperature are suggested to reduce the possible particle crystallisation during the production process. This study aimed to evaluate the biomorphological reaction of periodontal fibroblast cultures in contact with different DBBM particles treated with a low-temperature protocol (Thermagen®) and without exposure to sodium hydroxide (NaOH). Morphological evaluation was performed using light, confocal laser, and scanning electron microscopy, and the biological reaction in terms of proliferation was performed using an XTT proliferation assay at 24 h (T1), 72 h (T2), and 7 days (T3). The morphological analysis highlighted how the presence of the materials stimulated a change in the morphology of the cells into a polygonal shape, surface reactions with the thickening of the membrane, and expression of actin. In particular, the morphological changes were appreciable from T1, with a progressive increase in the considered morphological characteristics at T2 and T3 follow-ups. The proliferation assay showed a statistical significance between the different experimental materials and the negative control in T2 and T3 follow-ups. The post hoc analysis did not reveal any differences between the materials. In conclusion, the grafts obtained with the low-temperature extractions protocol and not exposed to NaOH solution showed positive morphological reactions with no differences in the sizes of particles. 相似文献
52.
Alessandro Di Minno Monica Gelzo Marianna Caterino Michele Costanzo Margherita Ruoppolo Giuseppe Castaldo 《International journal of molecular sciences》2022,23(9)
Metabolomics helps identify metabolites to characterize/refine perturbations of biological pathways in living organisms. Pre-analytical, analytical, and post-analytical limitations that have hampered a wide implementation of metabolomics have been addressed. Several potential biomarkers originating from current targeted metabolomics-based approaches have been discovered. Precision medicine argues for algorithms to classify individuals based on susceptibility to disease, and/or by response to specific treatments. It also argues for a prevention-based health system. Because of its ability to explore gene–environment interactions, metabolomics is expected to be critical to personalize diagnosis and treatment. Stringent guidelines have been applied from the very beginning to design studies to acquire the information currently employed in precision medicine and precision prevention approaches. Large, prospective, expensive and time-consuming studies are now mandatory to validate old, and discover new, metabolomics-based biomarkers with high chances of translation into precision medicine. Metabolites from studies on saliva, sweat, breath, semen, feces, amniotic, cerebrospinal, and broncho-alveolar fluid are predicted to be needed to refine information from plasma and serum metabolome. In addition, a multi-omics data analysis system is predicted to be needed for omics-based precision medicine approaches. Omics-based approaches for the progress of precision medicine and prevention are expected to raise ethical issues. 相似文献
53.
Nicola Barbieri Giuseppe Manco Ettore Ritacco Marco Carnuccio Antonio Bevacqua 《Machine Learning》2013,93(1):5-29
Probabilistic topic models are widely used in different contexts to uncover the hidden structure in large text corpora. One of the main (and perhaps strong) assumption of these models is that generative process follows a bag-of-words assumption, i.e. each token is independent from the previous one. We extend the popular Latent Dirichlet Allocation model by exploiting three different conditional Markovian assumptions: (i) the token generation depends on the current topic and on the previous token; (ii) the topic associated with each observation depends on topic associated with the previous one; (iii) the token generation depends on the current and previous topic. For each of these modeling assumptions we present a Gibbs Sampling procedure for parameter estimation. Experimental evaluation over real-word data shows the performance advantages, in terms of recall and precision, of the sequence-modeling approaches. 相似文献
54.
Chiara Manneschi Paola Fanzio Elena Angeli Giuseppe Firpo Luca Ceseracciu Valentina Mussi Luca Repetto Ugo Valbusa 《Microfluidics and nanofluidics》2013,14(1-2):21-30
Peculiar transport phenomena appear at nanoscale, since surface effects strongly affect the behaviour of fluids. Electrostatic and steric interactions, capillary forces and entropic effects play a key role in the behaviour of fluids and biomolecules. Since these effects strongly depend on the size of the nanofluidic system, a careful characterization of the fluidic environment is necessary. Moreover, the possibility to dynamically modulate the size of nanochannels is very appealing in the field of biomolecule manipulation. Recently, we have developed a lab-on-chip made of poly(dimethylsiloxane) (PDMS). This polymeric device is based on a tuneable nanochannel able to dynamically change its dimension in order to fit the application of interest. In fact, a mechanical compression applied on the top of the elastomeric device squeezes the nanochannel, reducing the channel cross section and allowing a dynamical optimization of the nanostructures. In this paper, this squeezing process is fully characterized both numerically and experimentally. This analysis provides information on the reduction of the nanochannel dimensions induced by compression as a function of the work of adhesion and of the stiffness of the materials composing the device. Moreover, calculations demonstrate the possibility to predict the change of the nanochannel size and shape induced by the compression. The possibility to dynamically tune the channel size opens up new opportunities in biomolecular sensing or sieving and in the study of new hydrodynamics effects. 相似文献
55.
Antonio Iera Antonella Molinaro Stefano Yuri Paratore Giuseppe Ruggeri Antonella Zurzolo 《Ad hoc Networks》2011,9(8):1414-1429
This paper aims at answering the question whether it is practical to build a mesh-phone or not. A mesh-phone is a smartphone acting as a mesh router, i.e. either actively participating in the process of packet forwarding towards a mesh gateway, or acting itself as a gateway toward the Internet. Technical aspects of the mesh-phone implementation, such as design constraints and implementation details, are addressed. The technical feasibility is validated by describing the implementation of a prototype of the mesh-enabled phone over the OpenmokoTM open platform. Besides technical feasibility also convenience of the addressed solution is analyzed in terms of the consumed power, and some “energy-saving” related enhancements are suggested to be applied to future user’s devices. 相似文献
56.
Alternating current potential drop (ACPD) is an established technique for non-destructive testing and materials characterisation. Finite element (FE) simulation is a very useful tool for the optimisation of probes and the prediction of test results, but three-dimensional models of ACPD problems are computationally intensive. This paper presents a new, simple model in which frequency-related effects are taken into account by appropriately modifying the geometry of the modelled structure: a DC analysis can then be performed on a smaller domain, thus reducing the computational power required. The results of experimental tests for the validation of the model are also presented and discussed. 相似文献
57.
Sergio Brovelli Francesco Meinardi Gustaf Winroth Oliver Fenwick Giuseppe Sforazzini Michael J. Frampton Leszek Zalewski James A. Levitt Francesco Marinello Piero Schiavuta Klaus Suhling Harry L. Anderson Franco Cacialli 《Advanced functional materials》2010,20(2):272-280
Here, it is demonstrated that energy transfer in a blend of semiconducting polymers can be strongly reduced by non‐covalent encapsulation of one constituent, ensured by threading of the conjugated strands into functionalized cyclodextrins. Such macrocycles control the minimum intermolecular distance of chromophores with similar alignment, at the nanoscale, and therefore the relevant energy transfer rates, thus enabling fabrication of white‐light‐emitting diodes (CIE coordinates: x = 0.282, y = 0.336). In particular, white electroluminescence in a binary blend of a blue‐emitting, organic‐soluble rotaxane based on a polyfluorene derivative and the green‐emitting poly(9,9‐dioctylfluorene‐alt‐benzothiadiazole ( F8BT ) is achieved. Morphological and structural analyses by atomic force microscopy, fluorescence mapping, µ‐Raman, and fluorescence lifetime microscopy are used to complement optical and electroluminescence characterization, and to enable a deeper insight into the properties of the novel blend. 相似文献
58.
Liliana Caristia Giuseppe Nicotra Corrado Bongiorno Nicola Costa Sebastiano Ravesi Salvo Coffa Riccardo De Bastiani Maria Grazia Grimaldi Corrado Spinella 《Microelectronics Reliability》2007,47(4-5):777
This work reports the study concerning the influence of the preparation conditions on the structure of silicon rich oxide (SRO) deposited by PECVD method by which the structural properties of the film are strictly related. In particular we investigated the role of reactant gases N2O and SiH4 on the total Si concentration, Si excess concentration, Si clustered concentration and size of nanoclusters formed by high annealing temperature. We payed particular attention on the role of the hydrogen and nitrogen during the Si agglomeration.The presence of hydrogen atoms on the as-deposited specimen, confirmed by the Si–H bonds peak on the FTIR analysis, has been directly correlated to the silicon excess concentration in the layer. The silicon, oxygen and nitrogen atomic density has been calculated from RBS analysis. These information were coupled to the ones obtained using methodology based on electron energy loss spectroscopy combined with energy filtered images, which allowed us to quantify the clustered silicon concentration in annealed sub-stoichiometric silicon oxide layers (SiOx). We have verified that the nitrogen dissolved in the layer inhibits the Si excess clustering so that the efficiency of silicon agglomeration process decreases as the nitrogen content increases. 相似文献
59.
High-computing speed and modularity have made RNS-based arithmetic processors attractive for a long time, especially in signal processing, where additions and multiplications are very frequent. The VLSI technology renewed this interest because RNS-based circuits are becoming more feasible; however, intermodular operations degradate their performance and a great effort results on this topic. In this paper, we deal with the problem of performing the basic operationX(modm), that is the remainder of the integer divisionX/m, for large values of the integerX, following an approximating and correcting approach, which guarantees the correctness of the result. We also define a structure to computeX(modm) by means of few fast VLSI binary multipliers, which is exemplified for 32-bit long numbers, obtaining a total response time lower than 200 nsec. Furthermore, such a structure is evaluated in terms of VLSI complexity and area and time figuresA=?(n 2 T m 2 ) andT=?(T M ) for the parameterT M in \([\log n,\sqrt n ]\) are derived. A simple positional-to-residue converter is finally presented, based on this structure; it improves some complexity results previously obtained by authors. 相似文献
60.
Natale Aiello Giuseppe Di Stefano 《今日电子》2001,(10):34-36
1.技术概述 VK05CFL是一种用于驱动微型荧光灯的自振荡转换器,采用意法半导体的VIPower M3-3专利技术制造而成,允许在同一芯片上集成控制部分和电源级。电源级是一个“发射器开关”,其构成是在级联配置内放置一个双极高压复合晶体管和一个低压MOSFET,因此,在高击穿电 相似文献