Generation of Nonlinear Force Driven Blocks from Skin Layer Interaction of Petawatt-Picosecond Laser Pulses for ICF

The discovery of the essential difference of maximum ion energy for TW-ps laser plasma interaction compared with the 100 ns laser pulses led to the theory of a skin layer model where the control of prepulses suppressed the usual relativistic self-focusing.The subsequent generation of two nonlinear force driven blocks has been demonstrated experimentally and in extensive numerical studies where one block moves against the laser light and the other block into the irradiated target.These blocks of nearly solid state density DT plasma correspond to ion beam current densities exceeding 10^10 A/cm^2 where the ion velocity can be chosen up to highly relativistic values.Using the results of the expected ignition of DT fuel by light ion beams,a selfsustained fusion reaction front may be generated even into uncompressed solid DT fuel similar to the Nuckolls-Wood scheme where 10 kJ laser pulses produce 100 MJ fusion energy.This new and simplified scheme of laser-ICF needs and optimisation of the involved parameters.     

Assessment of In vivo behavior of polymer tube nerve grafts simultaneously with the peripheral nerve regeneration process using scanning electron microscopy technique

In this study, scanning electron microscopy (SEM) has been applied for instantaneous assessment of processes occurring at the site of regenerating nerve. The technique proved to be especially useful when an artificial implant should have been observed but have not yet been extensively investigated before for assessment of nerve tissue. For in vivo studies, evaluation of implant's morphology and its neuroregenerative properties is of great importance when new prototype is developed. However, the usually applied histological techniques require separate and differently prepared samples, and therefore, the results are never a 100% comparable. In our research, we found SEM as a technique providing detailed data both on an implant behavior and the nerve regeneration process inside the implant. Observations were carried out during 12‐week period on rat sciatic nerve injury model reconstructed with nerve autografts and different tube nerve grafts. Samples were analyzed with haematoxylin‐eosin (HE), immunocytochemical staining for neurofillament and S‐100 protein, SEM, TEM, and the results were compared. SEM studies enabled to obtain characteristic pictures of the regeneration process similarly to TEM and histological studies. Schwann cell transformation and communication as well as axonal outgrowth were identified, newly created and matured axons could be recognized. Concurrent analysis of biomaterial changes in the implant (degradation, collapsing of the tube wall, migration of alginate gel) was possible. This study provides the groundwork for further use of the described technique in the nerve regeneration studies. SCANNING 35: 232‐245, 2013. © 2012 Wiley Periodicals, Inc.     

An experimental study on hydrodynamics of a system in a pneumatic flash dryer

Pressure drop dependences on gas and solid flow rates and solid concentrations for a vertical pipe section and for a conical—cylindrical—conical chamber have been determined. Equations for a particle—wall friction factor have been obtained. Values of particle—wall friction factor for a vertical pipe section are in reasonable agreement with literature data. For a conical—cylindrical—conial chamber friction factors can be both positive and negative.     

Simultaneous removal of SO2, SO3 and H2SO4 mist from the gas after TiO2 calcining

Absorption of SO₂ and SO₃ in the solutions of waste ferrous sulfate (so-called ‘green salt’) and in the spent acid after TiO₂ hydrolysis, at H₂SO₄ concentrations ranging from 0–5 to 15 g/m³ (STP), was studied. The rate of SO₃ absorption has been found to rise linearly with increasing SO₃ concentration in the gas and to be independent on H₂SO₄ concentration in solution. The SO₂ absorption also rises linearly with increasing SO₂ content in the gas, but diminishes as H₂SO₄ concentration increases—an upper limit of 100 g H₂SO₄/kg H₂O is indicated. The initial concentration of the solution must not be higher than 40 g H₂SO₄/kg H₂O.     

Kaltgewalztes Flachzeug aus unlegierten weichen Stählen — Nach DIN 1623 Blatt 1

Cold-Rolled Flat Products of Plain Carbon Mild Steel According to DIN 1623 Sheet 1 . Cold-rolled flat products of plain carbon mild steel and among the most important finished products made by iron and steel works and rolling mills as well as being among the most important semi-products for industrial manufacture. Metallurgical and rolling developments during the course of the last twenty years have resulted in considerable improvements in the main cold forming properties of sheet and strip. The chief demand made of manufacturers of cold-rolled flat products is for sheet and strip which has been fully annealed and rerolled for good cold formability. Because of the various stresses on materials in the different forming processes, it is difficult to make a reliable prediction from test results about the behaviour of the material during actual forming operations. The new edition of sheet 1 of DIN 1623 prescribes the conventional test methods and their results as regards ensuring cold formability. The Standard does not include the additional means of using numerical values for vertical anisotropy r, work hardening exponent n and the maximum drawing ratio (ßo)_max.     

Urokinase-type plasminogen activator receptor reversibly dissociates from complement receptor type 3 (alpha M beta 2' CD11b/CD18) during neutrophil polarization

Previous studies have shown that the leukocyte integrin CR3 (CD11b/CD18) is physically associated with the urokinase-type plasminogen activator receptor (uPAR;CD87), a glycosyl-phosphatidylinositol (GPI)-linked protein, in resting neutrophil membranes. We now show that uPAR-to-CR3 interactions are reversible, correlating with cell shape. Neutrophils were first labeled with fluorescein conjugates of anti-CR3 F(ab')2 fragments followed by capping using a second-step F(ab')2 directed against murine F(ab')2s. Cells were then probed using rhodamine-conjugated anti-uPAR F(ab')2s. Although uPAR co-caps with CR3 on resting cells, uPAR was found to dissociate or "uncap" coincident with spontaneous cell polarization for migration. CR3 caps transformed into uropods while uPAR accumulated at lamellipodia of polarized cells. Capping was unnecessary for the observed distribution of CR3 and uPAR since the anti-CR3 and anti-uPAR F(ab')2s traffic to the uropod and lamellipodium, respectively, during polarization of uncapped cells. These receptors reassociate when cells return to a spherical morphology. In contrast to uPAR, Fc gamma RIIIB did not dissociate from CR3 caps during cell polarization. Resonance energy transfer (RET) microscopy was used to image the spatial distribution of RET and to follow the kinetics of association and dissociation. Initial levels of RET dramatically fell during cell polarization, but did not change on cells fixed with paraformaldehyde. Receptor reassociation was a biphasic process with initial reassociation about the perimeter of a cap, followed by a plateau and a slower rise in RET within a cap. We suggest that cells regulate receptor-receptor associations depending upon their physiologic activities.     

Heavy-duty liquid detergent

A highly concentrated liquid detergent was developed. For the consumer, the quality and costs of this detergent correspond to the light-duty washing powders, The new detergent can be used in washing machines as well as for hand-washing. For the consumer, the liquid product is ready for immediate use, gives no solubility problems in hte washing bath and saves both time and energy by surfactants and containing an enzyme, the liquid detergent is activated for strain removal. It is recommended especially for such strains as collar soil, blood, grass and many kinds of food strains. For the best result, the instruction advises the application of "Flytande Tvättmedel" directly on visible stains in a prespotting fashion.     

BODIPY-Based Photosensitizers as Potential Anticancer and Antibacterial Agents: Role of the Positive Charge and the Heavy Atom Effect

Boron-dipyrromethene derivatives, including cationic and iodinated analogs, were obtained and subjected to physicochemical and in vitro photodynamic activity studies. Iodinated derivatives revealed a substantial heavy atom effect manifested by a bathochromic shift of the absorption band by about 30 nm and fluorescence intensity reduced by about 30–35 times, compared to that obtained for non-iodinated ones. In consequence, singlet oxygen generation significantly increased with Φ_Δ values in the range 0.69–0.97. The in vitro photodynamic activity was evaluated on Gram-positive Staphylococcus aureus, Gram-negative Escherichia coli, and on human androgen-sensitive prostate adenocarcinoma cells (LNCaP). The novel cationic, iodinated BODIPY, demonstrated the highest activity toward all studied cells. An excellent cytotoxic effect was found against LNCaP cells with an IC₅₀ value of 19.3 nM, whereas the viability of S. aureus was reduced by >5.6 log₁₀ at 0.25 μM concentration and by >5.3 log₁₀ in the case of E. coli at 5 μM. Thus, this analog seems to be a very promising candidate for the application in both anticancer and antimicrobial photodynamic therapy.     

Six-color segmentation of multicolor images in the infection studies of Listeria monocytogenes

Multiple immunofluorescent staining is a powerful strategy for visualizing the spatial and temporal relationship between antigens, cell populations, and tissue components in histological sections. To segment different cell populations from the multicolor image generated by immunostaining based on color addition theory, a systems approach is proposed for automatic segmentation of six colors. After image acquisition and processing, images are automatically segmented with the proposed approach and six-pseudo channels for individual or colocalized fluorescent dye are generated to distinguish different cell types. The principle of this approach is the classification of each pixel into one of six colors (red, green, blue, yellow, magenta, and cyan) by choosing the minimal angular deviation between the RGB vector of the given pixel and six classically defined edge vectors. In the present infection studies of Listeria monocytogenes, the new multicolor staining methods based on the color addition were applied and the proposed color segmentation was performed for multicolor analysis. Multicolor analysis was accomplished to study the migration and interaction of Listeria and different cell subpopulations such as CD4CD25 double positive T regulatory cells; we also visualized simultaneously the B cells, T cells, dendritic cells, macrophages, and Listeria in another experiment. After Listeria infection, ERTR9 macrophages and dendritic cells formed cluster with Listeria in the infection loci. The principle of color addition and the systems approach for segmentation may be widely applicable in infection and immunity studies requiring multicolor imaging and analysis. This approach can also be applied for image analysis in the multicolor in vivo imaging, multicolor FISH or karyotyping or other studies requiring multicolor analysis.