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间接竞争ELISA方法用于脱落酸的检测 总被引:1,自引:0,他引:1
该文研制了植物激素脱落酸(ABA)间接竞争酶联免疫试剂盒,该试剂盒可对ABA进行快速检测.文中成功制备了脱落酸和牛血清蛋白的交联物(ABA-BSA),并对交联物的浓度、抗体稀释度、酶标二抗稀释度对实验的影响进行了考察.结果表明,该试剂盒具有高灵敏性,最低检出浓度为1.38 ng/mL,在10ns/mL~1 000 ng/mL之间,吸光度与浓度的对数呈良好的线性关系.样品添加回收率在95.98%~102.25%之间,平均回收率为100.18%,变异系数(CV)在1.27%~4.68%之间,平均变异系数为3.18%. 相似文献
997.
As an important technology for predictive maintenance, failure prognosis has attracted more and more attentions in recent
years. Real-time reliability prediction is one effective solution to failure prognosis. Considering a dynamic system that
is composed of normal, deteriorating and unreliable components, this paper proposes an integrated approach to perform real-time
reliability prediction for such a class of systems. For a deteriorating component, the degradation is modeled by a time-varying
fault process which is a linear or approximately linear function of time. The behavior of an unreliable component is described
by a random variable which has two possible values corresponding to the operating and malfunction conditions of this component.
The whole proposed approach contains three algorithms. A modified interacting multiple model particle filter is adopted to
estimate the dynamic system’s state variables and the unmeasurable time-varying fault. An exponential smoothing algorithm
named the Holt’s method is used to predict the fault process. In the end, the system’s reliability is predicted in real time
by use of the Monte Carlo strategy. The proposed approach can effectively predict the impending failure of a dynamic system,
which is verified by computer simulations based on a three-vessel water tank system. 相似文献
998.
HUANG Ru WU HanMing KANG JinFeng XIAO DeYuan SHI XueLong AN Xia TIAN Yu WANG RunSheng ZHANG LiangLiang ZHANG Xing & WANG YangYuan 《中国科学F辑(英文版)》2009,(9):1491-1533
It is predicted that CMOS technology will probably enter into 22 nm node around 2012. Scaling of CMOS logic technology from 32 to 22 nm node meets more critical issues and needs some significant changes of the technology, as well as integration of the advanced processes. This paper will review the key processing technologies which can be potentially integrated into 22 nm and beyond technology nodes, including double patterning technology with high NA water immersion lithography and EUV lithography, new devi... 相似文献
999.
This paper investigates the controllability of multi-agent systems based on agreement protocols. First, for a group of single-integrator
agents, the controllability is studied in a unified framework for both networks with leader-following structure and networks
with undirected graph. Some new necessary/sufficient conditions for the controllability of networks of single-integrator agents
are established. Second, we prove that, under the same topology and same prescribed leaders, a network of high-order dynamic
agents is completely controllable if and only if so is a network of single-integrator agents. Third, how the selection of
leaders and the coupling weights of graphs affect the controllability is analyzed. Finally, some numerical simulations are
presented to demonstrate the effectiveness of the theoretical results. 相似文献
1000.
该文介绍了随机决策树分类模型及如何启发式选择随机决策树的深度及棵树,通过实验证明了该算法的有效性和高效性。 相似文献