全文获取类型
收费全文 | 1247篇 |
免费 | 76篇 |
国内免费 | 2篇 |
专业分类
电工技术 | 9篇 |
综合类 | 1篇 |
化学工业 | 289篇 |
金属工艺 | 32篇 |
机械仪表 | 20篇 |
建筑科学 | 60篇 |
矿业工程 | 2篇 |
能源动力 | 42篇 |
轻工业 | 77篇 |
水利工程 | 4篇 |
石油天然气 | 1篇 |
无线电 | 64篇 |
一般工业技术 | 291篇 |
冶金工业 | 224篇 |
原子能技术 | 6篇 |
自动化技术 | 203篇 |
出版年
2024年 | 4篇 |
2023年 | 31篇 |
2022年 | 41篇 |
2021年 | 58篇 |
2020年 | 42篇 |
2019年 | 47篇 |
2018年 | 53篇 |
2017年 | 50篇 |
2016年 | 39篇 |
2015年 | 33篇 |
2014年 | 38篇 |
2013年 | 85篇 |
2012年 | 65篇 |
2011年 | 57篇 |
2010年 | 53篇 |
2009年 | 58篇 |
2008年 | 61篇 |
2007年 | 40篇 |
2006年 | 41篇 |
2005年 | 24篇 |
2004年 | 24篇 |
2003年 | 24篇 |
2002年 | 21篇 |
2001年 | 13篇 |
2000年 | 16篇 |
1999年 | 9篇 |
1998年 | 56篇 |
1997年 | 32篇 |
1996年 | 34篇 |
1995年 | 15篇 |
1994年 | 11篇 |
1993年 | 16篇 |
1992年 | 17篇 |
1991年 | 5篇 |
1990年 | 6篇 |
1989年 | 8篇 |
1988年 | 6篇 |
1987年 | 5篇 |
1985年 | 4篇 |
1984年 | 5篇 |
1983年 | 4篇 |
1982年 | 5篇 |
1981年 | 7篇 |
1979年 | 6篇 |
1978年 | 5篇 |
1976年 | 12篇 |
1969年 | 3篇 |
1957年 | 3篇 |
1954年 | 3篇 |
1943年 | 4篇 |
排序方式: 共有1325条查询结果,搜索用时 15 毫秒
191.
Andre Gross Jonas Schoendube Stefan Zimmermann Maximilian Steeb Roland Zengerle Peter Koltay 《International journal of molecular sciences》2015,16(8):16897-16919
The handling of single cells is of great importance in applications such as cell line development or single-cell analysis, e.g., for cancer research or for emerging diagnostic methods. This review provides an overview of technologies that are currently used or in development to isolate single cells for subsequent single-cell analysis. Data from a dedicated online market survey conducted to identify the most relevant technologies, presented here for the first time, shows that FACS (fluorescence activated cell sorting) respectively Flow cytometry (33% usage), laser microdissection (17%), manual cell picking (17%), random seeding/dilution (15%), and microfluidics/lab-on-a-chip devices (12%) are currently the most frequently used technologies. These most prominent technologies are described in detail and key performance factors are discussed. The survey data indicates a further increasing interest in single-cell isolation tools for the coming years. Additionally, a worldwide patent search was performed to screen for emerging technologies that might become relevant in the future. In total 179 patents were found, out of which 25 were evaluated by screening the title and abstract to be relevant to the field. 相似文献
192.
Anna Sib Tobias M. Milzarek Alexander Herrmann Dr. Lila Oubraham Jonas I. Müller Prof. Dr. Andreas Pichlmair Prof. Dr. Ruth Brack-Werner Prof. Dr. Tobias A. M. Gulder 《Chembiochem : a European journal of chemical biology》2020,21(4):492-495
Sorbicillinoids are fungal polyketides characterized by highly complex and diverse molecular structures, with considerable stereochemical intricacy combined with a high degree of oxygenation. Many sorbicillinoids possess promising biological activities. An interesting member of this natural product family is sorbicatechol A, which is reported to have antiviral activity, particularly against influenza A virus (H1N1). Through a straightforward, one-pot chemoenzymatic approach with recently developed oxidoreductase SorbC, the characteristic bicyclo[2.2.2]octane core of sorbicatechol is structurally diversified by variation of its natural 2-methoxyphenol substituent. This facilitates the preparation of a focused library of structural analogues bearing substituted aromatic systems, alkanes, heterocycles, and ethers. Fast access to this structural diversity provides an opportunity to explore the antiviral potential of the sorbicatechol family. 相似文献
193.
Nylin Magnus Johansson Westberg Jimmy Lundberg Jonas 《Cognition, Technology & Work》2022,24(2):261-273
Cognition, Technology & Work - Lack of support for handling a reduction of autonomy in a highly autonomous automation may lead to a stressful situation for a human when forced to take over. We... 相似文献
194.
Aurita Butkevičiūtė Darius Kviklys Kristina Zymonė Raimondas Raudonis Jonas Viškelis 《International Journal of Food Properties》2018,21(1):1716-1727
A new technique for the rapid detection and analysis of triterpenic compounds in apple extracts using HPLC was developed and validated. The main advantage of this technique is the short duration of the analysis – this makes this technique superior to others currently applied for the routine HPLC analysis of triterpenic compounds. The developed, optimized, and validated technique was used for the evaluation of triterpenic compounds in samples of different cultivars of apples, their peels, and flesh. In total, four triterpenic compounds were isolated and identified. Ursolic acid was the dominant compound in all the tested apple samples. The highest amounts of triterpenic compounds were detected in the peels of the ‘Lodel’ apple cultivar, and thus apples of this cultivar may be potentially useful for the isolation of individual compounds and the production of functional food and dietary supplements. 相似文献
195.
Mirta Galesic Henrik Olsson Jonas Dalege Tamara van der Does Daniel L. Stein 《Journal of the Royal Society Interface》2021,18(176)
Belief change and spread have been studied in many disciplines—from psychology, sociology, economics and philosophy, to biology, computer science and statistical physics—but we still do not have a firm grasp on why some beliefs change more easily and spread faster than others. To fully capture the complex social-cognitive system that gives rise to belief dynamics, we first review insights about structural components and processes of belief dynamics studied within different disciplines. We then outline a unifying quantitative framework that enables theoretical and empirical comparisons of different belief dynamic models. This framework uses a statistical physics formalism, grounded in cognitive and social theory, as well as empirical observations. We show how this framework can be used to integrate extant knowledge and develop a more comprehensive understanding of belief dynamics. 相似文献
196.
OBJECTIVE: To compare the results of cardiac output measurements obtained by lithium dilution and thermodilution. DESIGN: Case series, observational study. SETTING: High-dependency postoperative unit and intensive care unit of a teaching hospital. PATIENTS: Forty patients were studied. Thirty-four patients had undergoing heart surgery requiring cardiopulmonary bypass within the previous 2 days; the diagnoses in the other patients were myocardial infarct (n = 2), septicemia (n = 2), adult respiratory distress syndrome, and pericardectomy. INTERVENTIONS: Cardiac output was measured five times in each patient, using lithium dilution (single measurement) and bolus thermodilution (series of three to six measurements according to standard clinical practice, taking the average of the closest three). In a subgroup of 14 patients, cardiac output was also measured using "continuous thermodilution." MEASUREMENTS AND MAIN RESULTS: Comparing lithium dilution with bolus thermodilution, the mean of the differences (lithium dilution-thermodilution) was -0.25 +/- 0.46 [SD] L/min. Linear regression analysis gave y = 0.31 + 0.89x (r2 = .94) for lithium dilution vs. thermodilution. CONCLUSIONS: The overall agreement between the two methods was good. The variability of the thermodilution measurements was greater than that of the lithium dilution measurements. The lithium dilution method is at least as accurate as bolus thermodilution and, since pulmonary artery catheterization is not needed, it has the advantages of being safe and quick to perform. 相似文献
197.
Lundgren Jonas Klarbring Anders Lundgren Jan-Erik Thore Carl-Johan 《Structural and Multidisciplinary Optimization》2019,60(6):2295-2303
Structural and Multidisciplinary Optimization - We consider a model for density-based topology optimization (TO) of stationary heat transfer problems with design-dependent internal convection in 3D... 相似文献
198.
199.
Stefan Bleher Jonas Buck Christian Muhl Sandro Sieber Sabine Barnert Dominik Witzigmann Jrg Huwyler Matthias Barz Regine Süss 《Small (Weinheim an der Bergstrasse, Germany)》2019,15(50)
Circulation lifetime is a crucial parameter for a successful therapy with nanoparticles. Reduction and alteration of opsonization profiles by surface modification of nanoparticles is the main strategy to achieve this objective. In clinical settings, PEGylation is the most relevant strategy to enhance blood circulation, yet it has drawbacks, including hypersensitivity reactions in some patients treated with PEGylated nanoparticles, which fuel the search for alternative strategies. In this work, lipopolysarcosine derivatives (BA‐pSar, bisalkyl polysarcosine) with precise chain lengths and low polydispersity indices are synthesized, characterized, and incorporated into the bilayer of preformed liposomes via a post insertion technique. Successful incorporation of BA‐pSar can be realized in a clinically relevant liposomal formulation. Furthermore, BA‐pSar provides excellent surface charge shielding potential for charged liposomes and renders their surface neutral. Pharmacokinetic investigations in a zebrafish model show enhanced circulation properties and reduction in macrophage recognition, matching the behavior of PEGylated liposomes. Moreover, complement activation, which is a key factor in hypersensitivity reactions caused by PEGylated liposomes, can be reduced by modifying the surface of liposomes with an acetylated BA‐pSar derivative. Hence, this study presents an alternative surface modification strategy with similar benefits as the established PEGylation of nanoparticles, but with the potential of reducing its drawbacks. 相似文献
200.
Marlon Wesley Machado Cunico Dalton Alexandre Kai Patrick Medeiros Cavalheiro Jonas de Carvalho 《Virtual and Physical Prototyping》2019,14(1):37-52
In recent years, additive manufacturing technologies have been playing an important role in the global economy. Consequently, low-cost 3D printers rose in the domestic environment, accelerating the development of startup companies and new market segments. Nevertheless, with regard to 3D printing based on fused filament fabrication, several challenges still need to be overcome, such as those related to surface finishing and mechanical strength. Moreover, 3D printing in the domestic environment also generates untreated plastic waste, which can cause environmental problems. For these reasons, the main goal of this work is to introduce and characterise 3D printing surface finishing post-processing using recycled plastic waste. As the main results of this work, the proposed recycling process was confirmed to improve object properties. Whereas surface roughness was reduced from 27 to 3?µm, while mechanical strength was increased in 20 times. The application of recycled material for chemical welding was also seen to be feasible. 相似文献