Study of proteolysis during the processing of a dry fermented pork sausage

Dry pork sausage was formulated, fermented and dried for 41 days. Gross composition was determined and proteolysis was monitored by assaying the following N fractions: water-soluble nitrogen (WSN), salt-soluble nitrogen (SSN), ultrafiltration (UF) permeates of WSN and SSN, phosphotungstic acid (PTA)-soluble and -insoluble fractions and free amino acids. Meat and sausages, water- and salt-insoluble components, UF-retentates of WSN and SSN were assessed by SDS-PAGE at different stages of ripening.

The amount of WSN, WSN permeate, PTA-soluble N and free amino acids increased during processing, while the SSN and PTA-insoluble N decreased. The electrophoretic studies demonstrated that proteolysis of the heavy myosin chain, -actinin and actin was most prominent. The increased insolubility of meat proteins was confirmed by SDS-PAGE.     

Biohydrogen and 5-aminolevulinic acid production from waste barley by Rhodobacter sphaeroides O.U.001 in a biorefinery concept

The production of biohydrogen and 5-aminolevulinic acid (5-ALA) by Rhodobacter sphaeroides O.U.001 was investigated in a biorefinery concept. Waste barley was used as a substrate after acid hydrolysis. The hydrolysate was analyzed in terms of its total simple sugar, organic acid, ammonium, element and total phenol contents. Four different growth media having 5 g/L, 7 g/L, 9 g/L and 11 g/L sugar content were prepared using the waste barley hydrolysate to produce biohydrogen and 5-ALA. The increased sugar concentrations resulted in higher cell density and hydrogen accumulation. Accordingly, the highest cell density (OD₆₆₀: 1.78) and hydrogen production (0.4 L H₂/L culture) were observed in the 11 g/L sugar-containing medium. A 67.4 μM 5-ALA was produced upon vitamin B₁₂ and levulinic acid additions. These results showed that waste barley can be used as a substrate for R. sphaeroides for biohydrogen and 5-ALA production within a biorefinery concept.     

Stand-alone operation of an alkaline water electrolyser fed by wind and photovoltaic systems

Over the last few years, hydrogen technologies have established themselves as key enablers in the medium and long-term development of a new energy model that offers greater sustainability and independence than the present-day one. In this respect, the integration of water electrolysis with renewable energy-based systems can play an important part in the large-scale production of sustainable hydrogen. This paper reports on the complete experimental characterisation of a 1 Nm³ h⁻¹ alkaline water electrolyser located in the Public University of Navarre (UPNa). Specifically, a study was made of the electrical performance, hydrogen production rate, purity of the gases generated and energy efficiency, for a range of operating currents (40–120 A), temperatures (35–65 °C) and pressures (5–25 bar). Additionally, an experimental study was conducted on the electrolyser operation under conditions that are characteristic of a stand-alone wind power and PV-based renewable energy system, installed at the UPNa. The results obtained for the wind power and PV emulations showed that the electrolyser performed correctly, with regard to balance of plant and its principal electrochemical characteristics. Furthermore, the mean energy efficiency of the electrolyser was 77.7% for the wind power emulation, and 78.6% for the PV emulation on a day with stable irradiance, and 78.1% on a day with highly variable irradiance (day with scattered clouds).     

A methodology for the efficient computer representation of dynamic power systems: Application to wind parks

This contribution presents a methodology to efficiently obtain the numerical and computer solution of dynamic power systems with high penetration of wind turbines. Due to the excessive computational load required to solve the abc models that represent the behavior of the wind turbines, a parallel processing scheme is proposed to enhance the solution of the overall system. Case studies are presented which demonstrate the effectiveness and applications of the proposed methodology. Copyright © 2012 John Wiley & Sons, Ltd.     

District heating and cooling for business buildings in Madrid

Energy needs for heating and cooling in Spain are of paramount interest in the context of the European roadmap to a decarbonized environment; because of that, it is highly desirable that more examples of district heating and cooling networks are developed. The present work evaluates the implementation of one of them into the climatic environment of Madrid. It consists on a complex of business office buildings with a total useful surface of 50,000 m², linked with heating and cooling rings of 1 km of loop length. Basic energy needs of buildings lead to the following design values: 1.7 MW of electricity, 1.3 MW of heating and 2 MW of cooling. They will be supplied by the trigeneration plant here proposed, which relies on an internal combustion engine.The high demand of cooling for air conditioning makes the dimensioning of the engine critical because of the large differences between the heat demand for summer and the one for winter. If the total amount of the cooling demand is covered with an absorption chiller, the heat demand during the summer reaches about 5 MW. In consequence, a critical decision has to be taken relative to the way the cooling demand is attended: with an absorption chiller (single or double effect) or with a conventional chiller powered by electricity. Applying the criteria developed in the present work, which are focused on maximum primary energy reduction, the fraction of the cooling demand to be met with each technology is determined as a function of the engine nominal power, on the grounds of the instantaneous demand.The high cooling demand during the summer season suggests the inclusion of a thermal solar collector field, to be used for complementing the waste heat rejection from the engine to drive the absorption chiller. During the winter, the heat provided by the solar field could be applied in attending a fraction of the heating demand. Thus a hybrid Trigeneration Plant is introduced. This way, over sizing of the engine can be avoided, as the electric demand is small.The analysis is based on the solution of energy and mass balance equations for a trigeneration plant. Monthly demands and environmental conditions (ambient temperature and solar irradiance) are introduced as input data into the model. Monthly and annual primary energy consumption and CO₂ emission reductions are obtained as outputs. Economical data, such as fuel and operating costs, electricity prices, tariffs and subsidies are considered in order to optimize the size of the plant in terms of its payback period.     

Development and Comparison of Two Fast Surrogate Models for Urban Pluvial Flood Simulations

Detailed full hydrodynamic 1D-2D dual drainage models are a well-established approach to simulate urban pluvial floods. However, despite modelling advances and increasing computational power, this approach remains unsuitable for many real time applications. We propose and test two computationally efficient surrogate models. The first approach links a detailed 1D sewer model to a GIS-based overland flood network. For the second approach, we developed a conceptual sewer and flood model using data-driven and physically based structures, and coupled the model to pre-simulated flood maps. The city of Ghent (Belgium) is used as a test case. Both surrogate models can provide comparable results to the original model in terms of peak surface flood volumes and maximum flood extent and depth maps, with a significant reduction in computing time.     

Methodology to Optimize Rainwater Tank-sizing and Cluster Configuration for a Group of Buildings

Water Resources Management - The behavior of a rainwater harvesting system depends on some variables that cannot be controlled, such as rainfall, building roof size and water consumption. The...     

禁用含金属染料的新情况及新染料的开发

本文阐述了欧盟发出的禁用和限制含金属染料的新情况，介绍了取代禁用和限制含金属染料的新型染料。     

Oxidation of cysteine and glutathione by soluble polymeric MnO2

The kinetics of reduction of soluble polymeric MnO2 by cysteine and glutathione has been studied in the pH range of 4.0-9.0. The concentration of thiols was varied between 1 and 2 mM, while the MnO2 concentration was varied between 2 and 12 microM. In this pH range, the reaction products were identified as Mn(II) and the corresponding disulfides (cystine and glutathione disulfide). Cysteic or cysteine sulfonic acid was formed only when pH < 2. Experimental data indicate that the rate law over the pH range of 4-9 is first-order in both MnO2 and thiol concentration. Eyring plots for both thiols reacting with MnO2 indicate that the reaction is associative (deltaS(double dagger) approximately -160 J mol(-1) K(-1)) and proceeds via an inner-sphere redox process. The reaction proceeds via the formation of two different inner-sphere complexes [triple bond]Mn(IV)SR- and [triple bond]Mn(IV)SR and their further reaction to products. Both surface species are linked to each other via acid-base equilibria, and the rate constant decreases as pH increases. The presence of two ligand surface species is determined using surface complexation modeling. A reaction mechanism in agreement with the experimental results is proposed.     

Kinetic behaviour and stability of Escherichia coli ATCC27257 alkaline phosphatase immobilised in soil humates

Alkaline phosphatase (EC 3.1.3.1) extracted from Escherichia coli ATCC27257 was immobilised by co‐flocculation with soil humates in the presence of Ca²⁺. The effects of time, temperature, pH and concentration of enzyme and support on immobilisation were studied. Between 58 and 92% of the added phosphatase was strongly bound to the humates, depending on the conditions of immobilisation used. Some characteristics of the humate–phosphatase complexes and of the free enzyme were compared. The enzymatic complexes showed values of K_m (2.22 mM ) and activation energy (33.4 kJ mol^?1) similar to those of the free enzyme (2.00 mM and 27.6 kJ mol^?1). The pH/activity profiles revealed no change in terms of shape or optimum pH (10.5) upon immobilisation of alkaline phosphatase. However, the immobilised enzyme showed maximal activity in the range of 80–100 °C, while the free enzyme had its highest activity at 60 °C. The thermal stability of alkaline phosphatase was enhanced by complexation to the soil humates. © 2003 Society of Chemical Industry