A Single Injection of NTG-101 Reduces the Expression of Pain-Related Neurotrophins in a Canine Model of Degenerative Disc Disease

Background: Tissue sources of pain emanating from degenerative discs remains incompletely understood. Canine intervertebral discs (IVDs) were needle puncture injured, 4-weeks later injected with either phosphate-buffered saline (PBS) or NTG-101, harvested after an additional fourteen weeks and then histologically evaluated for the expression of NGFr, BDNF, TrkB and CALCRL proteins. Quantification was performed using the HALO automated cell-counting scoring platform. Immunohistochemical analysis was also performed on human IVD tissue samples obtained from spinal surgery. Immunohistochemical analysis and quantification of neurotrophins and neuropeptides was performed using an in vivo canine model of degenerative disc disease and human degenerative disc tissue sections. Discs injected with NTG-101 showed significantly lower levels of Nerve Growth Factor receptor (NGFr/TrkA, p = 0.0001), BDNF (p = 0.009), TrkB (p = 0.002) and CALCRL (p = 0.008) relative to PBS injections. Human IVD tissue obtained from spinal surgery due to painful DDD show robust expression of NGFr, BDNF, TrkB and CALCRL proteins. A single intradiscal injection of NTG-101 significantly inhibits the expression of NGFr, BDNF, TrkB and CALCRL proteins in degenerative canine IVDs. These results strongly suggest that NTG-101 inhibits the development of neurotrophins that are strongly associated with painful degenerative disc disease and may have profound effects upon the management of patients living with discogenic pain.     

HSPA4 Is a Biomarker of Placenta Accreta and Enhances the Angiogenesis Ability of Vessel Endothelial Cells

Placenta accreta spectrum (PAS) accounts for 7% of maternal mortality and is associated with intraoperative and postoperative morbidity caused by massive blood loss, infection, and adjacent organ damage. The aims of this study were to identify the protein biomarkers of PAS and to further explore their pathogenetic roles in PAS. For this purpose, we collected five placentas from pregnant subjects with PAS complications and another five placentas from normal pregnancy (NP) cases. Then, we enriched protein samples by specifically isolating the trophoblast villous, deeply invading into the uterine muscle layer in the PAS patients. Next, fluorescence-based two-dimensional difference gel electrophoresis (2D-DIGE) and MALDI-TOF/MS were used to identify the proteins differentially abundant between PAS and NP placenta tissues. As a result, nineteen spots were determined as differentially abundant proteins, ten and nine of which were more abundant in PAS and NP placenta tissues, respectively. Then, specific validation with western blot assay and immunohisto/cytochemistry (IHC) assay confirmed that heat shock 70 kDa protein 4 (HSPA4) and chorionic somatomammotropin hormone (CSH) were PAS protein biomarkers. Further tube formation assays demonstrated that HSPA4 promoted the in vitro angiogenesis ability of vessel endothelial cells, which is consistent with the in vivo scenario of PAS complications. In this study, we not only identified PAS protein biomarkers but also connected the promoted angiogenesis with placenta invasion, investigating the pathogenetic mechanism of PAS.     

莱钢1号1880m3高炉装料制度的探索

对莱钢1号1 880m3高炉装料制度的调整经验进行了总结.主要经验是:采用大矿角、大焦角、大角差的装料制度,炉料偏析小,炉况稳定性好,煤气利用率高.     

压电引信传感器设计技术

本文通过对武器系统使用条件和触发引信技术要求的分析,提出了压电引信方案,并系统地介绍了该引信传感器的工作原理、结构设计及调试方法。通过性能实验,证明这种压电引信传感器瞬发度高,万向性好,满足了技术要求,是种较好的快速响应万向性引信用传感器。该传感器也可用于各种三维冲击,振动测量中。     

P(MMA-AN)/TiO2复合粒子的制备及光催化性能

采用原位乳液聚合方法制备了基于共价键结合的P(MMA-AN)/TiO2复合粒子,红外光谱和热失重分析结果表明P(MMA-AN)在TiO2表面接枝率为15.9%。以甲基橙溶液为目标污染物,研究了P(MMA-AN)/TiO2复合粒子和TiO2对甲基橙的吸附和光催化降解甲基橙溶液的性能,结果表明:两种粒子对甲基橙的吸附都很少;在相同时间内,P(MMA-AN)/TiO2复合粒子对甲基橙溶液的降解性能优于TiO2,紫外光照180 min后,P(MMA-AN)/TiO2复合粒子对甲基橙溶液的降解率达到了88%,而TiO2仅为74%。     

Modulating lateral strain in GaN-based epitaxial layers by patterning sapphire substrates with aligned carbon nanotube films

A novel carbon nanotube-patterned sapphire substrate (CPSS) has been utilized for the growth of GaN material and fabrication of a InGaN/GaN light emitting diode (LED) by metal-organic vapor phase epitaxy. Different lateral strain distributions and stress reductions were observed in a GaN thin film on CPSS compared with those on a conventional sapphire substrate. Nanoheteroepitaxy induced by small size nucleation islands of about 50 nm is ascribed to this significant strain modulation. The crystalline quality of the GaN thin film was also improved, as illustrated by X-ray diffraction. Performances of 1 mm × 1 mm LEDs on CPSS were also enhanced, with an operational power increase of 37.5% and higher saturation current.      

大起大落重又崛起的APS

ＡＰＳ单反相机在１９９７年问世，ＡＰＳ表现出优良的画面质量，使得消费者在对它冷落一段时间之后不得不进行重新评价。另外，提出了ＡＰＳ在中国所遇到问题，如缺少冲扩店，胶卷价格贵等。     

Therapy for cerebral ischemic injury with erythropoietin-containing nanoparticles

In this study we prepared nanocarrier of erythropoietin (EPO) and the curative effect of the EPO-containing nanoparticles for periventricular leukomalacia (PVL) model was validated. It was demonstrated that EPO-containing nanoparticles can ameliorate drug-induced liquefaction caused by hypoxia. EPO-containing nanomedicine may open new therapeutic strategies in hypoxia cure.