Proteinase activity in the isolates of Trichomonas vaginalis according to their pathogenicity

Ten axenic isolates of Trichomonas vaginalis were subcutaneously injected to the BALB/c mice in order to assess their pathogenicity by means of so-called "mouse assay" method. All the isolates revealed neutral and acid proteinase activities both in their lysates and in culture media, but the specific activities of both proteinases in the severely pathogenic group were significantly higher than the mildly pathogenic group (p < 0.05). In the SDS-PAGE system in which the electrophoretic gels contained 0.4% gelatin as the substrate, five different banding patterns of trichomonal proteinases were detected, and the patterns were closely related with the pathogenicity of the isolates of T. vaginalis. All five bands might be regarded as cysteine proteinases group in the inhibitor assays. The cytotoxicity of the lysates of T. vaginalis to the target Chinese hamster ovarian (CHO) cell line was also significantly different according to the pathogenicity of the isolates, and generally lower in the lysates treated with cysteine proteinase inhibitors than in the control lysates. In summarizing the results, it might be considered that the proteinases of T. vaginalis showing five electrophoretic banding patterns are closely related with the pathogenicity and cytotoxicity of the isolates of T. vaginalis.     

Sequence-based differentiation of strains in the Mycobacterium avium complex

The complete 16S-23S rDNA internal transcribed spacer (ITS) was sequenced in 35 reference strains of the Mycobacterium avium complex. Twelve distinct ITS sequences were obtained, each of which defined a "sequevar"; a sequevar consists of the strain or strains which have a particular sequence. ITS sequences were identified which corresponded to M. avium (16 strains, four ITS sequevars) and Mycobacterium intracellulare (12 strains, one ITS sequevars). The other seven M. avium complex strains had ITS sequences which varied greatly from those of M. avium and M. intracellulare and from each other. The 16S-23S rDNA ITS was much more variable than 16S rDNA, which is widely used for genus and species identification. Phylogenetic trees based on the ITS were compatible with those based on 16S rDNA but were more detailed and had longer branches. The results of ITS sequencing were consistent with the results of hybridization with M. avium and M. intracellulare probes (Gen-Probe) for 30 of 31 strains tested. Serologic testing correlated poorly with ITS sequencing. Strains with the same sequence were different serovars, and those of the same serovar had different sequences. Sequencing of the 16S-23S rDNA ITS should be useful for species and strain differentiation for a wide variety of bacteria and should be applicable to studies of epidemiology, diagnosis, virulence, and taxonomy.     

A-IMilano apoprotein. Decreased high density lipoprotein cholesterol levels with significant lipoprotein modifications and without clinical atherosclerosis in an Italian family

Significant hypertriglyceridemia with a very marked decrease of high density lipoproteins (HDL)-cholesterol levels (7-14 mg/dl) was detected in three members (father, son, and daughter) of an Italian family. The three affected individuals did not show any clinical signs of atherosclerosis, nor was the atherosclerotic disease significantly present in the family. Lipoprotein lipase and lecithin:cholesterol acyltransferase activites were normal or slightly reduced. Morphological and compositional studies of HDL in the subjects showed a significant enlargement of the lipoprotein particles (approximately 120 vs. approximately 94 A for control HDL) and a concomitant increase in the triglyceride content. Analytical isoelectric focusing of HDL apoproteins provided evidence for multiple isoproteins in the apoprotein(apo)-A-I range, with nine different bands being detected instead of the usual four bands observed in normal subjects. Two-dimensional immunoelectrophoresis against apo-A antiserum indicated a clear reduction of apo-A in the alpha electrophoretic region, with splitting of the protein "peak." The observation in otherwise clinically healthy subjects of hypertriglyceridemia, reduced HDL-cholesterol, and marked apoprotein abnormalities, without a significant incidence of atherosclerotic disease in the family suggests this is a new disease entity in the field of lipoprotein pathology, very probably related to an altered amino acid composition of the apo-A-I protein (see Weisgraber et al. 1980. J. Clin. Invest. 66: 901-907).     

Efficient screen-space approach to high-quality multiscale ambient occlusion

We present a new screen-space ambient occlusion (SSAO) algorithm that improves on the state-of-the-art SSAO methods in both performance and quality. Our method computes ambient occlusion (AO) values at multiple image resolutions and combines them to obtain the final, high-resolution AO value for each image pixel. It produces high-quality AO that includes both high-frequency shadows due to nearby, occluding geometry and low-frequency shadows due to distant geometry. Our approach only needs to use very small sampling kernels at every resolution, thereby achieving high performance without resorting to random sampling. As a consequence, our results do not suffer from noise and excessive blur, which are common of other SSAO methods. Therefore, our method also avoids the expensive, final blur pass commonly used in other SSAO methods. The use of multiple resolutions also helps reduce errors that are caused by SSAO’s inherent lack of visibility checking. Temporal incoherence caused by using coarse resolutions is solved with an optional temporal filtering pass. Our method produces results that are closer to ray-traced solutions than those of any existing SSAO methods, while running at similar or higher frame rates than the fastest ones.     

Grafting of enzymes on collagen films using Woodward''s reagent "K" and a water-soluble carbodiimide derivative

Two new methods of activation were developed to graft enzymes on collegen films. They involved chemical modifications of surface groups of collagen either by Woodward's reagent "K" or by EDC, a water-soluble derivative of carbodiimide. EDC was a better coupling agent and a detailed study was conducted with this agent. It could be used either in a global method of activation and coupling, or in a two-step procedure of activation of collagen, followed by spontaneous coupling of enzyme. All enzymes tested were successfully bound: malate dehydrogenase, lactate dehydrogenase, aspartate aminotransferase, urease, creatine kinase, hexokinase. The influence on the yield of grafted enzyme, of pretreatment of films, time and temperature of EDC activation, concentration of EDC and enzyme, protecting agents was studied. Stability of enzyme activity on storage was greatly increased after grafting. A co-grafted dual system creatine kinase/heoxkinase, was achieved which exhibited a good efficiency. A striking renaturing process at 0-4degreesC after thermal denaturation, was observed with hexokinase.     

Application-Oriented Flow Control: Fundamentals, Algorithms and Fairness

This paper is concerned with flow control and resource allocation problems in computer networks in which real-time applications may have hard quality of service (QoS) requirements. Recent optimal flow control approaches are unable to deal with these problems since QoS utility functions generally do not satisfy the strict concavity condition in real-time applications. For elastic traffic, we show that bandwidth allocations using the existing optimal flow control strategy can be quite unfair. If we consider different QoS requirements among network users, it may be undesirable to allocate bandwidth simply according to the traditional max-min fairness or proportional fairness. Instead, a network should have the ability to allocate bandwidth resources to various users, addressing their real utility requirements. For these reasons, this paper proposes a new distributed flow control algorithm for multiservice networks, where the application's utility is only assumed to be continuously increasing over the available bandwidth. In this, we show that the algorithm converges, and that at convergence, the utility achieved by each application is well balanced in a proportionally (or max-min) fair manner     

Modeling and simulation for a drop-impact analysis of multi-layered printed circuit boards

Multi-layered printed circuit boards (PCBs) contain a multi-layered structure that is suitable for high-speed and high-frequency applications. Hence, they are used extensively in electronic packaging assemblies for high-density applications. However, numerous composite parts and complex material properties of multi-layer PCBs complicate the reliability simulation of PCB model. This paper deals with a finite element analysis intended to describe numerically the behavior of multi-layered multi-materials PCB model (combination of metallic and composite plies) in the drop-impact performance. Through the comparison of physical drop test results, the fully multi-layered model illustrates higher accuracy if compared with that of the traditional simplified isotropic model and orthotropic model. The effects of material properties for the multi-layer PCB under drop-impact shock have also been investigated.     

Friendship Jealousy in Young Adolescents: Individual Differences and Links to Sex, Self-Esteem, Aggression, and Social Adjustment.

Children's vulnerability to jealousy surrounding their best friends was explored in 2 studies. Study 1 involved 94 adolescents who reported on their friendship jealousy on a newly created measure. Results indicated that the jealousy measure had sound psychometric properties and produced individual differences that were robust over time and free from socially desirable responding. As expected, girls and adolescents with low self-worth reported the greatest friendship jealousy. Study 2 involved 399 young adolescents and extended the measurement of self-report jealousy to a broader age range. In addition, Study 2 included assessments of jealousy provided by friends and other peers. Self- and peer-reported jealousy were only modestly associated and had somewhat distinct correlates. Structural modeling revealed that young adolescents' reputation for friendship jealousy was linked to behaving aggressively and to broader peer adjustment difficulties. Both self- and peer-reported jealousy contributed to loneliness. (PsycINFO Database Record (c) 2010 APA, all rights reserved)