Residential Mobility,Locational Disadvantage And Spatial Inequality In Australian Cities

The purpose of this presentation is to contribute to the debate around the nature of spatial inequality in Australian cities. It takes issue with the view that the extensive spatial form of Australian cities is a major element in the transmission of social disadvantage – a view which has gained currency in recent urban policy debates about “sprawl” vs consolidation. The assumption (in some quarters) that households located at, or moving to, the newly developing urban periphery, have been forced there through housing market processes in which they have had little choice; and further, that once there the lack of proximate resources, particularly in social infrastructure, reinforce the already disadvantaged social and economic circumstances of these households does not stand scrutiny. The coincidence of locational disadvantage and social disadvantage has been assumed rather than demonstrated, seemingly in an attempt to bolster arguments about the need to alter the predominant form of urban growth – outward extension of low density suburbia.     

Simulation of the deflected cutting tool trajectory in complex surface milling

Since industry is rapidly developing, either locally or globally, manufacturers witness harder challenges due to the growing competitivity. This urges them to better consider the four factors linked to production and output: quality, quantity, cost and price, quality being of course the most important factor which constitutes their main concern. Efforts will be concentrated??in this research??on improving the quality and securing more accuracy for a machined surface in ball-end milling. Quality and precision are two essential criteria in industrial milling. However, milling errors and imperfections, due mainly to the cutting tool deflection, hinder the full achieving of these targets. Our task, all along this paper, consists in studying and realizing the simulation of the deflected cutting tool trajectory, by using the methods which are available. In a future stage, and in the frame of a deeper research, the simulation process will help to carry out the correction and the compensation of the errors resulting from the tool deflection. The corrected trajectory which is obtained by the method mirror will be sent to the machine. To achieve this goal, the next process consists??as a first step??in selecting a model of cutting forces for a ball-end mill. This allows to define??later on??the behavior of this tool, and the emergence of three methods namely the analytical model, the finite elements method, and the experimental method. It is possible to tackle the cutting forces simulation, all along the tool trajectory, while this latter is carrying out the sweeping of the part to be machined in milling and taking into consideration the cutting conditions, as well as the geography of the workpiece. A simulation of the deflected cutting tool trajectory dependent on the cutting forces has been realized.     

Switched-capacitor high-speed emulator for real-time fault location in electrical power systems

This research presents a discrete-time transmission line model based on the propagation of travelling waves. In this approach, the transmission line is emulated by means of many interconnected unit delay cells implemented with switched-capacitor (SC) circuits. The accuracy and limitations of this method is compared to existing transconductance–capacitor solutions and is evaluated in the frame of a novel power network fault location method based on the electromagnetic time-reversal principle. The impact of the non-ideal effects associated to analog CMOS SC circuits, such as amplifier finite gain, offset and switch charge injection is evaluated in the same context. A possible application of the model for the simulation of interconnected or multi-conductor lines is also discussed. After an AMS 0.35 µm process implementation, it is shown that the present method allows a fault location within 1% resolution and is a hundred times faster than nowadays digital solutions. This speed improvement allows a fault location within 160 ms, making thus real-time applications realistic.     

Cellular fluorescent indicators and voltage/ion probe reader (VIPR) tools for ion channel and receptor drug discovery

High throughput functional assays are increasingly relied upon to generate early and novel discovery leads for drug development. Ion transport proteins including channels, transporters, and pumps play central roles in cellular bioenergetics, excitability, and a multitude of other biological functions. Facile, robust methods for detecting ion transport activity in both native and heterologous systems is desirable for rapid functional analysis and drug discovery for these difficult but important targets. Here we discuss cell-compatible fluorescent probes, functional assays, and VIPR instrumentation that are used to monitor real-time target activity and screen large chemical libraries for potent and selective modulators. Advances and issues for both exogenously applied and fluorescent protein probes of cellular membrane potential, Ca2+, Cl-, and pH are addressed. High throughput screening (HTS) compatible, rapid kinetic and fluorescence resonance energy transfer (FRET) assays are emphasized, in particular the use of voltage-sensitive FRET probes to assay ion channel activity in single cells and 96/384-well formats.     

The finite-element deformation modeling of superplastic Al-8090

The deformation behaviors of an Al-Li-Cu alloy (Aluminum 8090) during uniaxial and biaxial stress states were modeled using the nonlinear finite element analysis package ABAQUS 5.8. Two different material models were used for the purpose of comparison and to propose a valid and accurate superplastic material deformation behavior. Using a power law strain hardening model, the material was modeled with either constant or variable properties of strain-rate sensitivity and strain-hardening exponent. Results from the numerical models were compared to experimental results by Chen and Huang, who investigated the uniaxial and biaxial states of stress. Experimental data were primarily used to acquire the needed uniaxial material parameters for the model. The strain-rate sensitivity and strain-hardening exponent were dependent on the strain and strain rates. Comparing the two material models for both stress states indicated that the material model with variable properties better represented the true behavior of the material when compared to the experimental results. For more information, contact Noha M. Hassan, the American University in Cairo, 113 Kasr El Aini St., P.O. Box 2511, Cairo 11511, Egypt; +202-797-5336; fax +202-797-7565; e-mail nhassan@vt.edu.     

Prognostic significance of colony-stimulating factor receptor expression in ipsilateral breast cancer recurrence

The macrophage colony-stimulating factor receptor (CSF-1R), the product of the c-fms proto-oncogene, regulates normal proliferation and differentiation of macrophages and trophoblasts. Recent research found abnormal expression of CSF-1R in human carcinomas of the breast, endometrium, and ovary. Furthermore, activation of CSF-1R by its ligand has been shown to regulate invasiveness and anchorage-independent growth in breast carcinoma cells. To study the significance of CSF-1R expression in breast cancer, we designed a case-controlled immunohistochemical study. We chose 80 patients from a database of 1200 early stage I or II breast cancer patients treated with conservative surgery and radiation therapy. Expression of CSF-1R in the tumors of 40 patients who experienced an ipsilateral breast tumor recurrence (IBTR) as a primary site of relapse were compared with 40 patients who had not experienced an IBTR. The index and control patients were matched by age, clinical stage, nodal status, and follow-up. Paraffin-embedded sections were immunostained with antibodies directed toward CSF-1R. For the CSF-1R antibody, a total of 28 index cases (70%) demonstrated strong staining, whereas only 16 control cases (40%) demonstrated high immunoreactivity (P = 0.007). The CSF-1R antibody showed a positive correlation for local relapse, but no correlation was found between CSF-1R expression and distant metastasis. In summary, our findings provide evidence for the poor prognostic role of CSF-1R in IBTR.     

Tissue culture surface characteristics influence the expansion of human bone marrow cells

Human cell therapy applications in tissue engineering, such as the ex vivo production of hematopoietic cells for transplantation, have recently entered the clinic. Although considerable effort has been focused on the development of biological processes to generate therapeutic cells, little has been published on the design and manufacture of devices for implementation of these processes in a robust and reproducible fashion at a clinical scale. In this study, the effect of tissue culture surface chemistry and texture was assessed in human bone marrow (BM) mononuclear cell (MNC) and CD34-enriched cell cultures. Growth and differentiation was assessed by total, progenitor (CFU-GM), stromal (CFU-F), and primitive (LTC-IC) cell output. Tissue culture treated (TCT) plastic significantly increased MNC culture output as compared with non-TCT plastic, whereas CD34-enriched cell cultures gave lower output (than MNC cultures) that was unaffected by TCT plastic. Interestingly, the level of MNC culture output was significantly different on four commercial TCT surfaces, with the best performing surface giving output that was 1.6- to 2.8-fold greater than the worst one. The surface giving the highest output was the best at supporting development of a distinct morphological feature in the adherent layer (i.e. cobblestone area) indicative of primitive cells, and X-ray photoelectron spectroscopy (XPS) was used to characterize this surface. For custom injection molding of culture devices, the use of three different resins resulted in MNC culture output that was equivalent to commercial cultureware controls, whereas CD34-enriched cell cultures were highly sensitive to resins containing additives. When the texture of molded parts was roughened by sandblasting of the tool, MNC culture output was significantly reduced and higher spikes of IL-6 and G-CSF production were observed, presumably due to macrophage activation. In conclusion, the manufacture of BM MNC culture devices for clinical applications was optimized by consideration of plastic resin, surface treatment, and texture of the culture substratum. Although CD34-enriched cells were insensitive to surface treatment, they were considerably more sensitive to biocompatibility issues related to resin selection. The development of robust systems for BM MNC expansion will enable clinical trials designed to test the safety and efficacy of cells produced in this novel tissue engineering application.     

The regulation of reactive oxygen species production during programmed cell death

Reactive oxygen species (ROS) are thought to be involved in many forms of programmed cell death. The role of ROS in cell death caused by oxidative glutamate toxicity was studied in an immortalized mouse hippocampal cell line (HT22). The causal relationship between ROS production and glutathione (GSH) levels, gene expression, caspase activity, and cytosolic Ca2+ concentration was examined. An initial 5-10-fold increase in ROS after glutamate addition is temporally correlated with GSH depletion. This early increase is followed by an explosive burst of ROS production to 200-400-fold above control values. The source of this burst is the mitochondrial electron transport chain, while only 5-10% of the maximum ROS production is caused by GSH depletion. Macromolecular synthesis inhibitors as well as Ac-YVAD-cmk, an interleukin 1beta-converting enzyme protease inhibitor, block the late burst of ROS production and protect HT22 cells from glutamate toxicity when added early in the death program. Inhibition of intracellular Ca2+ cycling and the influx of extracellular Ca2+ also blocks maximum ROS production and protects the cells. The conclusion is that GSH depletion is not sufficient to cause the maximal mitochondrial ROS production, and that there is an early requirement for protease activation, changes in gene expression, and a late requirement for Ca2+ mobilization.     

Endometriosis and the neoplastic process

Endometriosis is a frequent disorder that commonly presents with infertility and pelvic pain. Although the precise aetiology of endometriosis is unclear, it is generally considered to involve multiple genetic, environmental, immunological, angiogenic and endocrine processes. Genetic factors have been implicated in endometriosis but the susceptibility genes remain largely unknown. Although endometriosis is a benign disorder, recent studies of endometriosis suggest endometriosis could be viewed as a neoplastic process. Evidence to support this hypothesis includes the increased susceptibility to develop ovarian clear-cell and endometrioid cancers in the presence of endometriosis, and molecular similarities between endometriosis and cancer. In this article we discuss (i) the evidence suggesting that endometriosis might be viewed as a neoplastic process, and (ii) the implications of this hypothesis for elucidating the pathogenesis of endometriosis and developing novel methods of diagnostic classification and individualised treatments.     

Sequencing with limited flexibility

We consider the problem of resequencing a set of prearranged jobs when there is limited resequencing flexibility and sequence-dependent changeover costs. Resequencing flexibility is limited by how far forward or backward a job can shift in the sequence relative to its original position. We show how the problem can be solved using dynamic programming in polynomial time with respect to the number of jobs. We also show how the same solution approach can be extended to problems where sequencing constraints are job specific and to problems where job features, which determine changeover costs, are jointly determined with the job sequence. We provide an integer programming formulation to the resequencing problem whose linear programming relaxation offers a useful lower bound. We also describe a family of decomposition heuristics that are easy to customize to provide desired levels of solution quality and solution time. We document the quality of the lower bound from the linear programming relaxation and the upper bound from the heuristic using numerical results. We also provide numerical results to support managerial insights regarding the value of flexibility. We show that the value of flexibility is of the diminishing kind with most of the benefit realized with relatively limited flexibility. We also show that a balanced allocation of flexibility among forward and backward position shifting is superior to an unbalanced one. More significantly, we show that forward and backward position shifting flexibility are complements with the value of one increasing in the amount of the other. Finally, we apply our solution approach to a real-world case from the automotive industry.