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81.
Farzaneh Fayazpour Bart Lucas Roosmarijn E. Vandenbroucke Stefaan Derveaux Jan Tavernier Sam Lievens Joseph Demeester Stefaan C. De Smedt 《Advanced functional materials》2008,18(18):2716-2723
To obtain more biologically relevant data there is a growing interest in the use of living cells for assaying the biological activity of unknown chemical compounds. Density ‘multiplex’ cell‐based assays, where different cell types are mixed in one well and simultaneously investigated upon exposure to a certain compound are beginning to emerge. To be able to identify the cells they should be attached to microscopic carriers that are encoded. This paper investigates how digitally encoded microparticles can be loaded with cells while keeping the digital code in the microcarriers readable. It turns out that coating the surface of the encoded microcarriers with polyelectrolytes using the layer‐by‐layer (LbL) approach provides the microcarriers with a ‘highly functional’ surface. The polyelectrolyte layer allows the growth of the cells, allows the orientation of the cell loaded microcarriers in a magnetic field, and does not hamper the reading of the code. It has further been shown that the cells growing on the polyelectrolyte layer can become transduced by adenoviral particles hosted by the polyelectrolyte layer. It is concluded that the digitally encoded microparticles are promising materials for use in biomedical and pharmaceutical in‐vitro research where cells are used as tools. 相似文献
82.
Ezequiel R. Soulé Guillermo E. Eliçabe Roberto J. J. Williams 《Polymer Bulletin》2008,60(2-3):417-423
An unexpected experimental finding is reported where the primary morphology developed during polymerization-induced
phase separation in a rubber-modified thermoplastic practically disappears at high conversions. This process
was evidenced by light scattering (LS) and scanning electron microscopy (SEM) for a particular composition
of solutions of polyisobutylene oligomers (PIB) in isobornylmethacrylate (IBoMA), during the free-radical
polymerization of the monomer. The primary phase separation produced a dispersion of domains rich in PIB
containing significant amounts of the monomer (IBoMA). Polymerization of the monomer in these domains occurred
at high overall conversions producing the filling of dispersed domains with a PIBoMA-PIB blend that could
not be distinguished from the matrix. Under these conditions the final material had the appearance of a
homogeneous blend. 相似文献
83.
The new emerging area of Enterprise Wide Optimization (EWO) has focused the attention in effectively solving the combined production/distribution scheduling problem. The importance of logistic activities performed in multi-site environments comes from the relative magnitude of the associated transportation costs and the good chance of getting large savings on such expenses. This paper first develops an exact MILP mathematical formulation for the multiple vehicle time-window-constrained pickup and delivery (MVPDPTW) problem. The approach is able to account for many-to-many transportation requests, pure pickup and delivery tasks, heterogeneous vehicles and multiple depots. Optimal solutions for a variety of benchmark problems with cluster/random distributions of pickup and delivery locations and limited sizes in terms of customer requests and vehicles have been discovered. However, the computational cost exponentially grows with the number of requests. For large-scale m-PDPTW problems, a local search improvement algorithm steadily providing a better solution through two evolutionary steps is also presented. A neighborhood structure around the starting solution is generated by first allowing multiple request exchanges among nearby trips and then permitting the reordering of nodes on every individual route. If a better set of routes is found, both steps are repeated until no improved solution is discovered. Compact MILP mathematical formulations for both sub-problems have been developed and solved through an efficient branch-and-bound algorithm. A significant number of large-scale m-PDPTW benchmark problems, some of them including up to 100 transportation requests, were successfully solved in reasonable CPU times. 相似文献
84.
85.
Vanesa Rodríguez-Amor Juan P. Fernández-Blázquez Antonio Bello Ernesto Pérez María L. Cerrada 《Polymer Bulletin》2008,60(1):89-96
Summary The effect of molecular weight on the uniaxial orientation process has been analyzed in two samples of thermotropic poly(diethylene
glycol p,p′-bibenzoate), evaluating the influence of that parameter on the type of orientation obtained. Several strain rates and deformation
temperatures have been tested in order to map out the conditions for obtaining the two different kinds of orientation. The
results show that in the lower molecular weight PDEB sample (Mw=31200) it is rather easy to get exclusively perpendicular “anomalous” orientation, with the molecular axes aligned transversely
to the stretching direction. However, it is extremely difficult to obtain 100% parallel “normal” orientation. On the contrary,
either type of orientation or a mixture of them can be easily developed for the higher molecular weight PDEB sample (Mw=102900). 相似文献
86.
87.
Mirna Suárez-Quiroz Béatrice De Louise Oscar Gonzalez-Rios Michel Barel Bernard Guyot Sabine Schorr-Galindo & Joseph-Pierre Guiraud 《International Journal of Food Science & Technology》2005,40(6):605-611
Roasting coffee led to a drop in the ochratoxin A (OTA) concentration, as measured by the reference method, especially for dark type roasts. The way the beverage was prepared also affected the OTA content, which could paradoxically be higher than that of the initial roasted coffee. Assays on the thermal stability of pure OTA showed that it ought to be found in larger quantities in roasted coffee. This suggested that OTA was masked by reactions with the substrate during roasting. The absence of OTA in green coffee is therefore the best guarantee of safety. 相似文献
88.
Sebastián López Gustavo M. Callicó Félix Tobajas Valentín de Armas José F. López Roberto Sarmiento 《ETRI Journal》2008,30(6):862-864
This letter presents a novel approach for organizing computational resources into groups within H.264/AVC motion estimation architectures, leading to reductions of up to 75% in the equivalent gate count with respect to state‐of‐the‐art designs. 相似文献
89.
M Isabel Sánchez de Rojas Julián Rivera Moisés Frías Félix Marín 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2008,83(3):209-217
Copper slag is a by‐product generated during smelting to extract copper metal from the ore. The copper slag obtained may exhibit pozzolanic activity and may therefore be used in the manufacture of addition‐containing cements. In this paper the effect of the incorporation of the copper slag in cement is measured. Blends of copper slag with Portland cement generally possess properties equivalent to Portland cement containing fly ash, but very different to the silica fume incorporation. Copper slag and fly ash reduce the heat of hydration more effectively than silica fume in mortars. The replacement of 30% cement by copper slag reduces the flexural and compressive strength in a similar way to fly ash; however, after 28 days, the reduction is less than the percentage of substitution. Hydrated calcium aluminate phases were analysed using scanning electron microscopy (SEM) and X‐ray diffraction (XRD) techniques. The pozzolanic activity of copper slag is similar to that of fly ash and higher than silica fume. In the presence of low water/cement ratios, certain pozzolanic materials produce a very compact cement paste that limits the space available for hydration products, a determining factor in the formation of hydrated calcium aluminates. SEM was found to be a useful analytical technique when aluminates are formed and can be clearly detected by XRD. Copyright © 2008 Society of Chemical Industry 相似文献
90.
Joaquín Cortés Eliana Valencia Gonzalo Aguila Esteban Orellana Paulo Araya 《Catalysis Letters》2008,126(1-2):63-71
An experimental study is made of the time decay of activity of the CO–NO reaction on a Pd/Al2O3 looking at the effect on reaction order and apparent activation energy. The optimum kinetics parameters fitting the steady state data at moderate pressures are determined. The time decay curves are analyzed through various catalyst deactivation models. 相似文献