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31.
Coton M Delbés-Paus C Irlinger F Desmasures N Le Fleche A Stahl V Montel MC Coton E 《Food microbiology》2012,29(1):88-98
The goal of this study was to identify at the species level a large collection of Gram-negative dairy bacteria isolated from milks or semi-hard and soft, smear-ripened cheeses (cheese core or surface samples) from different regions of France. The isolates were then assessed for two risk factors, antibiotic resistance and volatile and non-volatile biogenic amine production in vitro. In total, 173 Gram-negative isolates were identified by rrs and/or rpoB gene sequencing. A large biodiversity was observed with nearly half of all Gram-negative isolates belonging to the Enterobacteriaceae family. Overall, 26 different genera represented by 68 species including potential new species were identified among the studied Gram-negative isolates for both surface and milk or cheese core samples. The most frequently isolated genera corresponded to Pseudomonas, Proteus, Psychrobacter, Halomonas and Serratia and represented almost 54% of the dairy collection. After Pseudomonas, Chryseobacterium, Enterobacter and Stenotrophomonas were the most frequently isolated genera found in cheese core and milk samples while Proteus, Psychrobacter, Halomonas and Serratia were the most frequently isolated genera among surface samples. Antibiotic resistance profiles indicated that resistances to the aminosid, imipemen and quinolon were relatively low while more than half of all tested isolates were resistant to antibiotics belonging to the monobactam, cephem, fosfomycin, colistin, phenicol, sulfamid and some from the penam families. Thirty-six% of isolates were negative for in vitro biogenic amine production. Among biogenic amine-producers, cadaverine was the most frequently produced followed by isoamylamine, histamine and putrescine. Only low levels (<75 mg/l) of tyramine were detected in vitro. 相似文献
32.
Willy Charon Marie-Christine Iltchev Jean-François Blachot 《International Journal of Hydrogen Energy》2014
The control of the performance of a fuel cell needs the knowledge of mechanical stresses. A finite element model submitted to operational static loads is developed for pure mechanical analysis of a stack. Adapted to practical implementation in the frame of industrial projects, the tool runs on work stations. Modelling and simulations at the early stage of the stack architecture development are mandatory to lower the costs of the stack and to contribute in designing component dimensions and forms, particularly of stamped metallic bipolar plates or clamping systems. The model should also be useful for accurate local analyses and channel geometry of metallic bipolar plates must be modelled in detail. But the number of degrees of freedom is huge and some model simplification is required to compute a whole stack. The homogenisation technique was applied, replacing cell parts with composite finite elements or homogenised representative elementary volumes. 相似文献
33.
J. Rannou A. Gravouil M. C. Baïetto‐Dubourg 《International journal for numerical methods in engineering》2009,77(4):581-600
A multiscale method for 3‐D crack propagation simulation in large structures is proposed. The method is based on the extended finite element method (X‐FEM). The asymptotic behavior of the crack front is accurately modeled using enriched elements and no remeshing is required during crack propagation. However, the different scales involved in fracture mechanics problems can differ by several orders of magnitude and industrial meshes are usually not designed to account for small cracks. Enrichments are therefore useless if the crack is too small compared with the element size. To overcome this drawback, a project combining different numerical techniques was started. The first step was the implementation of a global multigrid algorithm within the X‐FEM framework and was presented in a previous paper (Eur. J. Comput. Mech. 2007; 16 :161–182). This work emphasized the high efficiency in cpu time but highlighted that mesh refinement is required on localized areas only (cracks, inclusions, steep gradient zones). This paper aims at linking the different scales by using a local multigrid approach. The coupling of this technique with the X‐FEM is described and computational aspects dealing with intergrid operators, optimal multiscale enrichment strategy and level sets are pointed out. Examples illustrating the accuracy and efficiency of the method are given. Copyright © 2008 John Wiley & Sons, Ltd. 相似文献
34.
Marie-Christine Lebart Franoise Trousse Gilles Valette Joan Torrent Morgane Denus Nadine Mestre-Frances Anne Marcilhac 《International journal of molecular sciences》2022,23(15)
Reg-1α/lithostathine, a protein mainly associated with the digestive system, was previously shown to be overexpressed in the pre-clinical stages of Alzheimer’s disease. In vitro, the glycosylated protein was reported to form fibrils at physiological pH following the proteolytic action of trypsin. However, the nature of the protease able to act in the central nervous system is unknown. In the present study, we showed that Reg-1α can be cleaved in vitro by calpain-2, the calcium activated neutral protease, overexpressed in neurodegenerative diseases. Using chemical crosslinking experiments, we found that the two proteins can interact with each other. Identification of the cleavage site using mass spectrometry, between Gln4 and Thr5, was found in agreement with the in silico prediction of the calpain cleavage site, in a position different from the one reported for trypsin, i.e., Arg11-Ile12 peptide bond. We showed that the cleavage was impeded by the presence of the neighboring glycosylation of Thr5. Moreover, in vitro studies using electron microscopy showed that calpain-cleaved protein does not form fibrils as observed after trypsin cleavage. Collectively, our results show that calpain-2 cleaves Reg-1α in vitro, and that this action is not associated with fibril formation. 相似文献
35.
G Jondeau O Dubourg G Delorme JF Arnal F Chikli L Kamoun C Dumas JP Bourdarias 《Canadian Metallurgical Quarterly》1994,15(2):242-246
We performed a double-blind, placebo-controlled study to determine whether oral enoximone would aid weaning dobutamine-dependent patients. Twenty-four patients 64 +/- 10 years, with an echocardiographic ejection fraction of 0.20 +/- 0.06, and receiving maximal therapy were studied. After failure of dobutamine weaning, a dobutamine infusion was set up at 10 micrograms.kg-1.min-1 for 48 h. Oral enoximone (100 mg t.i.d.) or placebo was added from D0 for the next 28 days, while the dobutamine dosage was progressively decreased after D4 and eventually stopped at D7. The patients were then followed-up for 21 days (i.e. until enoximone administration had continued for 28 days). In the placebo group, two patients suffered a relapse of congestive heart failure (CHF) before D4, six patients withdrew during dobutamine tapering (five with a relapse of CHF and one with septic shock) and two during follow-up (one with a relapse of CHF and one with sustained ventricular tachycardia). In the enoximone group, three patients withdrew during dobutamine tapering (two with a relapse of CHF, one with a cutaneous rash). Four patients on placebo and nine receiving enoximone could be weaned from dobutamine, P < 0.05. Echocardiographic LV ejection fraction significantly increased and Doppler-derived indexes of systolic function tended to increase when enoximone but not placebo was associated with dobutamine. Oral enoximone might be helpful in weaning patients with end-stage congestive heart failure from i.v. dobutamine. 相似文献
36.
A theoretical model for the prediction of initial growth angles and sites of fretting fatigue cracks
The crack initiation mechanisms under fretting fatigue conditions are investigated. Fretting fatigue tests have been conducted on an aluminium alloy. Two major crack initiation mechanisms are experimentally observed. They are theoretically identified by employing a simple dislocation dipole model and taking into consideration the reverse sliding along dislocation layers and the influence of the distance between these layers. It is shown that cracks may initiate either by an extrusion-intrusion mechanism or a fatigue tensile process. Two parameters respectively linked to each of these initiation mechanisms are proposed to predict crack location. The theoretical crack initial growth directions and locations are shown to correlate very well with the experimental ones. With regards to these initiation processes, a spall detachment mechanism is proposed considering the interaction of microcracks. 相似文献
37.
Marie-Christine?Daniel Jaime?Ruiz?Aranzaes Sylvain?Nlate Didier?AstrucEmail author 《Journal of Inorganic and Organometallic Polymers and Materials》2005,15(1):107-119
Polyamidoferrocenylalkylthiolate-Au nanoparticles (AuNP) and AuNP-cored polyferrocenyl dendrimers have been assembled either by the ligand-substitution method from dodecanethiolate-AuNP (AB3 units) or Brust-type direct synthesis from a 1-1 mixture of dodecanethiol and dendronized thiol (AB9 units). The AuNP-cored polyferrocenyl dendrimers are a new type of dendrimers with a AuNP core. AuNPs containing a nonasilylferrocenyl dendron have been synthesized; they bear respectively 180 and 360 ferrocenyl units at the periphery. These AuNPs selectively recognize the anions H2PO4– and adenosine-5-triphosphate (ATP2–) with a positive dendritic effect. Recognition is monitored by the appearance of a new wave at a less positive potential in cyclic voltammetry (CV). These AuNP-cored polyferrocenyl dendrimers can form robust modified electrodes that serve as re-usable sensors of ATP2– by simple washing with CH2Cl2. 相似文献
38.
39.
Benjamin Negrevergne Alexandre Termier Marie-Christine Rousset Jean-François Méhaut 《Data mining and knowledge discovery》2014,28(3):593-633
In this paper, we present Para Miner which is a generic and parallel algorithm for closed pattern mining. Para Miner is built on the principles of pattern enumeration in strongly accessible set systems. Its efficiency is due to a novel dataset reduction technique (that we call EL-reduction), combined with novel technique for performing dataset reduction in a parallel execution on a multi-core architecture. We illustrate Para Miner’s genericity by using this algorithm to solve three different pattern mining problems: the frequent itemset mining problem, the mining frequent connected relational graphs problem and the mining gradual itemsets problem. In this paper, we prove the soundness and the completeness of Para Miner. Furthermore, our experiments show that despite being a generic algorithm, Para Miner can compete with specialized state of the art algorithms designed for the pattern mining problems mentioned above. Besides, for the particular problem of gradual itemset mining, Para Miner outperforms the state of the art algorithm by two orders of magnitude. 相似文献
40.
Hilal Balout Pascal Boulet Marie-Christine Record 《Journal of Electronic Materials》2013,42(12):3458-3466
The electronic and thermoelectric properties of biaxially strained magnesium silicide Mg2Si are analyzed by means of first-principle calculations and semiclassical Boltzmann theory. Electron and hole doping are examined for different doping concentrations and temperatures. Under strain the degeneracy of the electronic orbitals near the band edges is removed, the orbital bands are warped, and the energy gap closes up. These characteristics are rationalized in the light of the electron density transfers upon strain. The electrical conductivity increases with the biaxial strain, whereas neither the Seebeck coefficient nor the power factor (PF) follow this trend. Detailed analysis of the evolution of these thermoelectric properties is given in terms of the in-plane and cross-plane components. Interestingly, the maximum value of the PF is shifted towards lower temperatures when increasingly intensive strain is applied. 相似文献