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101.
M Kawase M Fujimura Y Morita-Fujimura PH Chan 《Canadian Metallurgical Quarterly》1999,30(2):441-8; discussion 449
BACKGROUND AND PURPOSE: To clarify the relationship between apurinic/apyrimidinic endonuclease (APE/Ref-1), a multifunctional protein in the DNA base excision repair pathway, and delayed neuronal cell death associated with apoptosis, we examined the expression of APE/Ref-1 before and after transient global ischemia in rats. METHODS: Global ischemia was induced by bilateral common carotid artery occlusion and hypotension. Expression of the APE/Ref-1 protein was evaluated by Western blot and immunohistochemical analyses. Apoptosis after global ischemia was observed by DNA electrophoresis and terminal deoxynucleotidyl transferase-mediated uridine 5'-triphosphate-biotin nick end labeling (TUNEL) staining. RESULTS: Immunohistochemistry showed the nuclear expression of APE/Ref-1 in the control brains. Nuclear immunoreactivity of APE/Ref-1 was significantly decreased 2 days after 10 minutes of ischemia in the hippocampal CA1 subregion. Western blot analysis of a sample from the normal brains showed a characteristic 37-kDa band, which was reduced in the hippocampal CA1 subregion after ischemia. A significant amount of DNA fragmentation was observed at 3 days but not at 1 day after ischemia. Double staining with APE/Ref-1 and TUNEL clearly showed that the neurons that lost APE/Ref-1 immunoreactivity became TUNEL positive. CONCLUSIONS: Our data provide evidence that APE/Ref-1 decreased in hippocampal CA1 neurons after transient global ischemia and that this reduction precedes DNA fragmentation, which is destined to cause apoptosis. Our results suggest the possibility that a decrease of APE/Ref-1 activity and the failure of DNA repair may underlie the mechanism of apoptosis after transient focal ischemia. 相似文献
102.
This paper clarifies the mechanism of how electromagnetic force applied to the wire electrode in wire electrical discharge machining (wire-EDM) is generated. This electromagnetic force is caused not only by DC component but also by AC components of the discharge current supplied to the wire. We therefore developed and used a two-dimensional finite element method (FEM) program to analyze the electromagnetic field taking into account electromagnetic induction. Assuming that trapezoidal pulse current is supplied to the wire, distributions of the current density and magnetic flux density were analyzed and changes in the electromagnetic force applied to the wire were calculated. Wire movement when the electromagnetic force alone was applied to the wire was also calculated. The calculated wire movement agreed with the measured wire movement when pulse current actually used in WEDM was supplied to the wire, clarifying the mechanism of electromagnetic force generation. 相似文献
103.
K Sakamoto M Arakawa M Ohmori K Sugimoto A Fujimura 《Canadian Metallurgical Quarterly》1997,61(13):1253-1259
The daily variation in an intensity of kaolin-induced writhing reaction was examined in mice kept under conditions of light; 07:00 - 19:00 and dark; 19:00 - 07:00. The number of writhes was counted for 30 minutes after a single intraperitoneal injection of kaolin at 00:00, 02:00, 04:00, 06:00, 08:00, 10:00, 12:00, 14:00, 16:00, 18:00, 20:00 and 22:00. The number of writhes showed a daily variation with a peak at 18:00 and a trough at 06:00. The intensity of writhing reaction was significantly reduced by pretreatment with the bradykinin B1 (Des-Arg9-[Leu8]-BK) and B2 (icatibant) receptor antagonists. Significant daily variation in this parameter was still observed in the group with the B1 antagonist, but disappeared in the B2 antagonist-treated group. These results suggest that the kaolin-induced writhing reaction shows the daily variation with a peak at the end of the resting period and a trough at the end of the active period. The B2 receptor mediated stimuli appears to be involved in this phenomenon. 相似文献
104.
Anatase-type TiO2 solid solutions doped with 0–10 mol% scandium were formed by hydrothermal crystallization under weak basic conditions above 180°C for 5 h from amorphous co-precipitates that were obtained from the aqueous precursor solutions of TiOSO4 and Sc(NO3 )3 using aqueous ammonia. The anatase particles were spindle-like and consisted of nanosized-crystallites (23–25 nm). The lattice parameter c 0 of anatase and the length and width of the spindle-like anatase gradually increased when the scandium content was increased. The diffuse reflectance spectra of the as-prepared TiO2 doped with scandium showed that the onset of absorption slightly shifted to longer wavelengths with increasing scandium content. The band gap of anatase was slightly increased by making solid solutions with scandium oxide. 相似文献
105.
Takeshi Kikuchi Makio Mogi Iichiro Okabe Kosuke Okada Hisashi Goto Yasuyuki Sasaki Takeki Fujimura Mitsuo Fukuda Akio Mitani 《International journal of molecular sciences》2015,16(10):24111-24126
Periodontal disease is caused by dental plaque biofilms, and the removal of these biofilms from the root surface of teeth plays a central part in its treatment. The conventional treatment for periodontal disease fails to remove periodontal infection in a subset of cases, such as those with complicated root morphology. Adjunctive antimicrobial photodynamic therapy (aPDT) has been proposed as an additional treatment for this infectious disease. Many periodontal pathogenic bacteria are susceptible to low-power lasers in the presence of dyes, such as methylene blue, toluidine blue O, malachite green, and indocyanine green. aPDT uses these light-activated photosensitizer that is incorporated selectively by bacteria and absorbs a low-power laser/light with an appropriate wavelength to induce singlet oxygen and free radicals, which are toxic to bacteria. While this technique has been evaluated by many clinical studies, some systematic reviews and meta-analyses have reported controversial results about the benefits of aPDT for periodontal treatment. In the light of these previous reports, the aim of this review is to provide comprehensive information about aPDT and help extend knowledge of advanced laser therapy. 相似文献
106.
Ikutaro Okada Koji Takahashi Wenqiang Zhang Xiaofu Zhang Hongyu Yang Shigeru Fujimura 《IEEJ Transactions on Electrical and Electronic Engineering》2014,9(2):190-199
In this paper, we aim to solve the problem of resource‐constrained project scheduling with multiple modes (rc‐PSP/mM), in which multiple execution modes are available for each of the project's activity and with minimization of makespan as objective. We present a new genetic algorithm approach in order to solve this problem. In this procedure, we propose a new mutation operator that exploits a critical path and two new local search procedures, i.e. critical path improvement local search (cpiLS) and iterative forward/backward local search (ifbLS), using activity list characteristics. The cpiLS reduces the critical path and the ifbLS improves resource allocation of the schedule of rc‐PSP/mM. Also, to evaluate the proposed approach, we compare the results of the computational experiment on certain standard project instances with the several competing heuristic procedures presented in the literature, and it has been revealed that our procedure is one of the most competitive among the algorithms. © 2014 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc. 相似文献
107.
These days the number of projects trying to urge a change in the occupant's behaviour towards a sustainable one is increasing. However, still less is known about the effect of such measures. This paper describes the findings of two investigations, a field measurement and an Internet-based survey, both including the dissemination of information about strategies for a high level of comfort without much energy usage. The focus was on the ability to quantify the effect of such measures on the heating and cooling behaviour. As a result, those who participated in a workshop were more likely to change their behaviour than those who received an information brochure only; whether this was due to the method employed or the type of participants could not be ascertained. However, the workshop participants reduced their cooling device usage by up to 16%. The concept of exergy was used to show how this reduction affects the exergy consumption of the cooling device, because it enables us to consider the qualitative aspect of energy as a quantity to be calculated. This showed that the exergy consumed by the workshop group was reduced by up to 20% comparing their behaviour before and after the information dissemination. 相似文献
108.
Tomonari M Ida K Yamashita H Yonezawa T 《Journal of nanoscience and nanotechnology》2008,8(5):2468-2471
Size-controlled oxidation-resistant copper fine particles were prepared from insoluble CuO micron-sized particles. The particle sizes were quite uniform and could be varied only by the concentration of the complex reagent from 45 to 175 nm. No template material was needed for size control. Gelatin was selected as the protective polymer. Addition of protease after formation of copper fine particles decomposed preferentially loop and tail parts of gelatin. The remaining nanoskin gelatin layer, covered on the particle surface, prevents oxidation of copper. 相似文献
109.
The activities of FexO in CaO + CaF2 + SiO2 + FexO quaternary slags were measured by means of solid-oxide galvanic cell. The FexO activities in the slags are influenced by CaF2 as well as SiO2. At constant FexO mole fractions, e.g., , at low SiO2 mole fractions i.e., , the substitution of CaF2 for CaO has an effect of raising the FexO activity. At higher SiO2 mole fractions, e.g., , however, such an effect becomes insignificant. 相似文献
110.
Masanori Suzuki Norimasa Umesaki Yoshiki Ishii 《Journal of the American Ceramic Society》2022,105(1):700-711
Dicalcium silicate, which is found in steelmaking slag for dephosphorization, exists as the hexagonal α phase at high temperatures. The α-dicalcium silicate forms a solid solution with tricalcium phosphate in the entire composition range, although the reason for high solubility of phosphorus remains unclear in view of the crystal structure. It has previously been reported that the crystal structure of α-dicalcium silicate consists of a symmetric arrangement of Ca2+ ions and SiO44− tetrahedra, although other polymorphs exhibit asymmetric arrangements. However, because the occupation probability of each atomic site in the α polymorph is not limited to unity, it has not been qualified how these ions are exactly arranged. In this study, the ionic distribution in the α polymorph of dicalcium silicate was evaluated by first-principles calculation based on density functional theory (DFT), as well as by molecular dynamics (MD) simulation with a polarizable ion model optimized by DFT calculation. The results indicated that the completely symmetric ionic arrangement, as reported for the α phase, is the most unstable. Electronic-state calculation and MD simulation indicated that a highly disordered ionic arrangement spontaneously forms in the α-phase crystal for structure relaxation when held at high temperatures, or when phosphorus is incorporated. 相似文献