Charged Particle Trajectory Statistics and Deposition in a Turbulent Channel Flow

The statistical properties of charged particles and their wall deposition in a turbulent channel flow in the presence of an electrostatic field is studied in this paper. For a dilute concentration, the influence of small particles on the fluid motion is neglected. The instantaneous velocity field is generated by a direct numerical simulation of the Navier-Stokes equation via a pseudospectral method. The case in which each particle carries a single unit of charge and the case in which the particles have a saturation charge distribution are analyzed. Ensembles of 8192 particle trajectories are used for evaluating various statistics. Effects of size and electric field intensity on particle trajectory statistics and wall deposition rate are studied. RMS particle velocities and particle concentrations at different distances from the wall are evaluated and discussed. The results for deposition rates are compared with those obtained from empirical equations.     

Efficient PCR-based gene disruption in Saccharomyces strains using intergenic primers

Gene disruptions are a vital tool for understanding Saccharomyces cerevisiae gene function. An arrayed library of gene disruption strains has been produced by a consortium of yeast laboratories; however their use is limited to a single genetic background. Since the yeast research community works with several different strain backgrounds, disruption libraries in other common laboratory strains are desirable. We have developed simple PCR-based methods that allow transfer of gene disruptions from the S288C-derived strain library into any Saccharomyces strain. One method transfers the unique sequence tags that flank each of the disrupted genes and replaces the kanamycin resistance marker with a recyclable URA3 gene from Kluyveromyces lactis. All gene-specific PCR amplifications for this method are performed using a pre-existing set of primers that are commercially available. We have also extended this PCR technique to develop a second general gene disruption method suitable for any transformable strain of Saccharomyces.     

Osmotic and activity coefficients in the solutions of 1- and 2-naphthol in methanol and ethanol at 298.15 K

The osmotic coefficient of the solution, activity of the solvent and activity coefficient of the solute in solutions of 1- and 2-naphthol in methanol and ethanol have been measured by the isopiestic method. The experimental osmotic coefficient data were correlated using the Non-Random Two Liquid model (NRTL), the Non-Random Factor model (NRF), the two suffix Margules equation and a polynomial in terms of the molality of the solute. The models give reliable results for the correlation of the osmotic coefficient data. The activity coefficients of 1- and 2-naphthol were evaluated according to the considered methods using the parameters obtained by the correlation of osmotic coefficients.     

Utilizing Voronoi Cells of Location Data Streams for Accurate Computation of Aggregate Functions in Sensor Networks

Sensor networks are unattended deeply distributed systems whose database schema can be conceptualized using the relational model. Aggregation queries on the data sampled at each sensor node are the main means to extract the abstract characteristics of the surrounding environment. However, the non-uniform distribution of the sensor nodes in the environment leads to inaccurate results generated by the aggregation queries. In this paper, we introduce “spatial aggregations” that take into consideration the spatial location of each measurement generated by the sensor nodes. We propose the use of spatial interpolation methods derived from the fields of spatial statistics and computational geometry to answer spatial aggregations. In particular, we study Spatial Moving Average (SMA), Voronoi Diagram and Triangulated Irregular Network (TIN). Investigating these methods for answering spatial average queries, we show that the average value on the data samples weighted by the area of the Voronoi cell of the corresponding sensor node, provides the best precision. Consequently, we introduce an algorithms to compute and maintain the accurate Voronoi cell at each sensor node while the location of the others arrive on data stream. We also propose AVC-SW, a novel algorithm to approximate this Voronoi cell over a sliding window that supports dynamism in the sensor network. To demonstrate the performance of in-network implementation of our aggregation operators, we have developed prototypes of two different approaches to distributed spatial aggregate processing. Permission to make digital or hard copies of all or part of this work for personal or classroom use is granted without fee provided that copies are not made or distributed for profit or commercial advantage and that copies bear this notice and the full citation on the first page. To copy otherwise, to republish, to post on servers or to redistribute to lists, requires prior specific permission and/or a fee. GIS'04, November 12–13, 2004, Washington DC, USA. Copyright 2004 ACM 1-58113-979-9/04/0011...$5.00.     

The design process of expert systems development: some concerns

Abstract: A discussion is presented of why some expert systems that organizations have developed are not successful. The concept of design process plays a very significant role at the outset of the expert system development process. This concept has not been the subject of much debate and attention in expert systems development. From the author's point of view, one of the main issues is how the designer (knowledge engineer) thinks about the design process. In general, the designer's process is influenced by the knowledge engineer's conception. This paper endeavors to disclose some of the main factors related to the knowledge engineer's conception of the design process and an attempt is made to put forward a conceptual model of the expert system design process. This conceptual model is an initial step towards a successful implementation of expert system projects.     

Measurements on a 215-GHz subharmonically pumped waveguide mixerusing planar back-to-back air-bridge Schottky diodes

This paper presents design and performance data for a 215-GHz subharmonically pumped waveguide mixer using an antiparallel-pair of planar air-bridge-type GaAs Schottky-barrier diodes. The waveguide design is a prototype for a 640-GHz system and uses split-block rectangular waveguide with a 2:1 width-to-height ratio throughout. The measured mixer noise and conversion loss are below that of the best reported whisker contacted or planar-diode mixers using the subharmonic-pump configuration at this frequency. In addition, the required local oscillator power is as low as 3 mW for the unbiased diode pair, and greater than 34 dB of LO noise suppression is observed. Separate sideband calibration, using a Fabry-Perot filter, indicates that the mixer can be tuned for true double sideband response at an intermediate frequency of 1.5 GHz. Microwave scale model measurements of the waveguide mount impedances are combined with a mixer nonlinear analysis computer program to predict the mixer performance as a function of anode diameter, anode finger inductance, and pad-to-pad fringing capacitance. The computed results are in qualitative agreement with measurements, and indicate that careful optimization of all three diode parameters is necessary to significantly improve the mixer performance     

Analysis of underivatized amino acids and their D/L-enantiomers by sheathless capillary electrophoresis/electrospray ionization mass spectrometry

Capillary electrophoresis/electrospray ionization-mass spectrometry (CE/ESI-MS) was applied to the analysis of underivatized amino acids and the separation of their D/L-enantiomers. Under full-scan mode, all standard protein amino acids were separated and detected at low-femtomole levels using a 130-cm-long, 20-microm-i.d., 150-microm-o.d. underivatized fused-silica capillary with 1 M formic acid as the background electrolyte. The CE/ESI-MS technique was also applied to the separation of L-arginine from L-canavanine (a close analogue of arginine where the terminal methylene linked to the guanidine group of arginine is replaced by an oxygen atom) in a complex mixture containing all standard protein amino acids. The utility of CE/ESI-MS in the analysis of real-world samples was demonstrated by the identification of two metabolic diseases (PKU and tyrosinemia) through blood analysis with minimal sample preparation. In addition, the on-line separation of 11 underivatized L-amino acids from their D-enantiomers was achieved by using a 30 mM solution of (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid as the background electrolyte.     

CE/electrospray ionization-MS analysis of underivatized d/l-amino acids and several small neurotransmitters at attomole levels through the use of 18-crown-6-tetracarboxylic acid as a complexation reagent/background electrolyte

A new capillary electrophoresis/mass spectrometry technique is introduced for attomole detection of primary amines (including several neurotransmitters), amino acids, and their d/l enantiomers in one run through the use of a complexation reagent while using only approximately 1 nL of sample. The technique uses underivatized amino acids in conjunction with an underivatized capillary, which significantly reduces cost and analysis time. It was found that when (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid (18-C-6-TCA, MW 440) was used as the background electrolyte/complexation reagent during the capillary electrophoresis/electrospray ionization-mass spectrometry (CE/ESI-MS) analysis of underivatized amino acids, stable complexes were formed between the amino acids and the 18-C-6-TCA molecules. These complexes, which exhibited high ionization efficiencies, were detectable at attomole levels for most amino acids. The detection limits of the AA/18-C-6-TCA complexes were on the average more than 2 orders of magnitude lower than that of the free amino acids in solution. In addition to lower detection limits under CE/ESI-MS, a solution of 18-C-6-TCA in the concentration range of 5-30 mM provided high separation efficiency for mixtures of l-amino acids as well as mixtures of d/l-amino acids. By using a solution of 18-C-6-TCA as the background electrolyte in conjunction with an underivatized, 130-cm-long, 20-microm-i.d., 150-microm-o.d. fused-silica capillary and by monitoring the m/z range of the amino acid/18-C-6-TCA complexes (m/z 515-700), most of the standard amino acids and many of their enantiomers were separated and detected with high separation efficiency and high sensitivity (nanomolar concentration detection limits) in one run. The solutions of 18-C-6-TCA also worked well as the CE/ESI-MS BGE for low-level detection of several neurotransmitters and some of their d/l enantiomers as well as for the analysis of amino acids at endogenous levels in lysed red blood cells.     

Identifying a control function in parabolic partial differential equations from overspecified boundary data

Determination of an unknown time-dependent function in parabolic partial differential equations, plays a very important role in many branches of science and engineering. In the current investigation, the Adomian decomposition method is used for finding a control parameter p(t) in the quasilinear parabolic equation u_t=u_xx+p(t)u+, in [0,1]×(0,T] with known initial and boundary conditions and subject to an additional condition in the form of which is called the boundary integral overspecification. The main approach is to change this inverse problem to a direct problem and then solve the resulting equation using the well known Adomian decomposition method. The decomposition procedure of Adomian provides the solution in a rapidly convergent series where the series may lead to the solution in a closed form. Furthermore due to the rapid convergence of Adomian’s method, a truncation of the series solution with sufficiently large number of implemented components can be considered as an accurate approximation of the exact solution. This method provides a reliable algorithm that requires less work if compared with the traditional techniques. Some illustrative examples are presented to show the efficiency of the presented method.