Characteristics of evolution of mining-induced stress field in the longwall panel:insights from physical modeling

The evolution of mining-induced stress field in longwall panel is closely related to the fracture field and the breaking characteristics of strata.Few laborator...     

Influence of residual chirality on the conformation and enzymatic degradation of glycopolypeptide based biomaterials

Science China Technological Sciences - Glycopolypeptides as analogs of glycoproteins or glycosaminoglycans represent attractive building blocks for the construction of biomimetic biomaterials....     

The Anticancer Activity Conferred by the Mud Crab Antimicrobial Peptide Scyreprocin through Apoptosis and Membrane Disruption

Scyreprocin is an antimicrobial peptide first identified in the mud crab Scylla paramamosain. Herein, we showed that its recombinant product (rScyreprocin) could significantly inhibit the growth of human lung cancer NCI-H460 cells (H460), but showed no cytotoxicity to human lung fibroblasts (HFL1). rScyreprocin was a membrane-active peptide that firstly induced the generation of reactive oxygen species (ROS) in H460, and led to endoplasmic reticulum stress and Ca²⁺ release, which resulted in mitochondrial dysfunction and subsequently activation of caspase-3 cascades, and ultimately led to apoptosis. The comprehensive results indicated that rScyreprocin exerted anticancer activity by disrupting cell membrane and inducing apoptosis. The in vivo efficacy test demonstrated that intratumoral injection of rScyreprocin significantly inhibited the growth of H460 xenografts, which was close to that of the cisplatin (inhibition rate: 69.94% vs. 80.76%). Therefore, rScyreprocin is expected to become a promising candidate for the treatment of lung cancer.     

Genome-Wide Association Study Identifies a Rice Panicle Blast Resistance Gene,Pb2, Encoding NLR Protein

Rice blast is one of the main diseases in rice and can occur in different rice growth stages. Due to the complicated procedure of panicle blast identification and instability of panicle blast infection influenced by the environment, most cloned rice resistance genes are associated with leaf blast. In this study, a rice panicle blast resistance gene, Pb2, was identified by genome-wide association mapping based on the panicle blast resistance phenotypes of 230 Rice Diversity Panel 1 (RDP1) accessions with 700,000 single-nucleotide polymorphism (SNP) markers. A genome-wide association study identified 18 panicle blast resistance loci (PBRL) within two years, including 9 reported loci and 2 repeated loci (PBRL2 and PBRL13, PBRL10 and PBRL18). Among them, the repeated locus (PBRL10 and PBRL18) was located in chromosome 11. By haplotype and expression analysis, one of the Nucleotide-binding domain and Leucine-rich Repeat (NLR) Pb2 genes was highly conserved in multiple resistant rice cultivars, and its expression was significantly upregulated after rice blast infection. Pb2 encodes a typical NBS-LRR protein with NB-ARC domain and LRR domain. Compared with wild type plants, the transgenic rice of Pb2 showed enhanced resistance to panicle and leaf blast with reduced lesion number. Subcellular localization of Pb2 showed that it is located on plasma membrane, and GUS tissue-staining observation found that Pb2 is highly expressed in grains, leaf tips and stem nodes. The Pb2 transgenic plants showed no difference in agronomic traits with wild type plants. It indicated that Pb2 could be useful for breeding of rice blast resistance.     

Green revolution in electronic displays expected to ease energy and health crises

The technological revolution of long-awaited energy-saving and vision-friendly displays represented by bistable display technology is conning.Here we discuss methods,challenges,and opportunities for implementing bistable displays in terms of molecular design,device structure,further expansion,and required criteria,hopefully benefiting the light-related community.     

Trans-Reduction of Cerebral Small Vessel Disease Proteins by Notch-Derived EGF-like Sequences

Cysteine oxidation states of extracellular proteins participate in functional regulation and in disease pathophysiology. In the most common inherited dementia, cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), mutations in NOTCH3 that alter extracellular cysteine number have implicated NOTCH3 cysteine states as potential triggers of cerebral vascular smooth muscle cytopathology. In this report, we describe a novel property of the second EGF-like domain of NOTCH3: its capacity to alter the cysteine redox state of the NOTCH3 ectodomain. Synthetic peptides corresponding to this sequence (NOTCH3 N-terminal fragment 2, NTF2) readily reduce NOTCH3 N-terminal ectodomain polypeptides in a dose- and time-dependent fashion. Furthermore, NTF2 preferentially reduces regional domains of NOTCH3 with the highest intensity against EGF-like domains 12–15. This process requires cysteine residues of NTF2 and is also capable of targeting selected extracellular proteins that include TSP2 and CTSH. CADASIL mutations in NOTCH3 increase susceptibility to NTF2-facilitated reduction and to trans-reduction by NOTCH3 produced in cells. Moreover, NTF2 forms complexes with the NOTCH3 ectodomain, and cleaved NOTCH3 co-localizes with the NOTCH3 ectodomain in cerebral arteries of CADASIL patients. The potential for NTF2 to reduce vascular proteins and the enhanced preference for it to trans-reduce mutant NOTCH3 implicate a role for protein trans-reduction in cerebrovascular pathological states such as CADASIL.     

高强聚烯烃静态断裂过程银纹区的性能1.HDPE

为了观察分析高密度聚乙烯(HDPE)的银纹区性能,对两种HDPE全切痕试样分别进行了速率为0.1 mm/min,0.5 mm/min,1 mm/min,5 mm/min,20 mm/min和50 mm/min的拉伸测试。基于对静态断裂过程应力-位移数据的分析处理,结果表明,HDPE的屈服应力随着拉伸速率增大;高应力时两种HDPE均存在常临界位移控制其银纹断裂;HDPE2480银纹微纤的稳定性较大,材料韧性较好。HDPE静态拉伸断面显微结构显示,HDPE2480的银纹微纤尺寸大于HDPE4801,导致两者断面弧形巢尺寸和弧形巢内粗糙度的不同。     

Extension of the applicable range of the implicit curve-fitting method for refrigerant thermodynamic properties to critical pressure

The method of implicit curve-fitting and explicit-calculation has been used for fast and stable calculations of thermodynamic properties of subcritical refrigerants. In order to extend that method to the critical pressure, a method of sectional implicit curve-fitting and explicit-calculation for refrigerant thermodynamic properties is introduced in this paper. The whole data range is divided into several subsections. The requirements on the continuity of thermodynamic properties and the first order derivative of thermodynamic properties in the intersection points of subsections are indicated, and the methods to meet the requirements are presented. Quadric equations are constructed instead of curve-fitting when no data can be given. With the source data obtained from REFPROP 7.1, explicit fast calculation formulae for thermodynamic properties of R410A, covering the saturated temperature of 213.15–344.51 K and superheat of 0–65 K, are given as an example. The calculation speeds of the formulae of R410A are more than 7000 times faster than those of REFPROP 7.1 while the total mean relative deviation of the fast calculation formulae from REFPROP 7.1 is only 0.04%.     

Design study of APS-U-type hybrid-MBA lattice for mid-energy DLSR

In recent years,a new generation of storage ring-based light sources,known as diffraction-limited storage rings(DLSRs),whose emittance approaches the diffractio...