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81.
The mouse GABA transporter (mGAT1) gene has been shown to be exclusively expressed in brain by Northern and Western blot analyses. The interactions between the 5' flanking region of the mGAT1 gene and nuclear proteins from different mouse tissues were studied by means of gel-shift assay. Our results show that nuclear protein factors from non-nervous tissues can specifically recognize a 37 bp sequence that is conserved in the 5' flanking region between the human and mouse GAT1 genes. Similar nuclear protein factors were also found to exist in rat, rabbit and pig. 相似文献
82.
Proadrenomedullin N-terminal 20 peptide (PAMP-[1-20]; ARLDVASEFRKKWNKWALSR-amide) is a potent hypotensive and catecholamine release-inhibitory peptide released from chromaffin cells. We studied the mechanism of PAMP action and how its function is linked to structure. We tested human PAMP-[1-20] on catecholamine secretion in PC12 pheochromocytoma cells and found it to be a potent, dose-dependent (IC50 approximately 350 nmol/L) secretory inhibitor. Inhibition was specific for nicotinic cholinergic stimulation since PAMP-[1-20] failed to inhibit release by agents that bypass the nicotinic receptor. Nicotinic cationic (22Na+,45Ca2+) signal transduction was disrupted by this peptide, and potencies for inhibition of 22Na+ uptake and catecholamine secretion were comparable. Even high-dose nicotine failed to overcome the inhibition, suggesting noncompetitive nicotinic antagonism. N- and C-terminal PAMP truncation peptides indicated a role for the C-terminal amide and refined the minimal active region to the C-terminal 8 amino acids (WNKWALSR-amide), a region likely to be alpha-helical. PAMP also blocked (EC50 approximately 270 nmol/L) nicotinic cholinergic agonist desensitization of catecholamine release, as well as desensitization of nicotinic signal transduction (22Na+ uptake). Thus, PAMP may exert both inhibitory and facilitatory effects on nicotinic signaling, depending on the prior state of nicotinic stimulation. PAMP may therefore contribute to a novel, autocrine, homeostatic (negative-feedback) mechanism controlling catecholamine release. 相似文献
83.
84.
The best prototype for designing a new user interface is your old user interface. The second best prototype is a competing product. Your competitors have invested significant resources in designing and implementing what they believe to be good user interfaces. You can glean much of what you need to create a new interface by examining products designed to solve similar problems. As with your own old user interface, you can analyze competing interfaces to see what works and what doesn't. You can also watch how users interact with competing products, and thus learn how they approach tasks. This, in essence, is competitive usability analysis. I recommend performing it very early in the usability engineering life-cycle-after you have visited the customer, gathered requirements, and defined the product vision, but before you design and prototype your new user interface 相似文献
85.
L. H. Lang D. C. Kang S. H. Zhang Z. R. Wang S. J. Yuan K. B. Nielsen J. Dancket ) Department of Materials Engineering Harbin Institute of Technology Harbin China ) Aalberg University Denmark 《金属学报(英文版)》2000,13(2):476-480
1.~nonHydridapawhcdeepdrawing(HDD)belongstothefieldofsheetmetalfoeingandcanbeaPPliedinthefieldofautomotive,aerospace,drilitaryindustryetc.Ithasbeenrecognizedwidelyintheworld["'j.Fig.Ishowsitsprinciple:TheliqUidthatisfilledinthe~ngdiecavitywillbepressedwhenthepunchgoesdownsothattheeffectoffrictionkeepingandoverflowlubricatingcanbeformed,astherestilt,thefoeinglicitofsheetmetalwillbeimprovedareailvandthegualitVofthefinishedpartswillbeup~ed.BecauseofmanyadvantagesofHDDprocees,ithasbeenwidelys… 相似文献
86.
Simulation of nitrogen dynamics and biomass production in winter wheat using the Danish simulation model DAISY 总被引:14,自引:0,他引:14
S. Hansen H. E. Jensen N. E. Nielsen H. Svendsen 《Nutrient Cycling in Agroecosystems》1991,27(2-3):245-259
A dynamic simulation model for the soil plant system is described. The model includes a number of main modules, viz., a hydrological model including a submodel for soil water dynamics, a soil temperature model, a soil nitrogen model including a submodel for soil organic matter dynamics, and a crop model including a submodel for nitrogen uptake. The soil part of the model has a one-dimensional vertical structure. The soil profile is divided into layers on the basis of physical and chemical soil characteristics. The simulation model was used to simulate soil nitrogen dynamics and biomass production in winter wheat grown at two locations at various levels of nitrogen fertilization. The simulated results were compared to experimental data including concentration of inorganic nitrogen in soil, crop yield, and nitrogen accumulated in the aboveground part of the crop. Based on this validation it is concluded that the overall performance of the model is satisfactory although some minor adjustments of the model may prove to be necessary. 相似文献
87.
Anna Puig-Molina Lars Pleth Nielsen Alfons M. Molenbroek Konrad Herbst 《Catalysis Letters》2004,92(1-2):29-34
The chemical state of arsenic deposited on a NiMoP/Al2O3 hydrotreating catalyst exposed to ppb levels of arsenic over several years in a refinery reactor has been studied by in situ EXAFS. In the as-received As-NiMoP catalyst, arsenic is exclusively coordinated to oxygen atoms. Upon sulfiding the sample in 2%H2S/2%H2/96%He, the As atoms become surrounded by approximately two sulfur atoms. No evidence was found for Ni–As bond formation. A possible model for the As local environment is suggested on the basis of combined EXAFS results, STM data and FEFF8.0 simulations (program for ab initio calculations on multiple scattering XAFS and XANES). The FEFF8.0 simulations of the proposed model are in accord with the experimental data measured at the As K edge. In this model, an As atom is located at the edge of a hexagonally truncated Ni-MoS2 slab and is blocking the active NiMoS site. 相似文献
88.
Nielsen Per F.; Roepstorff Peter; Clausen Ib G.; Jensen Ejner B.; Jonassen Ib; Svendsen Allan; Balschmidt Per; Hansen Finn B. 《Protein engineering, design & selection : PEDS》1989,2(6):449-457
Californium-252 plasma desorption mass spectrometry (PDMS) hasbeen employed for the characterization of a series of humaninsulin derivatives in order to evaluate the performance ofthis technique as an analytical tool in protein engineering.Several of the characterized modifications result in a 1 a.m.u.mass change. The precision in mass determination obtainableby PDMS analysis is not sufficient for unambiguous verificationof such modifications based on the molecular weight alone. Itis, however, possible to carry out in situ enzymatic digestionof the sample. Subsequent PDMS analysis will in most cases revealif the modification has been introduced as intended. 相似文献
89.
Nitrate leaching as affected by cropping system/crop rotation, history of farmyard manure application or fertilizer nitrogen application (0 N, 0.5 N and 1 N) was studied at nine sites on loamy soils during 1986/87, 1987/88 and 1988/89. Soil solution from 80 to 90 cm soil depth was sampled every second week in the period November to May by the use of porous ceramic cups and analysed for NO3-N and Cl. Climatical conditions were obtained from standard meteorological observations in the region. Drainage from soil profiles was calculated from measured and simulated values of precipitation and actual evapotranspiration, respectively.The results show that type of crop is of the utmost importance for the leaching magnitude of nitrate as 40% of the total variance in nitrate concentrations in the soil solution could be explained by the type of crop.The second factor of importance was the history of farmyard manure (FYM) application, which was able to explain 28% of the total variation in nitrate concentration in the soil solution. Nitrate concentration/leaching from arable land without FYM ever being applied was considerably lower than from arable land which received periodical FYM applications until the early 70's or from arable land which besides periodical FYM applications in the past presently still receives regular applications of FYM. Only about 1% of the total variation in nitrate concentration in the soil solution was explainable by the level of fertilizer nitrogen application.Differences between years explained 14% of the total variation in nitrate concentration in the soil solution due to differences between the years in soil temperatures and water run-off. The run-off during the autumn and winter of 1986/87, 1987/88 and 1988/89 was 95, 275 and 55 mm, respectively. As expected nitrate leaching increased with increasing run off. However, nitrate leaching at the 275 mm run off was considerably lower than expected, which seems explainable by a substantial denitrification. The soil at the sites in question seems thus partly to purify the soil solution for nitrate before it leaves the root zone at the observed high run off conditions. 相似文献
90.
Germplasm with shorter duration than that of the currently grown varieties is being generated to maximize productivity of irrigated rice. However, short-duration varieties often produce yields lower than the medium- and long-duration varieties. Experiments were conducted during the 1980–82 dry and wet seasons to increase productivity through the use of very early-maturing rices and the improved management of nitrogen (N) fertilizers.Results over three years showed that IR58 and IR9729-67-3 (growth duration 100 ± 5 days) yield as well as or higher than IR36 although earlier maturing. They generally had a higher productivity (kg ha–1 day–1) than IR36 (110 ± 5 days).Three years' data suggest that the improved timing of broadcast applications of urea in split doses increased grain yield comparable with the basal incorporation of slow-release sulfur-coated urea (SCU) or deep point-placement of urea supergranules (USG).Results on elite breeding lines showed that the early-maturing IR9729-67-3 produced higher protein yield ha–1 than longer duration varieties such as IR8 and IR42 in the dry season. Furthermore, contrary to earlier results, single basal incorporation of slow-release SCU increased the protein yield of rice by 53 kg ha–1 and deep point-placement of USG by 43 kg ha–1 over split application of prilled urea. 相似文献