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131.
This paper presents an axisymmetric three-dimensional semi-empirical model that describes the airflow in both the spout and annular regions. Both the pressure and air velocity distributions can be calculated at any point inside the dryer. Thus, the effect of the fluid dynamics on the heat and mass transfer coefficients and, consequently, on the drying process, can be evaluated. The finite element method was used to solve the model. Pressure distributions were measured for several conditions in which stable spouted bed flow regime was attained. Simulated pressure distributions agreed well with experimental data. 相似文献
132.
CL Schmidt OM Hatzfeld A Petersen TA Link G Sch?fer 《Canadian Metallurgical Quarterly》1997,234(1):283-287
The Rieske protein II (Schmidt et al., 1996, FEBS Lett. 388, 43-46) from the thermoacidophilic crenarcheon Sulfolobus acidocaldarius (DSM 639) was expressed in E. coli cells. The full length protein was strictly bound to the E. coli membranes and could only be removed by detergent treatment indicating the presence of a membrane anchor. The iron sulfur cluster was correctly inserted into a fraction of the full length protein and much more effectively into a soluble form created by the deletion of the 45 N-terminal amino acids. The soluble form of the protein displayed the typical spectroscopic properties of a respiratory Rieske protein. The midpoint potential was +375 mV determined by CD redox potentiometry. The presented data demonstrate that the structure of the recombinant protein is very similar or identical to the authentic protein making this a powerful model system for the studies of Rieske proteins by site directed mutagenesis. 相似文献
133.
We describe a general method for plasmid assembly that uses yeast and extends beyond yeast-specific research applications. This technology exploits the homologous recombination, double-stranded break repair pathway in Saccharomyces cerevisiae to join DNA fragments. Synthetic, double-stranded "recombination linkers" were used to "subclone" a DNA fragment into a plasmid with > 80% efficiency. Quantitative data on the influence of DNA concentration and overlap length on the efficiency of recombination are presented. Using a simple procedure, plasmids were shuttled from yeast into E. coli for subsequent screening and large-scale plasmid preps. This simple method for plasmid construction has several advantages. (i) It bypasses the need for extensive PCR amplification and for purification, modification and/or ligation techniques routinely used for plasmid constructions. (ii) The method does not rely on available restriction sites, thus fragment and vector DNA can be joined within any DNA sequence. This enables the use of multifunctional cloning vectors for protein expression in mammalian cells, other yeast species, E. coli and other expression systems as discussed. (iii) Finally, the technology exploits yeast strains, plasmids and microbial techniques that are inexpensive and readily available. 相似文献
134.
Placental expression of the alpha subunit gene of the human glycoprotein hormones requires a multicomponent enhancer composed of tandem cAMP-response elements and an adjacent upstream regulatory element. Based on recent studies indicating that the upstream regulatory element includes binding sites for more than one protein, we investigated how functional activity correlated with these binding sites. Through extensive replacement mutagenesis of the native promoter regulatory region, we provide the first functional map of the upstream regulatory element. Within this region, we find that distinct proteins interact with three overlapping binding sites. While each site is functionally significant, no single site is essential or displays clear dominance. This is surprising since one of the sites binds a placenta-specific protein that heretofore has been regarded as essential for activity of the human alpha subunit placenta-specific enhancer. Consequently, our refined functional map of the upstream regulatory element reveals a complex combinatorial code that directs expression of the human alpha subunit gene to placenta. 相似文献
135.
在准备金评估中,由于多数随机准备金模型基本假设的限制是不能直接处理含有零值和负值增量赔款三角形,本文利用一个变换和广义线性模型的混合模型去解决这种情况. 相似文献
136.
为了加深对盆地深部和浅部高自然伽马异常特征综合分析研究,揭示高自然伽马异常地质成因,从而
促进盆地多种能源矿产协同开发研究,针对收集大量的地球物理测井资料、地质资料及其他相关成果,绘制并分
析了相关成果图。研究中遵循从单孔测井曲线特征分析到典型剖面研究,并按层对高自然伽马的平面分布特征
进行分析研究。化验测试结果和自然伽马能谱测井成果研究表明,在本区,自然伽马异常增大主要是由于铀的
活化和聚集,导致铀元素增加而引起。相关研究表明,盆地深部和浅部的铀异常具有一定的关系,盆地历经的沉
积环境变迁是铀元素富集的主导因素。 相似文献
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140.
Wall shear stress in postcapillary venules varies widely within and between tissues and in response to inflammation and exercise. However, the speed at which leukocytes roll in vivo has been shown to be almost constant within a wide range of wall shear stress, i.e., force on the cell. Similarly, rolling velocities on purified selectins and their ligands in vitro tend to plateau. This may be important to enable rolling leukocytes to be exposed uniformly to activating stimuli on endothelium, independent of local hemodynamic conditions. Wall shear stress increases the rate of dissociation of individual selectin-ligand tether bonds exponentially (, ) thereby destabilizing rolling. We find that this is compensated by a shear-dependent increase in the number of bonds per rolling step. We also find an increase in the number of microvillous tethers to the substrate. This explains (a) the lack of firm adhesion through selectins at low shear stress or high ligand density, and (b) the stability of rolling on selectins to wide variation in wall shear stress and ligand density, in contrast to rolling on antibodies (). Furthermore, our data successfully predict the threshold wall shear stress below which rolling does not occur. This is a special case of the more general regulation by shear of the number of bonds, in which the number of bonds falls below one. 相似文献