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81.
82.
83.
Oreshkin EF Borisova MA Tchubarova GS Gorbatov VM Permyakov EA Shnyrov VL Burstein EA 《Meat science》1986,16(4):297-305
Structural changes caused by heating in the proteins of cured beef longissimus dorsi muscle were examined by fluorescence and differential scanning calorimetry. Denaturation occurred in four temperature ranges—40–55°C, 55–61°C, 62–70°C and above 70°C, in contrast to the three endothermic transitions reported for non-cured meats. 相似文献
84.
A. R. Pavlov P. P. Permyakov A. V. Stepanov 《Journal of Engineering Physics and Thermophysics》1980,39(2):885-889
The article explains an algorithm for determining the thermophysical characteristics of dispersed media with phase transitions based on the method of solving inverse problems of heat conduction.Notation r
space coordinate
-
time
- T
temperature of the specimen
- T0
initial temperature
- ci, cw, csk
specific heat of ice, water, and of the organic-mineral skeleton, respectively
- cf, cm, f, m
specific heat and thermal conductivity in the frozen and melted zones, respectively
- c
effective heat capacity
-
thermal conductivity
-
p
density
- 0, sb
bound and strongly bound moisture, respectively
- (T)
amount of nonfrozen water
- R
radius of the cylinder
- q()
heat flux
- I
functional
- u1(), U2()
measured temperatures of the specimen at the points r = 0 and r = R, respectively, at the instant
- 1, 2
degree of confidence of the supplementary information
-
final instant of time
- a, b, k, s
positive constants
- L
specific heat of melting
- N
number of grid nodes over space
- n
number of grid nodes over time
- h
grid step over space
-
grid step over time
-
solution of the conjugate system
- s
number of iteration
Translated from Inzhenerno-Fizicheskii Zhurnal, Vol. 39, No. 2, pp. 292–297, August, 1980. 相似文献
85.
Interactions of (Ala*Ala*Lys*Pro)n and (Lys*Lys*Ser*Pro)n with DNA. Proposed coiled-coil structure of AlgR3 and AlgP from Pseudomonas aeruginosa 总被引:1,自引:0,他引:1
Medvedkin Vyacheslav N.; Permyakov Eugene A.; Klimenko Lyubov V.; Mitin Yuri V.; Matsushima Norio; Nakayama Susumu; Kretsinger Robert H. 《Protein engineering, design & selection : PEDS》1995,8(1):63-70
The proteins, AlgR3 and AlgP, are involved in the regulationof alginate synthesis in Pseudomonas. They contain multiplerepeats of Ala*Ala*Lys*Pro as do several other proteins thatresemble histones. The interactions of synthesis oligopeptidescomposed of repeated Ala*Ala*Lys*Pro or Lys*Lys*Ser*Pro unitswith DNA were studied by fluorescence of the Fmoc (9-fluorenylmethyloxycarbonyl)group attached to the N-termini of the peptides. DNA quenchingof the Fmoc fluorescence of the peptides was used to estimatethe apparent association constants for the interaction of Fmoc(AAKP)nOH(n = 2, 4, 8, 18, 32) and of Fmoc(KKSP)nOH (n = 2, 4, 8, 16,20, 32) with DNA. The Fmoc(AAKP)nOH peptides bind to DNA onlyat low ionic strength; the Fmoc(KKSP)n OH peptides interactwith DNA at both low (0.05 M KCl) and high (0.2 M KCl) saltAt low ionic strength an increase in the number of the repeatunits causes an increase in the apparent association constantup to {small tilde}2 x 106 M1 for both types of peptidesat N 24. The insertion of an AAKTA unit into the middle ofthe Fmoc(AAKP)8OH peptide increases its affinity to DNA. Wepropose a model of (AAKP)n and of its interaction with DNA.The repeat unit consists of a single turn of -helix followedby a bend necessitated by Pro. The resultant coiled-coil formsa right-handed superhelix with 10 AAKPs per repeat distanceof {small tilde}33 Å. With only slight modification ofthe canonical parameters of this model the AAKP super helixfits into the major groove of B-form DNA with one AAKP tetramerper base pair repeat of 3.4 Å. The -amine nitrogen ofLys can form a polar hydrogen bond with a phosphate oxygen atomof the DNA backbone. A better fit is obtained when the modelis modified to accommodate [(AAKP)5AAKTA]n as actually observedin AlgR3. We suggest that this coiled-coil represents a generalmotif for other proteinDNA interactions. 相似文献
86.
Olga A. Averina Ivan G. Laptev Mariia A. Emelianova Oleg A. Permyakov Sofia S. Mariasina Alyona I. Nikiforova Vasily N. Manskikh Olga O. Grigorieva Anastasia K. Bolikhova Gennady A. Kalabin Olga A. Dontsova Petr V. Sergiev 《International journal of molecular sciences》2022,23(11)
Mitochondrial translation is a unique relic of the symbiotic origin of the organelle. Alterations of its components cause a number of severe human diseases. Hereby we report a study of mice devoid of Mettl15 mitochondrial 12S rRNA methyltransferase, responsible for the formation of m4C839 residue (human numbering). Homozygous Mettl15−/− mice appeared to be viable in contrast to other mitochondrial rRNA methyltransferase knockouts reported earlier. The phenotype of Mettl15−/− mice is much milder than that of other mutants of mitochondrial translation apparatus. In agreement with the results obtained earlier for cell cultures with an inactivated Mettl15 gene, we observed accumulation of the RbfA factor, normally associated with the precursor of the 28S subunit, in the 55S mitochondrial ribosome fraction of knockout mice. A lack of Mettl15 leads to a lower blood glucose level after physical exercise relative to that of the wild-type mice. Mettl15−/− mice demonstrated suboptimal muscle performance and lower levels of Cox3 protein synthesized by mitoribosomes in the oxidative soleus muscles. Additionally, we detected decreased learning capabilities in the Mettl15−/− knockout mice in the tests with both positive and negative reinforcement. Such properties make Mettl15−/− knockout mice a suitable model for mild mitochondriopathies. 相似文献
87.
Maksimov P. V. Muratov K. R. Ablyaz T. R. Fetisov K. V. Permyakov M. S. 《Russian Engineering Research》2021,41(5):469-471
Russian Engineering Research - Prediction of part technological distortions on the basis of the computer simulation of additive manufacturing (selective laser melting) is considered. The modeling... 相似文献
88.
Generic Structures of Cytotoxic Liprotides: Nano‐Sized Complexes with Oleic Acid Cores and Shells of Disordered Proteins 下载免费PDF全文
Dr. Jørn D. Kaspersen Jannik N. Pedersen Dr. Jon G. Hansted Dr. Søren B. Nielsen Srinivasan Sakthivel Dr. Kristina Wilhelm Dr. Ekaterina L. Nemashkalova Prof. Dr. Sergei E. Permyakov Prof. Dr. Eugene A. Permyakov Prof. Dr. Cristiano Luis Pinto Oliveira Prof. Dr. Ludmilla A. Morozova‐Roche Prof. Dr. Daniel E. Otzen Prof. Dr. Jan Skov Pedersen 《Chembiochem : a European journal of chemical biology》2014,15(18):2693-2702
The cytotoxic complex formed between α‐lactalbumin and oleic acid (OA) has inspired many studies on protein–fatty acid complexes, but structural insight remains sparse. After having used small‐angle X‐ray scattering (SAXS) to obtain structural information, we present a new, generic structural model of cytotoxic protein–oleic acid complexes, which we have termed liprotides (lipids and partially denatured proteins). Twelve liprotides formed from seven structurally unrelated proteins and prepared by different procedures all displayed core–shell structures, each with a micellar OA core and a shell consisting of flexible, partially unfolded protein, which stabilizes the OA micelle. The common structure explains similar effects exerted on cells by different liprotides and is consistent with a cargo off‐loading of the OA into cell membranes. 相似文献
89.
90.
B. M. Ginzburg D. G. Tochil’nikov A. I. Lyashkov P. A. Shiyan V. M. Gaisin I. V. Permyakov V. P. Ponimatkin 《Journal of Friction and Wear》2011,32(4):246-250
The paper presents the results of testing of block specimens of polyoxymethylene in water-lubricated sliding boundary friction
against steel. Under these testing conditions, the polymer shows a combination of good antiwear and antifriction properties.
With the addition of 1 wt % of fullerene soot to the polyoxymethylene, its wear rate declines considerably, while its friction
coefficient remains quite low; its carrying capacity is high and the wear process is stable. The friction coefficient, wear
rate, and carrying capacity of modified pilyoxymethylene are compared to those of carbon plastics under similar testing conditions. 相似文献