全文获取类型
收费全文 | 3518篇 |
免费 | 183篇 |
国内免费 | 6篇 |
专业分类
电工技术 | 52篇 |
综合类 | 3篇 |
化学工业 | 982篇 |
金属工艺 | 76篇 |
机械仪表 | 86篇 |
建筑科学 | 139篇 |
矿业工程 | 6篇 |
能源动力 | 154篇 |
轻工业 | 266篇 |
水利工程 | 28篇 |
石油天然气 | 3篇 |
无线电 | 353篇 |
一般工业技术 | 697篇 |
冶金工业 | 177篇 |
原子能技术 | 32篇 |
自动化技术 | 653篇 |
出版年
2023年 | 28篇 |
2022年 | 80篇 |
2021年 | 105篇 |
2020年 | 55篇 |
2019年 | 76篇 |
2018年 | 86篇 |
2017年 | 92篇 |
2016年 | 120篇 |
2015年 | 104篇 |
2014年 | 144篇 |
2013年 | 241篇 |
2012年 | 220篇 |
2011年 | 281篇 |
2010年 | 182篇 |
2009年 | 217篇 |
2008年 | 226篇 |
2007年 | 168篇 |
2006年 | 179篇 |
2005年 | 124篇 |
2004年 | 107篇 |
2003年 | 103篇 |
2002年 | 120篇 |
2001年 | 55篇 |
2000年 | 51篇 |
1999年 | 49篇 |
1998年 | 66篇 |
1997年 | 70篇 |
1996年 | 40篇 |
1995年 | 32篇 |
1994年 | 36篇 |
1993年 | 26篇 |
1992年 | 17篇 |
1991年 | 15篇 |
1990年 | 20篇 |
1989年 | 17篇 |
1988年 | 9篇 |
1987年 | 8篇 |
1986年 | 13篇 |
1985年 | 15篇 |
1984年 | 17篇 |
1983年 | 9篇 |
1982年 | 10篇 |
1981年 | 14篇 |
1980年 | 7篇 |
1979年 | 7篇 |
1978年 | 8篇 |
1977年 | 5篇 |
1976年 | 9篇 |
1975年 | 5篇 |
1972年 | 4篇 |
排序方式: 共有3707条查询结果,搜索用时 15 毫秒
61.
Grouped data occur frequently in practice, either because of limited resolution of instruments, or because data have been summarized in relatively wide bins. A combination of the composite link model with roughness penalties is proposed to estimate smooth densities from such data in a Bayesian framework. A simulation study is used to evaluate the performances of the strategy in the estimation of a density, of its quantiles and first moments. Two illustrations are presented: the first one involves grouped data of lead concentration in the blood and the second one the number of deaths due to tuberculosis in The Netherlands in wide age classes. 相似文献
62.
63.
64.
Polymerization of β-butyrolactone has been studied in toluene with Al(OiPr)3 as an initiator. The ring-opening polyaddition proceeds through a coordination–insertion mechanism at a very low rate. Well defined α-isopropylester, ω-hydroxy poly(β-butyrolactone)s (PBL) are formed with a narrow molecular weight distribution at low monomer-to-initiator molar ratios, When this ratio is higher (ca. 170), a competition occurs between propagation and side reactions, i.e. elimination, inter- and intra-molecular transesterifications and thermal degradation, which is responsible for a loss of control of the PBL molecular charcteristics. The addition of a Lewis base (1 equivalent of nicotine/Al) to the Al-alkoxide initiator has no significant effect on the polymerization rate, although the chain microstructure is deeply affected since predominantly syndiotactic PBL chains are formed (63% syndio-diads) in contrast to a completely atactic polymer in the absence of nicotine. 相似文献
65.
Jerome Kluza Victoriane Peugnet Blanche Daunou William Laine Gwenola Kervoaze Gaëlle Rmy Anne Loyens Patrice Maboudou Quentin Fovez Corinne Grangette Isabelle Wolowczuk Philippe Gosset Guillaume Garon Philippe Marchetti Florence Pinet Muriel Pichavant Emilie Dubois-Deruy 《International journal of molecular sciences》2022,23(1)
Mitochondrial dysfunctions are implicated in several pathologies, such as metabolic, cardiovascular, respiratory, and neurological diseases, as well as in cancer and aging. These metabolic alterations are usually assessed in human or murine samples by mitochondrial respiratory chain enzymatic assays, by measuring the oxygen consumption of intact mitochondria isolated from tissues, or from cells obtained after physical or enzymatic disruption of the tissues. However, these methodologies do not maintain tissue multicellular organization and cell-cell interactions, known to influence mitochondrial metabolism. Here, we develop an optimal model to measure mitochondrial oxygen consumption in heart and lung tissue samples using the XF24 Extracellular Flux Analyzer (Seahorse) and discuss the advantages and limitations of this technological approach. Our results demonstrate that tissue organization, as well as mitochondrial ultrastructure and respiratory function, are preserved in heart and lung tissues freshly processed or after overnight conservation at 4 °C. Using this method, we confirmed the repeatedly reported obesity-associated mitochondrial dysfunction in the heart and extended it to the lungs. We set up and validated a new strategy to optimally assess mitochondrial function in murine tissues. As such, this method is of great potential interest for monitoring mitochondrial function in cohort samples. 相似文献
66.
Retrogradation rate and extent were determined by differential scanning calorimetry and rheology measurements on 40% dry matter gels made from pea, modified waxy maize, rice, waxy rice, wheat, manioc, potato and microwaved irradiated potato starches. As a result, each starch retrogrades differently, depending also on the measurement technique. Nevertheless, potato and pea starches seem to be the most sensitive and the waxy and modified waxy types are the least sensitive to retrogradation. 相似文献
67.
Peter T.Cummings Philippe M.Fauchet Michael Goldfarb Martha W.M.Jones Maithilee Kunda Jonathan B.Perlin Nilanjan Sarkar Keivan G.Stassun Zachary E.Warren Karl E.Zelik 《工程(英文)》2021,7(2):141-143,中插10-中插13
1. Background
The use of engineering tools, design, research, and thinking to create environments and capabilities whereby individuals who are currently under-e... 相似文献
68.
Alexandre Joushomme Andr Garenne Mlody Dufosse Rmy Renom Hermanus Johannes Ruigrok Yann Loick Chappe Anne Canovi Lorenza Patrignoni Annabelle Hurtier Florence Poulletier de Gannes Isabelle Lagroye Philippe Lvêque Noëlle Lewis Muriel Priault Delia Arnaud-Cormos Yann Percherancier 《International journal of molecular sciences》2022,23(2)
It remains controversial whether exposure to environmental radiofrequency signals (RF) impacts cell status or response to cellular stress such as apoptosis or autophagy. We used two label-free techniques, cellular impedancemetry and Digital Holographic Microscopy (DHM), to assess the overall cellular response during RF exposure alone, or during co-exposure to RF and chemical treatments known to induce either apoptosis or autophagy. Two human cell lines (SH-SY5Y and HCT116) and two cultures of primary rat cortex cells (astrocytes and co-culture of neurons and glial cells) were exposed to RF using an 1800 MHz carrier wave modulated with various environmental signals (GSM: Global System for Mobile Communications, 2G signal), UMTS (Universal Mobile Telecommunications System, 3G signal), LTE (Long-Term Evolution, 4G signal, and Wi-Fi) or unmodulated RF (continuous wave, CW). The specific absorption rates (S.A.R.) used were 1.5 and 6 W/kg during DHM experiments and ranged from 5 to 24 W/kg during the recording of cellular impedance. Cells were continuously exposed for three to five consecutive days while the temporal phenotypic signature of cells behavior was recorded at constant temperature. Statistical analysis of the results does not indicate that RF-EMF exposure impacted the global behavior of healthy, apoptotic, or autophagic cells, even at S.A.R. levels higher than the guidelines, provided that the temperature was kept constant. 相似文献
69.
Xavier Vanhoye Alexandre Janin Amandine Caillaud Antoine Rimbert Fabienne Venet Morgane Gossez Wieneke Dijk Oriane Marmontel Sverine Nony Charlotte Chatelain Christine Durand Pierre Lindenbaum Jennifer Rieusset Bertrand Cariou Philippe Moulin Mathilde Di Filippo 《International journal of molecular sciences》2022,23(8)
Hypobetalipoproteinemia is characterized by LDL-cholesterol and apolipoprotein B (apoB) plasma levels below the fifth percentile for age and sex. Familial hypobetalipoproteinemia (FHBL) is mostly caused by premature termination codons in the APOB gene, a condition associated with fatty liver and steatohepatitis. Nevertheless, many families with a FHBL phenotype carry APOB missense variants of uncertain significance (VUS). We here aimed to develop a proof-of-principle experiment to assess the pathogenicity of VUS using the genome editing of human liver cells. We identified a novel heterozygous APOB-VUS (p.Leu351Arg), in a FHBL family. We generated APOB knock-out (KO) and APOB-p.Leu351Arg knock-in Huh7 cells using CRISPR-Cas9 technology and studied the APOB expression, synthesis and secretion by digital droplet PCR and ELISA quantification. The APOB expression was decreased by 70% in the heterozygous APOB-KO cells and almost abolished in the homozygous-KO cells, with a consistent decrease in apoB production and secretion. The APOB-p.Leu351Arg homozygous cells presented with a 40% decreased APOB expression and undetectable apoB levels in cellular extracts and supernatant. Thus, the p.Leu351Arg affected the apoB secretion, which led us to classify this new variant as likely pathogenic and to set up a hepatic follow-up in this family. Therefore, the functional assessment of APOB-missense variants, using gene-editing technologies, will lead to improvements in the molecular diagnosis of FHBL and the personalized follow-up of these patients. 相似文献
70.
Nizard Philippe; Chenal Alexandre; Beaumelle Bruno; Fourcade Alain; Gillet Daniel 《Protein engineering, design & selection : PEDS》2001,14(6):439-446
We have shown previously that the diphtheria toxin transmembranedomain (T) may function as a membrane anchor for soluble proteinsfused at its C-terminus. Binding to membranes is triggered byacidic pH. Here, we further characterized this anchoring device.Soluble proteins may be fused at the N-terminus of the T domainor at both extremities, without modifying its membrane bindingproperties. This allows one to choose the orientation of theprotein to be attached to the membrane. Maximum binding to thecell surface is reached within 1 h. Anchoring occurs on cellspreviously treated with proteinase K, suggesting that T interactswith the lipid phase of the membrane without the help of cellsurface proteins. Binding does not permeabilize cells or affectcell viability, despite the fact that it permeabilizes liposomesand alters their structure. When attached to L929 fibroblasts,the proteins are not internalized and remain displayed at theirsurface for more than 24 h. When bound to K562 myeloid cells,the molecules are internalized and degraded. Thus, dependingon the cell type, soluble proteins may be anchored to the surfaceof cells by the T domain for an extended time or directed towardsan internalization pathway. 相似文献