无熟料高炉矿渣水泥对Cl-的抗渗特性研究

无熟料高炉矿渣水泥对Cl-的抗渗有独特的性能,为此,以石膏和石灰作为激发剂配制无熟料高炉矿渣水泥后,用电通量法等科学手段进行了Cl-的抗渗试验.主要介绍了试验采用的原材料及配合比,试验采用的方法及试验结果分析.结果表明,无熟料高炉矿渣水泥的效果远远好于硅酸盐水泥和矿渣水泥.     

面向Kinect运动数据的鲁棒足迹检测

目的 Kinect可实时获取运动数据且较传统的运动捕捉设备采集成本低廉,在运动数据捕捉方面得到了广泛应用。但Kinect获取的运动数据精度较低,现有运动数据处理算法难以适用。方法 针对运动数据处理的关键步骤足迹检测问题,提出面向Kinect运动数据的鲁棒足迹检测算法。首先使用自适应的双边滤波算法降低Kinect运动数据中的噪声;其次定义多种脚部运动特征并用于分类,优化分类效果;最后使用支持向量机(SVM)算法训练决策函数并用于足迹检测。结果 本文算法应用于多种类型运动数据后,可以有效地减少Kinect运动数据中的噪声,足迹检测的时间性能以及准确性良好,其中足迹检测的准确率比经典的基准线方法提高了10%左右,比K近邻方法提高了8%左右,检测一帧运动足迹的速度为K近邻方法的7倍左右。结论 对实验结果的分析证明算法具有良好的鲁棒性、时间性能以及准确率,可广泛应用于运动数据的处理之中。     

SHMT2 Induces Stemness and Progression of Head and Neck Cancer

Various enzymes in the one-carbon metabolic pathway are closely related to the development of tumors, and they can all be potential targets for cancer therapy. Serine hydroxymethyltransferase2 (SHMT2), a key metabolic enzyme, is very important for the proliferation and growth of cancer cells. However, the function and mechanism of SHMT2 in head and neck cancer (HNC) are not clear. An analysis of The Cancer Genome Atlas (TCGA) data showed that the expression of SHMT2 was higher in tumor tissue than in normal tissue, and its expression was significantly associated with male sex, aggressive histological grade, lymph node metastasis, distant metastasis, advanced TNM stage, and lymphovascular invasion in HNC. SHMT2 knockdown in FADU and SNU1041 cell lines significantly inhibited cell proliferation, colony formation, migration, and invasion. Additionally, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses using TCGA data revealed that SHMT2 was closely related to cancer stem cell regulation and maintenance. Furthermore, we found that silencing SHMT2 inhibited the expression of stemness markers and tumor spheroid formation compared with a control group. On the contrary, stemness markers were significantly increased after SHMT2 overexpression in HEP-2 cells. Interestingly, we found that knocking down SHMT2 reduced the expression of genes related to the Notch and Wnt pathways. Finally, silencing SHMT2 significantly reduced tumor growth and decreased stemness markers in a xenograft model. Taken together, our study suggests that targeting SHMT2 may play an important role in inhibiting HNC progression.     

Functional Characterization of Ubiquitination Genes in the Interaction of Soybean—Heterodera glycines

Ubiquitination is a kind of post-translational modification of proteins that plays an important role in plant response to biotic and abiotic stress. The response of soybean GmPUB genes to soybean cyst nematode (SCN, Heterodera glycines) infection is largely unknown. In this study, quantitative real-time PCR (qRT-PCR) was performed to detect the relative expression of 49 GmPUB genes in susceptible cultivar William 82 and resistant cultivar Huipizhi after SCN inoculation. The results show that GmPUB genes responded to cyst nematode infection at 1 day post-inoculation (dpi), 5 dpi, 10 dpi and 15 dpi. The expression levels of GmPUB16A, GmPUB20A, GmCHIPA, GmPUB33A, GmPUB23A and GmPUB24A were dramatically changed during SCN infection. Furthermore, functional analysis of these GmPUB genes by overexpression and RNAi showed that GmPUB20A, GmPUB33A and GmPUB24A negatively regulated soybean resistance under SCN stress. The results from our present study provide insights into the complicated molecular mechanism of the interaction between soybean and SCN.     

Effects of Exosomes Derived from Adipose-Derived Mesenchymal Stem Cells on Pyroptosis and Regeneration of Injured Liver

Although accumulating evidence indicates that exosomes have a positive therapeutic effect on hepatic ischemia–reperfusion injury (HIRI), studies focusing on the alleviation of liver injury by exosomes derived from adipose-derived mesenchymal stem cells (ADSCs-Exo) based on the inhibition of cell pyroptosis have not yet been reported. Exosomes contain different kinds of biologically active substances such as proteins, lipids, mRNAs, miRNAs, and signaling molecules. These molecules are widely involved in cell–cell communication, cell signal transmission, proliferation, migration, and apoptosis. Therefore, we investigated the positive effects exerted by ADSCs-Exo after hepatic ischemia–reperfusion with partial resection injury in rats. In this study, we found that the post-operative tail vein injection of ADSCs-Exo could effectively inhibit the expression of pyroptosis-related factors such as NLRP3, ASC, caspase-1, and GSDMD-N, and promote the expression of regeneration-related factors such as Cyclin D1 and VEGF. Moreover, we found that the above cellular activities were associated with the NF-κB and Wnt/β-catenin signaling pathways. According to the results, ADSCs and ADSCs-Exo can reduce pyroptosis in the injured liver and promote the expression of those factors related to liver regeneration, while they can inhibit the NF-κB pathway and activate the Wnt/β-catenin pathway. However, although adipose-derived mesenchymal stem cell (ADSC) transplantation can reduce liver injury, it leads to a significant increase in the pyroptosis-related protein GSDMD-N expression. In conclusion, our study shows that ADSCs-Exo has unique advantages and significance as a cell-free therapy to replace stem cells and still has a broad research prospect in the clinical diagnosis and treatment of liver injuries.     

华北紫荆关深断裂影象解译

紫荆关断裂最早由河北省地质局区调队命名于六十年代初期,是指位于河北省太行山东麓易县紫荆关至涞水县白涧地段,向北可延至岑南台,与沿河城断裂相接,向南伸至曲阳县灵山附近,走向北北东,倾向南东。断裂两侧地层发育和地质发展史有很大区别,故命名之。     

Delayed neuropathy and inhibition of soluble neuropathy target esterase following the administration of organophosphorus compounds to hens

BACKGROUND: Disulfide exchange reactions are catalyzed by thiol/disulfide oxidoreductases. These enzymes possess a thioredoxin fold and contain a catalytic disulfide with the sequence Cys-X-X-Cys at the N terminus of an alpha helix. Despite these similarities, the various members differ strongly in their redox potentials (-122 mV to -270 mV). Using the strong oxidant DsbA from Escherichia coli as a model system, we investigated whether the redox properties of these enzymes can be modulated rationally by exchange of the X-X dipeptide. RESULTS: The X-X dipeptide of DsbA (Cys30-Pro31-His32-Cys33) was exchanged by the dipeptides of eukaryotic protein disulfide isomerase (PDI; Gly-His), glutaredoxin (Pro-Tyr), and thioredoxin (Gly-Pro) from E. coli. All variants were less oxidizing than wild-type DsbA and their redox potentials were in the order of the related natural enzymes (DsbA > PDI > glutaredoxin > thioredoxin). The equilibrium constant between glutathione and the thioredoxin-like variant increased 1200-fold compared with wild-type DsbA. The variants also showed a strong increase in the pKa of the nucleophilic cysteine (Cys30). As for glutaredoxin and thioredoxin, the catalytic disulfide stabilized the corresponding variants while destabilizing wild-type DsbA and the PDI-like variant. CONCLUSIONS: The X-X dipeptide in the active site of thiol/disulfide oxidoreductases appears to be the main determinant of the redox properties of these enzymes. This empirical finding should be very useful for the design of new thiol/disulfide oxidoreductases with altered redox potentials and for studying the function of these enzymes in vivo.     

Power-efficient grating-based scanning optical delay line: time-domain configuration

A power-efficient, grating-based, time-domain rapid scanning optical delay line (TD-RSOD) suitable for optical coherence tomography (OCT) is presented. The 6 dB power efficiency of this TD-RSOD over conventional double-pass Fourier-domain RSOD (FD-RSOD) provides approximately 4 dB SNR advantage in a typical balanced-detection OCT. The scanning performance of TD-RSOD is comparable to that of FD-RSOD.     

A novel cesium hydrogen sulfate-zeolite inorganic composite electrolyte membrane for polymer electrolyte membrane fuel cell application

A new type of CsHSO₄-HZSM-5 inorganic composite electrolyte membrane is prepared by mechanically mixing CsHSO₄ (CHS) and nanometer-scale HZSM-5 zeolite powders. The effects of HZSM-5 on the crystallite structure, proton conductivity, and thermal stability of the CsHSO₄ electrolyte are investigated. Incorporation of HZSM-5 is found to significantly increase the low-temperature proton conductivity of the CsHSO₄ electrolyte, extending its operating temperature down to 100 °C. The composite electrolyte with 40 mol% HZSM-5 shows the highest proton conductivity in the measured temperature range. The low-temperature activation energy of the composite with 40 mol% HZSM-5 is lower than that of the CHS-SiO₂ composite. The improvement of the proton conductivity can be attributed to the enhanced interfacial interaction between the two phases. And the small HZSM-5 particles lead to a change in the bulk properties of the ionic salts. The melting point of the CHS-HZSM-5 composite electrolyte is lower than that of the pure CHS electrolyte. The CHS-HZSM-5 composite electrolyte is suitable for polymer electrolyte membrane fuel cells operated at 100-200 °C.