浅谈人事档案管理

随着我国经济、政治体制的不断改革与深入,我国的人事档案出现了许多新的问题。为了改善这种情况,使得人事档案管理工作能够顺利地开展、与时俱进,更好地为人们服务,笔者结合多年的档案管理工作的经验,为人事档案管理提出了几条建议。     

填海地区复杂地质条件下的混凝土灌注桩施工技术

本文以深圳市后海片区百丽大厦项目混凝土灌注桩为例,分析并介绍了填海造陆地区复杂地质条件下的混凝土灌注桩技术,希望为类似工程施工提供参考。     

喷涂聚脲弹性体改性的研究进展

综述了喷涂聚脲弹性体的改性方法,重点介绍了环氧树脂、有机硅、酚醛树脂、液态聚硫橡胶改性喷涂聚脲弹性体的反应机理及改性后材料的性能变化特点.利用不同材料改性的方法,喷涂聚脲弹性体除保留原有的性能优势外,收缩率还明显减小,附着力得到改善,耐温及耐化学介质等方面性能也得以提高,扩大了其应用范围.     

不同加工工艺对临安山核桃营养成分的影响

为探明不同加工工艺对临安山核桃营养成分的影响,运用高效液相色谱、气相色谱法分析其营养成分含量。结果表明,不同加工工艺对山核桃粗蛋白含量影响差异不显著;烘煮工艺、干炒工艺熟制山核桃氨基酸总量较生核桃分别下降14.86%和25.95%。其中人体必需氨基酸总量较生核桃分别下降19.69%和29.96%。干炒工艺较烘煮工艺熟制山核桃氨基酸总量下降13.02%,人体必需氨基酸下降12.79%;烘煮工艺、干炒工艺熟制山核桃分别较生山核桃不饱和脂肪酸含量下降6.43%和18.18%,饱和脂肪酸含量上升40.61%和114.70%。      

脐橙新型壳聚糖水凝胶抑菌保鲜研究

以纽荷尔脐橙为试材,研究了壳聚糖水凝胶对青霉菌(Penicillium italicum)的抑制及其对脐橙的保鲜效果。结果表明不同浓度壳聚糖水凝胶对脐橙采后P.italicum均有一定的抑制作用。当浓度为2.0%时,培养5d后,菌落直径15mm,抑菌率42%,抑菌圈直径65mm,且涂膜后贮藏90d品质最好。说明壳聚糖水凝胶对脐橙采后P.italicum具有明显抑制作用,其中以2.0%的壳聚糖水凝胶效果最优,同时能够提高脐橙贮藏品质。      

LC-MS/MS法测定小鼠脑组织中色氨酸及其3种代谢物

目的:建立检测小鼠脑组织中色氨酸、5-羟基色氨酸、5-羟色胺和5-羟基吲哚乙酸含量的LC-MS/MS分析方法。方法:采用TSK Gel amide 80（2.0 mm×15 cm,3μm）色谱柱,柱温35℃,乙腈:甲酸铵水溶液（15 mmol·L-1,p H5.5）（40∶60,v/v）为流动相,流速0.4 m L·min-1,采用正离子多反应监测（MRM）模式进行扫描。结果:4种待测物均在5 min内完成测定,色氨酸、5-羟基色氨酸、5-羟色胺和5-羟基吲哚乙酸最低定量限分别为0.2、0.1、0.2、0.2 ng·m L-1,最低检测限分别为0.05、0.02、0.05、0.05 ng·m L-1,在1⁵00 ng·m L-1浓度范围内线性关系均良好,方法的精密度和加样回收率均符合生物样品的分析要求。将该方法应用于口服萱草花总黄酮的小鼠脑提取物检测后发现:脑内四种待测物浓度明显发生改变,且变化规律与阳性药物帕罗西汀相同。结论:本方法简便、准确、灵敏度高,专属性强,重复性好,适用于脑组织中色氨酸-五羟色胺代谢过程中的4种成分的测定,可应用于神经精神类药物的药理作用机制研究。      

晋西北酸粥发酵过程中微生物基因组DNA提取方法的比较

为了找到一种简单高效的晋西北酸粥发酵过程中微生物基因组DNA提取方法,本文采取对提取的微生物基因组DNA进行浓度测定的方法,比较了溶菌酶-SDS-蛋白酶K法、改良CTAB法和改良CTAB-溶菌酶法三种方法的提取效果。结果表明:溶菌酶-SDS-蛋白酶K法效果最好,可以获得质量较高的微生物基因组DNA,DNA浓度为1657.53 ng/μL;改良CTAB-溶菌酶法比溶菌酶-SDS-蛋白酶K法效果差,DNA浓度为1308.08 ng/μL;改良CTAB法对微生物基因组DNA的提取效果比溶菌酶-SDS-蛋白酶K法更差,DNA浓度为1098.36 ng/μL。以提取到的微生物基因组总DNA为模板,进行16S r DNA基因的PCR扩增,在回收产物中目的条带清晰,表明提取到的微生物基因组DNA含量高、结构完整。整体实验结果表明,溶菌酶-SDS-蛋白酶K法对于提取晋西北酸粥发酵过程中微生物基因组DNA效果最好。      

Autophagy: A Double-Edged Sword in Male Reproduction

Autophagy, an evolutionarily conserved cell reprogramming mechanism, exists in all eukaryotic organisms. It is a fundamental and vital degradation/recycling pathway that removes undesirable components, such as cytoplasmic organelles, misfolded proteins, viruses, and intracellular bacteria, to provide energy and essential materials for organisms. The success of male reproduction depends on healthy testes, which are mainly composed of seminiferous tubules and mesenchyme. Seminiferous tubules are composed of Sertoli cells (SCs) and various germ cells, and the main functional part of mesenchyme are Leydig cells (LCs). In recent years, a large amount of evidence has confirmed that autophagy is active in many cellular events associated with the testes. Autophagy is not only important for testicular spermatogenesis, but is also an essential regulatory mechanism for the ectoplasmic specialization (ES) integrity of SCs, as well as for the normal function of the blood–testes barrier (BTB). At the same time, it is active in LCs and is crucial for steroid production and for maintaining testosterone levels. In this review, we expanded upon the narration regarding the composition of the testes; summarized the regulation and molecular mechanism of autophagy in SCs, germ cells, and LCs; and concluded the roles of autophagy in the process of spermatogenesis and testicular endocrinology. Through integrating the latest summaries and advances, we discuss how the role of autophagy is a double-edged sword in the testes and may provide insight for future studies and explorations on autophagy in male reproduction.     

High molecular weight guar gum assisted settling of fine solids in diluted bitumen:Effect of solvents

As water-based extraction technologies for producing bitumen from oil sands have received increasing environmental concerns,developing non-aqueous extraction (N...     

Combined Metabolomic and Quantitative RT-PCR Analyses Revealed the Synthetic Differences of 2-Acetyl-1-pyrroline in Aromatic and Non-Aromatic Vegetable Soybeans

Aroma is an important economic trait of vegetable soybeans, which greatly influences their market value. The 2-acetyl-1-pyrroline (2AP) is considered as an important substance affecting the aroma of plants. Although the 2AP synthesis pathway has been resolved, the differences of the 2AP synthesis in the aromatic and non-aromatic vegetable soybeans are unknown. In this study, a broad targeted metabolome analysis including measurement of metabolites levels and gene expression levels was performed to reveal pathways of aroma formation in the two developmental stages of vegetable soybean grains [35 (S5) and 40 (S6) days after anthesis] of the ‘Zhexian No. 8’ (ZX8, non-aromatic) and ZK1754 (aromatic). The results showed that the differentially accumulated metabolites (DAMs) of the two varieties can be classified into nine main categories including flavonoids, lipids, amino acids and derivatives, saccharides and alcohols, organic acids, nucleotides and derivatives, phenolic acids, alkaloids and vitamin, which mainly contributed to their phenotypic differences. Furthermore, in combination with the 2AP synthesis pathway, the differences of amino acids and derivatives were mainly involved in the 2AP synthesis. Furthermore, 2AP precursors’ analysis revealed that the accumulation of 2AP mainly occurred from 1-pyrroline-5-carboxylate (P5C), not 4-aminobutyraldehyde (GABald). The quantitative RT-PCR showed that the associated synthetic genes were 1-pyrroline-5-carboxylate dehydrogenase (P5CDH), ∆¹-pyrroline-5-carboxylate synthetase (P5CS), proline dehydrogenase (PRODH) and pyrroline-5-carboxylate reductase (P5CR), which further verified the synthetic pathway of 2AP. Furthermore, the betaine aldehyde dehydrogenase 2 (GmBADH2) mutant was not only vital for the occurrence of 2AP, but also for the synthesis of 4-aminobutyric acid (GABA) in vegetable soybean. Therefore, the differences of 2AP accumulation in aromatic and non-aromatic vegetable soybeans have been revealed, and it also provides an important theoretical basis for aromatic vegetable soybean breeding.