MULTI-WALL CARBON NANOTUBE-BASED DNA NANOSENSOR FOR DETERMINING MITOXANTRONE-DNA INTERACTION IN-VITRO

The present study involves the development of a carbon nanotube based DNA nanosensor to determine the toxicological behavior of mitoxantrone (MTX). Mitoxantrone intercalates with DNA and produces a MTX-DNA adduct, resulting in the blockade of protein synthesis and excessive production of free radicals in the myocardium which eventually leads to cardiac toxicity. So, our work employs a DNA nanosensor to investigate the interaction of MTX with DNA. Multi-wall carbon nanotubes (MWCNTs) were functionalized with carboxyl group and were used for immobilization of DNA to construct the DNA nanosensor. The DNA nanosensor was immersed in MTX solution to monitor MTX-DNA interaction with respect to time and alter the resistance of the nanosensor. It was observed that MTX-DNA interaction is fast initially and as time elapses, the change in interaction gets slow due to formation of MTX-DNA adduct. The limit of detection (LOD) and limit of quantification (LOQ) were found as 150 (ng/mL) and 456 (ng/mL), respectively, for DNA nanosensor. This study suggests that the potentiometric nanosensor allows real-time monitoring of the drug-DNA interaction changes by measuring the potential at DNA/sensor interface which can prove to be an important tool in drug discovery pipelines and molecular toxicology.     

2010 CAV award announcement

The 2010 CAV (Computer-Aided Verification) award was awarded to Kenneth L. McMillan of Cadence Research Laboratories for a series of fundamental contributions resulting in significant advances in scalability of model checking tools. The annual award recognizes a specific fundamental contribution or a series of outstanding contributions to the CAV field.     

Dimensional analysis for planetary mixer: Mixing time and Reynolds numbers

Mixing time number is a convenient parameter to characterize mixing performance of stirred tanks. This dimensionless number is now well established for agitated vessels equipped with vertically and centrally mounted impeller for Newtonian as well as for non-Newtonian fluids. To our knowledge, there is more ambiguity concerning its definition for planetary mixers especially when they have dual motion (around two perpendicular axes) to achieve homogenization. In this study, dimensional analysis of mixing time and reliability of the modified Reynolds and mixing time numbers are proposed for such a planetary mixer particularly named as TRIAXE^® system. These two numbers are based on the maximum tip speed of mixer as the characteristic velocity. Modified dimensionless numbers are consistent with the definition of conventional Reynolds and mixing numbers (when only one revolving motion around the vertical axis of the mixing device occurs in the vessel).Mixing time experiments with TRIAXE^® mixer for highly viscous Newtonian fluids showed that the proposed modified Reynolds and mixing time numbers succeeded to obtain a unique mixing curve irrespective of the different speed ratio chosens. This agreement proves that the proposed modified dimensionless numbers can be well adapted for engineering purposes and they can be used to compare the mixing performance of planetary mixers.     

Evaluation of hot corrosion resistance of HVOF coatings on a Ni-based superalloy in molten salt environment

High-velocity oxy-fuel (HVOF) has the advantage of being a continuous and most convenient process for applying coatings to industrial installations at site. In this study, Cr₃C₂–NiCr, NiCrBSi, Stellite-6 and Ni–20Cr coatings were deposited on a Ni-based superalloy (19.5Cr–3Fe–0.3Ti–0.1C–balance Ni) using an HVOF process. Hot corrosion studies were performed on bare as well as coated superalloy specimens after exposure to a molten salt environment at 900 °C under cyclic conditions. The thermogravimetric technique was used to establish the kinetics of corrosion. XRD, SEM/EDAX and EPMA techniques were used to analyse the corrosion products. The hot corrosion resistance of all the coatings were superior to bare superalloy. Among the coatings studied, the Ni–20Cr coated superalloy imparted maximum hot corrosion resistance, whereas the Stellite-6 coated indicated minimum resistance. The hot corrosion resistance of all coatings may be attributed to the formation of oxides and spinels of nickel, chromium or cobalt.     

Obtaining mode mixity for a bimaterial interface crack using the virtual crack closure technique

We review, unify and extend work pertaining to evaluating mode mixity of interfacial fracture utilizing the virtual crack closure technique (VCCT). From the VCCT, components of the strain energy release rate (SERR) are obtained using the forces and displacements near the crack tip corresponding to the opening and sliding contributions. Unfortunately, these components depend on the crack extension size, Δ, used in the VCCT. It follows that a mode mixity based upon these components also will depend on the crack extension size. However, the components of the strain energy release rate can be used for determining the complex stress intensity factors (SIFs) and the associated mode mixity. In this study, we show that several—seemingly different—suggested methods presented in the literature used to obtain mode mixity based on the stress intensity factors are indeed identical. We also present an alternative, simpler quadratic equation to this end. Moreover, a Δ-independent strain energy release based mode mixity can be defined by introducing a “normalizing length parameter.” We show that when the reference length (used for the SIF-based mode mixity) and the normalizing length (used for Δ-independent SERR-based mode mixity) are equal, the two mode mixities are only shifted by a phase angle, depending on the bimaterial parameter ε.     

Scalable and QoS-Aware Dynamic Slot Assignment and Piconet Partitioning to Enhance the Performance of Bluetooth Ad Hoc Networks

Bluetooth is a radio technology for Wireless Personal Area Networks in the 2.4 GHz ISM frequency band and allows short-range devices to be connected in the form of ad hoc networks. The Bluetooth medium access control protocol is based on a strict master/slave concept wherein any communication between slave devices has to go through the master. While this model is simple, the use of such a nonoptimal packet forwarding scheme incurs much longer delays between any two slave-devices as double the bandwidth is used by the master. In addition, if two or more devices want to communicate as a group, this can only be achieved by either multiple unicast transmissions or a piconet-wide broadcast from the master. To handle these issues efficiently, we propose a novel combination of Dynamic Slot Assignment (DSA) and piconet partitioning. With DSA, the piconet master dynamically assigns slots to slaves so as to allow them to communicate directly with each other without any intervention from the master. Our proposed communication architecture provides for enhanced Quality of Service (QoS), better admission control, and multidevice conversation, which make a multicast-like communication feasible within the piconet. To widen the scope of DSA, we propose a QoS-aware Enhanced DSA (EDSA) version where dynamic piconet partitioning and scatternet support are exploited by grouping devices into piconets as per their connection endpoints, enabling it to be employed over a scatternet. We have performed extensive simulations and observe that these schemes drastically enhance Bluetooth performance in terms of the delay and the throughput, while significantly reducing the network power consumption.     

Effects of Weathering on Treatment of Lead Contaminated Soils

Treatment of lead-contaminated material focuses on the reduction of leached lead concentrations in laboratory leaching tests. There has been little study on the chemical stability of treated materials after waste disposal. To investigate the effects of weathering on several solidification/stabilization chemistries, lead-contaminated soil was treated with various agents (cement, phosphates, dithiocarbamate, calcium hydroxide, calcium carbonate, and metallic iron) and subjected to natural weathering for one year. Lead concentrations and pH values in field percolates and leachates from toxicity characteristic leaching procedure (TCLP) and water leaching tests were used to evaluate changes in the treatment effectiveness. Weathering altered the treatment chemistry of several additives—alkaline additives were neutralized, while reduced additives were oxidized. Lead concentrations in field percolates from the untreated soil were around 1.0?mg/L, and did not vary over one year of weathering time. After one year of weathering, lead concentrations in field percolates from most samples were reduced to low levels (<0.15 mg/L). Weathering also affected lead concentrations in the TCLP and water leach test leachates from the treated wastes. Treated wastes in which the alkaline additives were neutralized or reduced additives oxidized gave higher TCLP lead concentrations after weathering than before, in contrast to the decreasing lead concentrations in the field percolates. Water leaching tests on the alkaline treated wastes had lower lead concentrations after weathering than before.     

Segmented object manufacturing

Rapid Prototyping (RP) is a technology based on a “divide-and-conquer” strategy that enables automatic physical realization of a design without any special tooling. However, existing RP processes suffer from staircase defects since they are all based on 2.5-axis kinematics. To minimize the error due to staircase defects parts are normally built from very thin layers typically with thickness values of 0.010 to 0.300 mm. Therefore, hundreds of layers are required to produce a typical object making RP a slow and costly process. To overcome these limitations, a new RP process called Segmented Object Manufacturing (SOM) is proposed in this paper. SOM makes use of three-axis kinematics in conjunction with a novel slicing method. Slicing in SOM is based on certain visibility-based considerations and is independent of the part accuracy. Since only a few thick layers are used in the SOM technique, a part can be produced faster and cheaper with an accuracy comparable to that of CNC machining.     

Distributed Multi-Level Recovery in Main-Memory Databases

In this paper we present recovery techniques for distributed main-memory databases, specifically for client-server and shared-disk architectures. We present a recovery scheme for client-server architectures which is based on shipping log records to the server, and two recovery schemes for shared-disk architectures—one based on page shipping, and the other based on broadcasting of the log of updates. The schemes offer different tradeoffs, based on factors such as update rates.Our techniques are extensions to a distributed-memory setting of a centralized recovery scheme for main-memory databases, which has been implemented in the Dalì main-memory database system. Our centralized as well as distributed-memory recovery schemes have several attractive features—they support an explicit multi-level recovery abstraction for high concurrency, reduce disk I/O by writing only redo log records to disk during normal processing, and use per-transaction redo and undo logs to reduce contention on the system log. Further, the techniques use a fuzzy checkpointing scheme that writes only dirty pages to disk, yet minimally interferes with normal processing—all but one of our recovery schemes do not require updaters to even acquire a latch before updating a page. Our log shipping/broadcasting schemes also support concurrent updates to the same page at different sites.     

WALRUS: a similarity retrieval algorithm for image databases

Approaches for content-based image querying typically extract a single signature from each image based on color, texture, or shape features. The images returned as the query result are then the ones whose signatures are closest to the signature of the query image. While efficient for simple images, such methods do not work well for complex scenes since they fail to retrieve images that match the query only partially, that is, only certain regions of the image match. This inefficiency leads to the discarding of images that may be semantically very similar to the query image since they may contain the same objects. The problem becomes even more apparent when we consider scaled or translated versions of the similar objects. We propose WALRUS (wavelet-based retrieval of user-specified scenes), a novel similarity retrieval algorithm that is robust to scaling and translation of objects within an image. WALRUS employs a novel similarity model in which each image is first decomposed into its regions and the similarity measure between a pair of images is then defined to be the fraction of the area of the two images covered by matching regions from the images. In order to extract regions for an image, WALRUS considers sliding windows of varying sizes and then clusters them based on the proximity of their signatures. An efficient dynamic programming algorithm is used to compute wavelet-based signatures for the sliding windows. Experimental results on real-life data sets corroborate the effectiveness of WALRUS'S similarity model.