Prof. Dr.-Ing. Hauke Zachert  Ramona Kusch  Andreas Höfgen Dipl.-Ing. 《Bautechnik》2020,97(9):647-655

Planning and construction of large-format dowel shafts at the Hochmosel bridge In the course of the construction of the B 50 neu, the second tallest and longest road bridge in Germany, the Hochmosel bridge, was built in the district of Bernkastel-Wittlich. In the area of the bridge foundations, the subsoil of the Eifel slope is deeply weathered and disturbed and due to an inclination of 25° in average, the normative stability could not be verified with the present geotechnical parameters. In addition, a long lasting measurement program showed a slow but constant movement of the slope by 0.6 mm per year. To prevent such movements and to increase the overall stability of the slope, a slope protection with six massive dowel shafts was designed and installed. These dowel shafts are made of reinforced concrete, reaching down to about 40 m with an outer diameter of 6.6 m and a wall thickness of about 1 m. Installed in two rows between the bridge foundations on the steep slope they are in addition anchored at the head with 55 m long anchors reaching down below the sliding joint. This article highlights geotechnical aspects of the design and gives insights into the construction works and the geology encountered during the excavation work.  相似文献   

    

Franco Leonardo Redondo  Gisela Alejandra Ramona Quiroga  Augusto Gonzaga Oliveira de Freitas  Marcelo Armando Villar  Mario Daniel Ninago  Andrés Eduardo Ciolino 《Polymer-Plastics Technology and Engineering》2020,59(9):985-997

ABSTRACT

In this work, the fabrication of composite coatings based on block copolymers and silicates deposited on stainless-steel plates through electrophoretic deposition technique was studied. Linear poly(?-caprolactone), and poly(dimethylsiloxane-block-ε-caprolactone) copolymers were obtained through ROP polymerization, whereas branched poly(hydroxyethyl methacrylate-co-(hydroxyethylmethacrylate-graft-ε-caprolactone)-block-ε-caprolactone) copolymers were synthesized by a one-pot technique by combining ROP and RAFT synthesis. SEM micrographs revealed homogenous and dense structures over the metallic substrate and the absence of micro-cracks. Thermal characterization revealed residual percentages of solids between 63% and 72% wt/wt. Finally, an in-vitro assessment confirmed the presence of a hydroxyapatite layer over coatings after immersion in a simulated body-fluid solution by XRD analyses.  相似文献   

    

Haomiao Zhang  Thomas Kopfmüller  Ramona Achermann  Jisong Zhang  Andrew Teixeira  Yi Shen  Klavs F. Jensen 《American Institute of Chemical Engineers》2020,66(4):e16873

We present a 3D metal printing showerhead mixer to blend effectively two reagent streams into a confined mixing volume. Each stream is predistributed to multiple channels to increase the contact area in the mixing zone, which enables high mixing performance with smaller pressure drop. The showerhead mixer shows excellent mixing performance owing to its ability to intersperse rapidly the two streams as characterized by the diazo coupling reactions and computational fluid dynamics (CFD) simulations. Experimental results demonstrate superior performance of the showerhead mixer compared to two common commercial micro T-mixers, especially in low Reynolds number regime. CFD results are employed to (a) help understand the mixing mechanism, (b) reproduce the experimental observations, and (c) inform the design specifications for optimal performance. Good agreement between experiments and simulations is achieved. The final design includes multiple side-fed inlets for improved mixing performance of the showerhead mixer, as suggested by the validated CFD models.  相似文献   

    

Julie Zimmermann  Signe Tandrup Schmidt  Ramona Trebbien  Rebecca Jane Cox  Fan Zhou  Frank Follmann  Gabriel Kristian Pedersen  Dennis Christensen 《International journal of molecular sciences》2022,23(3)

The SARS-CoV-2 pandemic caused a massive health and societal crisis, although the fast development of effective vaccines reduced some of the impact. To prepare for future respiratory virus pandemics, a pan-viral prophylaxis could be used to control the initial virus outbreak in the period prior to vaccine approval. The liposomal vaccine adjuvant CAF^®09b contains the TLR3 agonist polyinosinic:polycytidylic acid, which induces a type I interferon (IFN-I) response and an antiviral state in the affected tissues. When testing CAF09b liposomes as a potential pan-viral prophylaxis, we observed that intranasal administration of CAF09b liposomes to mice resulted in an influx of innate immune cells into the nose and lungs and upregulation of IFN-I-related gene expression. When CAF09b liposomes were administered prior to challenge with mouse-adapted influenza A/Puerto Rico/8/1934 virus, it protected from severe disease, although the virus was still detectable in the lungs. However, when CAF09b liposomes were administered after influenza challenge, the mice had a similar disease course to controls. In conclusion, CAF09b may be a suitable candidate as a pan-viral prophylactic treatment for epidemic viruses, but must be administered prior to virus exposure to be effective.  相似文献   

    

Tiziana Genovese  Marika Cordaro  Rosalba Siracusa  Daniela Impellizzeri  Sebastiano Caudullo  Emanuela Raffone  Francesco Macrí  Livia Interdonato  Enrico Gugliandolo  Claudia Interlandi  Rosalia Crupi  Ramona D&#x;Amico  Roberta Fusco  Salvatore Cuzzocrea  Rosanna Di Paola 《International journal of molecular sciences》2022,23(10)

Endometriosis is usually associated with inflammation and chronic pelvic pain. This paper focuses the attention on the anti-inflammatory, anti-oxidant and analgesic effects of cannabidiol (CBD) and on its potential role in endometriosis. We employed an in vivo model of endometriosis and administered CBD daily by gavage. CBD administration strongly reduced lesions diameter, volume and area. In particular, it was able to modify lesion morphology, reducing epithelial glands and stroma. CBD showed anti-oxidant effects reducing lipid peroxidation, the expression of Nox-1 and Nox-4 enzymes. CBD restored the oxidative equilibrium of the endogenous cellular defense as showed by the SOD activity and the GSH levels in the lesions. CBD also showed important antifibrotic effects as showed by the Masson trichrome staining and by downregulated expression of MMP-9, iNOS and TGF-β. CBD was able to reduce inflammation both in the harvested lesions, as showed by the increased Ikb-α and reduced COX2 cytosolic expressions and reduced NFkB nuclear localization, and in the peritoneal fluids as showed by the decreased TNF-α, PGE2 and IL-1α levels. CBD has important analgesic effects as showed by the reduced mast cells recruitment in the spinal cord and the reduced release of neuro-sensitizing and pro-inflammatory mediators. In conclusion, the collected data showed that CBD has an effective and coordinated effects in endometriosis suppression.  相似文献   

    

Oana L. Pop  Dan C. Vodnar  Ramona Suharoschi  Elena Mudura  Carmen Socaciu 《Journal of food process engineering》2016,39(5):433-441

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Ramona Oana Gunache  Constantin Apetrei 《International journal of molecular sciences》2021,22(14)

In this paper, the electrochemical behavior of two types of sensors based on modified screen-printed electrodes (one screen-printed electrode based on carbon (SPCE) and another screen-printed electrode modified with Prussian Blue (PB/SPCE)) was studied with the aim of sensitive detection of diosmin, an active pharmaceutical compound from the class of flavonoids. The scan electron microscopy technique was used for the morphological characterization of PB/SPCE. The preliminary analysis assessed the electrochemical behavior of SPCE and PB/SPCE in KCl solution and in a double solution of potassium ferrocyanide–potassium chloride. It was shown that the active area of PB/SPCE is superior to the one of SPCE, the greater sensitivity being related with the presence of the electroactive modifier. Similarly, in the case of diosmin detection, the PB/SPCE sensor detect more sensitivity the diosmin due to the electrocatalytic effect of PB. From the study of the influence of reaction rate on the sensor’s electrochemical response, it was shown that the detection process is controlled by the adsorption process, the degree of surface coverage with electroactive molecules being higher in the case of PB/SPCE. From the PB/SPCE calibration curve, it wasdetermined that it has high sensitivity and low detection and quantification limit values (limit of detection 5.22 × 10⁻⁸ M). The applicability of the PB/SPCE sensor was confirmed by sensitive analysis of diosmin in pharmaceutical products. The voltammetric method is suitable for the detection and quantification of diosmin in pharmaceutical products. The method is simple, accurate, and quick and can be used in routine analysis in the examination of the quality of pharmaceutical products and other types of samples.  相似文献   

    

Ramona D&#x;Amico  Francesco Monaco  Rosalba Siracusa  Marika Cordaro  Roberta Fusco  Alessio Filippo Peritore  Enrico Gugliandolo  Rosalia Crupi  Salvatore Cuzzocrea  Rosanna Di Paola  Daniela Impellizzeri  Tiziana Genovese 《International journal of molecular sciences》2021,22(21)

Disseminated intravascular coagulation (DIC) is a severe condition characterized by the systemic formation of microthrombi complicated with bleeding tendency and organ dysfunction. In the last years, it represents one of the most frequent consequences of coronavirus disease 2019 (COVID-19). The pathogenesis of DIC is complex, with cross-talk between the coagulant and inflammatory pathways. The objective of this study is to investigate the anti-inflammatory action of ultramicronized palmitoylethanolamide (um-PEA) in a lipopolysaccharide (LPS)-induced DIC model in rats. Experimental DIC was induced by continual infusion of LPS (30 mg/kg) for 4 h through the tail vein. Um-PEA (30 mg/kg) was given orally 30 min before and 1 h after the start of intravenous infusion of LPS. Results showed that um-PEA reduced alteration of coagulation markers, as well as proinflammatory cytokine release in plasma and lung samples, induced by LPS infusion. Furthermore, um-PEA also has the effect of preventing the formation of fibrin deposition and lung damage. Moreover, um-PEA was able to reduce the number of mast cells (MCs) and the release of its serine proteases, which are also necessary for SARS-CoV-2 infection. These results suggest that um-PEA could be considered as a potential therapeutic approach in the management of DIC and in clinical implications associated to coagulopathy and lung dysfunction, such as COVID-19.  相似文献   

    

Rosalba Siracusa  Francesco Monaco  Ramona D&#x;Amico  Tiziana Genovese  Marika Cordaro  Livia Interdonato  Enrico Gugliandolo  Alessio Filippo Peritore  Rosalia Crupi  Salvatore Cuzzocrea  Daniela Impellizzeri  Roberta Fusco  Rosanna Di Paola 《International journal of molecular sciences》2021,22(13)

Treating postoperative (PO) pain is a clinical challenge. Inadequate PO pain management can lead to worse outcomes, for example chronic post-surgical pain. Therefore, acquiring new information on the PO pain mechanism would increase the therapeutic options available. In this paper, we evaluated the role of a natural substance, epigallocatechin-3-gallate (EGCG), on pain and neuroinflammation induced by a surgical procedure in an animal model of PO pain. We performed an incision of the hind paw and EGCG was administered for five days. Mechanical allodynia, thermal hyperalgesia, and motor dysfunction were assessed 24 h, and three and five days after surgery. At the same time points, animals were sacrificed, and sera and lumbar spinal cord tissues were harvested for molecular analysis. EGCG administration significantly alleviated hyperalgesia and allodynia, and reduced motor disfunction. From the molecular point of view, EGCG reduced the activation of the WNT pathway, reducing WNT3a, cysteine-rich domain frizzled (FZ)1 and FZ8 expressions, and both cytosolic and nuclear β-catenin expression, and the noncanonical β-catenin–independent signaling pathways, reducing the activation of the NMDA receptor subtype NR2B (pNR2B), pPKC and cAMP response element-binding protein (pCREB) expressions at all time points. Additionally, EGCG reduced spinal astrocytes and microglia activation, cytokines overexpression and nuclear factor kappa-light-chain-enhancer of activated B cells (NFkB) pathway, downregulating inducible nitric oxide synthase (iNOS) activation, cyclooxygenase 2 (COX-2) expression, and prostaglandin E2 (PGE2) levels. Thus, EGCG administration managing the WNT/β-catenin signaling pathways modulates PO pain related neurochemical and inflammatory alterations.  相似文献   

    

Alessio Filippo Peritore  Ramona D&#x;Amico  Rosalba Siracusa  Marika Cordaro  Roberta Fusco  Enrico Gugliandolo  Tiziana Genovese  Rosalia Crupi  Rosanna Di Paola  Salvatore Cuzzocrea  Daniela Impellizzeri 《International journal of molecular sciences》2021,22(11)

Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are common and devastating clinical disorders with high mortality and no specific therapy. Lipopolysaccharide (LPS) is usually used intratracheally to induce ALI in mice. The aim of this study was to examine the effects of an ultramicronized preparation of palmitoylethanolamide (um-PEA) in mice subjected to LPS-induced ALI. Histopathological analysis reveals that um-PEA reduced alteration in lung after LPS intratracheal administration. Besides, um-PEA decreased wet/dry weight ratio and myeloperoxidase, a marker of neutrophils infiltration, macrophages and total immune cells number and mast cells degranulation in lung. Moreover, um-PEA could also decrease cytokines release of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and interleukin (IL)-18. Furthermore, um-PEA significantly inhibited the phosphorylation of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation in ALI, and at the same time decreased extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38/MAPK) expression, that was increased after LPS administration. Our study suggested that um-PEA contrasted LPS-induced ALI, exerting its potential role as an adjuvant anti-inflammatory therapeutic for treating lung injury, maybe also by p38/NF-κB pathway.  相似文献